Restriction map for pF1KB7261
 (Linear) (Six-base) MAPSORT of: KIBB7261.seq  Check: 3653  from: 1  to: 588



KIBB7261.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AccI GT'mk_AC

Cuts at:      0     329     588 
   Size:        329     259
 
AhdI GACnn_n'nnGTC

Cuts at:      0     336     588 
   Size:        336     252
 
AleI CACnn'nnGTG

Cuts at:      0     580     588 
   Size:        580       8
 
AvaI C'yCGr_G

Cuts at:      0     223     588 
   Size:        223     365
 
BanII G_rGCy'C

Cuts at:      0     195     588 
   Size:        195     393
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      31     588 
   Size:         31     557
 
Bme1580I G_kGCm'C

Cuts at:      0     224     588 
   Size:        224     364
 
BmgBI CAC'GTC

Cuts at:      0     338     588 
   Size:        338     250
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     168     588 
   Size:        168     420
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     163     588 
   Size:        115      48     425

  Fragments arranged by size: 

                 425     115      48
 
BsaWI w'CCGG_w

Cuts at:      0     555     588 
   Size:        555      33
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      16     529     588 
   Size:         16     513      59

  Fragments arranged by size: 

                 513      59      16
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     369     588 
   Size:        369     219
 
BsiHKAI G_wGCw'C

Cuts at:      0     195     588 
   Size:        195     393
 
Bsp1286I G_dGCh'C

Cuts at:      0     195     224     588 
   Size:        195      29     364

  Fragments arranged by size: 

                 364     195      29
 
BspEI T'CCGG_A

Cuts at:      0     555     588 
   Size:        555      33
 
BsrFI r'CCGG_y

Cuts at:      0     388     588 
   Size:        388     200
 
BssSI C'ACGA_G

Cuts at:      0     402     588 
   Size:        402     186
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     392     588 
   Size:        392     196
 
BstYI r'GATC_y

Cuts at:      0      71     110     588 
   Size:         71      39     478

  Fragments arranged by size: 

                 478      71      39
 
BtsI GCAGTG_nn'

Cuts at:      0     414     588 
   Size:        414     174
 
EaeI y'GGCC_r

Cuts at:      0      56     455     588 
   Size:         56     399     133

  Fragments arranged by size: 

                 399     133      56
 
EarI CTCTTCn'nnn_

Cuts at:      0     247     563     588 
   Size:        247     316      25

  Fragments arranged by size: 

                 316     247      25
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     330     588 
   Size:        330     258
 
EcoICRI GAG'CTC

Cuts at:      0     193     588 
   Size:        193     395
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     115     163     588 
   Size:        115      48     425

  Fragments arranged by size: 

                 425     115      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0       5     126     588 
   Size:          5     121     462

  Fragments arranged by size: 

                 462     121       5
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     322     354     588 
   Size:        322      32     234

  Fragments arranged by size: 

                 322     234      32
 
HincII GTy'rAC

Cuts at:      0     330     588 
   Size:        330     258
 
MscI TGG'CCA

Cuts at:      0     457     588 
   Size:        457     131
 
MslI CAynn'nnrTG

Cuts at:      0     580     588 
   Size:        580       8
 
MspA1I CmG'CkG

Cuts at:      0     280     588 
   Size:        280     308
 
NspI r_CATG'y

Cuts at:      0     220     588 
   Size:        220     368
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      54     588 
   Size:         54     534
 
PfoI T'CCnGG_A

Cuts at:      0     122     140     588 
   Size:        122      18     448

  Fragments arranged by size: 

                 448     122      18
 
PpuMI rG'GwC_Cy

Cuts at:      0       5     126     588 
   Size:          5     121     462

  Fragments arranged by size: 

                 462     121       5
 
SacI G_AGCT'C

Cuts at:      0     195     588 
   Size:        195     393
 
SalI G'TCGA_C

Cuts at:      0     328     588 
   Size:        328     260
 
StyI C'CwwG_G

Cuts at:      0       8     458     588 
   Size:          8     450     130

  Fragments arranged by size: 

                 450     130       8
 

 Enzymes that do cut:

     AccI     AhdI     AleI     AvaI    BanII    BciVI Bme1580I    BmgBI
     BplI     BpmI    BsaWI    BseRI     BsgI  BsiHKAI Bsp1286I    BspEI
    BsrFI    BssSI   BstAPI    BstYI     BtsI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    Hin4I   HincII     MscI     MslI   MspA1I
     NspI    PflMI     PfoI    PpuMI     SacI     SalI     StyI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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