Restriction map for
pF1KB7325
(Linear) (Six-base) MAPSORT of: KIBB7325.seq Check: 4965 from: 1 to: 4947
KIBB7325.orf
With 182 enzymes: SgfI *
March 9, 2009 15:47 ..
AccI GT'mk_AC
Cuts at: 0 887 3668 3765 4947
Size: 887 2781 97 1182
Fragments arranged by size:
2781 1182 887 97
AclI AA'CG_TT
Cuts at: 0 597 4947
Size: 597 4350
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1676 1685 2240 2666 2763 2816 3175
Size: 928 748 9 555 426 97 53 359
Cuts at: 3175 3556 4541 4555 4657 4947
Size: 381 985 14 102 290
Fragments arranged by size:
985 928 748 555 426 381 359 290
102 97 53 14 9
AfeI AGC'GCT
Cuts at: 0 2149 4947
Size: 2149 2798
AflIII A'CryG_T
Cuts at: 0 3420 4947
Size: 3420 1527
AgeI A'CCGG_T
Cuts at: 0 141 4947
Size: 141 4806
AleI CACnn'nnGTG
Cuts at: 0 1145 4348 4804 4947
Size: 1145 3203 456 143
Fragments arranged by size:
3203 1145 456 143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1374 4947
Size: 1374 3573
AlwNI CAG_nnn'CTG
Cuts at: 0 87 480 910 1227 2263 2451 2474 4467
Size: 87 393 430 317 1036 188 23 1993
Cuts at: 4467 4818 4947
Size: 351 129
Fragments arranged by size:
1993 1036 430 393 351 317 188 129
87 23
ApaI G_GGCC'C
Cuts at: 0 21 2021 4947
Size: 21 2000 2926
Fragments arranged by size:
2926 2000 21
ApaLI G'TGCA_C
Cuts at: 0 838 2383 4947
Size: 838 1545 2564
Fragments arranged by size:
2564 1545 838
ApoI r'AATT_y
Cuts at: 0 797 1036 3396 3727 3822 4718 4865 4947
Size: 797 239 2360 331 95 896 147 82
Fragments arranged by size:
2360 896 797 331 239 147 95 82
AseI AT'TA_AT
Cuts at: 0 3829 4947
Size: 3829 1118
AvaI C'yCGr_G
Cuts at: 0 22 1385 2181 4947
Size: 22 1363 796 2766
Fragments arranged by size:
2766 1363 796 22
BamHI G'GATC_C
Cuts at: 0 3356 3641 4947
Size: 3356 285 1306
Fragments arranged by size:
3356 1306 285
BanI G'GyrC_C
Cuts at: 0 1309 4947
Size: 1309 3638
BanII G_rGCy'C
Cuts at: 0 21 155 462 2021 2188 4947
Size: 21 134 307 1559 167 2759
Fragments arranged by size:
2759 1559 307 167 134 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 2223 2897 3740 4947
Size: 2223 674 843 1207
Fragments arranged by size:
2223 1207 843 674
BbvCI CC'TCA_GC
Cuts at: 0 1471 2113 4947
Size: 1471 642 2834
Fragments arranged by size:
2834 1471 642
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1186 1220 4501 4535 4947
Size: 1186 34 3281 34 412
Fragments arranged by size:
3281 1186 412 34 34
BclI T'GATC_A
Cuts at: 0 825 3935 4947
Size: 825 3110 1012
Fragments arranged by size:
3110 1012 825
BglI GCCn_nnn'nGGC
Cuts at: 0 91 1181 4947
Size: 91 1090 3766
Fragments arranged by size:
3766 1090 91
BglII A'GATC_T
Cuts at: 0 3098 3804 4947
Size: 3098 706 1143
Fragments arranged by size:
3098 1143 706
BlpI GC'TnA_GC
Cuts at: 0 587 1220 4947
Size: 587 633 3727
Fragments arranged by size:
3727 633 587
Bme1580I G_kGCm'C
Cuts at: 0 21 842 2021 2163 2387 4947
Size: 21 821 1179 142 224 2560
Fragments arranged by size:
2560 1179 821 224 142 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 1706 2232 2705 4065 4947
Size: 1706 526 473 1360 882
Fragments arranged by size:
1706 1360 882 526 473
BmtI G_CTAG'C
Cuts at: 0 4612 4947
Size: 4612 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 2172 2496 3490 4064 4453 4947
Size: 109 2063 324 994 574 389 494
Fragments arranged by size:
2063 994 574 494 389 324 109
Bpu10I CC'TnA_GC
Cuts at: 0 461 1171 1471 2113 4947
Size: 461 710 300 642 2834
Fragments arranged by size:
2834 710 642 461 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 2971 4947
Size: 167 2804 1976
Fragments arranged by size:
2804 1976 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 1158 4947
Size: 1158 3789
BsaAI yAC'GTr
Cuts at: 0 387 4947
Size: 387 4560
BsaHI Gr'CG_yC
Cuts at: 0 1446 1999 2228 4947
Size: 1446 553 229 2719
Fragments arranged by size:
2719 1446 553 229
BsaWI w'CCGG_w
Cuts at: 0 45 141 4947
Size: 45 96 4806
Fragments arranged by size:
4806 96 45
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1357 1387 4571 4601 4609 4639 4947
Size: 1357 30 3184 30 8 30 308
Fragments arranged by size:
3184 1357 308 30 30 30 8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 481 717 3034 3723 3901 4947
Size: 481 236 2317 689 178 1046
Fragments arranged by size:
2317 1046 689 481 236 178
BseYI C'CCAG_C
Cuts at: 0 4 1589 1622 4947
Size: 4 1585 33 3325
Fragments arranged by size:
3325 1585 33 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1148 1231 1840 1912 2027 3028 3982
Size: 409 739 83 609 72 115 1001 954
Cuts at: 3982 4947
Size: 965
Fragments arranged by size:
1001 965 954 739 609 409 115 83
72
BsiEI CG_ry'CG
Cuts at: 0 33 127 1984 4947
Size: 33 94 1857 2963
Fragments arranged by size:
2963 1857 94 33
BsiHKAI G_wGCw'C
Cuts at: 0 842 1142 2137 2222 2387 2425 3293 4947
Size: 842 300 995 85 165 38 868 1654
Fragments arranged by size:
1654 995 868 842 300 165 85 38
Bsp1286I G_dGCh'C
Cuts at: 0 21 155 462 842 1142 2021 2137 2163
Size: 21 134 307 380 300 879 116 26
Cuts at: 2163 2188 2222 2387 2425 3293 4947
Size: 25 34 165 38 868 1654
Fragments arranged by size:
1654 879 868 380 307 300 165 134
116 38 34 26 25 21
BspEI T'CCGG_A
Cuts at: 0 45 4947
Size: 45 4902
BspHI T'CATG_A
Cuts at: 0 435 4335 4947
Size: 435 3900 612
Fragments arranged by size:
3900 612 435
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 128 1199 3359 4067 4947
Size: 128 1071 2160 708 880
Fragments arranged by size:
2160 1071 880 708 128
BsrBI CCG'CTC
Cuts at: 0 1971 1980 4947
Size: 1971 9 2967
Fragments arranged by size:
2967 1971 9
BsrDI GCAATG_nn'
Cuts at: 0 1094 2337 4947
Size: 1094 1243 2610
Fragments arranged by size:
2610 1243 1094
BsrFI r'CCGG_y
Cuts at: 0 141 1703 1880 1983 2055 2176 4947
Size: 141 1562 177 103 72 121 2771
Fragments arranged by size:
2771 1562 177 141 121 103 72
BsrGI T'GTAC_A
Cuts at: 0 4910 4947
Size: 4910 37
BssSI C'ACGA_G
Cuts at: 0 73 1975 4947
Size: 73 1902 2972
Fragments arranged by size:
2972 1902 73
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3039 4947
Size: 3039 1908
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1122 4947
Size: 1122 3825
BstYI r'GATC_y
Cuts at: 0 2772 2840 2865 3098 3356 3641 3804 4731
Size: 2772 68 25 233 258 285 163 927
Cuts at: 4731 4947
Size: 216
Fragments arranged by size:
2772 927 285 258 233 216 163 68
25
Bsu36I CC'TnA_GG
Cuts at: 0 729 4947
Size: 729 4218
BtgI C'CryG_G
Cuts at: 0 12 2819 4575 4947
Size: 12 2807 1756 372
Fragments arranged by size:
2807 1756 372 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2207 4947
Size: 2207 2740
BtsI GCAGTG_nn'
Cuts at: 0 867 1856 1912 2708 3518 4106 4529 4713
Size: 867 989 56 796 810 588 423 184
Cuts at: 4713 4947
Size: 234
Fragments arranged by size:
989 867 810 796 588 423 234 184
56
ClaI AT'CG_AT
Cuts at: 0 3128 3447 4947
Size: 3128 319 1500
Fragments arranged by size:
3128 1500 319
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3356 3391 4095 4130 4947
Size: 3356 35 704 35 817
Fragments arranged by size:
3356 817 704 35 35
DraI TTT'AAA
Cuts at: 0 3225 3401 4947
Size: 3225 176 1546
Fragments arranged by size:
3225 1546 176
DrdI GACnn_nn'nnGTC
Cuts at: 0 1917 4947
Size: 1917 3030
EaeI y'GGCC_r
Cuts at: 0 30 124 338 1874 1981 2024 2153 4212
Size: 30 94 214 1536 107 43 129 2059
Cuts at: 4212 4947
Size: 735
Fragments arranged by size:
2059 1536 735 214 129 107 94 43
30
EagI C'GGCC_G
Cuts at: 0 30 124 1981 4947
Size: 30 94 1857 2966
Fragments arranged by size:
2966 1857 94 30
EarI CTCTTCn'nnn_
Cuts at: 0 497 545 557 2413 2782 4947
Size: 497 48 12 1856 369 2165
Fragments arranged by size:
2165 1856 497 369 48 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 928 1676 1685 2172 2240 2496 2666
Size: 109 819 748 9 487 68 256 170
Cuts at: 2666 2763 2816 3175 3490 3556 4064 4453 4541
Size: 97 53 359 315 66 508 389 88
Cuts at: 4541 4555 4657 4947
Size: 14 102 290
Fragments arranged by size:
819 748 508 487 389 359 315 290
256 170 109 102 97 88 68 66
53 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 732 2026 3886 4947
Size: 732 1294 1860 1061
Fragments arranged by size:
1860 1294 1061 732
EcoO109I rG'GnC_Cy
Cuts at: 0 17 18 84 645 939 1183 1272 2018
Size: 17 1 66 561 294 244 89 746
Cuts at: 2018 4947
Size: 2929
Fragments arranged by size:
2929 746 561 294 244 89 66 17
1
EcoRI G'AATT_C
Cuts at: 0 1036 4947
Size: 1036 3911
EcoRV GAT'ATC
Cuts at: 0 4142 4947
Size: 4142 805
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3180 3888 4947
Size: 3180 708 1059
Fragments arranged by size:
3180 1059 708
HaeII r_GCGC'y
Cuts at: 0 1347 2151 4647 4947
Size: 1347 804 2496 300
Fragments arranged by size:
2496 1347 804 300
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 810 842 1796 1828 2450 2482
Size: 619 32 159 32 954 32 622 32
Cuts at: 2482 2601 2633 2796 2828 4126 4158 4609 4641
Size: 119 32 163 32 1298 32 451 32
Cuts at: 4641 4947
Size: 306
Fragments arranged by size:
1298 954 622 619 451 306 163 159
119 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2556 3766 4947
Size: 2556 1210 1181
Fragments arranged by size:
2556 1210 1181
HindIII A'AGCT_T
Cuts at: 0 4505 4947
Size: 4505 442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1811 3876 4947
Size: 464 1347 2065 1071
Fragments arranged by size:
2065 1347 1071 464
MscI TGG'CCA
Cuts at: 0 340 2026 4214 4947
Size: 340 1686 2188 733
Fragments arranged by size:
2188 1686 733 340
MslI CAynn'nnrTG
Cuts at: 0 1067 1120 1132 1145 1192 1816 2354 2401
Size: 1067 53 12 13 47 624 538 47
Cuts at: 2401 3088 3200 4348 4770 4804 4947
Size: 687 112 1148 422 34 143
Fragments arranged by size:
1148 1067 687 624 538 422 143 112
53 47 47 34 13 12
MspA1I CmG'CkG
Cuts at: 0 1528 3323 4042 4815 4947
Size: 1528 1795 719 773 132
Fragments arranged by size:
1795 1528 773 719 132
NaeI GCC'GGC
Cuts at: 0 1882 1985 2057 4947
Size: 1882 103 72 2890
Fragments arranged by size:
2890 1882 103 72
NcoI C'CATG_G
Cuts at: 0 2819 4575 4947
Size: 2819 1756 372
Fragments arranged by size:
2819 1756 372
NgoMIV G'CCGG_C
Cuts at: 0 1880 1983 2055 4947
Size: 1880 103 72 2892
