Restriction map for
pF1KB7231
(Linear) (Six-base) MAPSORT of: KIBB7231.seq Check: 5615 from: 1 to: 1302
KIBB7231.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 131 1302
Size: 131 1171
Acc65I G'GTAC_C
Cuts at: 0 442 1302
Size: 442 860
AclI AA'CG_TT
Cuts at: 0 1159 1302
Size: 1159 143
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 1302
Size: 237 1065
AfeI AGC'GCT
Cuts at: 0 617 1302
Size: 617 685
AflIII A'CryG_T
Cuts at: 0 176 1302
Size: 176 1126
AhdI GACnn_n'nnGTC
Cuts at: 0 1251 1302
Size: 1251 51
AleI CACnn'nnGTG
Cuts at: 0 535 1302
Size: 535 767
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 160 1302
Size: 160 1142
ApaI G_GGCC'C
Cuts at: 0 239 1302
Size: 239 1063
ApaLI G'TGCA_C
Cuts at: 0 145 1302
Size: 145 1157
ApoI r'AATT_y
Cuts at: 0 1216 1302
Size: 1216 86
AvaI C'yCGr_G
Cuts at: 0 231 1302
Size: 231 1071
BanI G'GyrC_C
Cuts at: 0 49 100 211 442 1302
Size: 49 51 111 231 860
Fragments arranged by size:
860 231 111 51 49
BanII G_rGCy'C
Cuts at: 0 239 545 866 1302
Size: 239 306 321 436
Fragments arranged by size:
436 321 306 239
BbeI G_GCGC'C
Cuts at: 0 53 1302
Size: 53 1249
BbsI GAAGACnn'nnnn_
Cuts at: 0 243 1302
Size: 243 1059
BglI GCCn_nnn'nGGC
Cuts at: 0 243 1302
Size: 243 1059
BglII A'GATC_T
Cuts at: 0 461 1302
Size: 461 841
BlpI GC'TnA_GC
Cuts at: 0 860 1302
Size: 860 442
Bme1580I G_kGCm'C
Cuts at: 0 149 214 239 1302
Size: 149 65 25 1063
Fragments arranged by size:
1063 149 65 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 309 437 1112 1302
Size: 309 128 675 190
Fragments arranged by size:
675 309 190 128
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 87 1302
Size: 87 1215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 223 1302
Size: 151 72 1079
Fragments arranged by size:
1079 151 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1114 1302
Size: 1114 188
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 1143 1302
Size: 586 557 159
Fragments arranged by size:
586 557 159
BsaAI yAC'GTr
Cuts at: 0 177 713 1302
Size: 177 536 589
Fragments arranged by size:
589 536 177
BsaHI Gr'CG_yC
Cuts at: 0 50 1302
Size: 50 1252
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1283 1302
Size: 1283 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 484 1302
Size: 484 818
BseYI C'CCAG_C
Cuts at: 0 869 1302
Size: 869 433
BsiEI CG_ry'CG
Cuts at: 0 924 1286 1302
Size: 924 362 16
Fragments arranged by size:
924 362 16
BsiHKAI G_wGCw'C
Cuts at: 0 17 149 194 866 1302
Size: 17 132 45 672 436
Fragments arranged by size:
672 436 132 45 17
BsmI GAATG_Cn'
Cuts at: 0 744 1302
Size: 744 558
Bsp1286I G_dGCh'C
Cuts at: 0 17 149 194 214 239 545 866 1302
Size: 17 132 45 20 25 306 321 436
Fragments arranged by size:
436 321 306 132 45 25 20 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 131 161 237 755 1302
Size: 131 30 76 518 547
Fragments arranged by size:
547 518 131 76 30
BssSI C'ACGA_G
Cuts at: 0 154 193 1302
Size: 154 39 1109
Fragments arranged by size:
1109 154 39
BstEII G'GTnAC_C
Cuts at: 0 667 705 1302
Size: 667 38 597
Fragments arranged by size:
667 597 38
BstXI CCAn_nnnn'nTGG
Cuts at: 0 768 1302
Size: 768 534
BstYI r'GATC_y
Cuts at: 0 198 461 1302
Size: 198 263 841
Fragments arranged by size:
841 263 198
BtgI C'CryG_G
Cuts at: 0 108 215 533 1280 1302
Size: 108 107 318 747 22
Fragments arranged by size:
747 318 108 107 22
BtsI GCAGTG_nn'
Cuts at: 0 37 1302
Size: 37 1265
DraIII CAC_nnn'GTG
Cuts at: 0 684 713 1302
Size: 684 29 589
Fragments arranged by size:
684 589 29
EaeI y'GGCC_r
Cuts at: 0 758 921 1283 1302
Size: 758 163 362 19
Fragments arranged by size:
758 362 163 19
EagI C'GGCC_G
Cuts at: 0 921 1283 1302
Size: 921 362 19
Fragments arranged by size:
921 362 19
EcoICRI GAG'CTC
Cuts at: 0 864 1302
Size: 864 438
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 223 237 1302
Size: 151 72 14 1065
Fragments arranged by size:
1065 151 72 14
EcoO109I rG'GnC_Cy
Cuts at: 0 235 236 638 1302
Size: 235 1 402 664
Fragments arranged by size:
664 402 235 1
EcoRI G'AATT_C
Cuts at: 0 1216 1302
Size: 1216 86
HaeII r_GCGC'y
Cuts at: 0 53 619 1302
Size: 53 566 683
Fragments arranged by size:
683 566 53
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1237 1269 1302
Size: 1237 32 33
Fragments arranged by size:
1237 33 32
KasI G'GCGC_C
Cuts at: 0 49 1302
Size: 49 1253
KpnI G_GTAC'C
Cuts at: 0 446 1302
Size: 446 856
MscI TGG'CCA
Cuts at: 0 760 1302
Size: 760 542
MslI CAynn'nnrTG
Cuts at: 0 142 535 766 964 1302
Size: 142 393 231 198 338
Fragments arranged by size:
393 338 231 198 142
MspA1I CmG'CkG
Cuts at: 0 97 129 164 402 735 1302
Size: 97 32 35 238 333 567
Fragments arranged by size:
567 333 238 97 35 32
NarI GG'CG_CC
Cuts at: 0 50 1302
Size: 50 1252
NcoI C'CATG_G
Cuts at: 0 215 1302
Size: 215 1087
NdeI CA'TA_TG
Cuts at: 0 628 1302
Size: 628 674
NspI r_CATG'y
Cuts at: 0 85 963 1302
Size: 85 878 339
Fragments arranged by size:
878 339 85
PasI CC'CwG_GG
Cuts at: 0 414 1073 1302
Size: 414 659 229
Fragments arranged by size:
659 414 229
PflMI CCAn_nnn'nTGG
Cuts at: 0 59 414 1302
Size: 59 355 888
Fragments arranged by size:
888 355 59
PfoI T'CCnGG_A
Cuts at: 0 634 1121 1302
Size: 634 487 181
Fragments arranged by size:
634 487 181
PmlI CAC'GTG
Cuts at: 0 713 1302
Size: 713 589
PpuMI rG'GwC_Cy
Cuts at: 0 638 1302
Size: 638 664
PshAI GACnn'nnGTC
Cuts at: 0 914 1302
Size: 914 388
PsiI TTA'TAA
Cuts at: 0 490 1302
Size: 490 812
PspOMI G'GGCC_C
Cuts at: 0 235 1302
Size: 235 1067
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1302
Size: 1000 32 270
Fragments arranged by size:
1000 270 32
PstI C_TGCA'G
Cuts at: 0 128 392 1302
Size: 128 264 910
Fragments arranged by size:
910 264 128
PvuII CAG'CTG
Cuts at: 0 129 402 735 1302
Size: 129 273 333 567
Fragments arranged by size:
567 333 273 129
SacI G_AGCT'C
Cuts at: 0 866 1302
Size: 866 436
SbfI CC_TGCA'GG
Cuts at: 0 392 1302
Size: 392 910
SexAI A'CCwGG_T
Cuts at: 0 680 1302
Size: 680 622
SfcI C'TryA_G
Cuts at: 0 124 388 1302
Size: 124 264 914
Fragments arranged by size:
914 264 124
SfoI GGC'GCC
Cuts at: 0 51 1302
Size: 51 1251
SmlI C'TyrA_G
Cuts at: 0 1129 1302
Size: 1129 173
SphI G_CATG'C
Cuts at: 0 963 1302
Size: 963 339
StuI AGG'CCT
Cuts at: 0 1174 1302
Size: 1174 128
StyI C'CwwG_G
Cuts at: 0 215 305 518 1302
Size: 215 90 213 784
Fragments arranged by size:
784 215 213 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 406 672 883 1302
Size: 406 266 211 419
Fragments arranged by size:
419 406 266 211
Tth111I GACn'n_nGTC
Cuts at: 0 339 355 429 1302
Size: 339 16 74 873
Fragments arranged by size:
873 339 74 16
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 415 972 1302
Size: 415 557 330
Fragments arranged by size:
557 415 330
Enzymes that do cut:
AarI Acc65I AclI AcuI AfeI AflIII AhdI AleI
AlfI ApaI ApaLI ApoI AvaI BanI BanII BbeI
BbsI BglI BglII BlpI Bme1580I BmrI BplI BpmI
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BssSI BstEII BstXI BstYI
BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoO109I
EcoRI HaeII Hin4I KasI KpnI MscI MslI MspA1I
NarI NcoI NdeI NspI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuII SacI
SbfI SexAI SfcI SfoI SmlI SphI StuI StyI
TaqII Tth111I XcmI
Enzymes that do not cut:
AatII AccI AflII AgeI AloI AlwNI AscI AseI
AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI
BfrBI BmgBI BmtI Bpu10I BsaBI BsaWI BsgI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EarI EciI EcoNI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI MfeI MluI MmeI NaeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PspXI PvuI RsrII SacII SalI SanDI SapI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI TstI XbaI XhoI XmaI XmnI
ZraI