(Linear) (Six-base) MAPSORT of: KIBB7261.seq Check: 3653 from: 1 to: 588 KIBB7261.orf With 182 enzymes: SgfI * March 3, 2008 11:00 .. AccI GT'mk_AC Cuts at: 0 329 588 Size: 329 259 AhdI GACnn_n'nnGTC Cuts at: 0 336 588 Size: 336 252 AleI CACnn'nnGTG Cuts at: 0 580 588 Size: 580 8 AvaI C'yCGr_G Cuts at: 0 223 588 Size: 223 365 BanII G_rGCy'C Cuts at: 0 195 588 Size: 195 393 BciVI GTATCCnnnnn_n' Cuts at: 0 31 588 Size: 31 557 Bme1580I G_kGCm'C Cuts at: 0 224 588 Size: 224 364 BmgBI CAC'GTC Cuts at: 0 338 588 Size: 338 250 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 168 588 Size: 168 420 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 163 588 Size: 115 48 425 Fragments arranged by size: 425 115 48 BsaWI w'CCGG_w Cuts at: 0 555 588 Size: 555 33 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 16 529 588 Size: 16 513 59 Fragments arranged by size: 513 59 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 369 588 Size: 369 219 BsiHKAI G_wGCw'C Cuts at: 0 195 588 Size: 195 393 Bsp1286I G_dGCh'C Cuts at: 0 195 224 588 Size: 195 29 364 Fragments arranged by size: 364 195 29 BspEI T'CCGG_A Cuts at: 0 555 588 Size: 555 33 BsrFI r'CCGG_y Cuts at: 0 388 588 Size: 388 200 BssSI C'ACGA_G Cuts at: 0 402 588 Size: 402 186 BstAPI GCAn_nnn'nTGC Cuts at: 0 392 588 Size: 392 196 BstYI r'GATC_y Cuts at: 0 71 110 588 Size: 71 39 478 Fragments arranged by size: 478 71 39 BtsI GCAGTG_nn' Cuts at: 0 414 588 Size: 414 174 EaeI y'GGCC_r Cuts at: 0 56 455 588 Size: 56 399 133 Fragments arranged by size: 399 133 56 EarI CTCTTCn'nnn_ Cuts at: 0 247 563 588 Size: 247 316 25 Fragments arranged by size: 316 247 25 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 330 588 Size: 330 258 EcoICRI GAG'CTC Cuts at: 0 193 588 Size: 193 395 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 115 163 588 Size: 115 48 425 Fragments arranged by size: 425 115 48 EcoO109I rG'GnC_Cy Cuts at: 0 5 126 588 Size: 5 121 462 Fragments arranged by size: 462 121 5 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 322 354 588 Size: 322 32 234 Fragments arranged by size: 322 234 32 HincII GTy'rAC Cuts at: 0 330 588 Size: 330 258 MscI TGG'CCA Cuts at: 0 457 588 Size: 457 131 MslI CAynn'nnrTG Cuts at: 0 580 588 Size: 580 8 MspA1I CmG'CkG Cuts at: 0 280 588 Size: 280 308 NspI r_CATG'y Cuts at: 0 220 588 Size: 220 368 PflMI CCAn_nnn'nTGG Cuts at: 0 54 588 Size: 54 534 PfoI T'CCnGG_A Cuts at: 0 122 140 588 Size: 122 18 448 Fragments arranged by size: 448 122 18 PpuMI rG'GwC_Cy Cuts at: 0 5 126 588 Size: 5 121 462 Fragments arranged by size: 462 121 5 SacI G_AGCT'C Cuts at: 0 195 588 Size: 195 393 SalI G'TCGA_C Cuts at: 0 328 588 Size: 328 260 StyI C'CwwG_G Cuts at: 0 8 458 588 Size: 8 450 130 Fragments arranged by size: 450 130 8 Enzymes that do cut: AccI AhdI AleI AvaI BanII BciVI Bme1580I BmgBI BplI BpmI BsaWI BseRI BsgI BsiHKAI Bsp1286I BspEI BsrFI BssSI BstAPI BstYI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoO109I Hin4I HincII MscI MslI MspA1I NspI PflMI PfoI PpuMI SacI SalI StyI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI