Restriction map for
pF1KB7244
(Linear) (Six-base) MAPSORT of: KIBB7244.seq Check: 2703 from: 1 to: 1092
KIBB7244.orf
With 182 enzymes: SgfI *
October 7, 2009 14:23 ..
AatII G_ACGT'C
Cuts at: 0 260 1092
Size: 260 832
Acc65I G'GTAC_C
Cuts at: 0 550 1092
Size: 550 542
AleI CACnn'nnGTG
Cuts at: 0 616 1092
Size: 616 476
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 529 1092
Size: 529 563
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 28 60 289 321 1092
Size: 27 1 32 229 32 771
Fragments arranged by size:
771 229 32 32 27 1
ApaI G_GGCC'C
Cuts at: 0 114 1092
Size: 114 978
ApaLI G'TGCA_C
Cuts at: 0 424 1092
Size: 424 668
AvaI C'yCGr_G
Cuts at: 0 48 113 1092
Size: 48 65 979
Fragments arranged by size:
979 65 48
BamHI G'GATC_C
Cuts at: 0 1016 1092
Size: 1016 76
BanI G'GyrC_C
Cuts at: 0 96 126 449 550 592 976 1092
Size: 96 30 323 101 42 384 116
Fragments arranged by size:
384 323 116 101 96 42 30
BanII G_rGCy'C
Cuts at: 0 8 114 439 578 839 973 1092
Size: 8 106 325 139 261 134 119
Fragments arranged by size:
325 261 139 134 119 106 8
BbeI G_GCGC'C
Cuts at: 0 100 453 596 1092
Size: 100 353 143 496
Fragments arranged by size:
496 353 143 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 220 372 591 1092
Size: 220 152 219 501
Fragments arranged by size:
501 220 219 152
BglI GCCn_nnn'nGGC
Cuts at: 0 472 582 1092
Size: 472 110 510
Fragments arranged by size:
510 472 110
BlpI GC'TnA_GC
Cuts at: 0 433 1092
Size: 433 659
Bme1580I G_kGCm'C
Cuts at: 0 114 131 428 1092
Size: 114 17 297 664
Fragments arranged by size:
664 297 114 17
BmgBI CAC'GTC
Cuts at: 0 537 1092
Size: 537 555
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 858 1092
Size: 858 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1092
Size: 83 1009
BsaAI yAC'GTr
Cuts at: 0 189 1092
Size: 189 903
BsaHI Gr'CG_yC
Cuts at: 0 97 257 359 450 593 992 1092
Size: 97 160 102 91 143 399 100
Fragments arranged by size:
399 160 143 102 100 97 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 28 58 1092
Size: 25 3 30 1034
Fragments arranged by size:
1034 30 25 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 1092
Size: 33 1059
BseYI C'CCAG_C
Cuts at: 0 15 28 162 270 621 1092
Size: 15 13 134 108 351 471
Fragments arranged by size:
471 351 134 108 15 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 1092
Size: 559 533
BsiEI CG_ry'CG
Cuts at: 0 158 617 1092
Size: 158 459 475
Fragments arranged by size:
475 459 158
BsiHKAI G_wGCw'C
Cuts at: 0 428 439 1092
Size: 428 11 653
Fragments arranged by size:
653 428 11
Bsp1286I G_dGCh'C
Cuts at: 0 8 114 131 428 439 578 839 973
Size: 8 106 17 297 11 139 261 134
Cuts at: 973 1092
Size: 119
Fragments arranged by size:
297 261 139 134 119 106 17 11
8
BspHI T'CATG_A
Cuts at: 0 344 395 1092
Size: 344 51 697
Fragments arranged by size:
697 344 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 562 671 1092
Size: 149 413 109 421
Fragments arranged by size:
421 413 149 109
BsrBI CCG'CTC
Cuts at: 0 519 729 1092
Size: 519 210 363
Fragments arranged by size:
519 363 210
BsrFI r'CCGG_y
Cuts at: 0 93 847 1092
Size: 93 754 245
Fragments arranged by size:
754 245 93
BsrGI T'GTAC_A
Cuts at: 0 263 1092
Size: 263 829
BstYI r'GATC_y
Cuts at: 0 1016 1092
Size: 1016 76
BtgI C'CryG_G
Cuts at: 0 482 578 818 995 1092
Size: 482 96 240 177 97
Fragments arranged by size:
482 240 177 97 96
BtsI GCAGTG_nn'
Cuts at: 0 254 425 1092
Size: 254 171 667
Fragments arranged by size:
667 254 171
EaeI y'GGCC_r
Cuts at: 0 155 251 287 464 568 614 815 1092
Size: 155 96 36 177 104 46 201 277
Fragments arranged by size:
277 201 177 155 104 96 46 36
EagI C'GGCC_G
Cuts at: 0 155 614 1092
Size: 155 459 478
Fragments arranged by size:
478 459 155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 834 922 1092
Size: 834 88 170
Fragments arranged by size:
834 170 88
EcoICRI GAG'CTC
Cuts at: 0 437 1092
Size: 437 655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 1092
Size: 83 1009
EcoNI CCTnn'n_nnAGG
Cuts at: 0 964 1092
Size: 964 128
EcoO109I rG'GnC_Cy
Cuts at: 0 110 492 1092
Size: 110 382 600
Fragments arranged by size:
600 382 110
HaeII r_GCGC'y
Cuts at: 0 100 453 482 596 723 826 1092
Size: 100 353 29 114 127 103 266
Fragments arranged by size:
353 266 127 114 103 100 29
HincII GTy'rAC
Cuts at: 0 363 801 810 1092
Size: 363 438 9 282
Fragments arranged by size:
438 363 282 9
KasI G'GCGC_C
Cuts at: 0 96 449 592 1092
Size: 96 353 143 500
Fragments arranged by size:
500 353 143 96
KpnI G_GTAC'C
Cuts at: 0 554 1092
Size: 554 538
MscI TGG'CCA
Cuts at: 0 289 466 1092
Size: 289 177 626
Fragments arranged by size:
626 289 177
MslI CAynn'nnrTG
Cuts at: 0 187 400 616 673 1092
Size: 187 213 216 57 419
Fragments arranged by size:
419 216 213 187 57
MspA1I CmG'CkG
Cuts at: 0 32 432 446 484 566 639 890 1092
Size: 32 400 14 38 82 73 251 202
Fragments arranged by size:
400 251 202 82 73 38 32 14
NaeI GCC'GGC
Cuts at: 0 95 849 1092
Size: 95 754 243
Fragments arranged by size:
754 243 95
NarI GG'CG_CC
Cuts at: 0 97 450 593 1092
Size: 97 353 143 499
Fragments arranged by size:
499 353 143 97
NgoMIV G'CCGG_C
Cuts at: 0 93 847 1092
Size: 93 754 245
Fragments arranged by size:
754 245 93
NspI r_CATG'y
Cuts at: 0 270 510 1092
Size: 270 240 582
Fragments arranged by size:
582 270 240
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 28 60 1092
Size: 27 1 32 1032
Fragments arranged by size:
1032 32 27 1
PpuMI rG'GwC_Cy
Cuts at: 0 492 1092
Size: 492 600
PspOMI G'GGCC_C
Cuts at: 0 110 1092
Size: 110 982
PvuII CAG'CTG
Cuts at: 0 32 566 890 1092
Size: 32 534 324 202
Fragments arranged by size:
534 324 202 32
SacI G_AGCT'C
Cuts at: 0 439 1092
Size: 439 653
SacII CC_GC'GG
Cuts at: 0 485 1092
Size: 485 607
SfoI GGC'GCC
Cuts at: 0 98 451 594 1092
Size: 98 353 143 498
Fragments arranged by size:
498 353 143 98
SmaI CCC'GGG
Cuts at: 0 50 115 1092
Size: 50 65 977
Fragments arranged by size:
977 65 50
SrfI GCCC'GGGC
Cuts at: 0 115 1092
Size: 115 977
StyI C'CwwG_G
Cuts at: 0 980 1092
Size: 980 112
TatI w'GTAC_w
Cuts at: 0 263 1092
Size: 263 829
Tth111I GACn'n_nGTC
Cuts at: 0 358 1092
Size: 358 734
XmaI C'CCGG_G
Cuts at: 0 48 113 1092
Size: 48 65 979
Fragments arranged by size:
979 65 48
ZraI GAC'GTC
Cuts at: 0 258 1092
Size: 258 834
Enzymes that do cut:
AatII Acc65I AleI AlfI AloI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BglI BlpI Bme1580I
BmgBI BplI BpmI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BstYI BtgI BtsI EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII HincII KasI KpnI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PpiI PpuMI
PspOMI PvuII SacI SacII SfoI SmaI SrfI StyI
TatI Tth111I XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AlwNI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI
Bpu10I BpuEI BsaI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EarI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI MfeI MluI MmeI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI
StuI SwaI TaqII TstI XbaI XcmI XhoI XmnI