Restriction map for
pF1KB7320
(Linear) (Six-base) MAPSORT of: KIBB7320.seq Check: 3627 from: 1 to: 3864
KIBB7320.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 0 1290 3491 3864
Size: 1290 2201 373
Fragments arranged by size:
2201 1290 373
AclI AA'CG_TT
Cuts at: 0 2585 3864
Size: 2585 1279
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 2336 3533 3864
Size: 1405 931 1197 331
Fragments arranged by size:
1405 1197 931 331
AfeI AGC'GCT
Cuts at: 0 319 3864
Size: 319 3545
AleI CACnn'nnGTG
Cuts at: 0 596 3864
Size: 596 3268
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 262 294 3864
Size: 262 32 3570
Fragments arranged by size:
3570 262 32
AlwNI CAG_nnn'CTG
Cuts at: 0 142 1591 2551 3864
Size: 142 1449 960 1313
Fragments arranged by size:
1449 1313 960 142
ApoI r'AATT_y
Cuts at: 0 296 919 1028 1141 1165 1279 2048 2108
Size: 296 623 109 113 24 114 769 60
Cuts at: 2108 2458 3400 3791 3864
Size: 350 942 391 73
Fragments arranged by size:
942 769 623 391 350 296 114 113
109 73 60 24
AseI AT'TA_AT
Cuts at: 0 433 1052 1316 3864
Size: 433 619 264 2548
Fragments arranged by size:
2548 619 433 264
AvaI C'yCGr_G
Cuts at: 0 1345 3481 3864
Size: 1345 2136 383
Fragments arranged by size:
2136 1345 383
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2132 2165 3864
Size: 2132 33 1699
Fragments arranged by size:
2132 1699 33
BamHI G'GATC_C
Cuts at: 0 3501 3864
Size: 3501 363
BanI G'GyrC_C
Cuts at: 0 13 73 3271 3864
Size: 13 60 3198 593
Fragments arranged by size:
3198 593 60 13
BanII G_rGCy'C
Cuts at: 0 124 237 1765 3864
Size: 124 113 1528 2099
Fragments arranged by size:
2099 1528 124 113
BbeI G_GCGC'C
Cuts at: 0 77 3864
Size: 77 3787
BbvCI CC'TCA_GC
Cuts at: 0 2645 3864
Size: 2645 1219
BciVI GTATCCnnnnn_n'
Cuts at: 0 564 3864
Size: 564 3300
BglII A'GATC_T
Cuts at: 0 412 3375 3560 3864
Size: 412 2963 185 304
Fragments arranged by size:
2963 412 304 185
BmgBI CAC'GTC
Cuts at: 0 1890 3864
Size: 1890 1974
BmrI ACTGGGnnnn_n'
Cuts at: 0 1500 3864
Size: 1500 2364
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 251 1261 3864
Size: 194 57 1010 2603
Fragments arranged by size:
2603 1010 194 57
Bpu10I CC'TnA_GC
Cuts at: 0 95 1568 2645 3864
Size: 95 1473 1077 1219
Fragments arranged by size:
1473 1219 1077 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2680 2914 3864
Size: 2680 234 950
Fragments arranged by size:
2680 950 234
BsaBI GATnn'nnATC
Cuts at: 0 1523 3864
Size: 1523 2341
BsaHI Gr'CG_yC
Cuts at: 0 74 3473 3864
Size: 74 3399 391
Fragments arranged by size:
3399 391 74
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 188 227 857 1165 2060 3593 3864
Size: 188 39 630 308 895 1533 271
Fragments arranged by size:
1533 895 630 308 271 188 39
BseYI C'CCAG_C
Cuts at: 0 17 123 286 3864
Size: 17 106 163 3578
Fragments arranged by size:
3578 163 106 17
BsiEI CG_ry'CG
Cuts at: 0 111 3864
Size: 111 3753
BsiHKAI G_wGCw'C
Cuts at: 0 237 402 1765 3864
Size: 237 165 1363 2099
Fragments arranged by size:
2099 1363 237 165
BsmI GAATG_Cn'
Cuts at: 0 191 3864
Size: 191 3673
Bsp1286I G_dGCh'C
Cuts at: 0 124 237 402 1765 3864
Size: 124 113 165 1363 2099
Fragments arranged by size:
2099 1363 165 124 113
BspHI T'CATG_A
Cuts at: 0 1032 1728 3249 3864
Size: 1032 696 1521 615
Fragments arranged by size:
1521 1032 696 615
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1302 3400 3864
Size: 1302 2098 464
Fragments arranged by size:
2098 1302 464
BsrDI GCAATG_nn'
Cuts at: 0 221 1778 2375 3071 3864
Size: 221 1557 597 696 793
Fragments arranged by size:
1557 793 696 597 221
BsrGI T'GTAC_A
Cuts at: 0 472 3864
Size: 472 3392
BssSI C'ACGA_G
Cuts at: 0 1221 3864
Size: 1221 2643
BstAPI GCAn_nnn'nTGC
Cuts at: 0 142 2571 3864
Size: 142 2429 1293
Fragments arranged by size:
2429 1293 142
BstBI TT'CG_AA
Cuts at: 0 639 3753 3864
Size: 639 3114 111
Fragments arranged by size:
3114 639 111
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1239 3864
Size: 1239 2625
BstYI r'GATC_y
Cuts at: 0 412 2954 3183 3375 3501 3560 3864
Size: 412 2542 229 192 126 59 304
Fragments arranged by size:
2542 412 304 229 192 126 59
BstZ17I GTA'TAC
Cuts at: 0 1291 3864
Size: 1291 2573
BtgI C'CryG_G
Cuts at: 0 645 3864
Size: 645 3219
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2953 3261 3864
Size: 2953 308 603
Fragments arranged by size:
2953 603 308
BtsI GCAGTG_nn'
Cuts at: 0 26 354 1553 3127 3864
Size: 26 328 1199 1574 737
Fragments arranged by size:
1574 1199 737 328 26
DraI TTT'AAA
Cuts at: 0 1284 1716 3681 3864
Size: 1284 432 1965 183
Fragments arranged by size:
1965 1284 432 183
EaeI y'GGCC_r
Cuts at: 0 108 3864
Size: 108 3756
EagI C'GGCC_G
Cuts at: 0 108 3864
Size: 108 3756
EarI CTCTTCn'nnn_
Cuts at: 0 170 1054 2315 3023 3152 3623 3755 3864
Size: 170 884 1261 708 129 471 132 109
Fragments arranged by size:
1261 884 708 471 170 132 129 109
EcoICRI GAG'CTC
Cuts at: 0 235 1763 3864
Size: 235 1528 2101
Fragments arranged by size:
2101 1528 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 251 1261 1405 2336 3533 3864
Size: 194 57 1010 144 931 1197 331
Fragments arranged by size:
1197 1010 931 331 194 144 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1348 1402 2803 3864
Size: 1348 54 1401 1061
Fragments arranged by size:
1401 1348 1061 54
EcoO109I rG'GnC_Cy
Cuts at: 0 304 3864
Size: 304 3560
EcoRV GAT'ATC
Cuts at: 0 1521 3864
Size: 1521 2343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 906 2413 2731 3049 3864
Size: 906 1507 318 318 815
Fragments arranged by size:
1507 906 815 318 318
HaeII r_GCGC'y
Cuts at: 0 77 321 3864
Size: 77 244 3543
Fragments arranged by size:
3543 244 77
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 3186 3218 3493 3525 3864
Size: 88 32 3066 32 275 32 339
Fragments arranged by size:
3066 339 275 88 32 32 32
HindIII A'AGCT_T
Cuts at: 0 2117 3864
Size: 2117 1747
KasI G'GCGC_C
Cuts at: 0 73 3864
Size: 73 3791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 793 3532 3864
Size: 606 187 2739 332
Fragments arranged by size:
2739 606 332 187
MslI CAynn'nnrTG
Cuts at: 0 596 3864
Size: 596 3268
NarI GG'CG_CC
Cuts at: 0 74 3864
Size: 74 3790
NcoI C'CATG_G
Cuts at: 0 645 3864
Size: 645 3219
NdeI CA'TA_TG
Cuts at: 0 3369 3864
Size: 3369 495
NspI r_CATG'y
Cuts at: 0 1219 3016 3864
Size: 1219 1797 848
Fragments arranged by size:
1797 1219 848
PfoI T'CCnGG_A
Cuts at: 0 1836 3864
Size: 1836 2028
PpuMI rG'GwC_Cy
Cuts at: 0 304 3864
Size: 304 3560
PspXI vC'TCGA_Gb
Cuts at: 0 3481 3864
Size: 3481 383
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2375 2407 3864
Size: 2375 32 1457
Fragments arranged by size:
2375 1457 32
PstI C_TGCA'G
Cuts at: 0 571 1548 2937 3864
Size: 571 977 1389 927
Fragments arranged by size:
1389 977 927 571
SacI G_AGCT'C
Cuts at: 0 237 1765 3864
Size: 237 1528 2099
Fragments arranged by size:
2099 1528 237
SapI GCTCTTCn'nnn_
Cuts at: 0 1054 3023 3623 3864
Size: 1054 1969 600 241
Fragments arranged by size:
1969 1054 600 241
SfcI C'TryA_G
Cuts at: 0 567 1544 2543 2933 3137 3864
Size: 567 977 999 390 204 727
Fragments arranged by size:
999 977 727 567 390 204
SfoI GGC'GCC
Cuts at: 0 75 3864
Size: 75 3789
SmlI C'TyrA_G
Cuts at: 0 1345 2695 2893 3481 3864
Size: 1345 1350 198 588 383
Fragments arranged by size:
1350 1345 588 383 198
SspI AAT'ATT
Cuts at: 0 757 827 944 973 1986 2500 3100 3864
Size: 757 70 117 29 1013 514 600 764
Fragments arranged by size:
1013 764 757 600 514 117 70 29
StyI C'CwwG_G
Cuts at: 0 645 929 3864
Size: 645 284 2935
Fragments arranged by size:
2935 645 284
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 31 2059 3864
Size: 31 2028 1805
Fragments arranged by size:
2028 1805 31
TatI w'GTAC_w
Cuts at: 0 472 2391 2535 3864
Size: 472 1919 144 1329
Fragments arranged by size:
1919 1329 472 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3229 3261 3864
Size: 3229 32 603
Fragments arranged by size:
3229 603 32
Tth111I GACn'n_nGTC
Cuts at: 0 2818 3864
Size: 2818 1046
XhoI C'TCGA_G
Cuts at: 0 1345 3481 3864
Size: 1345 2136 383
Fragments arranged by size:
2136 1345 383
XmnI GAAnn'nnTTC
Cuts at: 0 530 1028 1190 2289 3377 3864
Size: 530 498 162 1099 1088 487
Fragments arranged by size:
1099 1088 530 498 487 162
Enzymes that do cut:
AccI AclI AcuI AfeI AleI AloI AlwNI ApoI
AseI AvaI BaeI BamHI BanI BanII BbeI BbvCI
BciVI BglII BmgBI BmrI BpmI Bpu10I BpuEI BsaBI
BsaHI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI
BstZ17I BtgI BtgZI BtsI DraI EaeI EagI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI HaeII Hin4I
HindIII KasI MmeI MslI NarI NcoI NdeI NspI
PfoI PpuMI PspXI PsrI PstI SacI SapI SfcI
SfoI SmlI SspI StyI TaqII TatI TstI Tth111I
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AflIII AgeI AhdI AlfI
ApaI ApaLI AscI AsiSI AvrII BbsI BcgI BclI
BfrBI BglI BlpI Bme1580I BmtI BplI BsaI BsaAI
BsaWI BsaXI BsgI BsiWI BsmBI BspEI BsrBI BsrFI
BssHII BstEII Bsu36I ClaI CspCI DraIII DrdI EciI
EcoRI FseI FspI FspAI HincII HpaI KpnI MfeI
MluI MscI MspA1I NaeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI XbaI XcmI
XmaI ZraI