Restriction map for
pF1KB7310
(Linear) (Six-base) MAPSORT of: KIBB7310.seq Check: 9916 from: 1 to: 4824
KIBB7310.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2104 2377 2526 3393 3571 3762 4824
Size: 2104 273 149 867 178 191 1062
Fragments arranged by size:
2104 1062 867 273 191 178 149
AccI GT'mk_AC
Cuts at: 0 1103 3356 4824
Size: 1103 2253 1468
Fragments arranged by size:
2253 1468 1103
Acc65I G'GTAC_C
Cuts at: 0 3052 4824
Size: 3052 1772
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 612 2491 3803 4033 4824
Size: 612 1879 1312 230 791
Fragments arranged by size:
1879 1312 791 612 230
AfeI AGC'GCT
Cuts at: 0 4072 4824
Size: 4072 752
AhdI GACnn_n'nnGTC
Cuts at: 0 2196 4824
Size: 2196 2628
AleI CACnn'nnGTG
Cuts at: 0 2020 2047 3016 4824
Size: 2020 27 969 1808
Fragments arranged by size:
2020 1808 969 27
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2065 2456 2812 2882 4824
Size: 2065 391 356 70 1942
Fragments arranged by size:
2065 1942 391 356 70
AlwNI CAG_nnn'CTG
Cuts at: 0 1004 1125 1210 1890 2392 2536 2751 2823
Size: 1004 121 85 680 502 144 215 72
Cuts at: 2823 2854 3165 3421 3621 3661 3846 4134 4824
Size: 31 311 256 200 40 185 288 690
Fragments arranged by size:
1004 690 680 502 311 288 256 215
200 185 144 121 85 72 40 31
ApaI G_GGCC'C
Cuts at: 0 648 728 1550 1708 1977 2316 2355 3101
Size: 648 80 822 158 269 339 39 746
Cuts at: 3101 3140 3412 3784 4416 4669 4824
Size: 39 272 372 632 253 155
Fragments arranged by size:
822 746 648 632 372 339 272 269
253 158 155 80 39 39
ApoI r'AATT_y
Cuts at: 0 3802 4824
Size: 3802 1022
AvaI C'yCGr_G
Cuts at: 0 1055 1262 2144 2301 2730 4148 4210 4824
Size: 1055 207 882 157 429 1418 62 614
Fragments arranged by size:
1418 1055 882 614 429 207 157 62
BamHI G'GATC_C
Cuts at: 0 1554 1779 4824
Size: 1554 225 3045
Fragments arranged by size:
3045 1554 225
BanI G'GyrC_C
Cuts at: 0 132 638 1222 1375 2116 2148 2265 2296
Size: 132 506 584 153 741 32 117 31
Cuts at: 2296 2440 2514 2866 2904 2931 3052 3583 4371
Size: 144 74 352 38 27 121 531 788
Cuts at: 4371 4624 4824
Size: 253 200
Fragments arranged by size:
788 741 584 531 506 352 253 200
153 144 132 121 117 74 38 32
31 27
BanII G_rGCy'C
Cuts at: 0 316 413 648 728 944 976 1191 1541
Size: 316 97 235 80 216 32 215 350
Cuts at: 1541 1550 1660 1708 1977 2161 2316 2355 2380
Size: 9 110 48 269 184 155 39 25
Cuts at: 2380 2839 2947 3101 3109 3140 3412 3784 4165
Size: 459 108 154 8 31 272 372 381
Cuts at: 4165 4271 4416 4669 4773 4824
Size: 106 145 253 104 51
Fragments arranged by size:
459 381 372 350 316 272 269 253
235 216 215 184 155 154 145 110
108 106 104 97 80 51 48 39
32 31 25 9 8
BbeI G_GCGC'C
Cuts at: 0 2269 4824
Size: 2269 2555
BbsI GAAGACnn'nnnn_
Cuts at: 0 4024 4251 4824
Size: 4024 227 573
Fragments arranged by size:
4024 573 227
BbvCI CC'TCA_GC
Cuts at: 0 1626 2090 3312 3758 4592 4824
Size: 1626 464 1222 446 834 232
Fragments arranged by size:
1626 1222 834 464 446 232
BglI GCCn_nnn'nGGC
Cuts at: 0 1091 1231 1545 1972 2350 3502 4480 4824
Size: 1091 140 314 427 378 1152 978 344
Fragments arranged by size:
1152 1091 978 427 378 344 314 140
BlpI GC'TnA_GC
Cuts at: 0 1044 2739 4824
Size: 1044 1695 2085
Fragments arranged by size:
2085 1695 1044
Bme1580I G_kGCm'C
Cuts at: 0 528 648 728 875 913 1550 1708 1943
Size: 528 120 80 147 38 637 158 235
Cuts at: 1943 1977 2054 2137 2254 2299 2316 2355 2445
Size: 34 77 83 117 45 17 39 90
Cuts at: 2445 2608 2871 3038 3101 3140 3227 3412 3784
Size: 163 263 167 63 39 87 185 372
Cuts at: 3784 4416 4669 4824
Size: 632 253 155
Fragments arranged by size:
637 632 528 372 263 253 235 185
167 163 158 155 147 120 117 90
87 83 80 77 63 45 39 39
38 34 17
BmgBI CAC'GTC
Cuts at: 0 4344 4824
Size: 4344 480
BmrI ACTGGGnnnn_n'
Cuts at: 0 187 507 1173 1247 1663 2147 2694 3221
Size: 187 320 666 74 416 484 547 527
Cuts at: 3221 3628 4824
Size: 407 1196
Fragments arranged by size:
1196 666 547 527 484 416 407 320
187 74
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 408 4824
Size: 408 4416
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 1175 1846 3643 3868 3878 4236 4607
Size: 314 861 671 1797 225 10 358 371
Cuts at: 4607 4824
Size: 217
Fragments arranged by size:
1797 861 671 371 358 314 225 217
10
Bpu10I CC'TnA_GC
Cuts at: 0 281 970 1535 1626 1659 2090 2379 2941
Size: 281 689 565 91 33 431 289 562
Cuts at: 2941 3312 3758 4592 4824
Size: 371 446 834 232
Fragments arranged by size:
834 689 565 562 446 431 371 289
281 232 91 33
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1378 3688 4824
Size: 1378 2310 1136
Fragments arranged by size:
2310 1378 1136
BsaI GGTCTCn'nnnn_
Cuts at: 0 1797 2147 3703 3781 4824
Size: 1797 350 1556 78 1043
Fragments arranged by size:
1797 1556 1043 350 78
BsaHI Gr'CG_yC
Cuts at: 0 2095 2266 4824
Size: 2095 171 2558
Fragments arranged by size:
2558 2095 171
BsaWI w'CCGG_w
Cuts at: 0 680 4824
Size: 680 4144
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 972 1002 1922 1952 3254 3284 3702 3732
Size: 972 30 920 30 1302 30 418 30
Cuts at: 3732 4345 4375 4824
Size: 613 30 449
Fragments arranged by size:
1302 972 920 613 449 418 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 400 1327 1348 1590 1614 1883 1927
Size: 397 3 927 21 242 24 269 44
Cuts at: 1927 2988 3300 3775 3863 4117 4371 4589 4824
Size: 1061 312 475 88 254 254 218 235
Fragments arranged by size:
1061 927 475 397 312 269 254 254
242 235 218 88 44 24 21 3
BseYI C'CCAG_C
Cuts at: 0 136 173 1732 1861 1977 2166 2247 2392
Size: 136 37 1559 129 116 189 81 145
Cuts at: 2392 2455 2498 2523 2557 2744 2870 2878 2920
Size: 63 43 25 34 187 126 8 42
Cuts at: 2920 2946 3132 3139 3233 3326 3435 3457 3506
Size: 26 186 7 94 93 109 22 49
Cuts at: 3506 3514 3808 3877 3888 4651 4684 4760 4775
Size: 8 294 69 11 763 33 76 15
Cuts at: 4775 4824
Size: 49
Fragments arranged by size:
1559 763 294 189 187 186 145 136
129 126 116 109 94 93 81 76
69 63 49 49 43 42 37 34
33 26 25 22 15 11 8 8
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 2552 2624 2737 2878 3040 3178 3272
Size: 178 2374 72 113 141 162 138 94
Cuts at: 3272 3284 3590 4824
Size: 12 306 1234
Fragments arranged by size:
2374 1234 306 178 162 141 138 113
94 72 12
BsiHKAI G_wGCw'C
Cuts at: 0 944 976 1191 2917 3109 4824
Size: 944 32 215 1726 192 1715
Fragments arranged by size:
1726 1715 944 215 192 32
BsiWI C'GTAC_G
Cuts at: 0 3092 4824
Size: 3092 1732
BsmI GAATG_Cn'
Cuts at: 0 618 3557 4824
Size: 618 2939 1267
Fragments arranged by size:
2939 1267 618
BsmBI CGTCTCn'nnnn_
Cuts at: 0 874 898 2102 4824
Size: 874 24 1204 2722
Fragments arranged by size:
2722 1204 874 24
Bsp1286I G_dGCh'C
Cuts at: 0 316 413 528 648 728 875 913 944
Size: 316 97 115 120 80 147 38 31
Cuts at: 944 976 1191 1541 1550 1660 1708 1943 1977
Size: 32 215 350 9 110 48 235 34
Cuts at: 1977 2054 2137 2161 2254 2299 2316 2355 2380
Size: 77 83 24 93 45 17 39 25
Cuts at: 2380 2445 2608 2839 2871 2917 2947 3038 3101
Size: 65 163 231 32 46 30 91 63
Cuts at: 3101 3109 3140 3227 3412 3784 4165 4271 4416
Size: 8 31 87 185 372 381 106 145
Cuts at: 4416 4669 4773 4824
Size: 253 104 51
Fragments arranged by size:
381 372 350 316 253 235 231 215
185 163 147 145 120 115 110 106
104 97 93 91 87 83 80 77
65 63 51 48 46 45 39 38
34 32 32 31 31 30 25 24
17 9 8
BspHI T'CATG_A
Cuts at: 0 243 620 4824
Size: 243 377 4204
Fragments arranged by size:
4204 377 243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 189 228 2104 2377 2526 3393 3460 3571
Size: 189 39 1876 273 149 867 67 111
Cuts at: 3571 3762 4824
Size: 191 1062
Fragments arranged by size:
1876 1062 867 273 191 189 149 111
67 39
BsrBI CCG'CTC
Cuts at: 0 285 891 1407 3765 4824
Size: 285 606 516 2358 1059
Fragments arranged by size:
2358 1059 606 516 285
BsrDI GCAATG_nn'
Cuts at: 0 1355 2339 4824
Size: 1355 984 2485
Fragments arranged by size:
2485 1355 984
BsrFI r'CCGG_y
Cuts at: 0 2812 4824
Size: 2812 2012
BssSI C'ACGA_G
Cuts at: 0 849 3102 4824
Size: 849 2253 1722
Fragments arranged by size:
2253 1722 849
BstAPI GCAn_nnn'nTGC
Cuts at: 0 238 3042 3165 4824
Size: 238 2804 123 1659
Fragments arranged by size:
2804 1659 238 123
BstBI TT'CG_AA
Cuts at: 0 4064 4824
Size: 4064 760
BstEII G'GTnAC_C
Cuts at: 0 1410 2013 4824
Size: 1410 603 2811
Fragments arranged by size:
2811 1410 603
BstXI CCAn_nnnn'nTGG
Cuts at: 0 855 1684 2928 3713 4197 4824
Size: 855 829 1244 785 484 627
Fragments arranged by size:
1244 855 829 785 627 484
BstYI r'GATC_y
Cuts at: 0 476 695 1554 1779 4529 4824
Size: 476 219 859 225 2750 295
Fragments arranged by size:
2750 859 476 295 225 219
Bsu36I CC'TnA_GG
Cuts at: 0 4100 4824
Size: 4100 724
BtgI C'CryG_G
Cuts at: 0 48 377 2009 3563 3722 3791 4190 4440
Size: 48 329 1632 1554 159 69 399 250
Cuts at: 4440 4571 4824
Size: 131 253
Fragments arranged by size:
1632 1554 399 329 253 250 159 131
69 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1833 2040 3534 4824
Size: 1833 207 1494 1290
Fragments arranged by size:
1833 1494 1290 207
BtsI GCAGTG_nn'
Cuts at: 0 911 1512 2369 2636 2821 3187 3425 3742
Size: 911 601 857 267 185 366 238 317
Cuts at: 3742 3936 4720 4824
Size: 194 784 104
Fragments arranged by size:
911 857 784 601 366 317 267 238
194 185 104
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 320 355 778 813 1368 1403 4824
Size: 320 35 423 35 555 35 3421
Fragments arranged by size:
3421 555 423 320 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 195 259 2232 4824
Size: 195 64 1973 2592
Fragments arranged by size:
2592 1973 195 64
DrdI GACnn_nn'nnGTC
Cuts at: 0 3829 4042 4824
Size: 3829 213 782
Fragments arranged by size:
3829 782 213
EaeI y'GGCC_r
Cuts at: 0 250 1232 1286 2082 2777 2814 3365 3794
Size: 250 982 54 796 695 37 551 429
Cuts at: 3794 4136 4824
Size: 342 688
Fragments arranged by size:
982 796 695 688 551 429 342 250
54 37
EarI CTCTTCn'nnn_
Cuts at: 0 4061 4171 4824
Size: 4061 110 653
Fragments arranged by size:
4061 653 110
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 356 1109 1578 1935 1943 2670 4824
Size: 356 753 469 357 8 727 2154
Fragments arranged by size:
2154 753 727 469 357 356 8
EcoICRI GAG'CTC
Cuts at: 0 942 974 1189 3107 4824
Size: 942 32 215 1918 1717
Fragments arranged by size:
1918 1717 942 215 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 612 1175 1846 2491 3643 3803 3868
Size: 314 298 563 671 645 1152 160 65
Cuts at: 3868 3878 4033 4236 4607 4824
Size: 10 155 203 371 217
Fragments arranged by size:
1152 671 645 563 371 314 298 217
203 160 155 65 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 2449 4824
Size: 532 1917 2375
Fragments arranged by size:
2375 1917 532
EcoO109I rG'GnC_Cy
Cuts at: 0 17 319 645 724 1036 1195 1446 1546
Size: 17 302 326 79 312 159 251 100
Cuts at: 1546 1547 1705 1837 1973 1974 2312 2313 2351
Size: 1 158 132 136 1 338 1 38
Cuts at: 2351 2352 2721 2763 2985 3136 3265 3339 3408
Size: 1 369 42 222 151 129 74 69
Cuts at: 3408 3525 3569 3780 3781 3843 4044 4215 4413
Size: 117 44 211 1 62 201 171 198
Cuts at: 4413 4665 4666 4744 4798 4824
Size: 252 1 78 54 26
Fragments arranged by size:
369 338 326 312 302 252 251 222
211 201 198 171 159 158 151 136
132 129 117 100 79 78 74 69
62 54 44 42 38 26 17 1
1 1 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4038 4824
Size: 4038 786
FseI GG_CCGG'CC
Cuts at: 0 2816 4824
Size: 2816 2008
HaeII r_GCGC'y
Cuts at: 0 1117 2269 3464 3776 4074 4410 4824
Size: 1117 1152 1195 312 298 336 414
Fragments arranged by size:
1195 1152 1117 414 336 312 298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 931 963 1862 1894 2182 2214 3254 3286
Size: 931 32 899 32 288 32 1040 32
Cuts at: 3286 3702 3734 4207 4239 4663 4695 4824
Size: 416 32 473 32 424 32 129
Fragments arranged by size:
1040 931 899 473 424 416 288 129
32 32 32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 2265 4824
Size: 2265 2559
KpnI G_GTAC'C
Cuts at: 0 3056 4824
Size: 3056 1768
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 487 881 1453 4824
Size: 227 260 394 572 3371
Fragments arranged by size:
3371 572 394 260 227
MscI TGG'CCA
Cuts at: 0 3367 3796 4138 4824
Size: 3367 429 342 686
Fragments arranged by size:
3367 686 429 342
MslI CAynn'nnrTG
Cuts at: 0 64 446 2020 2047 2794 3016 3562 4824
Size: 64 382 1574 27 747 222 546 1262
Fragments arranged by size:
1574 1262 747 546 382 222 64 27
MspA1I CmG'CkG
Cuts at: 0 235 1073 1251 1702 1865 2166 2551 2561
Size: 235 838 178 451 163 301 385 10
Cuts at: 2561 2748 2895 2924 3028 3143 3237 3510 3898
Size: 187 147 29 104 115 94 273 388
Cuts at: 3898 4256 4630 4810 4824
Size: 358 374 180 14
Fragments arranged by size:
838 451 388 385 374 358 301 273
235 187 180 178 163 147 115 104
94 29 14 10
NaeI GCC'GGC
Cuts at: 0 2814 4824
Size: 2814 2010
NarI GG'CG_CC
Cuts at: 0 2266 4824
Size: 2266 2558
NcoI C'CATG_G
Cuts at: 0 2009 3791 4440 4824
Size: 2009 1782 649 384
Fragments arranged by size:
2009 1782 649 384
NgoMIV G'CCGG_C
Cuts at: 0 2812 4824
Size: 2812 2012
NspI r_CATG'y
Cuts at: 0 63 305 900 2793 4824
Size: 63 242 595 1893 2031
Fragments arranged by size:
2031 1893 595 242 63
PasI CC'CwG_GG
Cuts at: 0 720 925 1543 1726 2346 2421 2445 2536
Size: 720 205 618 183 620 75 24 91
Cuts at: 2536 3048 3835 4824
Size: 512 787 989
Fragments arranged by size:
989 787 720 620 618 512 205 183
91 75 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 195 259 753 1726 2536 4824
Size: 195 64 494 973 810 2288
Fragments arranged by size:
2288 973 810 494 195 64
PfoI T'CCnGG_A
Cuts at: 0 1191 4824
Size: 1191 3633
PpuMI rG'GwC_Cy
Cuts at: 0 319 1036 1195 1446 2763 2985 3265 3569
Size: 319 717 159 251 1317 222 280 304
Cuts at: 3569 4044 4215 4798 4824
Size: 475 171 583 26
Fragments arranged by size:
1317 717 583 475 319 304 280 251
222 171 159 26
PspOMI G'GGCC_C
Cuts at: 0 644 724 1546 1704 1973 2312 2351 3097
Size: 644 80 822 158 269 339 39 746
Cuts at: 3097 3136 3408 3780 4412 4665 4824
Size: 39 272 372 632 253 159
Fragments arranged by size:
822 746 644 632 372 339 272 269
253 159 158 80 39 39
PstI C_TGCA'G
Cuts at: 0 1886 2669 3390 4130 4261 4523 4824
Size: 1886 783 721 740 131 262 301
Fragments arranged by size:
1886 783 740 721 301 262 131
PvuII CAG'CTG
Cuts at: 0 235 2748 2895 3237 3898 4256 4810 4824
Size: 235 2513 147 342 661 358 554 14
Fragments arranged by size:
2513 661 554 358 342 235 147 14
RsrII CG'GwC_CG
Cuts at: 0 683 4824
Size: 683 4141
SacI G_AGCT'C
Cuts at: 0 944 976 1191 3109 4824
Size: 944 32 215 1918 1715
Fragments arranged by size:
1918 1715 944 215 32
SapI GCTCTTCn'nnn_
Cuts at: 0 4061 4824
Size: 4061 763
SexAI A'CCwGG_T
Cuts at: 0 255 2436 4824
Size: 255 2181 2388
Fragments arranged by size:
2388 2181 255
SfcI C'TryA_G
Cuts at: 0 1117 1562 1882 2665 3110 3386 3483 3899
Size: 1117 445 320 783 445 276 97 416
Cuts at: 3899 4126 4257 4519 4824
Size: 227 131 262 305
Fragments arranged by size:
1117 783 445 445 416 320 305 276
262 227 131 97
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1972 3502 4824
Size: 1972 1530 1322
Fragments arranged by size:
1972 1530 1322
SfoI GGC'GCC
Cuts at: 0 2267 4824
Size: 2267 2557
SmaI CCC'GGG
Cuts at: 0 2303 2732 4150 4824
Size: 2303 429 1418 674
Fragments arranged by size:
2303 1418 674 429
SmlI C'TyrA_G
Cuts at: 0 1393 3667 4824
Size: 1393 2274 1157
Fragments arranged by size:
2274 1393 1157
StuI AGG'CCT
Cuts at: 0 391 4824
Size: 391 4433
StyI C'CwwG_G
Cuts at: 0 106 533 728 983 1226 1754 2009 2543
Size: 106 427 195 255 243 528 255 534
Cuts at: 2543 3404 3500 3776 3791 4440 4508 4824
Size: 861 96 276 15 649 68 316
Fragments arranged by size:
861 649 534 528 427 316 276 255
255 243 195 106 96 68 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 150 206 806 833 1429 1807 2158 2482
Size: 150 56 600 27 596 378 351 324
Cuts at: 2482 2586 2658 2934 3318 4698 4824
Size: 104 72 276 384 1380 126
Fragments arranged by size:
1380 600 596 384 378 351 324 276
150 126 104 72 56 27
TatI w'GTAC_w
Cuts at: 0 2477 4824
Size: 2477 2347
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 255 287 972 1004 1478 1510 1922 1954
Size: 255 32 685 32 474 32 412 32
Cuts at: 1954 4824
Size: 2870
Fragments arranged by size:
2870 685 474 412 255 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 513 4824
Size: 513 4311
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 800 2127 2353 2752 3561 4824
Size: 192 608 1327 226 399 809 1263
Fragments arranged by size:
1327 1263 809 608 399 226 192
XmaI C'CCGG_G
Cuts at: 0 2301 2730 4148 4824
Size: 2301 429 1418 676
Fragments arranged by size:
2301 1418 676 429
XmnI GAAnn'nnTTC
Cuts at: 0 616 4824
Size: 616 4208
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AhdI AleI AlfI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII RsrII SacI
SapI SexAI SfcI SfiI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI
BglII BmtI BsaAI BsaBI BsiEI BspEI BsrGI BssHII
BstZ17I ClaI DraI EagI EcoRI EcoRV FspI FspAI
HincII HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI SacII SalI SanDI SbfI
ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI ZraI