Restriction map for
pF1KB7234
(Linear) (Six-base) MAPSORT of: KIBB7234.seq Check: 7129 from: 1 to: 1164
KIBB7234.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 136 1164
Size: 136 1028
AatII G_ACGT'C
Cuts at: 0 958 1164
Size: 958 206
AccI GT'mk_AC
Cuts at: 0 923 1164
Size: 923 241
Acc65I G'GTAC_C
Cuts at: 0 473 1164
Size: 473 691
AflIII A'CryG_T
Cuts at: 0 730 1164
Size: 730 434
AleI CACnn'nnGTG
Cuts at: 0 721 1164
Size: 721 443
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 911 1164
Size: 911 253
AlwNI CAG_nnn'CTG
Cuts at: 0 103 615 1164
Size: 103 512 549
Fragments arranged by size:
549 512 103
ApaI G_GGCC'C
Cuts at: 0 12 1164
Size: 12 1152
ApoI r'AATT_y
Cuts at: 0 1153 1164
Size: 1153 11
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 567 600 1164
Size: 567 33 564
Fragments arranged by size:
567 564 33
BamHI G'GATC_C
Cuts at: 0 263 1164
Size: 263 901
BanI G'GyrC_C
Cuts at: 0 202 473 859 1164
Size: 202 271 386 305
Fragments arranged by size:
386 305 271 202
BanII G_rGCy'C
Cuts at: 0 12 428 1164
Size: 12 416 736
Fragments arranged by size:
736 416 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 332 1164
Size: 332 832
BbvCI CC'TCA_GC
Cuts at: 0 104 1164
Size: 104 1060
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 916 950 1164
Size: 916 34 214
Fragments arranged by size:
916 214 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 855 1164
Size: 855 309
BclI T'GATC_A
Cuts at: 0 1007 1164
Size: 1007 157
BlpI GC'TnA_GC
Cuts at: 0 427 1164
Size: 427 737
Bme1580I G_kGCm'C
Cuts at: 0 12 1164
Size: 12 1152
BmgBI CAC'GTC
Cuts at: 0 731 1164
Size: 731 433
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 841 1164
Size: 261 580 323
Fragments arranged by size:
580 323 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 552 1164
Size: 307 245 612
Fragments arranged by size:
612 307 245
Bpu10I CC'TnA_GC
Cuts at: 0 104 1164
Size: 104 1060
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 1164
Size: 256 908
BsaAI yAC'GTr
Cuts at: 0 648 912 1164
Size: 648 264 252
Fragments arranged by size:
648 264 252
BsaHI Gr'CG_yC
Cuts at: 0 877 955 1164
Size: 877 78 209
Fragments arranged by size:
877 209 78
BsaWI w'CCGG_w
Cuts at: 0 622 1164
Size: 622 542
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 1164
Size: 253 911
BseYI C'CCAG_C
Cuts at: 0 174 1164
Size: 174 990
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 679 1164
Size: 121 558 485
Fragments arranged by size:
558 485 121
BsiEI CG_ry'CG
Cuts at: 0 50 1164
Size: 50 1114
BsiHKAI G_wGCw'C
Cuts at: 0 428 1042 1164
Size: 428 614 122
Fragments arranged by size:
614 428 122
BsmBI CGTCTCn'nnnn_
Cuts at: 0 814 1164
Size: 814 350
Bsp1286I G_dGCh'C
Cuts at: 0 12 428 1042 1164
Size: 12 416 614 122
Fragments arranged by size:
614 416 122 12
BspEI T'CCGG_A
Cuts at: 0 622 1164
Size: 622 542
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 136 1164
Size: 136 1028
BstAPI GCAn_nnn'nTGC
Cuts at: 0 648 1164
Size: 648 516
BstXI CCAn_nnnn'nTGG
Cuts at: 0 148 520 1086 1164
Size: 148 372 566 78
Fragments arranged by size:
566 372 148 78
BstYI r'GATC_y
Cuts at: 0 263 764 1164
Size: 263 501 400
Fragments arranged by size:
501 400 263
BtgI C'CryG_G
Cuts at: 0 50 442 671 777 812 848 1164
Size: 50 392 229 106 35 36 316
Fragments arranged by size:
392 316 229 106 50 36 35
EaeI y'GGCC_r
Cuts at: 0 25 47 891 1164
Size: 25 22 844 273
Fragments arranged by size:
844 273 25 22
EagI C'GGCC_G
Cuts at: 0 47 1164
Size: 47 1117
EarI CTCTTCn'nnn_
Cuts at: 0 196 1164
Size: 196 968
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 820 1164
Size: 820 344
EcoICRI GAG'CTC
Cuts at: 0 426 1164
Size: 426 738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 552 1164
Size: 307 245 612
Fragments arranged by size:
612 307 245
EcoRI G'AATT_C
Cuts at: 0 1153 1164
Size: 1153 11
HaeII r_GCGC'y
Cuts at: 0 18 616 907 994 1164
Size: 18 598 291 87 170
Fragments arranged by size:
598 291 170 87 18
HincII GTy'rAC
Cuts at: 0 728 1164
Size: 728 436
KpnI G_GTAC'C
Cuts at: 0 477 1164
Size: 477 687
MslI CAynn'nnrTG
Cuts at: 0 143 721 893 1015 1164
Size: 143 578 172 122 149
Fragments arranged by size:
578 172 149 143 122
MspA1I CmG'CkG
Cuts at: 0 30 52 431 444 770 779 850 1164
Size: 30 22 379 13 326 9 71 314
Fragments arranged by size:
379 326 314 71 30 22 13 9
NdeI CA'TA_TG
Cuts at: 0 1012 1164
Size: 1012 152
NotI GC'GGCC_GC
Cuts at: 0 47 1164
Size: 47 1117
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1164
Size: 699 465
PfoI T'CCnGG_A
Cuts at: 0 392 1164
Size: 392 772
PspOMI G'GGCC_C
Cuts at: 0 8 1164
Size: 8 1156
PvuII CAG'CTG
Cuts at: 0 431 1164
Size: 431 733
SacI G_AGCT'C
Cuts at: 0 428 1164
Size: 428 736
SacII CC_GC'GG
Cuts at: 0 53 445 780 851 1164
Size: 53 392 335 71 313
Fragments arranged by size:
392 335 313 71 53
ScaI AGT'ACT
Cuts at: 0 664 1164
Size: 664 500
SmlI C'TyrA_G
Cuts at: 0 235 1164
Size: 235 929
SnaBI TAC'GTA
Cuts at: 0 912 1164
Size: 912 252
SpeI A'CTAG_T
Cuts at: 0 360 1164
Size: 360 804
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 346 1164
Size: 346 818
TatI w'GTAC_w
Cuts at: 0 78 662 1164
Size: 78 584 502
Fragments arranged by size:
584 502 78
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1078 1164
Size: 1078 86
ZraI GAC'GTC
Cuts at: 0 956 1164
Size: 956 208
Enzymes that do cut:
AarI AatII AccI Acc65I AflIII AleI AlfI AlwNI
ApaI ApoI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BlpI Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BstAPI BstXI
BstYI BtgI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI HaeII HincII KpnI MslI MspA1I NdeI NotI
PflMI PfoI PspOMI PvuII SacI SacII ScaI SmlI
SnaBI SpeI TaqII TatI XcmI ZraI
Enzymes that do not cut:
AclI AcuI AfeI AflII AgeI AhdI AloI ApaLI
AscI AseI AsiSI AvaI AvrII BbeI BfrBI BglI
BglII BmrI BmtI BsaI BsaBI BsaXI BsiWI BsmI
BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EcoNI EcoO109I EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KasI MfeI MluI MmeI
MscI NaeI NarI NcoI NgoMIV NheI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SphI SrfI SspI StuI StyI SwaI
TstI Tth111I XbaI XhoI XmaI XmnI