Restriction map for
pF1KB7296
(Linear) (Six-base) MAPSORT of: KIBB7296.seq Check: 9186 from: 1 to: 3999
KIBB7296.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
Acc65I G'GTAC_C
Cuts at: 0 3142 3999
Size: 3142 857
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 185 1613 1766 3999
Size: 118 67 1428 153 2233
Fragments arranged by size:
2233 1428 153 118 67
AflIII A'CryG_T
Cuts at: 0 216 1351 1353 3999
Size: 216 1135 2 2646
Fragments arranged by size:
2646 1135 216 2
AlwNI CAG_nnn'CTG
Cuts at: 0 15 1738 1813 1957 1972 3232 3481 3999
Size: 15 1723 75 144 15 1260 249 518
Fragments arranged by size:
1723 1260 518 249 144 75 15 15
ApoI r'AATT_y
Cuts at: 0 326 632 1327 1790 1946 2146 2529 2634
Size: 326 306 695 463 156 200 383 105
Cuts at: 2634 2696 2792 3010 3240 3999
Size: 62 96 218 230 759
Fragments arranged by size:
759 695 463 383 326 306 230 218
200 156 105 96 62
AseI AT'TA_AT
Cuts at: 0 151 548 1502 2765 3999
Size: 151 397 954 1263 1234
Fragments arranged by size:
1263 1234 954 397 151
AvaI C'yCGr_G
Cuts at: 0 112 896 3999
Size: 112 784 3103
Fragments arranged by size:
3103 784 112
AvrII C'CTAG_G
Cuts at: 0 351 3550 3999
Size: 351 3199 449
Fragments arranged by size:
3199 449 351
BamHI G'GATC_C
Cuts at: 0 3200 3999
Size: 3200 799
BanI G'GyrC_C
Cuts at: 0 69 3142 3999
Size: 69 3073 857
Fragments arranged by size:
3073 857 69
BanII G_rGCy'C
Cuts at: 0 686 3334 3999
Size: 686 2648 665
Fragments arranged by size:
2648 686 665
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 1109 1200 2441 3474 3999
Size: 857 252 91 1241 1033 525
Fragments arranged by size:
1241 1033 857 525 252 91
BfrBI ATG'CAT
Cuts at: 0 2122 3999
Size: 2122 1877
BglI GCCn_nnn'nGGC
Cuts at: 0 54 3999
Size: 54 3945
BglII A'GATC_T
Cuts at: 0 2379 3391 3999
Size: 2379 1012 608
Fragments arranged by size:
2379 1012 608
BlpI GC'TnA_GC
Cuts at: 0 185 3999
Size: 185 3814
Bme1580I G_kGCm'C
Cuts at: 0 2309 3999
Size: 2309 1690
BmrI ACTGGGnnnn_n'
Cuts at: 0 310 787 2094 3999
Size: 310 477 1307 1905
Fragments arranged by size:
1905 1307 477 310
BmtI G_CTAG'C
Cuts at: 0 1451 3999
Size: 1451 2548
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1758 2288 3999
Size: 1758 530 1711
Fragments arranged by size:
1758 1711 530
Bpu10I CC'TnA_GC
Cuts at: 0 1958 3062 3999
Size: 1958 1104 937
Fragments arranged by size:
1958 1104 937
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1895 2028 2635 3327 3663 3999
Size: 1895 133 607 692 336 336
Fragments arranged by size:
1895 692 607 336 336 133
BsaAI yAC'GTr
Cuts at: 0 1354 1841 3999
Size: 1354 487 2158
Fragments arranged by size:
2158 1354 487
BsaBI GATnn'nnATC
Cuts at: 0 2123 3320 3999
Size: 2123 1197 679
Fragments arranged by size:
2123 1197 679
BsaWI w'CCGG_w
Cuts at: 0 1637 3599 3999
Size: 1637 1962 400
Fragments arranged by size:
1962 1637 400
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3680 3710 3759 3777 3789 3807 3999
Size: 3680 30 49 18 12 18 192
Fragments arranged by size:
3680 192 49 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1705 3440 3767 3770 3785 3999
Size: 1705 1735 327 3 15 214
Fragments arranged by size:
1735 1705 327 214 15 3
BseYI C'CCAG_C
Cuts at: 0 3486 3537 3862 3999
Size: 3486 51 325 137
Fragments arranged by size:
3486 325 137 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2033 3218 3999
Size: 2033 1185 781
Fragments arranged by size:
2033 1185 781
BsiHKAI G_wGCw'C
Cuts at: 0 2096 3941 3999
Size: 2096 1845 58
Fragments arranged by size:
2096 1845 58
BsmI GAATG_Cn'
Cuts at: 0 3990 3999
Size: 3990 9
Bsp1286I G_dGCh'C
Cuts at: 0 686 2096 2309 3334 3941 3999
Size: 686 1410 213 1025 607 58
Fragments arranged by size:
1410 1025 686 607 213 58
BspHI T'CATG_A
Cuts at: 0 921 2712 3999
Size: 921 1791 1287
Fragments arranged by size:
1791 1287 921
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2333 3999
Size: 2333 1666
BsrDI GCAATG_nn'
Cuts at: 0 500 1458 2180 3354 3741 3857 3999
Size: 500 958 722 1174 387 116 142
Fragments arranged by size:
1174 958 722 500 387 142 116
BsrGI T'GTAC_A
Cuts at: 0 2394 3999
Size: 2394 1605
BssSI C'ACGA_G
Cuts at: 0 3647 3999
Size: 3647 352
BstAPI GCAn_nnn'nTGC
Cuts at: 0 15 499 3999
Size: 15 484 3500
Fragments arranged by size:
3500 484 15
BstYI r'GATC_y
Cuts at: 0 1857 1894 2379 2909 3200 3391 3999
Size: 1857 37 485 530 291 191 608
Fragments arranged by size:
1857 608 530 485 291 191 37
Bsu36I CC'TnA_GG
Cuts at: 0 585 2834 3999
Size: 585 2249 1165
Fragments arranged by size:
2249 1165 585
BtgI C'CryG_G
Cuts at: 0 3381 3729 3999
Size: 3381 348 270
Fragments arranged by size:
3381 348 270
BtsI GCAGTG_nn'
Cuts at: 0 3312 3999
Size: 3312 687
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3959 3994 3999
Size: 3959 35 5
Fragments arranged by size:
3959 35 5
DraI TTT'AAA
Cuts at: 0 425 1118 1553 3999
Size: 425 693 435 2446
Fragments arranged by size:
2446 693 435 425
DraIII CAC_nnn'GTG
Cuts at: 0 2498 2776 3999
Size: 2498 278 1223
Fragments arranged by size:
2498 1223 278
EarI CTCTTCn'nnn_
Cuts at: 0 32 137 566 1589 1676 1742 1760 3999
Size: 32 105 429 1023 87 66 18 2239
Fragments arranged by size:
2239 1023 429 105 87 66 32 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3257 3999
Size: 3257 742
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 185 1613 1766 3999
Size: 118 67 1428 153 2233
Fragments arranged by size:
2233 1428 153 118 67
EcoO109I rG'GnC_Cy
Cuts at: 0 348 2838 3999
Size: 348 2490 1161
Fragments arranged by size:
2490 1161 348
EcoRI G'AATT_C
Cuts at: 0 1790 2634 3999
Size: 1790 844 1365
Fragments arranged by size:
1790 1365 844
EcoRV GAT'ATC
Cuts at: 0 1625 2958 3999
Size: 1625 1333 1041
Fragments arranged by size:
1625 1333 1041
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 320 1363 3413 3865 3999
Size: 320 1043 2050 452 134
Fragments arranged by size:
2050 1043 452 320 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 2712 2744 3589 3621 3661 3693
Size: 256 32 2424 32 845 32 40 32
Cuts at: 3693 3999
Size: 306
Fragments arranged by size:
2424 845 306 256 40 32 32 32
32
HincII GTy'rAC
Cuts at: 0 3260 3999
Size: 3260 739
HpaI GTT'AAC
Cuts at: 0 3260 3999
Size: 3260 739
KpnI G_GTAC'C
Cuts at: 0 3146 3999
Size: 3146 853
MfeI C'AATT_G
Cuts at: 0 242 1605 3999
Size: 242 1363 2394
Fragments arranged by size:
2394 1363 242
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2394 3723 3959 3999
Size: 2394 1329 236 40
Fragments arranged by size:
2394 1329 236 40
MslI CAynn'nnrTG
Cuts at: 0 533 2102 3734 3962 3999
Size: 533 1569 1632 228 37
Fragments arranged by size:
1632 1569 533 228 37
MspA1I CmG'CkG
Cuts at: 0 15 1591 3999
Size: 15 1576 2408
Fragments arranged by size:
2408 1576 15
NcoI C'CATG_G
Cuts at: 0 3381 3729 3999
Size: 3381 348 270
Fragments arranged by size:
3381 348 270
NdeI CA'TA_TG
Cuts at: 0 882 1847 3999
Size: 882 965 2152
Fragments arranged by size:
2152 965 882
NheI G'CTAG_C
Cuts at: 0 1447 3999
Size: 1447 2552
NsiI A_TGCA'T
Cuts at: 0 2124 3999
Size: 2124 1875
NspI r_CATG'y
Cuts at: 0 1214 1537 1776 3999
Size: 1214 323 239 2223
Fragments arranged by size:
2223 1214 323 239
PmlI CAC'GTG
Cuts at: 0 1354 3999
Size: 1354 2645
PpuMI rG'GwC_Cy
Cuts at: 0 348 2838 3999
Size: 348 2490 1161
Fragments arranged by size:
2490 1161 348
PsiI TTA'TAA
Cuts at: 0 359 667 1333 1930 2866 3999
Size: 359 308 666 597 936 1133
Fragments arranged by size:
1133 936 666 597 359 308
PstI C_TGCA'G
Cuts at: 0 417 3170 3999
Size: 417 2753 829
Fragments arranged by size:
2753 829 417
PvuII CAG'CTG
Cuts at: 0 15 1591 3999
Size: 15 1576 2408
Fragments arranged by size:
2408 1576 15
SapI GCTCTTCn'nnn_
Cuts at: 0 137 566 3999
Size: 137 429 3433
Fragments arranged by size:
3433 429 137
SexAI A'CCwGG_T
Cuts at: 0 371 912 2241 3138 3999
Size: 371 541 1329 897 861
Fragments arranged by size:
1329 897 861 541 371
SfcI C'TryA_G
Cuts at: 0 413 1675 1985 3166 3999
Size: 413 1262 310 1181 833
Fragments arranged by size:
1262 1181 833 413 310
SmlI C'TyrA_G
Cuts at: 0 112 896 1910 2007 2614 3342 3678 3999
Size: 112 784 1014 97 607 728 336 321
Fragments arranged by size:
1014 784 728 607 336 321 112 97
SnaBI TAC'GTA
Cuts at: 0 1841 3999
Size: 1841 2158
SpeI A'CTAG_T
Cuts at: 0 831 3999
Size: 831 3168
SphI G_CATG'C
Cuts at: 0 1537 3999
Size: 1537 2462
SspI AAT'ATT
Cuts at: 0 1284 1755 2253 2743 3999
Size: 1284 471 498 490 1256
Fragments arranged by size:
1284 1256 498 490 471
StyI C'CwwG_G
Cuts at: 0 351 1781 3178 3381 3550 3729 3999
Size: 351 1430 1397 203 169 179 270
Fragments arranged by size:
1430 1397 351 270 203 179 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2354 3962 3999
Size: 2354 1608 37
Fragments arranged by size:
2354 1608 37
TatI w'GTAC_w
Cuts at: 0 1642 2394 3223 3999
Size: 1642 752 829 776
Fragments arranged by size:
1642 829 776 752
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 53 85 3680 3712 3999
Size: 53 32 3595 32 287
Fragments arranged by size:
3595 287 53 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2243 3999
Size: 2243 1756
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 248 1026 3999
Size: 248 778 2973
Fragments arranged by size:
2973 778 248
XhoI C'TCGA_G
Cuts at: 0 112 896 3999
Size: 112 784 3103
Fragments arranged by size:
3103 784 112
XmnI GAAnn'nnTTC
Cuts at: 0 198 218 326 1001 3999
Size: 198 20 108 675 2998
Fragments arranged by size:
2998 675 198 108 20
Enzymes that do cut:
Acc65I AcuI AflIII AlwNI ApoI AseI AvaI AvrII
BamHI BanI BanII BbsI BfrBI BglI BglII BlpI
Bme1580I BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstYI Bsu36I
BtgI BtsI CspCI DraI DraIII EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I HincII HpaI KpnI
MfeI MmeI MslI MspA1I NcoI NdeI NheI NsiI
NspI PmlI PpuMI PsiI PstI PvuII SapI SexAI
SfcI SmlI SnaBI SpeI SphI SspI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI BaeI
BbeI BbvCI BcgI BciVI BclI BmgBI BpmI BsaI
BsaHI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BstBI BstEII BstXI BstZ17I BtgZI ClaI DrdI EaeI
EagI EcoICRI EcoNI FseI FspI FspAI HaeII HindIII
KasI MluI MscI NaeI NarI NgoMIV NotI NruI
PacI PasI PciI PflMI PfoI PmeI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SrfI StuI SwaI XbaI XmaI ZraI