Restriction map for
pF1KB7269
(Linear) (Six-base) MAPSORT of: KIBB7269.seq Check: 401 from: 1 to: 1293
KIBB7269.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AclI AA'CG_TT
Cuts at: 0 335 1293
Size: 335 958
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 308 391 1088 1293
Size: 69 239 83 697 205
Fragments arranged by size:
697 239 205 83 69
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 962 994 1293
Size: 962 32 299
Fragments arranged by size:
962 299 32
ApaI G_GGCC'C
Cuts at: 0 193 593 1293
Size: 193 400 700
Fragments arranged by size:
700 400 193
ApoI r'AATT_y
Cuts at: 0 824 1164 1293
Size: 824 340 129
Fragments arranged by size:
824 340 129
BamHI G'GATC_C
Cuts at: 0 656 1090 1293
Size: 656 434 203
Fragments arranged by size:
656 434 203
BanII G_rGCy'C
Cuts at: 0 193 593 1293
Size: 193 400 700
Fragments arranged by size:
700 400 193
BbsI GAAGACnn'nnnn_
Cuts at: 0 1218 1293
Size: 1218 75
Bme1580I G_kGCm'C
Cuts at: 0 193 593 1293
Size: 193 400 700
Fragments arranged by size:
700 400 193
BmrI ACTGGGnnnn_n'
Cuts at: 0 506 842 926 1044 1255 1293
Size: 506 336 84 118 211 38
Fragments arranged by size:
506 336 211 118 84 38
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 343 775 1293
Size: 343 432 518
Fragments arranged by size:
518 432 343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 187 686 1046 1069 1293
Size: 98 89 499 360 23 224
Fragments arranged by size:
499 360 224 98 89 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 104 1047 1278 1293
Size: 104 943 231 15
Fragments arranged by size:
943 231 104 15
BsiHKAI G_wGCw'C
Cuts at: 0 1057 1148 1293
Size: 1057 91 145
Fragments arranged by size:
1057 145 91
BsmI GAATG_Cn'
Cuts at: 0 314 1293
Size: 314 979
Bsp1286I G_dGCh'C
Cuts at: 0 193 593 1057 1148 1293
Size: 193 400 464 91 145
Fragments arranged by size:
464 400 193 145 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 156 734 1293
Size: 156 578 559
Fragments arranged by size:
578 559 156
BsrGI T'GTAC_A
Cuts at: 0 561 1293
Size: 561 732
BstAPI GCAn_nnn'nTGC
Cuts at: 0 309 1293
Size: 309 984
BstXI CCAn_nnnn'nTGG
Cuts at: 0 200 1293
Size: 200 1093
BstYI r'GATC_y
Cuts at: 0 195 656 1090 1293
Size: 195 461 434 203
Fragments arranged by size:
461 434 203 195
BtgI C'CryG_G
Cuts at: 0 184 1293
Size: 184 1109
DraI TTT'AAA
Cuts at: 0 357 1293
Size: 357 936
DraIII CAC_nnn'GTG
Cuts at: 0 1002 1293
Size: 1002 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 7 1293
Size: 7 1286
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 98 187 308 391 686 1046 1069
Size: 69 29 89 121 83 295 360 23
Cuts at: 1069 1088 1293
Size: 19 205
Fragments arranged by size:
360 295 205 121 89 83 69 29
23 19
EcoO109I rG'GnC_Cy
Cuts at: 0 189 589 590 1023 1293
Size: 189 400 1 433 270
Fragments arranged by size:
433 400 270 189 1
EcoRI G'AATT_C
Cuts at: 0 824 1293
Size: 824 469
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 369 401 633 665 1293
Size: 369 32 232 32 628
Fragments arranged by size:
628 369 232 32 32
HindIII A'AGCT_T
Cuts at: 0 469 533 785 1293
Size: 469 64 252 508
Fragments arranged by size:
508 469 252 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1293
Size: 760 533
NcoI C'CATG_G
Cuts at: 0 184 1293
Size: 184 1109
NspI r_CATG'y
Cuts at: 0 1141 1293
Size: 1141 152
PasI CC'CwG_GG
Cuts at: 0 200 1293
Size: 200 1093
PfoI T'CCnGG_A
Cuts at: 0 244 1293
Size: 244 1049
PpuMI rG'GwC_Cy
Cuts at: 0 1023 1293
Size: 1023 270
PsiI TTA'TAA
Cuts at: 0 271 1293
Size: 271 1022
PspOMI G'GGCC_C
Cuts at: 0 189 589 1293
Size: 189 400 704
Fragments arranged by size:
704 400 189
PstI C_TGCA'G
Cuts at: 0 731 1293
Size: 731 562
SfcI C'TryA_G
Cuts at: 0 727 1293
Size: 727 566
StyI C'CwwG_G
Cuts at: 0 69 184 1026 1293
Size: 69 115 842 267
Fragments arranged by size:
842 267 115 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 892 1293
Size: 892 401
TatI w'GTAC_w
Cuts at: 0 561 1293
Size: 561 732
XmnI GAAnn'nnTTC
Cuts at: 0 451 787 955 1168 1197 1293
Size: 451 336 168 213 29 96
Fragments arranged by size:
451 336 213 168 96 29
Enzymes that do cut:
AclI AcuI AloI ApaI ApoI BamHI BanII BbsI
Bme1580I BmrI BplI BpmI BseRI BsiHKAI BsmI Bsp1286I
BspMI BsrGI BstAPI BstXI BstYI BtgI DraI DraIII
EciI Eco57MI EcoO109I EcoRI Hin4I HindIII MmeI NcoI
NspI PasI PfoI PpuMI PsiI PspOMI PstI SfcI
StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DrdI EaeI EagI EarI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PshAI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI