(Linear) (Six-base) MAPSORT of: KIBB7253.seq  Check: 1935  from: 1  to: 1257



KIBB7253.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     896    1257 
   Size:        896     361
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     963    1257 
   Size:        963     294
 
ApoI r'AATT_y

Cuts at:      0     546     557     630     753    1031    1257 
   Size:        546      11      73     123     278     226

  Fragments arranged by size: 

                 546     278     226     123      73      11
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     335     998    1257 
   Size:        335     663     259

  Fragments arranged by size: 

                 663     335     259
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1031    1257 
   Size:       1031     226
 
BclI T'GATC_A

Cuts at:      0    1056    1257 
   Size:       1056     201
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1009    1257 
   Size:       1009     248
 
BmtI G_CTAG'C

Cuts at:      0     578    1257 
   Size:        578     679
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2    1257 
   Size:          2    1255
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710    1257 
   Size:        710     547
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     933     956    1257 
   Size:        933      23     301

  Fragments arranged by size: 

                 933     301      23
 
BsmI GAATG_Cn'

Cuts at:      0     320     576    1257 
   Size:        320     256     681

  Fragments arranged by size: 

                 681     320     256
 
BsrGI T'GTAC_A

Cuts at:      0      87    1036    1257 
   Size:         87     949     221

  Fragments arranged by size: 

                 949     221      87
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     943    1257 
   Size:        943     314
 
BstYI r'GATC_y

Cuts at:      0    1106    1257 
   Size:       1106     151
 
BtsI GCAGTG_nn'

Cuts at:      0    1247    1254    1257 
   Size:       1247       7       3

  Fragments arranged by size: 

                1247       7       3
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     758     793    1257 
   Size:        758      35     464

  Fragments arranged by size: 

                 758     464      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1198    1257 
   Size:       1198      59
 
EaeI y'GGCC_r

Cuts at:      0     996    1257 
   Size:        996     261
 
EarI CTCTTCn'nnn_

Cuts at:      0     983    1257 
   Size:        983     274
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       2     896    1257 
   Size:          2     894     361

  Fragments arranged by size: 

                 894     361       2
 
EcoO109I rG'GnC_Cy

Cuts at:      0     136    1257 
   Size:        136    1121
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     359    1257 
   Size:        359     898
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     586     618    1257 
   Size:        586      32     639

  Fragments arranged by size: 

                 639     586      32
 
HincII GTy'rAC

Cuts at:      0     507    1257 
   Size:        507     750
 
MscI TGG'CCA

Cuts at:      0     998    1257 
   Size:        998     259
 
MslI CAynn'nnrTG

Cuts at:      0      50    1257 
   Size:         50    1207
 
NheI G'CTAG_C

Cuts at:      0     574    1257 
   Size:        574     683
 
NspI r_CATG'y

Cuts at:      0     513    1257 
   Size:        513     744
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     198     271     355    1257 
   Size:        198      73      84     902

  Fragments arranged by size: 

                 902     198      84      73
 
PfoI T'CCnGG_A

Cuts at:      0     615    1257 
   Size:        615     642
 
PpuMI rG'GwC_Cy

Cuts at:      0     136    1257 
   Size:        136    1121
 
PsiI TTA'TAA

Cuts at:      0     482    1257 
   Size:        482     775
 
PstI C_TGCA'G

Cuts at:      0    1152    1164    1242    1257 
   Size:       1152      12      78      15

  Fragments arranged by size: 

                1152      78      15      12
 
SexAI A'CCwGG_T

Cuts at:      0      81    1257 
   Size:         81    1176
 
SfcI C'TryA_G

Cuts at:      0     300    1148    1160    1238    1257 
   Size:        300     848      12      78      19

  Fragments arranged by size: 

                 848     300      78      19      12
 
SmlI C'TyrA_G

Cuts at:      0     725    1257 
   Size:        725     532
 
SspI AAT'ATT

Cuts at:      0     810    1257 
   Size:        810     447
 
StuI AGG'CCT

Cuts at:      0    1081    1257 
   Size:       1081     176
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     401     998    1257 
   Size:        401     597     259

  Fragments arranged by size: 

                 597     401     259
 
TatI w'GTAC_w

Cuts at:      0      87     304    1036    1111    1257 
   Size:         87     217     732      75     146

  Fragments arranged by size: 

                 732     217     146      87      75
 
XmnI GAAnn'nnTTC

Cuts at:      0     342     624    1257 
   Size:        342     282     633

  Fragments arranged by size: 

                 633     342     282
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BbsI    BciVI     BclI     BmrI     BmtI
     BpmI    BpuEI     BsgI     BsmI    BsrGI   BstAPI    BstYI     BtsI
    CspCI     DrdI     EaeI     EarI  Eco57MI EcoO109I     FalI    Hin4I
   HincII     MscI     MslI     NheI     NspI    PflMI     PfoI    PpuMI
     PsiI     PstI    SexAI     SfcI     SmlI     SspI     StuI    TaqII
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI