Restriction map for
pF1KB7233
(Linear) (Six-base) MAPSORT of: KIBB7233.seq Check: 6085 from: 1 to: 1347
KIBB7233.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AccI GT'mk_AC
Cuts at: 0 175 1347
Size: 175 1172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 917 1347
Size: 712 205 430
Fragments arranged by size:
712 430 205
AfeI AGC'GCT
Cuts at: 0 589 1347
Size: 589 758
AgeI A'CCGG_T
Cuts at: 0 1306 1347
Size: 1306 41
AhdI GACnn_n'nnGTC
Cuts at: 0 453 1347
Size: 453 894
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 401 925 1347
Size: 401 524 422
Fragments arranged by size:
524 422 401
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 865 866 897 898 1347
Size: 865 1 31 1 449
Fragments arranged by size:
865 449 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 200 1167 1347
Size: 200 967 180
Fragments arranged by size:
967 200 180
AvaI C'yCGr_G
Cuts at: 0 409 1347
Size: 409 938
BanI G'GyrC_C
Cuts at: 0 92 1347
Size: 92 1255
BanII G_rGCy'C
Cuts at: 0 482 794 834 1347
Size: 482 312 40 513
Fragments arranged by size:
513 482 312 40
BglI GCCn_nnn'nGGC
Cuts at: 0 145 1347
Size: 145 1202
Bme1580I G_kGCm'C
Cuts at: 0 445 1347
Size: 445 902
BmgBI CAC'GTC
Cuts at: 0 1176 1347
Size: 1176 171
BmrI ACTGGGnnnn_n'
Cuts at: 0 885 1347
Size: 885 462
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 723 1347
Size: 723 624
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 376 394 415 466 598 691 982
Size: 74 302 18 21 51 132 93 291
Cuts at: 982 1009 1159 1243 1347
Size: 27 150 84 104
Fragments arranged by size:
302 291 150 132 104 93 84 74
51 27 21 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1347
Size: 697 650
BsaI GGTCTCn'nnnn_
Cuts at: 0 310 391 847 1319 1347
Size: 310 81 456 472 28
Fragments arranged by size:
472 456 310 81 28
BsaWI w'CCGG_w
Cuts at: 0 770 1211 1306 1347
Size: 770 441 95 41
Fragments arranged by size:
770 441 95 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 33 34 63 925 955 1347
Size: 4 29 1 29 862 30 392
Fragments arranged by size:
862 392 30 29 29 4 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 12 397 429 490 712 762 946 1347
Size: 12 385 32 61 222 50 184 401
Fragments arranged by size:
401 385 222 184 61 50 32 12
BseYI C'CCAG_C
Cuts at: 0 193 677 756 1007 1347
Size: 193 484 79 251 340
Fragments arranged by size:
484 340 251 193 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 1347
Size: 519 828
BsiHKAI G_wGCw'C
Cuts at: 0 482 1347
Size: 482 865
BsmI GAATG_Cn'
Cuts at: 0 390 519 987 1347
Size: 390 129 468 360
Fragments arranged by size:
468 390 360 129
Bsp1286I G_dGCh'C
Cuts at: 0 445 482 794 834 1347
Size: 445 37 312 40 513
Fragments arranged by size:
513 445 312 40 37
BspEI T'CCGG_A
Cuts at: 0 770 1347
Size: 770 577
BsrBI CCG'CTC
Cuts at: 0 1143 1347
Size: 1143 204
BsrFI r'CCGG_y
Cuts at: 0 1306 1347
Size: 1306 41
BsrGI T'GTAC_A
Cuts at: 0 528 1347
Size: 528 819
BssSI C'ACGA_G
Cuts at: 0 427 724 1347
Size: 427 297 623
Fragments arranged by size:
623 427 297
BstXI CCAn_nnnn'nTGG
Cuts at: 0 354 1347
Size: 354 993
BstYI r'GATC_y
Cuts at: 0 491 956 1124 1347
Size: 491 465 168 223
Fragments arranged by size:
491 465 223 168
BtgI C'CryG_G
Cuts at: 0 44 845 1326 1347
Size: 44 801 481 21
Fragments arranged by size:
801 481 44 21
BtsI GCAGTG_nn'
Cuts at: 0 221 946 1347
Size: 221 725 401
Fragments arranged by size:
725 401 221
ClaI AT'CG_AT
Cuts at: 0 83 1347
Size: 83 1264
DraIII CAC_nnn'GTG
Cuts at: 0 440 1347
Size: 440 907
EaeI y'GGCC_r
Cuts at: 0 137 303 344 656 1323 1347
Size: 137 166 41 312 667 24
Fragments arranged by size:
667 312 166 137 41 24
EarI CTCTTCn'nnn_
Cuts at: 0 1103 1347
Size: 1103 244
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 628 1347
Size: 628 719
EcoICRI GAG'CTC
Cuts at: 0 480 1347
Size: 480 867
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 376 394 415 466 598 691 712
Size: 74 302 18 21 51 132 93 21
Cuts at: 712 917 982 1009 1159 1243 1347
Size: 205 65 27 150 84 104
Fragments arranged by size:
302 205 150 132 104 93 84 74
65 51 27 21 21 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 358 658 694 1347
Size: 358 300 36 653
Fragments arranged by size:
653 358 300 36
EcoO109I rG'GnC_Cy
Cuts at: 0 242 278 1347
Size: 242 36 1069
Fragments arranged by size:
1069 242 36
HaeII r_GCGC'y
Cuts at: 0 591 1002 1347
Size: 591 411 345
Fragments arranged by size:
591 411 345
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 816 848 1102 1134 1347
Size: 439 32 345 32 254 32 213
Fragments arranged by size:
439 345 254 213 32 32 32
HincII GTy'rAC
Cuts at: 0 176 1347
Size: 176 1171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1169 1347
Size: 932 237 178
Fragments arranged by size:
932 237 178
MscI TGG'CCA
Cuts at: 0 139 305 346 658 1325 1347
Size: 139 166 41 312 667 22
Fragments arranged by size:
667 312 166 139 41 22
MslI CAynn'nnrTG
Cuts at: 0 866 1347
Size: 866 481
MspA1I CmG'CkG
Cuts at: 0 372 603 756 1347
Size: 372 231 153 591
Fragments arranged by size:
591 372 231 153
NcoI C'CATG_G
Cuts at: 0 44 845 1326 1347
Size: 44 801 481 21
Fragments arranged by size:
801 481 44 21
PasI CC'CwG_GG
Cuts at: 0 183 1347
Size: 183 1164
PflMI CCAn_nnn'nTGG
Cuts at: 0 102 606 870 1294 1347
Size: 102 504 264 424 53
Fragments arranged by size:
504 424 264 102 53
PfoI T'CCnGG_A
Cuts at: 0 51 638 959 1347
Size: 51 587 321 388
Fragments arranged by size:
587 388 321 51
PpuMI rG'GwC_Cy
Cuts at: 0 242 1347
Size: 242 1105
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1141 1173 1347
Size: 1141 32 174
Fragments arranged by size:
1141 174 32
PstI C_TGCA'G
Cuts at: 0 914 941 1034 1347
Size: 914 27 93 313
Fragments arranged by size:
914 313 93 27
PvuII CAG'CTG
Cuts at: 0 372 603 756 1347
Size: 372 231 153 591
Fragments arranged by size:
591 372 231 153
SacI G_AGCT'C
Cuts at: 0 482 1347
Size: 482 865
SalI G'TCGA_C
Cuts at: 0 174 1347
Size: 174 1173
SapI GCTCTTCn'nnn_
Cuts at: 0 1103 1347
Size: 1103 244
SbfI CC_TGCA'GG
Cuts at: 0 914 1347
Size: 914 433
SexAI A'CCwGG_T
Cuts at: 0 1160 1347
Size: 1160 187
SfcI C'TryA_G
Cuts at: 0 12 910 937 1030 1347
Size: 12 898 27 93 317
Fragments arranged by size:
898 317 93 27 12
SgrAI Cr'CCGG_yG
Cuts at: 0 1306 1347
Size: 1306 41
SmlI C'TyrA_G
Cuts at: 0 409 712 1347
Size: 409 303 635
Fragments arranged by size:
635 409 303
StyI C'CwwG_G
Cuts at: 0 44 659 845 1326 1347
Size: 44 615 186 481 21
Fragments arranged by size:
615 481 186 44 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 80 1347
Size: 80 1267
TatI w'GTAC_w
Cuts at: 0 528 1276 1347
Size: 528 748 71
Fragments arranged by size:
748 528 71
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 33 65 213 245 925 957 1347
Size: 33 32 148 32 680 32 390
Fragments arranged by size:
680 390 148 33 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 132 666 1347
Size: 132 534 681
Fragments arranged by size:
681 534 132
XhoI C'TCGA_G
Cuts at: 0 409 1347
Size: 409 938
Enzymes that do cut:
AccI AcuI AfeI AgeI AhdI AlfI AloI AlwNI
AvaI BanI BanII BglI Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrFI BsrGI BssSI
BstXI BstYI BtgI BtsI ClaI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
MmeI MscI MslI MspA1I NcoI PasI PflMI PfoI
PpuMI PsrI PstI PvuII SacI SalI SapI SbfI
SexAI SfcI SgrAI SmlI StyI TaqII TatI TstI
XcmI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AleI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI Bpu10I BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BspHI BspMI BsrDI BssHII BstAPI BstBI BstEII
BstZ17I Bsu36I BtgZI CspCI DraI DrdI EagI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacII
SanDI ScaI SfiI SfoI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XmaI
XmnI ZraI