Restriction map for
pF1KB7235
(Linear) (Six-base) MAPSORT of: KIBB7235.seq Check: 267 from: 1 to: 1905
KIBB7235.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
Acc65I G'GTAC_C
Cuts at: 0 980 1905
Size: 980 925
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 433 677 907 1905
Size: 133 300 244 230 998
Fragments arranged by size:
998 300 244 230 133
AflIII A'CryG_T
Cuts at: 0 272 1905
Size: 272 1633
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 981 1568 1905
Size: 981 587 337
Fragments arranged by size:
981 587 337
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1682 1714 1905
Size: 1682 32 191
Fragments arranged by size:
1682 191 32
AlwNI CAG_nnn'CTG
Cuts at: 0 195 610 1905
Size: 195 415 1295
Fragments arranged by size:
1295 415 195
ApaI G_GGCC'C
Cuts at: 0 15 1332 1905
Size: 15 1317 573
Fragments arranged by size:
1317 573 15
AvaI C'yCGr_G
Cuts at: 0 1113 1324 1331 1905
Size: 1113 211 7 574
Fragments arranged by size:
1113 574 211 7
AvrII C'CTAG_G
Cuts at: 0 1614 1905
Size: 1614 291
BamHI G'GATC_C
Cuts at: 0 808 1151 1905
Size: 808 343 754
Fragments arranged by size:
808 754 343
BanI G'GyrC_C
Cuts at: 0 980 1905
Size: 980 925
BanII G_rGCy'C
Cuts at: 0 15 110 1141 1332 1426 1651 1905
Size: 15 95 1031 191 94 225 254
Fragments arranged by size:
1031 254 225 191 95 94 15
BbvCI CC'TCA_GC
Cuts at: 0 1510 1537 1905
Size: 1510 27 368
Fragments arranged by size:
1510 368 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 257 291 1905
Size: 257 34 1614
Fragments arranged by size:
1614 257 34
BglII A'GATC_T
Cuts at: 0 1199 1905
Size: 1199 706
BlpI GC'TnA_GC
Cuts at: 0 635 1905
Size: 635 1270
Bme1580I G_kGCm'C
Cuts at: 0 15 960 1332 1822 1905
Size: 15 945 372 490 83
Fragments arranged by size:
945 490 372 83 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 170 401 1905
Size: 170 231 1504
Fragments arranged by size:
1504 231 170
BmtI G_CTAG'C
Cuts at: 0 929 1905
Size: 929 976
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 721 863 1188 1212 1333 1366 1440
Size: 458 263 142 325 24 121 33 74
Cuts at: 1440 1905
Size: 465
Fragments arranged by size:
465 458 325 263 142 121 74 33
24
Bpu10I CC'TnA_GC
Cuts at: 0 1510 1537 1645 1905
Size: 1510 27 108 260
Fragments arranged by size:
1510 260 108 27
BsaI GGTCTCn'nnnn_
Cuts at: 0 1252 1444 1456 1905
Size: 1252 192 12 449
Fragments arranged by size:
1252 449 192 12
BsaAI yAC'GTr
Cuts at: 0 273 1905
Size: 273 1632
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 836 866 1123 1153 1682 1712 1905
Size: 836 30 257 30 529 30 193
Fragments arranged by size:
836 529 257 193 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 478 1349 1905
Size: 28 450 871 556
Fragments arranged by size:
871 556 450 28
BseYI C'CCAG_C
Cuts at: 0 458 1503 1693 1905
Size: 458 1045 190 212
Fragments arranged by size:
1045 458 212 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1538 1588 1905
Size: 304 1234 50 317
Fragments arranged by size:
1234 317 304 50
BsiHKAI G_wGCw'C
Cuts at: 0 636 1426 1905
Size: 636 790 479
Fragments arranged by size:
790 636 479
BsmBI CGTCTCn'nnnn_
Cuts at: 0 310 1905
Size: 310 1595
Bsp1286I G_dGCh'C
Cuts at: 0 15 110 636 960 1141 1332 1426 1651
Size: 15 95 526 324 181 191 94 225
Cuts at: 1651 1822 1905
Size: 171 83
Fragments arranged by size:
526 324 225 191 181 171 95 94
83 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 583 845 1610 1905
Size: 583 262 765 295
Fragments arranged by size:
765 583 295 262
BsrBI CCG'CTC
Cuts at: 0 1489 1905
Size: 1489 416
BsrDI GCAATG_nn'
Cuts at: 0 874 1859 1905
Size: 874 985 46
Fragments arranged by size:
985 874 46
BsrFI r'CCGG_y
Cuts at: 0 396 1905
Size: 396 1509
BssHII G'CGCG_C
Cuts at: 0 1383 1905
Size: 1383 522
BssSI C'ACGA_G
Cuts at: 0 1425 1905
Size: 1425 480
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1500 1905
Size: 1500 405
BstEII G'GTnAC_C
Cuts at: 0 24 1101 1482 1905
Size: 24 1077 381 423
Fragments arranged by size:
1077 423 381 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1244 1905
Size: 1244 661
BstYI r'GATC_y
Cuts at: 0 522 785 808 1151 1199 1472 1905
Size: 522 263 23 343 48 273 433
Fragments arranged by size:
522 433 343 273 263 48 23
Bsu36I CC'TnA_GG
Cuts at: 0 493 1627 1905
Size: 493 1134 278
Fragments arranged by size:
1134 493 278
BtgI C'CryG_G
Cuts at: 0 208 422 545 1826 1905
Size: 208 214 123 1281 79
Fragments arranged by size:
1281 214 208 123 79
BtsI GCAGTG_nn'
Cuts at: 0 857 1634 1905
Size: 857 777 271
Fragments arranged by size:
857 777 271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1427 1462 1842 1877 1905
Size: 1427 35 380 35 28
Fragments arranged by size:
1427 380 35 35 28
EarI CTCTTCn'nnn_
Cuts at: 0 22 158 313 998 1004 1905
Size: 22 136 155 685 6 901
Fragments arranged by size:
901 685 155 136 22 6
EcoICRI GAG'CTC
Cuts at: 0 1424 1905
Size: 1424 481
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 433 458 677 721 863 907 1188
Size: 133 300 25 219 44 142 44 281
Cuts at: 1188 1212 1333 1366 1440 1905
Size: 24 121 33 74 465
Fragments arranged by size:
465 300 281 219 142 133 121 74
44 44 33 25 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1096 1725 1905
Size: 1096 629 180
Fragments arranged by size:
1096 629 180
EcoO109I rG'GnC_Cy
Cuts at: 0 12 138 1328 1340 1414 1611 1841 1905
Size: 12 126 1190 12 74 197 230 64
Fragments arranged by size:
1190 230 197 126 74 64 12 12
FspI TGC'GCA
Cuts at: 0 769 1905
Size: 769 1136
HaeII r_GCGC'y
Cuts at: 0 175 1517 1905
Size: 175 1342 388
Fragments arranged by size:
1342 388 175
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 345 377 772 804 1251 1283 1825 1857
Size: 345 32 395 32 447 32 542 32
Cuts at: 1857 1905
Size: 48
Fragments arranged by size:
542 447 395 345 48 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 689 1905
Size: 689 1216
KpnI G_GTAC'C
Cuts at: 0 984 1905
Size: 984 921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1412 1905
Size: 1412 493
MslI CAynn'nnrTG
Cuts at: 0 1093 1905
Size: 1093 812
MspA1I CmG'CkG
Cuts at: 0 192 210 318 458 558 765 1380 1905
Size: 192 18 108 140 100 207 615 525
Fragments arranged by size:
615 525 207 192 140 108 100 18
NaeI GCC'GGC
Cuts at: 0 398 1905
