(Linear) (Six-base) MAPSORT of: KIBB7261.seq Check: 3653 from: 1 to: 588
KIBB7261.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 329 588
Size: 329 259
AhdI GACnn_n'nnGTC
Cuts at: 0 336 588
Size: 336 252
AleI CACnn'nnGTG
Cuts at: 0 580 588
Size: 580 8
AvaI C'yCGr_G
Cuts at: 0 223 588
Size: 223 365
BanII G_rGCy'C
Cuts at: 0 195 588
Size: 195 393
BciVI GTATCCnnnnn_n'
Cuts at: 0 31 588
Size: 31 557
Bme1580I G_kGCm'C
Cuts at: 0 224 588
Size: 224 364
BmgBI CAC'GTC
Cuts at: 0 338 588
Size: 338 250
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 168 588
Size: 168 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 163 588
Size: 115 48 425
Fragments arranged by size:
425 115 48
BsaWI w'CCGG_w
Cuts at: 0 555 588
Size: 555 33
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 16 529 588
Size: 16 513 59
Fragments arranged by size:
513 59 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 588
Size: 369 219
BsiHKAI G_wGCw'C
Cuts at: 0 195 588
Size: 195 393
Bsp1286I G_dGCh'C
Cuts at: 0 195 224 588
Size: 195 29 364
Fragments arranged by size:
364 195 29
BspEI T'CCGG_A
Cuts at: 0 555 588
Size: 555 33
BsrFI r'CCGG_y
Cuts at: 0 388 588
Size: 388 200
BssSI C'ACGA_G
Cuts at: 0 402 588
Size: 402 186
BstAPI GCAn_nnn'nTGC
Cuts at: 0 392 588
Size: 392 196
BstYI r'GATC_y
Cuts at: 0 71 110 588
Size: 71 39 478
Fragments arranged by size:
478 71 39
BtsI GCAGTG_nn'
Cuts at: 0 414 588
Size: 414 174
EaeI y'GGCC_r
Cuts at: 0 56 455 588
Size: 56 399 133
Fragments arranged by size:
399 133 56
EarI CTCTTCn'nnn_
Cuts at: 0 247 563 588
Size: 247 316 25
Fragments arranged by size:
316 247 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 330 588
Size: 330 258
EcoICRI GAG'CTC
Cuts at: 0 193 588
Size: 193 395
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 163 588
Size: 115 48 425
Fragments arranged by size:
425 115 48
EcoO109I rG'GnC_Cy
Cuts at: 0 5 126 588
Size: 5 121 462
Fragments arranged by size:
462 121 5
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 588
Size: 322 32 234
Fragments arranged by size:
322 234 32
HincII GTy'rAC
Cuts at: 0 330 588
Size: 330 258
MscI TGG'CCA
Cuts at: 0 457 588
Size: 457 131
MslI CAynn'nnrTG
Cuts at: 0 580 588
Size: 580 8
MspA1I CmG'CkG
Cuts at: 0 280 588
Size: 280 308
NspI r_CATG'y
Cuts at: 0 220 588
Size: 220 368
PflMI CCAn_nnn'nTGG
Cuts at: 0 54 588
Size: 54 534
PfoI T'CCnGG_A
Cuts at: 0 122 140 588
Size: 122 18 448
Fragments arranged by size:
448 122 18
PpuMI rG'GwC_Cy
Cuts at: 0 5 126 588
Size: 5 121 462
Fragments arranged by size:
462 121 5
SacI G_AGCT'C
Cuts at: 0 195 588
Size: 195 393
SalI G'TCGA_C
Cuts at: 0 328 588
Size: 328 260
StyI C'CwwG_G
Cuts at: 0 8 458 588
Size: 8 450 130
Fragments arranged by size:
450 130 8
Enzymes that do cut:
AccI AhdI AleI AvaI BanII BciVI Bme1580I BmgBI
BplI BpmI BsaWI BseRI BsgI BsiHKAI Bsp1286I BspEI
BsrFI BssSI BstAPI BstYI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I Hin4I HincII MscI MslI MspA1I
NspI PflMI PfoI PpuMI SacI SalI StyI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BclI BfrBI BglI BglII BlpI BmrI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI
BseYI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI