Restriction map for
pF1KB7254
(Linear) (Six-base) MAPSORT of: KIBB7254.seq Check: 1354 from: 1 to: 1932
KIBB7254.orf
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 195 1932
Size: 195 1737
AatII G_ACGT'C
Cuts at: 0 1529 1932
Size: 1529 403
AccI GT'mk_AC
Cuts at: 0 935 1932
Size: 935 997
AflII C'TTAA_G
Cuts at: 0 892 1932
Size: 892 1040
AflIII A'CryG_T
Cuts at: 0 356 1646 1892 1932
Size: 356 1290 246 40
Fragments arranged by size:
1290 356 246 40
AleI CACnn'nnGTG
Cuts at: 0 434 547 1903 1932
Size: 434 113 1356 29
Fragments arranged by size:
1356 434 113 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1408 1440 1932
Size: 1408 32 492
Fragments arranged by size:
1408 492 32
AlwNI CAG_nnn'CTG
Cuts at: 0 869 1695 1932
Size: 869 826 237
Fragments arranged by size:
869 826 237
ApaI G_GGCC'C
Cuts at: 0 1160 1932
Size: 1160 772
ApoI r'AATT_y
Cuts at: 0 280 903 1932
Size: 280 623 1029
Fragments arranged by size:
1029 623 280
AvaI C'yCGr_G
Cuts at: 0 1323 1932
Size: 1323 609
BamHI G'GATC_C
Cuts at: 0 1711 1932
Size: 1711 221
BanI G'GyrC_C
Cuts at: 0 541 632 811 1838 1932
Size: 541 91 179 1027 94
Fragments arranged by size:
1027 541 179 94 91
BanII G_rGCy'C
Cuts at: 0 556 599 1160 1686 1932
Size: 556 43 561 526 246
Fragments arranged by size:
561 556 526 246 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 176 321 504 1932
Size: 176 145 183 1428
Fragments arranged by size:
1428 183 176 145
BbvCI CC'TCA_GC
Cuts at: 0 640 1214 1932
Size: 640 574 718
Fragments arranged by size:
718 640 574
BclI T'GATC_A
Cuts at: 0 926 1052 1932
Size: 926 126 880
Fragments arranged by size:
926 880 126
BglI GCCn_nnn'nGGC
Cuts at: 0 97 1932
Size: 97 1835
BglII A'GATC_T
Cuts at: 0 413 1932
Size: 413 1519
BlpI GC'TnA_GC
Cuts at: 0 70 555 1932
Size: 70 485 1377
Fragments arranged by size:
1377 485 70
Bme1580I G_kGCm'C
Cuts at: 0 1160 1170 1932
Size: 1160 10 762
Fragments arranged by size:
1160 762 10
Bpu10I CC'TnA_GC
Cuts at: 0 640 1214 1932
Size: 640 574 718
Fragments arranged by size:
718 640 574
BsaI GGTCTCn'nnnn_
Cuts at: 0 1678 1932
Size: 1678 254
BsaHI Gr'CG_yC
Cuts at: 0 1526 1793 1932
Size: 1526 267 139
Fragments arranged by size:
1526 267 139
BsaWI w'CCGG_w
Cuts at: 0 242 1932
Size: 242 1690
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1408 1438 1932
Size: 1408 30 494
Fragments arranged by size:
1408 494 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 813 1279 1288 1834 1932
Size: 813 466 9 546 98
Fragments arranged by size:
813 546 466 98 9
BseYI C'CCAG_C
Cuts at: 0 448 604 1159 1412 1555 1827 1932
Size: 448 156 555 253 143 272 105
Fragments arranged by size:
555 448 272 253 156 143 105
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 412 1932
Size: 243 169 1520
Fragments arranged by size:
1520 243 169
BsiEI CG_ry'CG
Cuts at: 0 7 1932
Size: 7 1925
BsiHKAI G_wGCw'C
Cuts at: 0 344 1932
Size: 344 1588
Bsp1286I G_dGCh'C
Cuts at: 0 344 556 599 1160 1170 1686 1932
Size: 344 212 43 561 10 516 246
Fragments arranged by size:
561 516 344 246 212 43 10
BspEI T'CCGG_A
Cuts at: 0 242 1932
Size: 242 1690
BspHI T'CATG_A
Cuts at: 0 761 995 1877 1932
Size: 761 234 882 55
Fragments arranged by size:
882 761 234 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 195 600 1919 1932
Size: 195 405 1319 13
Fragments arranged by size:
1319 405 195 13
BssHII G'CGCG_C
Cuts at: 0 26 1932
Size: 26 1906
BstAPI GCAn_nnn'nTGC
Cuts at: 0 257 869 1932
Size: 257 612 1063
Fragments arranged by size:
1063 612 257
BstBI TT'CG_AA
Cuts at: 0 990 1932
Size: 990 942
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 1932
Size: 462 1470
BstYI r'GATC_y
Cuts at: 0 413 1711 1932
Size: 413 1298 221
Fragments arranged by size:
1298 413 221
BtgI C'CryG_G
Cuts at: 0 1034 1139 1932
Size: 1034 105 793
Fragments arranged by size:
1034 793 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 542 1932
Size: 542 1390
BtsI GCAGTG_nn'
Cuts at: 0 1170 1932
Size: 1170 762
DraIII CAC_nnn'GTG
Cuts at: 0 1140 1454 1932
Size: 1140 314 478
Fragments arranged by size:
1140 478 314
DrdI GACnn_nn'nnGTC
Cuts at: 0 394 1932
Size: 394 1538
EaeI y'GGCC_r
Cuts at: 0 4 371 836 1037 1112 1360 1580 1595
Size: 4 367 465 201 75 248 220 15
Cuts at: 1595 1932
Size: 337
Fragments arranged by size:
465 367 337 248 220 201 75 15
4
EagI C'GGCC_G
Cuts at: 0 4 1932
Size: 4 1928
EarI CTCTTCn'nnn_
Cuts at: 0 1265 1932
Size: 1265 667
EcoO109I rG'GnC_Cy
Cuts at: 0 60 1156 1932
Size: 60 1096 776
Fragments arranged by size:
1096 776 60
EcoRI G'AATT_C
Cuts at: 0 280 1932
Size: 280 1652
HaeII r_GCGC'y
Cuts at: 0 103 426 1932
Size: 103 323 1506
Fragments arranged by size:
1506 323 103
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 931 963 1306 1338 1696 1703 1728 1735
Size: 931 32 343 32 358 7 25 7
Cuts at: 1735 1932
Size: 197
Fragments arranged by size:
931 358 343 197 32 32 25 7
7
HincII GTy'rAC
Cuts at: 0 828 1932
Size: 828 1104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1652 1932
Size: 1652 280
MscI TGG'CCA
Cuts at: 0 373 838 1039 1114 1582 1597 1932
Size: 373 465 201 75 468 15 335
Fragments arranged by size:
468 465 373 335 201 75 15
MslI CAynn'nnrTG
Cuts at: 0 355 434 460 547 1480 1621 1903 1932
Size: 355 79 26 87 933 141 282 29
Fragments arranged by size:
933 355 282 141 87 79 29 26
MspA1I CmG'CkG
Cuts at: 0 469 559 1365 1815 1932
Size: 469 90 806 450 117
Fragments arranged by size:
806 469 450 117 90
NcoI C'CATG_G
Cuts at: 0 1034 1932
Size: 1034 898
NspI r_CATG'y
Cuts at: 0 360 1050 1932
Size: 360 690 882
Fragments arranged by size:
882 690 360
PasI CC'CwG_GG
Cuts at: 0 1512 1679 1932
Size: 1512 167 253
Fragments arranged by size:
1512 253 167
PciI A'CATG_T
Cuts at: 0 356 1932
Size: 356 1576
PflMI CCAn_nnn'nTGG
Cuts at: 0 1454 1932
Size: 1454 478
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1408 1440 1932
Size: 1408 32 492
Fragments arranged by size:
1408 492 32
PpuMI rG'GwC_Cy
Cuts at: 0 60 1932
Size: 60 1872
PspOMI G'GGCC_C
Cuts at: 0 1156 1932
Size: 1156 776
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1843 1875 1932
Size: 1843 32 57
Fragments arranged by size:
1843 57 32
PstI C_TGCA'G
Cuts at: 0 1691 1932
Size: 1691 241
PvuII CAG'CTG
Cuts at: 0 1815 1932
Size: 1815 117
SfcI C'TryA_G
Cuts at: 0 1687 1932
Size: 1687 245
SmlI C'TyrA_G
Cuts at: 0 892 1932
Size: 892 1040
StyI C'CwwG_G
Cuts at: 0 404 1034 1932
Size: 404 630 898
Fragments arranged by size:
898 630 404
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 489 1660 1826 1932
Size: 489 1171 166 106
Fragments arranged by size:
1171 489 166 106
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 795 827 952 984 1932
Size: 795 32 125 32 948
Fragments arranged by size:
948 795 125 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 528 1590 1605 1932
Size: 528 1062 15 327
Fragments arranged by size:
1062 528 327 15
XmnI GAAnn'nnTTC
Cuts at: 0 1423 1932
Size: 1423 509
ZraI GAC'GTC
Cuts at: 0 1527 1932
Size: 1527 405
Enzymes that do cut:
AarI AatII AccI AflII AflIII AleI AloI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BclI BglI BglII BlpI Bme1580I Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BssHII BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
EcoO109I EcoRI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NcoI NspI PasI PciI PflMI PpiI PpuMI
PspOMI PsrI PstI PvuII SfcI SmlI StyI TaqII
TstI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AgeI AhdI AlfI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BfrBI BmgBI BmrI BmtI BplI BpmI BpuEI BsaAI
BsaBI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI
BssSI BstEII BstZ17I Bsu36I ClaI CspCI DraI EciI
EcoICRI Eco57MI EcoNI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PshAI PsiI PspXI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI Tth111I XbaI XhoI XmaI