Restriction map for
pF1KB7283
(Linear) (Six-base) MAPSORT of: KIBB7283.seq Check: 3667 from: 1 to: 2670
KIBB7283.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 417 2670
Size: 417 2253
AatII G_ACGT'C
Cuts at: 0 1535 2670
Size: 1535 1135
Acc65I G'GTAC_C
Cuts at: 0 953 1286 2670
Size: 953 333 1384
Fragments arranged by size:
1384 953 333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 677 1477 2670
Size: 677 800 1193
Fragments arranged by size:
1193 800 677
AflIII A'CryG_T
Cuts at: 0 2001 2670
Size: 2001 669
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 587 1948 2670
Size: 587 1361 722
Fragments arranged by size:
1361 722 587
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2269 2301 2670
Size: 2269 32 369
Fragments arranged by size:
2269 369 32
AlwNI CAG_nnn'CTG
Cuts at: 0 420 2599 2641 2670
Size: 420 2179 42 29
Fragments arranged by size:
2179 420 42 29
ApaI G_GGCC'C
Cuts at: 0 2512 2670
Size: 2512 158
ApoI r'AATT_y
Cuts at: 0 517 607 1125 1696 2670
Size: 517 90 518 571 974
Fragments arranged by size:
974 571 518 517 90
AseI AT'TA_AT
Cuts at: 0 1928 2670
Size: 1928 742
AvaI C'yCGr_G
Cuts at: 0 147 833 1661 2300 2670
Size: 147 686 828 639 370
Fragments arranged by size:
828 686 639 370 147
BanI G'GyrC_C
Cuts at: 0 538 953 1286 2670
Size: 538 415 333 1384
Fragments arranged by size:
1384 538 415 333
BanII G_rGCy'C
Cuts at: 0 72 108 503 1900 2512 2670
Size: 72 36 395 1397 612 158
Fragments arranged by size:
1397 612 395 158 72 36
BbvCI CC'TCA_GC
Cuts at: 0 839 949 2065 2317 2670
Size: 839 110 1116 252 353
Fragments arranged by size:
1116 839 353 252 110
BciVI GTATCCnnnnn_n'
Cuts at: 0 655 2670
Size: 655 2015
BclI T'GATC_A
Cuts at: 0 2231 2670
Size: 2231 439
BfrBI ATG'CAT
Cuts at: 0 1153 2670
Size: 1153 1517
BglI GCCn_nnn'nGGC
Cuts at: 0 1922 2670
Size: 1922 748
BglII A'GATC_T
Cuts at: 0 2201 2670
Size: 2201 469
BlpI GC'TnA_GC
Cuts at: 0 107 2670
Size: 107 2563
Bme1580I G_kGCm'C
Cuts at: 0 1661 2512 2670
Size: 1661 851 158
Fragments arranged by size:
1661 851 158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 282 2670
Size: 282 2388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 442 1450 1895 2670
Size: 354 88 1008 445 775
Fragments arranged by size:
1008 775 445 354 88
Bpu10I CC'TnA_GC
Cuts at: 0 502 839 949 2065 2317 2670
Size: 502 337 110 1116 252 353
Fragments arranged by size:
1116 502 353 337 252 110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 1525 2670
Size: 42 1483 1145
Fragments arranged by size:
1483 1145 42
BsaI GGTCTCn'nnnn_
Cuts at: 0 158 2158 2449 2652 2670
Size: 158 2000 291 203 18
Fragments arranged by size:
2000 291 203 158 18
BsaHI Gr'CG_yC
Cuts at: 0 1532 2670
Size: 1532 1138
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 313 343 379 409 2448 2478 2670
Size: 313 30 36 30 2039 30 192
Fragments arranged by size:
2039 313 192 36 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 330 337 415 2453 2670
Size: 330 7 78 2038 217
Fragments arranged by size:
2038 330 217 78 7
BseYI C'CCAG_C
Cuts at: 0 42 198 1106 1467 1673 1749 1802 1860
Size: 42 156 908 361 206 76 53 58
Cuts at: 1860 1967 2238 2312 2432 2670
Size: 107 271 74 120 238
Fragments arranged by size:
908 361 271 238 206 156 120 107
76 74 58 53 42
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 887 1627 2670
Size: 887 740 1043
Fragments arranged by size:
1043 887 740
BsiEI CG_ry'CG
Cuts at: 0 1027 2670
Size: 1027 1643
BsiHKAI G_wGCw'C
Cuts at: 0 108 503 2060 2339 2655 2670
Size: 108 395 1557 279 316 15
Fragments arranged by size:
1557 395 316 279 108 15
Bsp1286I G_dGCh'C
Cuts at: 0 72 108 503 1661 1900 2060 2339 2512
Size: 72 36 395 1158 239 160 279 173
Cuts at: 2512 2655 2670
Size: 143 15
Fragments arranged by size:
1158 395 279 239 173 160 143 72
36 15
BspHI T'CATG_A
Cuts at: 0 1700 1784 2670
Size: 1700 84 886
Fragments arranged by size:
1700 886 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 241 417 2670
Size: 241 176 2253
Fragments arranged by size:
2253 241 176
BsrBI CCG'CTC
Cuts at: 0 213 279 2670
Size: 213 66 2391
Fragments arranged by size:
2391 213 66
BsrDI GCAATG_nn'
Cuts at: 0 449 2670
Size: 449 2221
BsrFI r'CCGG_y
Cuts at: 0 956 2076 2670
Size: 956 1120 594
Fragments arranged by size:
1120 956 594
BssHII G'CGCG_C
Cuts at: 0 126 2670
Size: 126 2544
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1749 2670
Size: 1749 921
BstYI r'GATC_y
Cuts at: 0 52 416 2201 2670
Size: 52 364 1785 469
Fragments arranged by size:
1785 469 364 52
BtgI C'CryG_G
Cuts at: 0 479 854 2489 2626 2670
Size: 479 375 1635 137 44
Fragments arranged by size:
1635 479 375 137 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1404 2670
Size: 1404 1266
BtsI GCAGTG_nn'
Cuts at: 0 2542 2670
Size: 2542 128
ClaI AT'CG_AT
Cuts at: 0 1027 2670
Size: 1027 1643
EaeI y'GGCC_r
Cuts at: 0 590 1031 1247 2261 2601 2670
Size: 590 441 216 1014 340 69
Fragments arranged by size:
1014 590 441 340 216 69
EarI CTCTTCn'nnn_
Cuts at: 0 188 913 1492 1550 1611 2065 2347 2447
Size: 188 725 579 58 61 454 282 100
Cuts at: 2447 2670
Size: 223
Fragments arranged by size:
725 579 454 282 223 188 100 61
58
EcoICRI GAG'CTC
Cuts at: 0 106 501 2670
Size: 106 395 2169
Fragments arranged by size:
2169 395 106
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 442 677 1450 1477 1895 2670
Size: 354 88 235 773 27 418 775
Fragments arranged by size:
775 773 418 354 235 88 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 397 424 2670
Size: 25 372 27 2246
Fragments arranged by size:
2246 372 27 25
EcoO109I rG'GnC_Cy
Cuts at: 0 18 86 230 263 2508 2509 2516 2670
Size: 18 68 144 33 2245 1 7 154
Fragments arranged by size:
2245 154 144 68 33 18 7 1
EcoRI G'AATT_C
Cuts at: 0 1125 1696 2670
Size: 1125 571 974
Fragments arranged by size:
1125 974 571
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 636 2670
Size: 636 2034
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 313 345 379 411 816 848 2448 2480
Size: 313 32 34 32 405 32 1600 32
Cuts at: 2480 2670
Size: 190
Fragments arranged by size:
1600 405 313 190 34 32 32 32
32
HincII GTy'rAC
Cuts at: 0 738 2670
Size: 738 1932
HindIII A'AGCT_T
Cuts at: 0 1543 2670
Size: 1543 1127
HpaI GTT'AAC
Cuts at: 0 738 2670
Size: 738 1932
KpnI G_GTAC'C
Cuts at: 0 957 1290 2670
Size: 957 333 1380
Fragments arranged by size:
1380 957 333
MluI A'CGCG_T
Cuts at: 0 2001 2670
Size: 2001 669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1594 2048 2463 2670
Size: 1594 454 415 207
Fragments arranged by size:
1594 454 415 207
MscI TGG'CCA
Cuts at: 0 592 1033 1249 2263 2603 2670
Size: 592 441 216 1014 340 67
Fragments arranged by size:
1014 592 441 340 216 67
MslI CAynn'nnrTG
Cuts at: 0 1930 2092 2670
Size: 1930 162 578
Fragments arranged by size:
1930 578 162
MspA1I CmG'CkG
Cuts at: 0 1106 1138 1346 1467 1684 2316 2670
Size: 1106 32 208 121 217 632 354
Fragments arranged by size:
1106 632 354 217 208 121 32
NcoI C'CATG_G
Cuts at: 0 479 854 2489 2670
Size: 479 375 1635 181
Fragments arranged by size:
1635 479 375 181
NdeI CA'TA_TG
Cuts at: 0 1830 2670
Size: 1830 840
NsiI A_TGCA'T
Cuts at: 0 1155 2670
Size: 1155 1515
NspI r_CATG'y
Cuts at: 0 801 1725 2670
Size: 801 924 945
Fragments arranged by size:
945 924 801
PasI CC'CwG_GG
Cuts at: 0 650 2512 2670
Size: 650 1862 158
Fragments arranged by size:
1862 650 158
PflMI CCAn_nnn'nTGG
Cuts at: 0 420 599 944 1450 2490 2599 2670
Size: 420 179 345 506 1040 109 71
Fragments arranged by size:
1040 506 420 345 179 109 71
PfoI T'CCnGG_A
Cuts at: 0 272 419 2670
Size: 272 147 2251
Fragments arranged by size:
2251 272 147
PpuMI rG'GwC_Cy
Cuts at: 0 86 230 2516 2670
Size: 86 144 2286 154
Fragments arranged by size:
2286 154 144 86
PspOMI G'GGCC_C
Cuts at: 0 2508 2670
Size: 2508 162
PstI C_TGCA'G
Cuts at: 0 1653 2670
Size: 1653 1017
PvuI CG_AT'CG
Cuts at: 0 1027 2670
Size: 1027 1643
PvuII CAG'CTG
Cuts at: 0 1106 1138 1346 1467 1684 2316 2670
Size: 1106 32 208 121 217 632 354
Fragments arranged by size:
1106 632 354 217 208 121 32
SacI G_AGCT'C
Cuts at: 0 108 503 2670
Size: 108 395 2167
Fragments arranged by size:
2167 395 108
SanDI GG'GwC_CC
Cuts at: 0 86 2670
Size: 86 2584
SapI GCTCTTCn'nnn_
Cuts at: 0 188 2065 2670
Size: 188 1877 605
Fragments arranged by size:
1877 605 188
ScaI AGT'ACT
Cuts at: 0 898 2670
Size: 898 1772
SexAI A'CCwGG_T
Cuts at: 0 8 2670
Size: 8 2662
SfcI C'TryA_G
Cuts at: 0 1002 1649 2670
Size: 1002 647 1021
Fragments arranged by size:
1021 1002 647
SmaI CCC'GGG
Cuts at: 0 149 1663 2670
Size: 149 1514 1007
Fragments arranged by size:
1514 1007 149
SmlI C'TyrA_G
Cuts at: 0 57 1540 2670
Size: 57 1483 1130
Fragments arranged by size:
1483 1130 57
SphI G_CATG'C
Cuts at: 0 801 2670
Size: 801 1869
SspI AAT'ATT
Cuts at: 0 761 2670
Size: 761 1909
StuI AGG'CCT
Cuts at: 0 1916 2228 2670
Size: 1916 312 442
Fragments arranged by size:
1916 442 312
StyI C'CwwG_G
Cuts at: 0 362 479 542 854 1044 1878 2447 2489
Size: 362 117 63 312 190 834 569 42
Cuts at: 2489 2670
Size: 181
Fragments arranged by size:
834 569 362 312 190 181 117 63
42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1457 2446 2670
Size: 1457 989 224
Fragments arranged by size:
1457 989 224
TatI w'GTAC_w
Cuts at: 0 896 1955 2670
Size: 896 1059 715
Fragments arranged by size:
1059 896 715
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1608 1640 2670
Size: 1608 32 1030
Fragments arranged by size:
1608 1030 32
Tth111I GACn'n_nGTC
Cuts at: 0 1713 2670
Size: 1713 957
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1425 1447 2670
Size: 1425 22 1223
Fragments arranged by size:
1425 1223 22
XmaI C'CCGG_G
Cuts at: 0 147 1661 2670
Size: 147 1514 1009
Fragments arranged by size:
1514 1009 147
ZraI GAC'GTC
Cuts at: 0 1533 2670
Size: 1533 1137
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AlfI AloI AlwNI
ApaI ApoI AseI AvaI BanI BanII BbvCI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BssHII BstXI BstYI BtgI BtgZI BtsI ClaI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I
HincII HindIII HpaI KpnI MluI MmeI MscI MslI
MspA1I NcoI NdeI NsiI NspI PasI PflMI PfoI
PpuMI PspOMI PstI PvuI PvuII SacI SanDI SapI
ScaI SexAI SfcI SmaI SmlI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AleI ApaLI
AscI AsiSI AvrII BaeI BamHI BbeI BbsI BcgI
BmgBI BmrI BmtI BsaAI BsaBI BsaWI BsiWI BsmI
BsmBI BspEI BsrGI BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I CspCI DraI DraIII DrdI EagI EciI EcoRV
FseI FspI FspAI HaeII KasI MfeI NaeI NarI
NgoMIV NheI NotI NruI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI RsrII SacII SalI
SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SwaI XbaI XhoI XmnI