Restriction map for
pF1KB7228
(Linear) (Six-base) MAPSORT of: KIBB7228.seq Check: 7036 from: 1 to: 1785
KIBB7228.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
Acc65I G'GTAC_C
Cuts at: 0 772 1785
Size: 772 1013
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1785
Size: 892 893
AflIII A'CryG_T
Cuts at: 0 706 718 1785
Size: 706 12 1067
Fragments arranged by size:
1067 706 12
AhdI GACnn_n'nnGTC
Cuts at: 0 1666 1785
Size: 1666 119
ApoI r'AATT_y
Cuts at: 0 4 306 632 1276 1785
Size: 4 302 326 644 509
Fragments arranged by size:
644 509 326 302 4
AvrII C'CTAG_G
Cuts at: 0 921 1785
Size: 921 864
BanI G'GyrC_C
Cuts at: 0 772 1785
Size: 772 1013
BanII G_rGCy'C
Cuts at: 0 1758 1785
Size: 1758 27
BbvCI CC'TCA_GC
Cuts at: 0 989 1785
Size: 989 796
BciVI GTATCCnnnnn_n'
Cuts at: 0 604 1785
Size: 604 1181
BlpI GC'TnA_GC
Cuts at: 0 412 1785
Size: 412 1373
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 341 1785
Size: 341 1444
Bpu10I CC'TnA_GC
Cuts at: 0 989 1785
Size: 989 796
BsaI GGTCTCn'nnnn_
Cuts at: 0 1085 1768 1785
Size: 1085 683 17
Fragments arranged by size:
1085 683 17
BsaAI yAC'GTr
Cuts at: 0 702 709 719 1785
Size: 702 7 10 1066
Fragments arranged by size:
1066 702 10 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1223 1253 1475 1505 1785
Size: 1223 30 222 30 280
Fragments arranged by size:
1223 280 222 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1363 1379 1785
Size: 1363 16 406
Fragments arranged by size:
1363 406 16
BseYI C'CCAG_C
Cuts at: 0 372 867 1197 1785
Size: 372 495 330 588
Fragments arranged by size:
588 495 372 330
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1103 1785
Size: 484 619 682
Fragments arranged by size:
682 619 484
BsmI GAATG_Cn'
Cuts at: 0 1576 1785
Size: 1576 209
Bsp1286I G_dGCh'C
Cuts at: 0 1758 1785
Size: 1758 27
BspHI T'CATG_A
Cuts at: 0 380 1785
Size: 380 1405
BsrDI GCAATG_nn'
Cuts at: 0 154 446 728 1785
Size: 154 292 282 1057
Fragments arranged by size:
1057 292 282 154
BstAPI GCAn_nnn'nTGC
Cuts at: 0 109 1785
Size: 109 1676
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1018 1785
Size: 1018 767
BstYI r'GATC_y
Cuts at: 0 899 1785
Size: 899 886
BtgI C'CryG_G
Cuts at: 0 434 534 1785
Size: 434 100 1251
Fragments arranged by size:
1251 434 100
BtsI GCAGTG_nn'
Cuts at: 0 389 1785
Size: 389 1396
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 626 661 1785
Size: 626 35 1124
Fragments arranged by size:
1124 626 35
EaeI y'GGCC_r
Cuts at: 0 1511 1785
Size: 1511 274
EarI CTCTTCn'nnn_
Cuts at: 0 46 744 1063 1785
Size: 46 698 319 722
Fragments arranged by size:
722 698 319 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 1785
Size: 892 893
EcoRI G'AATT_C
Cuts at: 0 1276 1785
Size: 1276 509
EcoRV GAT'ATC
Cuts at: 0 1427 1785
Size: 1427 358
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1678 1785
Size: 1678 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1652 1684 1785
Size: 1652 32 101
Fragments arranged by size:
1652 101 32
HincII GTy'rAC
Cuts at: 0 1671 1785
Size: 1671 114
HindIII A'AGCT_T
Cuts at: 0 980 1015 1785
Size: 980 35 770
Fragments arranged by size:
980 770 35
KpnI G_GTAC'C
Cuts at: 0 776 1785
Size: 776 1009
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 996 1785
Size: 632 364 789
Fragments arranged by size:
789 632 364
MslI CAynn'nnrTG
Cuts at: 0 439 505 1066 1525 1645 1785
Size: 439 66 561 459 120 140
Fragments arranged by size:
561 459 439 140 120 66
MspA1I CmG'CkG
Cuts at: 0 871 1785
Size: 871 914
NcoI C'CATG_G
Cuts at: 0 434 1785
Size: 434 1351
NdeI CA'TA_TG
Cuts at: 0 1623 1705 1785
Size: 1623 82 80
Fragments arranged by size:
1623 82 80
PasI CC'CwG_GG
Cuts at: 0 1295 1785
Size: 1295 490
PflMI CCAn_nnn'nTGG
Cuts at: 0 140 1785
Size: 140 1645
PfoI T'CCnGG_A
Cuts at: 0 606 895 1785
Size: 606 289 890
Fragments arranged by size:
890 606 289
PmlI CAC'GTG
Cuts at: 0 702 1785
Size: 702 1083
PshAI GACnn'nnGTC
Cuts at: 0 1051 1785
Size: 1051 734
PsiI TTA'TAA
Cuts at: 0 1066 1785
Size: 1066 719
PvuII CAG'CTG
Cuts at: 0 871 1785
Size: 871 914
SfcI C'TryA_G
Cuts at: 0 458 1148 1226 1785
Size: 458 690 78 559
Fragments arranged by size:
690 559 458 78
SspI AAT'ATT
Cuts at: 0 1499 1785
Size: 1499 286
StyI C'CwwG_G
Cuts at: 0 95 434 921 1049 1785
Size: 95 339 487 128 736
Fragments arranged by size:
736 487 339 128 95
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1617 1785
Size: 1617 168
TatI w'GTAC_w
Cuts at: 0 1564 1708 1785
Size: 1564 144 77
Fragments arranged by size:
1564 144 77
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1475 1507 1785
Size: 1475 32 278
Fragments arranged by size:
1475 278 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1265 1785
Size: 1265 520
XmnI GAAnn'nnTTC
Cuts at: 0 7 1276 1520 1785
Size: 7 1269 244 265
Fragments arranged by size:
1269 265 244 7
Enzymes that do cut:
Acc65I AcuI AflIII AhdI ApoI AvrII BanI BanII
BbvCI BciVI BlpI BplI Bpu10I BsaI BsaAI BsaXI
BseRI BseYI BsgI BsmI Bsp1286I BspHI BsrDI BstAPI
BstXI BstYI BtgI BtsI CspCI EaeI EarI Eco57MI
EcoRI EcoRV FalI Hin4I HincII HindIII KpnI MmeI
MslI MspA1I NcoI NdeI PasI PflMI PfoI PmlI
PshAI PsiI PvuII SfcI SspI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI BaeI BamHI BbeI BbsI BcgI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BmtI BpmI BpuEI
BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoO109I FseI FspI FspAI HaeII
HpaI KasI MfeI MluI MscI NaeI NarI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI StuI SwaI Tth111I XbaI XhoI XmaI
ZraI