Restriction map for
pF1KB7303
(Linear) (Six-base) MAPSORT of: KIBB7303.seq Check: 4840 from: 1 to: 3864
KIBB7303.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 597 780 1745 2492 3864
Size: 597 183 965 747 1372
Fragments arranged by size:
1372 965 747 597 183
AatII G_ACGT'C
Cuts at: 0 62 3700 3864
Size: 62 3638 164
Fragments arranged by size:
3638 164 62
Acc65I G'GTAC_C
Cuts at: 0 46 3461 3864
Size: 46 3415 403
Fragments arranged by size:
3415 403 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 822 1068 3021 3864
Size: 822 246 1953 843
Fragments arranged by size:
1953 843 822 246
AfeI AGC'GCT
Cuts at: 0 739 3864
Size: 739 3125
AflIII A'CryG_T
Cuts at: 0 301 414 584 644 2897 3864
Size: 301 113 170 60 2253 967
Fragments arranged by size:
2253 967 301 170 113 60
AgeI A'CCGG_T
Cuts at: 0 2508 3864
Size: 2508 1356
AhdI GACnn_n'nnGTC
Cuts at: 0 2757 3864
Size: 2757 1107
AleI CACnn'nnGTG
Cuts at: 0 244 643 961 2378 2509 3864
Size: 244 399 318 1417 131 1355
Fragments arranged by size:
1417 1355 399 318 244 131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 1505 2396 2500 3421 3864
Size: 202 1303 891 104 921 443
Fragments arranged by size:
1303 921 891 443 202 104
AlwNI CAG_nnn'CTG
Cuts at: 0 204 1650 2418 3864
Size: 204 1446 768 1446
Fragments arranged by size:
1446 1446 768 204
ApaI G_GGCC'C
Cuts at: 0 513 594 868 1112 1162 1325 1506 2072
Size: 513 81 274 244 50 163 181 566
Cuts at: 2072 2191 2333 3852 3864
Size: 119 142 1519 12
Fragments arranged by size:
1519 566 513 274 244 181 163 142
119 81 50 12
ApaLI G'TGCA_C
Cuts at: 0 1409 1522 2371 3406 3864
Size: 1409 113 849 1035 458
Fragments arranged by size:
1409 1035 849 458 113
ApoI r'AATT_y
Cuts at: 0 982 3864
Size: 982 2882
AvaI C'yCGr_G
Cuts at: 0 10 191 542 623 689 861 1111 1499
Size: 10 181 351 81 66 172 250 388
Cuts at: 1499 1936 1970 2930 3185 3211 3501 3715 3817
Size: 437 34 960 255 26 290 214 102
Cuts at: 3817 3864
Size: 47
Fragments arranged by size:
960 437 388 351 290 255 250 214
181 172 102 81 66 47 34 26
10
BanI G'GyrC_C
Cuts at: 0 46 238 331 442 822 1912 2380 2561
Size: 46 192 93 111 380 1090 468 181
Cuts at: 2561 2632 2706 3162 3461 3634 3864
Size: 71 74 456 299 173 230
Fragments arranged by size:
1090 468 456 380 299 230 192 181
173 111 93 74 71 46
BanII G_rGCy'C
Cuts at: 0 513 594 868 1112 1128 1162 1325 1506
Size: 513 81 274 244 16 34 163 181
Cuts at: 1506 2072 2191 2333 2819 3502 3617 3852 3864
Size: 566 119 142 486 683 115 235 12
Fragments arranged by size:
683 566 513 486 274 244 235 181
163 142 119 115 81 34 16 12
BbeI G_GCGC'C
Cuts at: 0 826 2710 3166 3638 3864
Size: 826 1884 456 472 226
Fragments arranged by size:
1884 826 472 456 226
BbsI GAAGACnn'nnnn_
Cuts at: 0 987 1921 2664 2966 3864
Size: 987 934 743 302 898
Fragments arranged by size:
987 934 898 743 302
BbvCI CC'TCA_GC
Cuts at: 0 1440 3864
Size: 1440 2424
BfrBI ATG'CAT
Cuts at: 0 1486 3864
Size: 1486 2378
BglI GCCn_nnn'nGGC
Cuts at: 0 14 872 1282 1387 2704 2779 3283 3612
Size: 14 858 410 105 1317 75 504 329
Cuts at: 3612 3856 3864
Size: 244 8
Fragments arranged by size:
1317 858 504 410 329 244 105 75
14 8
BlpI GC'TnA_GC
Cuts at: 0 1342 1651 2246 2879 3864
Size: 1342 309 595 633 985
Fragments arranged by size:
1342 985 633 595 309
Bme1580I G_kGCm'C
Cuts at: 0 76 86 241 371 445 513 594 698
Size: 76 10 155 130 74 68 81 104
Cuts at: 698 868 1112 1162 1325 1413 1506 1526 1753
Size: 170 244 50 163 88 93 20 227
Cuts at: 1753 1915 2057 2072 2191 2322 2333 2375 2564
Size: 162 142 15 119 131 11 42 189
Cuts at: 2564 2775 3410 3852 3864
Size: 211 635 442 12
Fragments arranged by size:
635 442 244 227 211 189 170 163
162 155 142 131 130 119 104 93
88 81 76 74 68 50 42 20
15 12 11 10
BmgBI CAC'GTC
Cuts at: 0 953 993 1578 2445 2841 3245 3864
Size: 953 40 585 867 396 404 619
Fragments arranged by size:
953 867 619 585 404 396 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 63 1983 2459 3056 3864
Size: 63 1920 476 597 808
Fragments arranged by size:
1920 808 597 476 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2970 3864
Size: 2970 894
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 195 286 421 547 831 1009 1300
Size: 184 11 91 135 126 284 178 291
Cuts at: 1300 1369 1681 2965 3472 3864
Size: 69 312 1284 507 392
Fragments arranged by size:
1284 507 392 312 291 284 184 178
135 126 91 69 11
Bpu10I CC'TnA_GC
Cuts at: 0 1440 1874 3864
Size: 1440 434 1990
Fragments arranged by size:
1990 1440 434
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 3864
Size: 763 3101
BsaI GGTCTCn'nnnn_
Cuts at: 0 308 1618 2011 3864
Size: 308 1310 393 1853
Fragments arranged by size:
1853 1310 393 308
BsaAI yAC'GTr
Cuts at: 0 87 304 587 645 744 3864
Size: 87 217 283 58 99 3120
Fragments arranged by size:
3120 283 217 99 87 58
BsaBI GATnn'nnATC
Cuts at: 0 1181 3864
Size: 1181 2683
BsaHI Gr'CG_yC
Cuts at: 0 59 451 713 823 1016 2669 2707 3163
Size: 59 392 262 110 193 1653 38 456
Cuts at: 3163 3536 3635 3697 3864
Size: 373 99 62 167
Fragments arranged by size:
1653 456 392 373 262 193 167 110
99 62 59 38
BsaWI w'CCGG_w
Cuts at: 0 2508 3864
Size: 2508 1356
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1654 1684 1950 1980 3445 3475 3864
Size: 1654 30 266 30 1465 30 389
Fragments arranged by size:
1654 1465 389 266 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1366 1678 2695 3577 3864
Size: 1366 312 1017 882 287
Fragments arranged by size:
1366 1017 882 312 287
BseYI C'CCAG_C
Cuts at: 0 593 630 976 1788 2043 2192 3382 3864
Size: 593 37 346 812 255 149 1190 482
Fragments arranged by size:
1190 812 593 482 346 255 149 37
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 409 508 766 808 1654 1717 2419
Size: 307 102 99 258 42 846 63 702
Cuts at: 2419 2731 2866 3019 3864
Size: 312 135 153 845
Fragments arranged by size:
846 845 702 312 307 258 153 135
102 99 63 42
BsiEI CG_ry'CG
Cuts at: 0 39 872 1882 1920 3672 3864
Size: 39 833 1010 38 1752 192
Fragments arranged by size:
1752 1010 833 192 39 38
BsiHKAI G_wGCw'C
Cuts at: 0 572 860 992 1413 1526 1764 2375 2819
Size: 572 288 132 421 113 238 611 444
Cuts at: 2819 3242 3410 3824 3864
Size: 423 168 414 40
Fragments arranged by size:
611 572 444 423 421 414 288 238
168 132 113 40
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2386 3346 3864
Size: 2386 960 518
Fragments arranged by size:
2386 960 518
Bsp1286I G_dGCh'C
Cuts at: 0 76 86 241 371 445 513 572 594
Size: 76 10 155 130 74 68 59 22
Cuts at: 594 698 860 868 992 1112 1128 1162 1325
Size: 104 162 8 124 120 16 34 163
Cuts at: 1325 1413 1506 1526 1753 1764 1915 2057 2072
Size: 88 93 20 227 11 151 142 15
Cuts at: 2072 2191 2322 2333 2375 2564 2775 2819 3242
Size: 119 131 11 42 189 211 44 423
Cuts at: 3242 3410 3502 3617 3824 3852 3864
Size: 168 92 115 207 28 12
Fragments arranged by size:
423 227 211 207 189 168 163 162
155 151 142 131 130 124 120 119
115 104 93 92 88 76 74 68
59 44 42 34 28 22 20 16
15 12 11 11 10 8
BspHI T'CATG_A
Cuts at: 0 2234 3864
Size: 2234 1630
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 326 485 597 780 1745 2039 2492 2753
Size: 326 159 112 183 965 294 453 261
Cuts at: 2753 3659 3864
Size: 906 205
Fragments arranged by size:
965 906 453 326 294 261 205 183
159 112
BsrBI CCG'CTC
Cuts at: 0 270 282 1802 1842 1878 3864
Size: 270 12 1520 40 36 1986
Fragments arranged by size:
1986 1520 270 40 36 12
BsrDI GCAATG_nn'
Cuts at: 0 2240 3864
Size: 2240 1624
BsrFI r'CCGG_y
Cuts at: 0 175 871 1397 1881 1915 2508 3458 3864
Size: 175 696 526 484 34 593 950 406
Fragments arranged by size:
950 696 593 526 484 406 175 34
BstEII G'GTnAC_C
Cuts at: 0 2130 3702 3864
Size: 2130 1572 162
Fragments arranged by size:
2130 1572 162
BstXI CCAn_nnnn'nTGG
Cuts at: 0 343 672 2106 3864
Size: 343 329 1434 1758
Fragments arranged by size:
1758 1434 343 329
BstYI r'GATC_y
Cuts at: 0 3446 3864
Size: 3446 418
Bsu36I CC'TnA_GG
Cuts at: 0 377 1259 3864
Size: 377 882 2605
Fragments arranged by size:
2605 882 377
BtgI C'CryG_G
Cuts at: 0 39 342 429 671 759 827 956 1052
Size: 39 303 87 242 88 68 129 96
Cuts at: 1052 1361 2064 2159 3079 3618 3639 3864
Size: 309 703 95 920 539 21 225
Fragments arranged by size:
920 703 539 309 303 242 225 129
96 95 88 87 68 39 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1479 3864
Size: 1479 2385
BtsI GCAGTG_nn'
Cuts at: 0 259 1675 1929 3864
Size: 259 1416 254 1935
Fragments arranged by size:
1935 1416 259 254
EaeI y'GGCC_r
Cuts at: 0 248 362 869 918 1289 1358 1379 1879
Size: 248 114 507 49 371 69 21 500
Cuts at: 1879 1917 2518 2549 2885 3604 3669 3864
Size: 38 601 31 336 719 65 195
Fragments arranged by size:
719 601 507 500 371 336 248 195
114 69 65 49 38 31 21
EagI C'GGCC_G
Cuts at: 0 869 1879 1917 3669 3864
Size: 869 1010 38 1752 195
Fragments arranged by size:
1752 1010 869 195 38
EarI CTCTTCn'nnn_
Cuts at: 0 386 1084 3864
Size: 386 698 2780
Fragments arranged by size:
2780 698 386
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 142 2977 3087 3864
Size: 142 2835 110 777
Fragments arranged by size:
2835 777 142 110
EcoICRI GAG'CTC
Cuts at: 0 2817 3864
Size: 2817 1047
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 195 286 421 547 822 831 1009
Size: 184 11 91 135 126 275 9 178
Cuts at: 1009 1068 1300 1369 1681 2965 3021 3472 3864
Size: 59 232 69 312 1284 56 451 392
Fragments arranged by size:
1284 451 392 312 275 232 184 178
135 126 91 69 59 56 11 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 166 458 1351 3013 3864
Size: 166 292 893 1662 851
Fragments arranged by size:
1662 893 851 292 166
EcoO109I rG'GnC_Cy
Cuts at: 0 114 348 474 509 510 1029 1135 1158
Size: 114 234 126 35 1 519 106 23
Cuts at: 1158 1159 1322 1503 1706 2069 2188 2697 2718
Size: 1 163 181 203 363 119 509 21
Cuts at: 2718 2934 3275 3284 3379 3762 3771 3864
Size: 216 341 9 95 383 9 93
Fragments arranged by size:
519 509 383 363 341 234 216 203
181 163 126 119 114 106 95 93
35 23 21 9 9 1 1
EcoRI G'AATT_C
Cuts at: 0 982 3864
Size: 982 2882
FspI TGC'GCA
Cuts at: 0 679 3864
Size: 679 3185
FspAI rTGC'GCAy
Cuts at: 0 679 3864
Size: 679 3185
HaeII r_GCGC'y
Cuts at: 0 292 741 826 2710 3026 3166 3571 3594
Size: 292 449 85 1884 316 140 405 23
Cuts at: 3594 3638 3864
Size: 44 226
Fragments arranged by size:
1884 449 405 316 292 226 140 85
44 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1950 1982 2445 2477 2743 2775 3179 3211
Size: 1950 32 463 32 266 32 404 32
Cuts at: 3211 3666 3698 3864
Size: 455 32 166
Fragments arranged by size:
1950 463 455 404 266 166 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1302 3864
Size: 1302 2562
KasI G'GCGC_C
Cuts at: 0 822 2706 3162 3634 3864
Size: 822 1884 456 472 230
Fragments arranged by size:
1884 822 472 456 230
KpnI G_GTAC'C
Cuts at: 0 50 3465 3864
Size: 50 3415 399
Fragments arranged by size:
3415 399 50
MluI A'CGCG_T
Cuts at: 0 414 3864
Size: 414 3450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2741 3864
Size: 2741 1123
MscI TGG'CCA
Cuts at: 0 250 920 1291 1360 1381 2551 3864
Size: 250 670 371 69 21 1170 1313
Fragments arranged by size:
1313 1170 670 371 250 69 21
MslI CAynn'nnrTG
Cuts at: 0 232 244 643 670 961 1487 2104 2158
Size: 232 12 399 27 291 526 617 54
Cuts at: 2158 2368 2378 2465 2509 3864
Size: 210 10 87 44 1355
Fragments arranged by size:
1355 617 526 399 291 232 210 87
54 44 27 12 10
MspA1I CmG'CkG
Cuts at: 0 204 842 1012 1038 1060 1341 1638 2196
Size: 204 638 170 26 22 281 297 558
Cuts at: 2196 2211 2732 2805 3041 3081 3386 3401 3641
Size: 15 521 73 236 40 305 15 240
Cuts at: 3641 3864
Size: 223
Fragments arranged by size:
638 558 521 305 297 281 240 236
223 204 170 73 40 26 22 15
15
NaeI GCC'GGC
Cuts at: 0 177 873 1399 3864
Size: 177 696 526 2465
Fragments arranged by size:
2465 696 526 177
NarI GG'CG_CC
Cuts at: 0 823 2707 3163 3635 3864
Size: 823 1884 456 472 229
Fragments arranged by size:
1884 823 472 456 229
NcoI C'CATG_G
Cuts at: 0 671 827 1361 2064 3864
Size: 671 156 534 703 1800
Fragments arranged by size:
1800 703 671 534 156
NgoMIV G'CCGG_C
Cuts at: 0 175 871 1397 3864
Size: 175 696 526 2467
Fragments arranged by size:
2467 696 526 175
NsiI A_TGCA'T
Cuts at: 0 1488 3864
Size: 1488 2376
NspI r_CATG'y
Cuts at: 0 183 1486 1490 2901 3864
Size: 183 1303 4 1411 963
Fragments arranged by size:
1411 1303 963 183 4
PasI CC'CwG_GG
Cuts at: 0 76 1506 1557 1692 3300 3324 3864
Size: 76 1430 51 135 1608 24 540
Fragments arranged by size:
1608 1430 540 135 76 51 24
PciI A'CATG_T
Cuts at: 0 2897 3864
Size: 2897 967
PflMI CCAn_nnn'nTGG
Cuts at: 0 204 1452 2808 3864
Size: 204 1248 1356 1056
Fragments arranged by size:
1356 1248 1056 204
PfoI T'CCnGG_A
Cuts at: 0 161 2675 3197 3449 3864
Size: 161 2514 522 252 415
Fragments arranged by size:
2514 522 415 252 161
PmlI CAC'GTG
Cuts at: 0 87 304 587 645 3864
Size: 87 217 283 58 3219
Fragments arranged by size:
3219 283 217 87 58
PpuMI rG'GwC_Cy
Cuts at: 0 114 348 474 1135 1706 2718 3379 3762
Size: 114 234 126 661 571 1012 661 383
Cuts at: 3762 3771 3864
Size: 9 93
Fragments arranged by size:
1012 661 661 571 383 234 126 114
93 9
PshAI GACnn'nnGTC
Cuts at: 0 212 3864
Size: 212 3652
PspOMI G'GGCC_C
Cuts at: 0 509 590 864 1108 1158 1321 1502 2068
Size: 509 81 274 244 50 163 181 566
Cuts at: 2068 2187 2329 3848 3864
Size: 119 142 1519 16
Fragments arranged by size:
1519 566 509 274 244 181 163 142
119 81 50 16
PspXI vC'TCGA_Gb
Cuts at: 0 1936 3864
Size: 1936 1928
PstI C_TGCA'G
Cuts at: 0 1688 2270 2731 3864
Size: 1688 582 461 1133
Fragments arranged by size:
1688 1133 582 461
PvuII CAG'CTG
Cuts at: 0 1012 1038 1638 2196 2211 2805 3386 3864
Size: 1012 26 600 558 15 594 581 478
Fragments arranged by size:
1012 600 594 581 558 478 26 15
SacI G_AGCT'C
Cuts at: 0 2819 3864
Size: 2819 1045
SacII CC_GC'GG
Cuts at: 0 3082 3642 3864
Size: 3082 560 222
Fragments arranged by size:
3082 560 222
SanDI GG'GwC_CC
Cuts at: 0 3379 3762 3864
Size: 3379 383 102
Fragments arranged by size:
3379 383 102
SfcI C'TryA_G
Cuts at: 0 1684 2266 2727 3864
Size: 1684 582 461 1137
Fragments arranged by size:
1684 1137 582 461
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1387 3283 3856 3864
Size: 1387 1896 573 8
Fragments arranged by size:
1896 1387 573 8
SfoI GGC'GCC
Cuts at: 0 824 2708 3164 3636 3864
Size: 824 1884 456 472 228
Fragments arranged by size:
1884 824 472 456 228
SgrAI Cr'CCGG_yG
Cuts at: 0 2508 3864
Size: 2508 1356
SmaI CCC'GGG
Cuts at: 0 193 625 1501 1972 2932 3717 3864
Size: 193 432 876 471 960 785 147
Fragments arranged by size:
960 876 785 471 432 193 147
SmlI C'TyrA_G
Cuts at: 0 542 778 1936 3864
Size: 542 236 1158 1928
Fragments arranged by size:
1928 1158 542 236
SphI G_CATG'C
Cuts at: 0 183 1486 3864
Size: 183 1303 2378
Fragments arranged by size:
2378 1303 183
SrfI GCCC'GGGC
Cuts at: 0 1501 3864
Size: 1501 2363
StuI AGG'CCT
Cuts at: 0 1264 2851 3864
Size: 1264 1587 1013
Fragments arranged by size:
1587 1264 1013
StyI C'CwwG_G
Cuts at: 0 671 827 1361 2064 3864
Size: 671 156 534 703 1800
Fragments arranged by size:
1800 703 671 534 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 349 601 1145 1802 2368 3601 3864
Size: 349 252 544 657 566 1233 263
Fragments arranged by size:
1233 657 566 544 349 263 252
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 293 325 1654 1686 3864
Size: 293 32 1329 32 2178
Fragments arranged by size:
2178 1329 293 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3535 3864
Size: 3535 329
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 231 261 913 1537 3864
Size: 231 30 652 624 2327
Fragments arranged by size:
2327 652 624 231 30
XhoI C'TCGA_G
Cuts at: 0 542 1936 3864
Size: 542 1394 1928
Fragments arranged by size:
1928 1394 542
XmaI C'CCGG_G
Cuts at: 0 191 623 1499 1970 2930 3715 3864
Size: 191 432 876 471 960 785 149
Fragments arranged by size:
960 876 785 471 432 191 149
ZraI GAC'GTC
Cuts at: 0 60 3698 3864
Size: 60 3638 166
Fragments arranged by size:
3638 166 60
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PstI PvuII SacI SacII SanDI
SfcI SfiI SfoI SgrAI SmaI SmlI SphI SrfI
StuI StyI TaqII TstI Tth111I XcmI XhoI XmaI
ZraI
Enzymes that do not cut:
AccI AclI AflII AloI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BglII BmtI BsiWI
BsmI BspEI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
ClaI CspCI DraI DraIII DrdI EcoRV FalI FseI
HindIII HpaI MfeI NdeI NheI NotI NruI PacI
PmeI PpiI PsiI PsrI PvuI RsrII SalI SapI
SbfI ScaI SexAI SgfI SnaBI SpeI SspI SwaI
TatI XbaI XmnI