Restriction map for
pF1KB7294
(Linear) (Six-base) MAPSORT of: KIBB7294.seq Check: 3217 from: 1 to: 2307
KIBB7294.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 460 1396 2035 2307
Size: 460 936 639 272
Fragments arranged by size:
936 639 460 272
AccI GT'mk_AC
Cuts at: 0 941 2307
Size: 941 1366
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 333 2004 2307
Size: 333 1671 303
Fragments arranged by size:
1671 333 303
AflIII A'CryG_T
Cuts at: 0 1973 2307
Size: 1973 334
AhdI GACnn_n'nnGTC
Cuts at: 0 74 2307
Size: 74 2233
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1188 2307
Size: 1188 1119
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 415 447 1204 1236 2307
Size: 415 32 757 32 1071
Fragments arranged by size:
1071 757 415 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 198 462 1119 1131 1275 2008 2027 2307
Size: 198 264 657 12 144 733 19 280
Fragments arranged by size:
733 657 280 264 198 144 19 12
ApaI G_GGCC'C
Cuts at: 0 235 2307
Size: 235 2072
ApaLI G'TGCA_C
Cuts at: 0 700 2307
Size: 700 1607
AvaI C'yCGr_G
Cuts at: 0 990 1646 2307
Size: 990 656 661
Fragments arranged by size:
990 661 656
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2112 2145 2307
Size: 2112 33 162
Fragments arranged by size:
2112 162 33
BanI G'GyrC_C
Cuts at: 0 2194 2307
Size: 2194 113
BanII G_rGCy'C
Cuts at: 0 90 132 172 235 509 537 968 1162
Size: 90 42 40 63 274 28 431 194
Cuts at: 1162 1466 1827 2307
Size: 304 361 480
Fragments arranged by size:
480 431 361 304 274 194 90 63
42 40 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 465 2307
Size: 465 1842
BciVI GTATCCnnnnn_n'
Cuts at: 0 2140 2307
Size: 2140 167
BclI T'GATC_A
Cuts at: 0 734 2307
Size: 734 1573
BglI GCCn_nnn'nGGC
Cuts at: 0 637 2307
Size: 637 1670
Bme1580I G_kGCm'C
Cuts at: 0 235 704 1570 2307
Size: 235 469 866 737
Fragments arranged by size:
866 737 469 235
BmgBI CAC'GTC
Cuts at: 0 1785 2150 2307
Size: 1785 365 157
Fragments arranged by size:
1785 365 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 627 962 1226 2307
Size: 627 335 264 1081
Fragments arranged by size:
1081 627 335 264
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 322 407 937 1841 2307
Size: 104 218 85 530 904 466
Fragments arranged by size:
904 530 466 218 104 85
Bpu10I CC'TnA_GC
Cuts at: 0 962 1936 2307
Size: 962 974 371
Fragments arranged by size:
974 962 371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1300 2307
Size: 1300 1007
BsaI GGTCTCn'nnnn_
Cuts at: 0 383 913 1534 2307
Size: 383 530 621 773
Fragments arranged by size:
773 621 530 383
BsaAI yAC'GTr
Cuts at: 0 336 1032 1911 2307
Size: 336 696 879 396
Fragments arranged by size:
879 696 396 336
BsaHI Gr'CG_yC
Cuts at: 0 1445 2307
Size: 1445 862
BsaWI w'CCGG_w
Cuts at: 0 566 1154 2296 2307
Size: 566 588 1142 11
Fragments arranged by size:
1142 588 566 11
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 337 415 445 1895 1925 2307
Size: 307 30 78 30 1450 30 382
Fragments arranged by size:
1450 382 307 78 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 310 315 568 632 1381 1792 1817 2307
Size: 310 5 253 64 749 411 25 490
Fragments arranged by size:
749 490 411 310 253 64 25 5
BseYI C'CCAG_C
Cuts at: 0 17 110 114 148 270 368 1119 1194
Size: 17 93 4 34 122 98 751 75
Cuts at: 1194 1760 2188 2221 2264 2307
Size: 566 428 33 43 43
Fragments arranged by size:
751 566 428 122 98 93 75 43
43 34 33 17 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1408 1416 1473 1530 1564 1791 1933
Size: 922 486 8 57 57 34 227 142
Cuts at: 1933 2307
Size: 374
Fragments arranged by size:
922 486 374 227 142 57 57 34
8
BsiEI CG_ry'CG
Cuts at: 0 655 2307
Size: 655 1652
BsiHKAI G_wGCw'C
Cuts at: 0 90 172 704 1012 1162 1827 2307
Size: 90 82 532 308 150 665 480
Fragments arranged by size:
665 532 480 308 150 90 82
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2161 2307
Size: 2161 146
Bsp1286I G_dGCh'C
Cuts at: 0 90 132 172 235 509 537 704 968
Size: 90 42 40 63 274 28 167 264
Cuts at: 968 1012 1162 1466 1570 1827 2307
Size: 44 150 304 104 257 480
Fragments arranged by size:
480 304 274 264 257 167 150 104
90 63 44 42 40 28
BspEI T'CCGG_A
Cuts at: 0 2296 2307
Size: 2296 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 460 666 1235 1396 1552 1586 2035 2307
Size: 460 206 569 161 156 34 449 272
Fragments arranged by size:
569 460 449 272 206 161 156 34
BsrDI GCAATG_nn'
Cuts at: 0 2085 2216 2307
Size: 2085 131 91
Fragments arranged by size:
2085 131 91
BsrFI r'CCGG_y
Cuts at: 0 650 1652 2307
Size: 650 1002 655
Fragments arranged by size:
1002 655 650
BssSI C'ACGA_G
Cuts at: 0 1011 2307
Size: 1011 1296
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1418 1427 1785 1961 2123 2307
Size: 1418 9 358 176 162 184
Fragments arranged by size:
1418 358 184 176 162 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 720 804 1190 2250 2307
Size: 156 564 84 386 1060 57
Fragments arranged by size:
1060 564 386 156 84 57
BstYI r'GATC_y
Cuts at: 0 585 893 1753 2307
Size: 585 308 860 554
Fragments arranged by size:
860 585 554 308
Bsu36I CC'TnA_GG
Cuts at: 0 134 539 2307
Size: 134 405 1768
Fragments arranged by size:
1768 405 134
BtgI C'CryG_G
Cuts at: 0 803 866 1056 1492 2064 2159 2307
Size: 803 63 190 436 572 95 148
Fragments arranged by size:
803 572 436 190 148 95 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 108 1108 1518 1975 2307
Size: 108 1000 410 457 332
Fragments arranged by size:
1000 457 410 332 108
BtsI GCAGTG_nn'
Cuts at: 0 393 1663 2043 2307
Size: 393 1270 380 264
Fragments arranged by size:
1270 393 380 264
DraI TTT'AAA
Cuts at: 0 2274 2307
Size: 2274 33
DraIII CAC_nnn'GTG
Cuts at: 0 1498 2307
Size: 1498 809
DrdI GACnn_nn'nnGTC
Cuts at: 0 208 953 2307
Size: 208 745 1354
Fragments arranged by size:
1354 745 208
EaeI y'GGCC_r
Cuts at: 0 652 815 1097 2307
Size: 652 163 282 1210
Fragments arranged by size:
1210 652 282 163
EagI C'GGCC_G
Cuts at: 0 652 2307
Size: 652 1655
EarI CTCTTCn'nnn_
Cuts at: 0 991 1306 2307
Size: 991 315 1001
Fragments arranged by size:
1001 991 315
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 202 670 1936 2307
Size: 202 468 1266 371
Fragments arranged by size:
1266 468 371 202
EcoICRI GAG'CTC
Cuts at: 0 88 170 1160 1825 2307
Size: 88 82 990 665 482
Fragments arranged by size:
990 665 482 88 82
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 322 333 407 937 1841 2004 2307
Size: 104 218 11 74 530 904 163 303
Fragments arranged by size:
904 530 303 218 163 104 74 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1832 2307
Size: 1832 475
EcoO109I rG'GnC_Cy
Cuts at: 0 68 76 231 544 998 1839 2185 2307
Size: 68 8 155 313 454 841 346 122
Fragments arranged by size:
841 454 346 313 155 122 68 8
HaeII r_GCGC'y
Cuts at: 0 18 345 2049 2307
Size: 18 327 1704 258
Fragments arranged by size:
1704 327 258 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 1366 1398 2307
Size: 60 32 1274 32 909
Fragments arranged by size:
1274 909 60 32 32
HincII GTy'rAC
Cuts at: 0 1812 2307
Size: 1812 495
MspA1I CmG'CkG
Cuts at: 0 114 198 750 1019 1295 1478 1622 1917
Size: 114 84 552 269 276 183 144 295
Cuts at: 1917 2192 2307
Size: 275 115
Fragments arranged by size:
552 295 276 275 269 183 144 115
114 84
NcoI C'CATG_G
Cuts at: 0 866 2307
Size: 866 1441
NdeI CA'TA_TG
Cuts at: 0 1042 2307
Size: 1042 1265
NspI r_CATG'y
Cuts at: 0 1977 2307
Size: 1977 330
PciI A'CATG_T
Cuts at: 0 1973 2307
Size: 1973 334
PflMI CCAn_nnn'nTGG
Cuts at: 0 516 1119 2307
Size: 516 603 1188
Fragments arranged by size:
1188 603 516
PfoI T'CCnGG_A
Cuts at: 0 914 1322 2307
Size: 914 408 985
Fragments arranged by size:
985 914 408
PmlI CAC'GTG
Cuts at: 0 1911 2307
Size: 1911 396
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 2307
Size: 415 32 1860
Fragments arranged by size:
1860 415 32
PpuMI rG'GwC_Cy
Cuts at: 0 68 76 544 998 1839 2185 2307
Size: 68 8 468 454 841 346 122
Fragments arranged by size:
841 468 454 346 122 68 8
PspOMI G'GGCC_C
Cuts at: 0 231 2307
Size: 231 2076
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 478 510 2307
Size: 478 32 1797
Fragments arranged by size:
1797 478 32
PstI C_TGCA'G
Cuts at: 0 1280 1900 2077 2128 2307
Size: 1280 620 177 51 179
Fragments arranged by size:
1280 620 179 177 51
PvuII CAG'CTG
Cuts at: 0 114 198 750 1295 1917 2307
Size: 114 84 552 545 622 390
Fragments arranged by size:
622 552 545 390 114 84
SacI G_AGCT'C
Cuts at: 0 90 172 1162 1827 2307
Size: 90 82 990 665 480
Fragments arranged by size:
990 665 480 90 82
SanDI GG'GwC_CC
Cuts at: 0 68 544 2307
Size: 68 476 1763
Fragments arranged by size:
1763 476 68
SexAI A'CCwGG_T
Cuts at: 0 1841 2307
Size: 1841 466
SfcI C'TryA_G
Cuts at: 0 695 1248 1276 1896 2073 2124 2307
Size: 695 553 28 620 177 51 183
Fragments arranged by size:
695 620 553 183 177 51 28
SmaI CCC'GGG
Cuts at: 0 1648 2307
Size: 1648 659
SmlI C'TyrA_G
Cuts at: 0 1315 2307
Size: 1315 992
StyI C'CwwG_G
Cuts at: 0 72 526 866 2307
Size: 72 454 340 1441
Fragments arranged by size:
1441 454 340 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 994 2172 2307
Size: 994 1178 135
Fragments arranged by size:
1178 994 135
TatI w'GTAC_w
Cuts at: 0 1262 2307
Size: 1262 1045
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 1162 1194 1642 1674 1716 1748
Size: 307 32 823 32 448 32 42 32
Cuts at: 1748 1774 1806 1895 1927 2307
Size: 26 32 89 32 380
Fragments arranged by size:
823 448 380 307 89 42 32 32
32 32 32 32 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 79 463 517 2307
Size: 79 384 54 1790
Fragments arranged by size:
1790 384 79 54
XmaI C'CCGG_G
Cuts at: 0 1646 2307
Size: 1646 661
XmnI GAAnn'nnTTC
Cuts at: 0 1348 2307
Size: 1348 959
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI AvaI BaeI BanI BanII BbsI BciVI
BclI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII MspA1I NcoI
NdeI NspI PciI PflMI PfoI PmlI PpiI PpuMI
PspOMI PsrI PstI PvuII SacI SanDI SexAI SfcI
SmaI SmlI StyI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI ApoI
AscI AseI AsiSI AvrII BamHI BbeI BbvCI BcgI
BfrBI BglII BlpI BmtI BplI BsaBI BsiWI BsmI
BspHI BsrBI BsrGI BssHII BstBI BstEII BstZ17I ClaI
CspCI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NgoMIV NheI NotI NruI NsiI PacI
PasI PmeI PshAI PsiI PspXI PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I
XbaI XhoI ZraI