Restriction map for
pF1KB7271
(Linear) (Six-base) MAPSORT of: KIBB7271.seq Check: 3645 from: 1 to: 1485
KIBB7271.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 52 1106 1485
Size: 52 1054 379
Fragments arranged by size:
1054 379 52
AflII C'TTAA_G
Cuts at: 0 1180 1485
Size: 1180 305
AleI CACnn'nnGTG
Cuts at: 0 199 1485
Size: 199 1286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 227 1485
Size: 227 1258
AlwNI CAG_nnn'CTG
Cuts at: 0 570 1480 1485
Size: 570 910 5
Fragments arranged by size:
910 570 5
AvaI C'yCGr_G
Cuts at: 0 1449 1485
Size: 1449 36
BanI G'GyrC_C
Cuts at: 0 1370 1485
Size: 1370 115
BanII G_rGCy'C
Cuts at: 0 115 283 393 496 990 1450 1459 1485
Size: 115 168 110 103 494 460 9 26
Fragments arranged by size:
494 460 168 115 110 103 26 9
BbeI G_GCGC'C
Cuts at: 0 1374 1485
Size: 1374 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 1162 1485
Size: 1162 323
BglI GCCn_nnn'nGGC
Cuts at: 0 382 1485
Size: 382 1103
Bme1580I G_kGCm'C
Cuts at: 0 440 1318 1485
Size: 440 878 167
Fragments arranged by size:
878 440 167
BmrI ACTGGGnnnn_n'
Cuts at: 0 494 864 1485
Size: 494 370 621
Fragments arranged by size:
621 494 370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 349 509 899 1049 1485
Size: 43 306 160 390 150 436
Fragments arranged by size:
436 390 306 160 150 43
Bpu10I CC'TnA_GC
Cuts at: 0 905 1485
Size: 905 580
BsaI GGTCTCn'nnnn_
Cuts at: 0 885 1485
Size: 885 600
BsaHI Gr'CG_yC
Cuts at: 0 1371 1485
Size: 1371 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1291 1321 1485
Size: 1291 30 164
Fragments arranged by size:
1291 164 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 260 263 1485
Size: 260 3 1222
Fragments arranged by size:
1222 260 3
BseYI C'CCAG_C
Cuts at: 0 420 439 1229 1485
Size: 420 19 790 256
Fragments arranged by size:
790 420 256 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 731 1031 1485
Size: 731 300 454
Fragments arranged by size:
731 454 300
BsiHKAI G_wGCw'C
Cuts at: 0 115 393 1485
Size: 115 278 1092
Fragments arranged by size:
1092 278 115
Bsp1286I G_dGCh'C
Cuts at: 0 115 283 393 440 496 990 1318 1450
Size: 115 168 110 47 56 494 328 132
Cuts at: 1450 1459 1485
Size: 9 26
Fragments arranged by size:
494 328 168 132 115 110 56 47
26 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 758 951 1485
Size: 758 193 534
Fragments arranged by size:
758 534 193
BsrBI CCG'CTC
Cuts at: 0 398 1485
Size: 398 1087
BsrFI r'CCGG_y
Cuts at: 0 533 1485
Size: 533 952
BsrGI T'GTAC_A
Cuts at: 0 346 1485
Size: 346 1139
BssSI C'ACGA_G
Cuts at: 0 289 1485
Size: 289 1196
BstEII G'GTnAC_C
Cuts at: 0 139 1485
Size: 139 1346
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1023 1485
Size: 1023 462
BstYI r'GATC_y
Cuts at: 0 127 1391 1485
Size: 127 1264 94
Fragments arranged by size:
1264 127 94
Bsu36I CC'TnA_GG
Cuts at: 0 953 1100 1485
Size: 953 147 385
Fragments arranged by size:
953 385 147
BtgI C'CryG_G
Cuts at: 0 353 1485
Size: 353 1132
BtsI GCAGTG_nn'
Cuts at: 0 1350 1485
Size: 1350 135
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 645 680 1485
Size: 645 35 805
Fragments arranged by size:
805 645 35
EaeI y'GGCC_r
Cuts at: 0 383 1485
Size: 383 1102
EarI CTCTTCn'nnn_
Cuts at: 0 554 1485
Size: 554 931
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 986 1485
Size: 986 499
EcoICRI GAG'CTC
Cuts at: 0 113 391 1485
Size: 113 278 1094
Fragments arranged by size:
1094 278 113
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 349 509 899 1049 1485
Size: 43 306 160 390 150 436
Fragments arranged by size:
436 390 306 160 150 43
EcoNI CCTnn'n_nnAGG
Cuts at: 0 904 979 1485
Size: 904 75 506
Fragments arranged by size:
904 506 75
EcoO109I rG'GnC_Cy
Cuts at: 0 940 1485
Size: 940 545
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 71 583 1179 1485
Size: 71 512 596 306
Fragments arranged by size:
596 512 306 71
FspI TGC'GCA
Cuts at: 0 1342 1485
Size: 1342 143
HaeII r_GCGC'y
Cuts at: 0 922 1374 1485
Size: 922 452 111
Fragments arranged by size:
922 452 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 270 302 1255 1287 1485
Size: 270 32 953 32 198
Fragments arranged by size:
953 270 198 32 32
KasI G'GCGC_C
Cuts at: 0 1370 1485
Size: 1370 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 1485
Size: 501 984
MscI TGG'CCA
Cuts at: 0 385 1485
Size: 385 1100
MslI CAynn'nnrTG
Cuts at: 0 199 209 1485
Size: 199 10 1276
Fragments arranged by size:
1276 199 10
MspA1I CmG'CkG
Cuts at: 0 1229 1485
Size: 1229 256
NaeI GCC'GGC
Cuts at: 0 535 1485
Size: 535 950
NarI GG'CG_CC
Cuts at: 0 1371 1485
Size: 1371 114
NdeI CA'TA_TG
Cuts at: 0 7 206 1485
Size: 7 199 1279
Fragments arranged by size:
1279 199 7
NgoMIV G'CCGG_C
Cuts at: 0 533 1485
Size: 533 952
NspI r_CATG'y
Cuts at: 0 347 843 1366 1473 1485
Size: 347 496 523 107 12
Fragments arranged by size:
523 496 347 107 12
PasI CC'CwG_GG
Cuts at: 0 981 1485
Size: 981 504
PfoI T'CCnGG_A
Cuts at: 0 20 1485
Size: 20 1465
PpuMI rG'GwC_Cy
Cuts at: 0 940 1485
Size: 940 545
PstI C_TGCA'G
Cuts at: 0 454 1228 1443 1485
Size: 454 774 215 42
Fragments arranged by size:
774 454 215 42
PvuII CAG'CTG
Cuts at: 0 1229 1485
Size: 1229 256
SacI G_AGCT'C
Cuts at: 0 115 393 1485
Size: 115 278 1092
Fragments arranged by size:
1092 278 115
SapI GCTCTTCn'nnn_
Cuts at: 0 554 1485
Size: 554 931
SbfI CC_TGCA'GG
Cuts at: 0 454 1485
Size: 454 1031
SfcI C'TryA_G
Cuts at: 0 450 1224 1439 1485
Size: 450 774 215 46
Fragments arranged by size:
774 450 215 46
SfoI GGC'GCC
Cuts at: 0 1372 1485
Size: 1372 113
SmaI CCC'GGG
Cuts at: 0 1451 1485
Size: 1451 34
SmlI C'TyrA_G
Cuts at: 0 1180 1485
Size: 1180 305
SphI G_CATG'C
Cuts at: 0 1366 1473 1485
Size: 1366 107 12
Fragments arranged by size:
1366 107 12
StyI C'CwwG_G
Cuts at: 0 458 1485
Size: 458 1027
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1343 1485
Size: 1343 142
TatI w'GTAC_w
Cuts at: 0 346 1485
Size: 346 1139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 932 964 1485
Size: 932 32 521
Fragments arranged by size:
932 521 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 204 1485
Size: 204 1281
XmaI C'CCGG_G
Cuts at: 0 1449 1485
Size: 1449 36
XmnI GAAnn'nnTTC
Cuts at: 0 1216 1485
Size: 1216 269
Enzymes that do cut:
AccI AflII AleI AlfI AlwNI AvaI BanI BanII
BbeI BbsI BglI Bme1580I BmrI BpmI Bpu10I BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I
BtgI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI FspI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI
PasI PfoI PpuMI PstI PvuII SacI SapI SbfI
SfcI SfoI SmaI SmlI SphI StyI TaqII TatI
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BssHII BstAPI BstBI BstZ17I BtgZI ClaI DraI DraIII
DrdI EagI EcoRI EcoRV FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI NcoI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI ZraI