Restriction map for
pF1KB7259
(Linear) (Six-base) MAPSORT of: KIBB7259.seq Check: 9966 from: 1 to: 1758
KIBB7259.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 306 1758
Size: 306 1452
AccI GT'mk_AC
Cuts at: 0 594 1673 1758
Size: 594 1079 85
Fragments arranged by size:
1079 594 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1758
Size: 1139 619
AfeI AGC'GCT
Cuts at: 0 67 85 1758
Size: 67 18 1673
Fragments arranged by size:
1673 67 18
AflII C'TTAA_G
Cuts at: 0 837 1086 1758
Size: 837 249 672
Fragments arranged by size:
837 672 249
AhdI GACnn_n'nnGTC
Cuts at: 0 807 906 1758
Size: 807 99 852
Fragments arranged by size:
852 807 99
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 139 171 1758
Size: 139 32 1587
Fragments arranged by size:
1587 139 32
ApaI G_GGCC'C
Cuts at: 0 217 1758
Size: 217 1541
ApaLI G'TGCA_C
Cuts at: 0 21 325 1758
Size: 21 304 1433
Fragments arranged by size:
1433 304 21
ApoI r'AATT_y
Cuts at: 0 235 1116 1582 1758
Size: 235 881 466 176
Fragments arranged by size:
881 466 235 176
AseI AT'TA_AT
Cuts at: 0 1463 1758
Size: 1463 295
AvaI C'yCGr_G
Cuts at: 0 49 747 1758
Size: 49 698 1011
Fragments arranged by size:
1011 698 49
BanI G'GyrC_C
Cuts at: 0 738 959 1201 1561 1758
Size: 738 221 242 360 197
Fragments arranged by size:
738 360 242 221 197
BanII G_rGCy'C
Cuts at: 0 217 732 1192 1758
Size: 217 515 460 566
Fragments arranged by size:
566 515 460 217
BbeI G_GCGC'C
Cuts at: 0 963 1758
Size: 963 795
BbsI GAAGACnn'nnnn_
Cuts at: 0 582 803 1175 1508 1705 1758
Size: 582 221 372 333 197 53
Fragments arranged by size:
582 372 333 221 197 53
BbvCI CC'TCA_GC
Cuts at: 0 184 1758
Size: 184 1574
BfrBI ATG'CAT
Cuts at: 0 1393 1758
Size: 1393 365
BglII A'GATC_T
Cuts at: 0 1320 1758
Size: 1320 438
Bme1580I G_kGCm'C
Cuts at: 0 25 217 329 1184 1566 1758
Size: 25 192 112 855 382 192
Fragments arranged by size:
855 382 192 192 112 25
BmrI ACTGGGnnnn_n'
Cuts at: 0 1303 1758
Size: 1303 455
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 408 1758
Size: 408 1350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 382 675 792 1430 1696 1758
Size: 141 241 293 117 638 266 62
Fragments arranged by size:
638 293 266 241 141 117 62
Bpu10I CC'TnA_GC
Cuts at: 0 184 731 935 1280 1552 1758
Size: 184 547 204 345 272 206
Fragments arranged by size:
547 345 272 206 204 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1251 1309 1758
Size: 1251 58 449
Fragments arranged by size:
1251 449 58
BsaAI yAC'GTr
Cuts at: 0 1571 1758
Size: 1571 187
BsaBI GATnn'nnATC
Cuts at: 0 863 1758
Size: 863 895
BsaHI Gr'CG_yC
Cuts at: 0 960 1758
Size: 960 798
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 388 418 906 936 1694 1724
Size: 139 30 219 30 488 30 758 30
Cuts at: 1724 1758
Size: 34
Fragments arranged by size:
758 488 219 139 34 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 396 450 982 1343 1543 1758
Size: 396 54 532 361 200 215
Fragments arranged by size:
532 396 361 215 200 54
BseYI C'CCAG_C
Cuts at: 0 217 1758
Size: 217 1541
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 392 565 1758
Size: 215 177 173 1193
Fragments arranged by size:
1193 215 177 173
BsiHKAI G_wGCw'C
Cuts at: 0 25 329 732 1535 1720 1758
Size: 25 304 403 803 185 38
Fragments arranged by size:
803 403 304 185 38 25
BsmI GAATG_Cn'
Cuts at: 0 1009 1758
Size: 1009 749
Bsp1286I G_dGCh'C
Cuts at: 0 25 217 329 732 1184 1192 1535 1566
Size: 25 192 112 403 452 8 343 31
Cuts at: 1566 1720 1758
Size: 154 38
Fragments arranged by size:
452 403 343 192 154 112 38 31
25 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 306 1118 1758
Size: 306 812 640
Fragments arranged by size:
812 640 306
BsrBI CCG'CTC
Cuts at: 0 116 684 1758
Size: 116 568 1074
Fragments arranged by size:
1074 568 116
BsrDI GCAATG_nn'
Cuts at: 0 820 1758
Size: 820 938
BsrFI r'CCGG_y
Cuts at: 0 25 1758
Size: 25 1733
BstAPI GCAn_nnn'nTGC
Cuts at: 0 312 1758
Size: 312 1446
BstXI CCAn_nnnn'nTGG
Cuts at: 0 699 724 1758
Size: 699 25 1034
Fragments arranged by size:
1034 699 25
BstYI r'GATC_y
Cuts at: 0 1320 1758
Size: 1320 438
Bsu36I CC'TnA_GG
Cuts at: 0 755 1758
Size: 755 1003
BtgI C'CryG_G
Cuts at: 0 380 717 1758
Size: 380 337 1041
Fragments arranged by size:
1041 380 337
BtsI GCAGTG_nn'
Cuts at: 0 23 203 729 1221 1563 1758
Size: 23 180 526 492 342 195
Fragments arranged by size:
526 492 342 195 180 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 952 987 1758
Size: 952 35 771
Fragments arranged by size:
952 771 35
DraIII CAC_nnn'GTG
Cuts at: 0 985 1758
Size: 985 773
EaeI y'GGCC_r
Cuts at: 0 293 354 1345 1758
Size: 293 61 991 413
Fragments arranged by size:
991 413 293 61
EarI CTCTTCn'nnn_
Cuts at: 0 538 1758
Size: 538 1220
EcoICRI GAG'CTC
Cuts at: 0 730 1758
Size: 730 1028
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 382 675 792 1139 1430 1696 1758
Size: 141 241 293 117 347 291 266 62
Fragments arranged by size:
347 293 291 266 241 141 117 62
EcoO109I rG'GnC_Cy
Cuts at: 0 214 1406 1758
Size: 214 1192 352
Fragments arranged by size:
1192 352 214
HaeII r_GCGC'y
Cuts at: 0 69 87 963 1758
Size: 69 18 876 795
Fragments arranged by size:
876 795 69 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 601 633 727 759 793 825 892 924
Size: 601 32 94 32 34 32 67 32
Cuts at: 924 1692 1724 1758
Size: 768 32 34
Fragments arranged by size:
768 601 94 67 34 34 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 669 1758
Size: 669 1089
KasI G'GCGC_C
Cuts at: 0 959 1758
Size: 959 799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 584 935 1758
Size: 584 351 823
Fragments arranged by size:
823 584 351
MscI TGG'CCA
Cuts at: 0 295 356 1758
Size: 295 61 1402
Fragments arranged by size:
1402 295 61
MslI CAynn'nnrTG
Cuts at: 0 172 722 1246 1522 1758
Size: 172 550 524 276 236
Fragments arranged by size:
550 524 276 236 172
NarI GG'CG_CC
Cuts at: 0 960 1758
Size: 960 798
NcoI C'CATG_G
Cuts at: 0 380 717 1758
Size: 380 337 1041
Fragments arranged by size:
1041 380 337
NsiI A_TGCA'T
Cuts at: 0 1395 1758
Size: 1395 363
NspI r_CATG'y
Cuts at: 0 211 1393 1758
Size: 211 1182 365
Fragments arranged by size:
1182 365 211
PasI CC'CwG_GG
Cuts at: 0 797 1758
Size: 797 961
PfoI T'CCnGG_A
Cuts at: 0 1382 1758
Size: 1382 376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 1758
Size: 139 32 1587
Fragments arranged by size:
1587 139 32
PpuMI rG'GwC_Cy
Cuts at: 0 1406 1758
Size: 1406 352
PspOMI G'GGCC_C
Cuts at: 0 213 1758
Size: 213 1545
PspXI vC'TCGA_Gb
Cuts at: 0 747 1758
Size: 747 1011
PstI C_TGCA'G
Cuts at: 0 416 680 1228 1758
Size: 416 264 548 530
Fragments arranged by size:
548 530 416 264
SacI G_AGCT'C
Cuts at: 0 732 1758
Size: 732 1026
ScaI AGT'ACT
Cuts at: 0 139 1758
Size: 139 1619
SexAI A'CCwGG_T
Cuts at: 0 287 1758
Size: 287 1471
SfcI C'TryA_G
Cuts at: 0 61 141 412 676 1224 1758
Size: 61 80 271 264 548 534
Fragments arranged by size:
548 534 271 264 80 61
SfoI GGC'GCC
Cuts at: 0 961 1758
Size: 961 797
SmlI C'TyrA_G
Cuts at: 0 747 837 1086 1266 1324 1758
Size: 747 90 249 180 58 434
Fragments arranged by size:
747 434 249 180 90 58
StuI AGG'CCT
Cuts at: 0 753 1758
Size: 753 1005
StyI C'CwwG_G
Cuts at: 0 351 380 717 1758
Size: 351 29 337 1041
Fragments arranged by size:
1041 351 337 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 810 1758
Size: 312 498 948
Fragments arranged by size:
948 498 312
TatI w'GTAC_w
Cuts at: 0 137 976 1758
Size: 137 839 782
Fragments arranged by size:
839 782 137
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 906 938 999 1031 1694 1726 1758
Size: 906 32 61 32 663 32 32
Fragments arranged by size:
906 663 61 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1678 1758
Size: 1678 80
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 364 1758
Size: 364 1394
XhoI C'TCGA_G
Cuts at: 0 747 1758
Size: 747 1011
XmnI GAAnn'nnTTC
Cuts at: 0 247 1758
Size: 247 1511
Enzymes that do cut:
AarI AccI AcuI AfeI AflII AhdI AloI ApaI
ApaLI ApoI AseI AvaI BanI BanII BbeI BbsI
BbvCI BfrBI BglII Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI
BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI
EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI
MmeI MscI MslI NarI NcoI NsiI NspI PasI
PfoI PpiI PpuMI PspOMI PspXI PstI SacI ScaI
SexAI SfcI SfoI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflIII AgeI AleI AlfI AlwNI
AscI AsiSI AvrII BaeI BamHI BcgI BciVI BclI
BglI BlpI BmgBI BmtI BsaI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI DraI DrdI EagI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MspA1I NaeI NdeI NgoMIV NheI
NotI NruI PacI PciI PflMI PmeI PmlI PshAI
PsiI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmaI ZraI