Fragments arranged by size:
2892 1880 103 72
NheI G'CTAG_C
Cuts at: 0 4608 4947
Size: 4608 339
NspI r_CATG'y
Cuts at: 0 2914 3199 4947
Size: 2914 285 1748
Fragments arranged by size:
2914 1748 285
PflMI CCAn_nnn'nTGG
Cuts at: 0 2769 3993 4947
Size: 2769 1224 954
Fragments arranged by size:
2769 1224 954
PmlI CAC'GTG
Cuts at: 0 387 4947
Size: 387 4560
PpuMI rG'GwC_Cy
Cuts at: 0 645 939 1272 4947
Size: 645 294 333 3675
Fragments arranged by size:
3675 645 333 294
PshAI GACnn'nnGTC
Cuts at: 0 4477 4947
Size: 4477 470
PsiI TTA'TAA
Cuts at: 0 1418 1615 4871 4947
Size: 1418 197 3256 76
Fragments arranged by size:
3256 1418 197 76
PspOMI G'GGCC_C
Cuts at: 0 17 2017 4947
Size: 17 2000 2930
Fragments arranged by size:
2930 2000 17
PstI C_TGCA'G
Cuts at: 0 4330 4463 4947
Size: 4330 133 484
Fragments arranged by size:
4330 484 133
PvuII CAG'CTG
Cuts at: 0 1528 4042 4815 4947
Size: 1528 2514 773 132
Fragments arranged by size:
2514 1528 773 132
RsrII CG'GwC_CG
Cuts at: 0 139 4947
Size: 139 4808
SalI G'TCGA_C
Cuts at: 0 3764 4947
Size: 3764 1183
SapI GCTCTTCn'nnn_
Cuts at: 0 497 2413 4947
Size: 497 1916 2534
Fragments arranged by size:
2534 1916 497
ScaI AGT'ACT
Cuts at: 0 2410 4324 4947
Size: 2410 1914 623
Fragments arranged by size:
2410 1914 623
SfcI C'TryA_G
Cuts at: 0 79 1329 4130 4326 4459 4917 4932 4947
Size: 79 1250 2801 196 133 458 15 15
Fragments arranged by size:
2801 1250 458 196 133 79 15 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 91 4947
Size: 91 4856
SmaI CCC'GGG
Cuts at: 0 24 2183 4947
Size: 24 2159 2764
Fragments arranged by size:
2764 2159 24
SmlI C'TyrA_G
Cuts at: 0 182 2986 4947
Size: 182 2804 1961
Fragments arranged by size:
2804 1961 182
SphI G_CATG'C
Cuts at: 0 2914 4947
Size: 2914 2033
SrfI GCCC'GGGC
Cuts at: 0 2183 4947
Size: 2183 2764
SspI AAT'ATT
Cuts at: 0 4751 4947
Size: 4751 196
StuI AGG'CCT
Cuts at: 0 188 1235 3076 4947
Size: 188 1047 1841 1871
Fragments arranged by size:
1871 1841 1047 188
StyI C'CwwG_G
Cuts at: 0 1476 2027 2736 2819 4575 4947
Size: 1476 551 709 83 1756 372
Fragments arranged by size:
1756 1476 709 551 372 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2051 2121 4947
Size: 2051 70 2826
Fragments arranged by size:
2826 2051 70
TatI w'GTAC_w
Cuts at: 0 2408 3250 4322 4455 4910 4947
Size: 2408 842 1072 133 455 37
Fragments arranged by size:
2408 1072 842 455 133 37
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 4571 4603 4947
Size: 4571 32 344
Fragments arranged by size:
4571 344 32
Tth111I GACn'n_nGTC
Cuts at: 0 2464 4947
Size: 2464 2483
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3465 4947
Size: 3465 1482
XmaI C'CCGG_G
Cuts at: 0 22 2181 4947
Size: 22 2159 2766
Fragments arranged by size:
2766 2159 22
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII
HindIII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NheI NspI PflMI PmlI PpuMI PshAI PsiI PspOMI
PstI PvuII RsrII SalI SapI ScaI SfcI SfiI
SmaI SmlI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AflII AhdI AloI AscI AsiSI
AvrII BaeI BbeI BciVI BfrBI BmgBI BplI BsaBI
BsiWI BsmI BsmBI BssHII BstBI BstEII BstZ17I DraIII
EciI EcoICRI FseI FspI FspAI HpaI KasI KpnI
MfeI MluI NarI NdeI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PpiI PspXI PsrI PvuI
SacI SacII SanDI SbfI SexAI SfoI SgfI SgrAI
SnaBI SpeI SwaI XbaI XhoI XmnI ZraI