Restriction map for
pF1KB7295
(Linear) (Six-base) MAPSORT of: KIBB7295.seq Check: 7475 from: 1 to: 2667
KIBB7295.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1366 1434 2211 2307 2667
Size: 1366 68 777 96 360
Fragments arranged by size:
1366 777 360 96 68
AccI GT'mk_AC
Cuts at: 0 947 2667
Size: 947 1720
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 2667
Size: 612 2055
AflIII A'CryG_T
Cuts at: 0 1457 2667
Size: 1457 1210
AhdI GACnn_n'nnGTC
Cuts at: 0 885 2667
Size: 885 1782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1138 2667
Size: 1138 1529
AlwNI CAG_nnn'CTG
Cuts at: 0 1158 1404 2667
Size: 1158 246 1263
Fragments arranged by size:
1263 1158 246
ApaI G_GGCC'C
Cuts at: 0 139 146 341 349 362 401 451 1574
Size: 139 7 195 8 13 39 50 1123
Cuts at: 1574 2294 2321 2417 2549 2667
Size: 720 27 96 132 118
Fragments arranged by size:
1123 720 195 139 132 118 96 50
39 27 13 8 7
ApoI r'AATT_y
Cuts at: 0 1998 2667
Size: 1998 669
AscI GG'CGCG_CC
Cuts at: 0 1947 2345 2667
Size: 1947 398 322
Fragments arranged by size:
1947 398 322
AvaI C'yCGr_G
Cuts at: 0 28 39 132 139 167 261 340 393
Size: 28 11 93 7 28 94 79 53
Cuts at: 393 835 1303 1841 2037 2286 2314 2340 2360
Size: 442 468 538 196 249 28 26 20
Cuts at: 2360 2561 2667
Size: 201 106
Fragments arranged by size:
538 468 442 249 201 196 106 94
93 79 53 28 28 28 26 20
11 7
BanI G'GyrC_C
Cuts at: 0 256 1161 1299 1351 1418 1723 2364 2488
Size: 256 905 138 52 67 305 641 124
Cuts at: 2488 2608 2667
Size: 120 59
Fragments arranged by size:
905 641 305 256 138 124 120 67
59 52
BanII G_rGCy'C
Cuts at: 0 40 139 146 341 349 362 401 451
Size: 40 99 7 195 8 13 39 50
Cuts at: 451 1565 1574 2294 2321 2417 2549 2667
Size: 1114 9 720 27 96 132 118
Fragments arranged by size:
1114 720 195 132 118 99 96 50
40 39 27 13 9 8 7
BbeI G_GCGC'C
Cuts at: 0 1303 1422 2492 2612 2667
Size: 1303 119 1070 120 55
Fragments arranged by size:
1303 1070 120 119 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 1683 2667
Size: 1683 984
BbvCI CC'TCA_GC
Cuts at: 0 994 2667
Size: 994 1673
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 786 820 852 886 2667
Size: 786 34 32 34 1781
Fragments arranged by size:
1781 786 34 34 32
BglI GCCn_nnn'nGGC
Cuts at: 0 215 254 296 500 2289 2342 2667
Size: 215 39 42 204 1789 53 325
Fragments arranged by size:
1789 325 215 204 53 42 39
BglII A'GATC_T
Cuts at: 0 1055 1826 2667
Size: 1055 771 841
Fragments arranged by size:
1055 841 771
BlpI GC'TnA_GC
Cuts at: 0 1336 1897 2667
Size: 1336 561 770
Fragments arranged by size:
1336 770 561
Bme1580I G_kGCm'C
Cuts at: 0 139 146 341 349 362 401 451 1574
Size: 139 7 195 8 13 39 50 1123
Cuts at: 1574 2294 2321 2367 2417 2549 2667
Size: 720 27 46 50 132 118
Fragments arranged by size:
1123 720 195 139 132 118 50 50
46 39 27 13 8 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 648 682 1146 2667
Size: 648 34 464 1521
Fragments arranged by size:
1521 648 464 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1945 2667
Size: 1945 722
Bpu10I CC'TnA_GC
Cuts at: 0 994 2437 2667
Size: 994 1443 230
Fragments arranged by size:
1443 994 230
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1663 2667
Size: 1663 1004
BsaI GGTCTCn'nnnn_
Cuts at: 0 709 753 2667
Size: 709 44 1914
Fragments arranged by size:
1914 709 44
BsaAI yAC'GTr
Cuts at: 0 866 2667
Size: 866 1801
BsaBI GATnn'nnATC
Cuts at: 0 1096 2667
Size: 1096 1571
BsaHI Gr'CG_yC
Cuts at: 0 521 1300 1419 2489 2609 2667
Size: 521 779 119 1070 120 58
Fragments arranged by size:
1070 779 521 120 119 58
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 454 1066 1401 1597 1942 2667
Size: 439 15 612 335 196 345 725
Fragments arranged by size:
725 612 439 345 335 196 15
BseYI C'CCAG_C
Cuts at: 0 155 1193 2118 2410 2575 2667
Size: 155 1038 925 292 165 92
Fragments arranged by size:
1038 925 292 165 155 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 1996 2041 2125 2667
Size: 345 1651 45 84 542
Fragments arranged by size:
1651 542 345 84 45
BsiEI CG_ry'CG
Cuts at: 0 249 1293 1974 2094 2233 2667
Size: 249 1044 681 120 139 434
Fragments arranged by size:
1044 681 434 249 139 120
BsiHKAI G_wGCw'C
Cuts at: 0 277 1517 1565 1775 2667
Size: 277 1240 48 210 892
Fragments arranged by size:
1240 892 277 210 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1951 2667
Size: 1951 716
Bsp1286I G_dGCh'C
Cuts at: 0 40 139 146 277 341 349 362 401
Size: 40 99 7 131 64 8 13 39
Cuts at: 401 451 1517 1565 1574 1775 2294 2321 2367
Size: 50 1066 48 9 201 519 27 46
Cuts at: 2367 2417 2549 2667
Size: 50 132 118
Fragments arranged by size:
1066 519 201 132 131 118 99 64
50 50 48 46 40 39 27 13
9 8 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1366 1434 2154 2211 2307 2667
Size: 1366 68 720 57 96 360
Fragments arranged by size:
1366 720 360 96 68 57
BsrBI CCG'CTC
Cuts at: 0 2235 2667
Size: 2235 432
BsrFI r'CCGG_y
Cuts at: 0 218 417 793 1292 2614 2667
Size: 218 199 376 499 1322 53
Fragments arranged by size:
1322 499 376 218 199 53
BssHII G'CGCG_C
Cuts at: 0 7 503 1947 2345 2428 2641 2667
Size: 7 496 1444 398 83 213 26
Fragments arranged by size:
1444 496 398 213 83 26 7
BssSI C'ACGA_G
Cuts at: 0 240 375 1224 2236 2269 2667
Size: 240 135 849 1012 33 398
Fragments arranged by size:
1012 849 398 240 135 33
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2019 2667
Size: 2019 648
BstEII G'GTnAC_C
Cuts at: 0 2166 2667
Size: 2166 501
BstXI CCAn_nnnn'nTGG
Cuts at: 0 900 2667
Size: 900 1767
BstYI r'GATC_y
Cuts at: 0 989 1055 1097 1496 1826 2667
Size: 989 66 42 399 330 841
Fragments arranged by size:
989 841 399 330 66 42
BtgI C'CryG_G
Cuts at: 0 211 332 381 563 727 899 2368 2501
Size: 211 121 49 182 164 172 1469 133
Cuts at: 2501 2667
Size: 166
Fragments arranged by size:
1469 211 182 172 166 164 133 121
49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2163 2667
Size: 2163 504
BtsI GCAGTG_nn'
Cuts at: 0 328 719 1385 2667
Size: 328 391 666 1282
Fragments arranged by size:
1282 666 391 328
DraIII CAC_nnn'GTG
Cuts at: 0 1761 2019 2667
Size: 1761 258 648
Fragments arranged by size:
1761 648 258
DrdI GACnn_nn'nnGTC
Cuts at: 0 943 2667
Size: 943 1724
EaeI y'GGCC_r
Cuts at: 0 246 278 1079 1290 1377 1661 1964 2174
Size: 246 32 801 211 87 284 303 210
Cuts at: 2174 2201 2230 2519 2667
Size: 27 29 289 148
Fragments arranged by size:
801 303 289 284 246 211 210 148
87 32 29 27
EagI C'GGCC_G
Cuts at: 0 246 1290 2230 2667
Size: 246 1044 940 437
Fragments arranged by size:
1044 940 437 246
EarI CTCTTCn'nnn_
Cuts at: 0 1258 2143 2667
Size: 1258 885 524
Fragments arranged by size:
1258 885 524
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 211 436 1477 2451 2667
Size: 211 225 1041 974 216
Fragments arranged by size:
1041 974 225 216 211
EcoICRI GAG'CTC
Cuts at: 0 1563 2667
Size: 1563 1104
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 1945 2667
Size: 612 1333 722
Fragments arranged by size:
1333 722 612
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1579 2014 2667
Size: 1579 435 653
Fragments arranged by size:
1579 653 435
EcoO109I rG'GnC_Cy
Cuts at: 0 136 337 345 358 397 599 1571 2290
Size: 136 201 8 13 39 202 972 719
Cuts at: 2290 2318 2545 2667
Size: 28 227 122
Fragments arranged by size:
972 719 227 202 201 136 122 39
28 13 8
EcoRI G'AATT_C
Cuts at: 0 1998 2667
Size: 1998 669
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1761 2667
Size: 1761 906
FspI TGC'GCA
Cuts at: 0 862 970 2667
Size: 862 108 1697
Fragments arranged by size:
1697 862 108
HaeII r_GCGC'y
Cuts at: 0 495 1303 1422 2254 2492 2612 2667
Size: 495 808 119 832 238 120 55
Fragments arranged by size:
832 808 495 238 120 119 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 871 903 1089 1121 2020 2052 2584 2616
Size: 871 32 186 32 899 32 532 32
Cuts at: 2616 2667
Size: 51
Fragments arranged by size:
899 871 532 186 51 32 32 32
32
HincII GTy'rAC
Cuts at: 0 525 1593 2667
Size: 525 1068 1074
Fragments arranged by size:
1074 1068 525
KasI G'GCGC_C
Cuts at: 0 1299 1418 2488 2608 2667
Size: 1299 119 1070 120 59
Fragments arranged by size:
1299 1070 120 119 59
MscI TGG'CCA
Cuts at: 0 1081 1966 2667
Size: 1081 885 701
Fragments arranged by size:
1081 885 701
MslI CAynn'nnrTG
Cuts at: 0 688 898 1114 1345 1663 2667
Size: 688 210 216 231 318 1004
Fragments arranged by size:
1004 688 318 231 216 210
MspA1I CmG'CkG
Cuts at: 0 213 334 383 1480 1629 2247 2370 2503
Size: 213 121 49 1097 149 618 123 133
Cuts at: 2503 2557 2667
Size: 54 110
Fragments arranged by size:
1097 618 213 149 133 123 121 110
54 49
NaeI GCC'GGC
Cuts at: 0 220 419 1294 2616 2667
Size: 220 199 875 1322 51
Fragments arranged by size:
1322 875 220 199 51
NarI GG'CG_CC
Cuts at: 0 1300 1419 2489 2609 2667
Size: 1300 119 1070 120 58
Fragments arranged by size:
1300 1070 120 119 58
NgoMIV G'CCGG_C
Cuts at: 0 218 417 1292 2614 2667
Size: 218 199 875 1322 53
Fragments arranged by size:
1322 875 218 199 53
NotI GC'GGCC_GC
Cuts at: 0 246 2230 2667
Size: 246 1984 437
Fragments arranged by size:
1984 437 246
NspI r_CATG'y
Cuts at: 0 1275 1461 2667
Size: 1275 186 1206
Fragments arranged by size:
1275 1206 186
PciI A'CATG_T
Cuts at: 0 1457 2667
Size: 1457 1210
PflMI CCAn_nnn'nTGG
Cuts at: 0 1404 2667
Size: 1404 1263
PfoI T'CCnGG_A
Cuts at: 0 827 2072 2667
Size: 827 1245 595
Fragments arranged by size:
1245 827 595
PmlI CAC'GTG
Cuts at: 0 866 2667
Size: 866 1801
PspOMI G'GGCC_C
Cuts at: 0 135 142 337 345 358 397 447 1570
Size: 135 7 195 8 13 39 50 1123
Cuts at: 1570 2290 2317 2413 2545 2667
Size: 720 27 96 132 122
Fragments arranged by size:
1123 720 195 135 132 122 96 50
39 27 13 8 7
PstI C_TGCA'G
Cuts at: 0 515 1154 1175 2192 2667
Size: 515 639 21 1017 475
Fragments arranged by size:
1017 639 515 475 21
PvuII CAG'CTG
Cuts at: 0 1480 2667
Size: 1480 1187
RsrII CG'GwC_CG
Cuts at: 0 2583 2667
Size: 2583 84
SacI G_AGCT'C
Cuts at: 0 1565 2667
Size: 1565 1102
SacII CC_GC'GG
Cuts at: 0 214 335 384 2371 2504 2667
Size: 214 121 49 1987 133 163
Fragments arranged by size:
1987 214 163 133 121 49
SexAI A'CCwGG_T
Cuts at: 0 773 2667
Size: 773 1894
SfcI C'TryA_G
Cuts at: 0 511 1150 1171 2188 2667
Size: 511 639 21 1017 479
Fragments arranged by size:
1017 639 511 479 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 215 2667
Size: 215 2452
SfoI GGC'GCC
Cuts at: 0 1301 1420 2490 2610 2667
Size: 1301 119 1070 120 57
Fragments arranged by size:
1301 1070 120 119 57
SgrAI Cr'CCGG_yG
Cuts at: 0 2614 2667
Size: 2614 53
SmaI CCC'GGG
Cuts at: 0 30 41 134 141 169 263 1843 2288
Size: 30 11 93 7 28 94 1580 445
Cuts at: 2288 2316 2342 2362 2563 2667
Size: 28 26 20 201 104
Fragments arranged by size:
1580 445 201 104 94 93 30 28
28 26 20 11 7
SmlI C'TyrA_G
Cuts at: 0 1678 2667
Size: 1678 989
SrfI GCCC'GGGC
Cuts at: 0 41 2563 2667
Size: 41 2522 104
Fragments arranged by size:
2522 104 41
StyI C'CwwG_G
Cuts at: 0 281 362 2667
Size: 281 81 2305
Fragments arranged by size:
2305 281 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1185 1339 2667
Size: 1185 154 1328
Fragments arranged by size:
1328 1185 154
TatI w'GTAC_w
Cuts at: 0 1238 1436 1454 1742 2667
Size: 1238 198 18 288 925
Fragments arranged by size:
1238 925 288 198 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1013 1045 2667
Size: 1013 32 1622
Fragments arranged by size:
1622 1013 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1209 1974 2538 2667
Size: 1209 765 564 129
Fragments arranged by size:
1209 765 564 129
XmaI C'CCGG_G
Cuts at: 0 28 39 132 139 167 261 1841 2286
Size: 28 11 93 7 28 94 1580 445
Cuts at: 2286 2314 2340 2360 2561 2667
Size: 28 26 20 201 106
Fragments arranged by size:
1580 445 201 106 94 93 28 28
28 26 20 11 7
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AlwNI ApaI
ApoI AscI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI MscI MslI MspA1I NaeI NarI NgoMIV NotI
NspI PciI PflMI PfoI PmlI PspOMI PstI PvuII
RsrII SacI SacII SexAI SfcI SfiI SfoI SgrAI
SmaI SmlI SrfI StyI TaqII TatI TstI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AloI
ApaLI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BfrBI BmgBI BmtI BplI BsaWI BsaXI BsiWI BsmI
BspEI BspHI BsrDI BsrGI BstBI BstZ17I Bsu36I ClaI
CspCI DraI EcoRV FseI FspAI HindIII HpaI KpnI
MfeI MluI MmeI NcoI NdeI NheI NruI NsiI
PacI PasI PmeI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI SalI SanDI SapI SbfI ScaI SgfI
SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI
XhoI XmnI ZraI