Size: 398 1507
NcoI C'CATG_G
Cuts at: 0 422 1826 1905
Size: 422 1404 79
Fragments arranged by size:
1404 422 79
NgoMIV G'CCGG_C
Cuts at: 0 396 1905
Size: 396 1509
NheI G'CTAG_C
Cuts at: 0 925 1905
Size: 925 980
NspI r_CATG'y
Cuts at: 0 252 1579 1905
Size: 252 1327 326
Fragments arranged by size:
1327 326 252
PfoI T'CCnGG_A
Cuts at: 0 1023 1905
Size: 1023 882
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1682 1714 1905
Size: 1682 32 191
Fragments arranged by size:
1682 191 32
PpuMI rG'GwC_Cy
Cuts at: 0 138 1340 1414 1611 1841 1905
Size: 138 1202 74 197 230 64
Fragments arranged by size:
1202 230 197 138 74 64
PspOMI G'GGCC_C
Cuts at: 0 11 1328 1905
Size: 11 1317 577
Fragments arranged by size:
1317 577 11
PstI C_TGCA'G
Cuts at: 0 593 764 1641 1905
Size: 593 171 877 264
Fragments arranged by size:
877 593 264 171
PvuII CAG'CTG
Cuts at: 0 192 458 558 765 1380 1905
Size: 192 266 100 207 615 525
Fragments arranged by size:
615 525 266 207 192 100
SacI G_AGCT'C
Cuts at: 0 1426 1905
Size: 1426 479
SacII CC_GC'GG
Cuts at: 0 211 1905
Size: 211 1694
SanDI GG'GwC_CC
Cuts at: 0 1340 1905
Size: 1340 565
SfcI C'TryA_G
Cuts at: 0 589 760 1030 1560 1637 1905
Size: 589 171 270 530 77 268
Fragments arranged by size:
589 530 270 268 171 77
SmaI CCC'GGG
Cuts at: 0 1115 1905
Size: 1115 790
SphI G_CATG'C
Cuts at: 0 252 1579 1905
Size: 252 1327 326
Fragments arranged by size:
1327 326 252
StuI AGG'CCT
Cuts at: 0 392 597 1905
Size: 392 205 1308
Fragments arranged by size:
1308 392 205
StyI C'CwwG_G
Cuts at: 0 422 878 933 1410 1478 1614 1655 1826
Size: 422 456 55 477 68 136 41 171
Cuts at: 1826 1905
Size: 79
Fragments arranged by size:
477 456 422 171 136 79 68 55
41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 318 415 1250 1839 1870 1905
Size: 318 97 835 589 31 35
Fragments arranged by size:
835 589 318 97 35 31
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 836 868 1210 1225 1242 1257 1905
Size: 836 32 342 15 17 15 648
Fragments arranged by size:
836 648 342 32 17 15 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1187 1212 1889 1905
Size: 1187 25 677 16
Fragments arranged by size:
1187 677 25 16
XmaI C'CCGG_G
Cuts at: 0 1113 1905
Size: 1113 792
XmnI GAAnn'nnTTC
Cuts at: 0 118 340 1905
Size: 118 222 1565
Fragments arranged by size:
1565 222 118
Enzymes that do cut:
Acc65I AcuI AflIII AlfI AloI AlwNI ApaI AvaI
AvrII BamHI BanI BanII BbvCI BcgI BglII BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI CspCI EarI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HindIII KpnI MmeI MslI
MspA1I NaeI NcoI NgoMIV NheI NspI PfoI PpiI
PpuMI PspOMI PstI PvuII SacI SacII SanDI SfcI
SmaI SphI StuI StyI TaqII TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI ApaLI ApoI AscI AseI AsiSI BaeI BbeI
BbsI BciVI BclI BfrBI BglI BmgBI BplI BpuEI
BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI
BsrGI BstBI BstZ17I BtgZI ClaI DraI DraIII DrdI
EaeI EagI EciI EcoRI EcoRV FalI FseI FspAI
HincII HpaI KasI MfeI MluI MscI NarI NdeI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SrfI SspI SwaI TatI Tth111I
XbaI XhoI ZraI