Restriction map for
pF1KB7326
(Linear) (Six-base) MAPSORT of: KIBB7326.seq Check: 4525 from: 1 to: 3645
KIBB7326.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 256 3597 3645
Size: 256 3341 48
Fragments arranged by size:
3341 256 48
AccI GT'mk_AC
Cuts at: 0 20 3645
Size: 20 3625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3147 3645
Size: 3147 498
AflIII A'CryG_T
Cuts at: 0 1142 2250 3645
Size: 1142 1108 1395
Fragments arranged by size:
1395 1142 1108
AhdI GACnn_n'nnGTC
Cuts at: 0 1035 1146 3645
Size: 1035 111 2499
Fragments arranged by size:
2499 1035 111
AleI CACnn'nnGTG
Cuts at: 0 2905 3301 3645
Size: 2905 396 344
Fragments arranged by size:
2905 396 344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 257 436 2520 3645
Size: 257 179 2084 1125
Fragments arranged by size:
2084 1125 257 179
AlwNI CAG_nnn'CTG
Cuts at: 0 1831 2046 3645
Size: 1831 215 1599
Fragments arranged by size:
1831 1599 215
ApaLI G'TGCA_C
Cuts at: 0 1801 3645
Size: 1801 1844
ApoI r'AATT_y
Cuts at: 0 153 677 868 1489 1659 1740 3645
Size: 153 524 191 621 170 81 1905
Fragments arranged by size:
1905 621 524 191 170 153 81
AseI AT'TA_AT
Cuts at: 0 2429 3061 3645
Size: 2429 632 584
Fragments arranged by size:
2429 632 584
AvaI C'yCGr_G
Cuts at: 0 212 2586 3645
Size: 212 2374 1059
Fragments arranged by size:
2374 1059 212
BamHI G'GATC_C
Cuts at: 0 993 3645
Size: 993 2652
BanI G'GyrC_C
Cuts at: 0 315 2049 2985 3645
Size: 315 1734 936 660
Fragments arranged by size:
1734 936 660 315
BanII G_rGCy'C
Cuts at: 0 1101 3645
Size: 1101 2544
BbsI GAAGACnn'nnnn_
Cuts at: 0 3231 3645
Size: 3231 414
BbvCI CC'TCA_GC
Cuts at: 0 2225 2264 3645
Size: 2225 39 1381
Fragments arranged by size:
2225 1381 39
BciVI GTATCCnnnnn_n'
Cuts at: 0 2949 3644 3645
Size: 2949 695 1
Fragments arranged by size:
2949 695 1
BclI T'GATC_A
Cuts at: 0 1215 3645
Size: 1215 2430
BglI GCCn_nnn'nGGC
Cuts at: 0 2058 3316 3645
Size: 2058 1258 329
Fragments arranged by size:
2058 1258 329
Bme1580I G_kGCm'C
Cuts at: 0 1805 3221 3645
Size: 1805 1416 424
Fragments arranged by size:
1805 1416 424
BmgBI CAC'GTC
Cuts at: 0 2021 3645
Size: 2021 1624
BmrI ACTGGGnnnn_n'
Cuts at: 0 2148 3464 3487 3645
Size: 2148 1316 23 158
Fragments arranged by size:
2148 1316 158 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1085 1529 3645
Size: 551 534 444 2116
Fragments arranged by size:
2116 551 534 444
Bpu10I CC'TnA_GC
Cuts at: 0 290 2225 2264 3645
Size: 290 1935 39 1381
Fragments arranged by size:
1935 1381 290 39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 952 1226 3411 3645
Size: 952 274 2185 234
Fragments arranged by size:
2185 952 274 234
BsaI GGTCTCn'nnnn_
Cuts at: 0 1546 2790 3645
Size: 1546 1244 855
Fragments arranged by size:
1546 1244 855
BsaAI yAC'GTr
Cuts at: 0 309 3645
Size: 309 3336
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 409 2754 3645
Size: 409 2345 891
Fragments arranged by size:
2345 891 409
BseYI C'CCAG_C
Cuts at: 0 365 1884 1974 3645
Size: 365 1519 90 1671
Fragments arranged by size:
1671 1519 365 90
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 908 2002 3374 3645
Size: 331 577 1094 1372 271
Fragments arranged by size:
1372 1094 577 331 271
BsiHKAI G_wGCw'C
Cuts at: 0 1101 1805 2704 2902 3645
Size: 1101 704 899 198 743
Fragments arranged by size:
1101 899 743 704 198
BsmI GAATG_Cn'
Cuts at: 0 793 3139 3645
Size: 793 2346 506
Fragments arranged by size:
2346 793 506
Bsp1286I G_dGCh'C
Cuts at: 0 1101 1805 2704 2902 3221 3645
Size: 1101 704 899 198 319 424
Fragments arranged by size:
1101 899 704 424 319 198
BspHI T'CATG_A
Cuts at: 0 2919 3645
Size: 2919 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 256 3597 3645
Size: 256 3341 48
Fragments arranged by size:
3341 256 48
BsrBI CCG'CTC
Cuts at: 0 1191 3645
Size: 1191 2454
BsrDI GCAATG_nn'
Cuts at: 0 229 425 461 3575 3645
Size: 229 196 36 3114 70
Fragments arranged by size:
3114 229 196 70 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1831 3307 3589 3645
Size: 1831 1476 282 56
Fragments arranged by size:
1831 1476 282 56
BstXI CCAn_nnnn'nTGG
Cuts at: 0 373 3319 3472 3645
Size: 373 2946 153 173
Fragments arranged by size:
2946 373 173 153
BstYI r'GATC_y
Cuts at: 0 993 1994 3184 3645
Size: 993 1001 1190 461
Fragments arranged by size:
1190 1001 993 461
Bsu36I CC'TnA_GG
Cuts at: 0 2766 3645
Size: 2766 879
BtgI C'CryG_G
Cuts at: 0 121 1806 3645
Size: 121 1685 1839
Fragments arranged by size:
1839 1685 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 384 3645
Size: 384 3261
BtsI GCAGTG_nn'
Cuts at: 0 260 920 2603 2625 3113 3308 3645
Size: 260 660 1683 22 488 195 337
Fragments arranged by size:
1683 660 488 337 260 195 22
EaeI y'GGCC_r
Cuts at: 0 624 917 1040 1186 3645
Size: 624 293 123 146 2459
Fragments arranged by size:
2459 624 293 146 123
EarI CTCTTCn'nnn_
Cuts at: 0 1343 1479 2198 2337 2630 2813 2874 3645
Size: 1343 136 719 139 293 183 61 771
Fragments arranged by size:
1343 771 719 293 183 139 136 61
EcoICRI GAG'CTC
Cuts at: 0 1099 3645
Size: 1099 2546
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1085 1529 3147 3645
Size: 551 534 444 1618 498
Fragments arranged by size:
1618 551 534 498 444
EcoO109I rG'GnC_Cy
Cuts at: 0 361 1971 3645
Size: 361 1610 1674
Fragments arranged by size:
1674 1610 361
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 1021 1053 1132 1164 1175 1207
Size: 424 32 565 32 79 32 11 32
Cuts at: 1207 1863 1895 2642 2674 3645
Size: 656 32 747 32 971
Fragments arranged by size:
971 747 656 565 424 79 32 32
32 32 32 32 11
HindIII A'AGCT_T
Cuts at: 0 2690 3233 3645
Size: 2690 543 412
Fragments arranged by size:
2690 543 412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1309 2022 2347 2881 3472 3640 3645
Size: 380 929 713 325 534 591 168 5
Fragments arranged by size:
929 713 591 534 380 325 168 5
MscI TGG'CCA
Cuts at: 0 626 919 3645
Size: 626 293 2726
Fragments arranged by size:
2726 626 293
MslI CAynn'nnrTG
Cuts at: 0 1141 1155 1438 2905 3301 3470 3645
Size: 1141 14 283 1467 396 169 175
Fragments arranged by size:
1467 1141 396 283 175 169 14
MspA1I CmG'CkG
Cuts at: 0 1549 1978 2559 2968 3645
Size: 1549 429 581 409 677
Fragments arranged by size:
1549 677 581 429 409
NcoI C'CATG_G
Cuts at: 0 1806 3645
Size: 1806 1839
NdeI CA'TA_TG
Cuts at: 0 30 3029 3645
Size: 30 2999 616
Fragments arranged by size:
2999 616 30
NspI r_CATG'y
Cuts at: 0 1146 1429 2254 3310 3645
Size: 1146 283 825 1056 335
Fragments arranged by size:
1146 1056 825 335 283
PciI A'CATG_T
Cuts at: 0 1142 2250 3645
Size: 1142 1108 1395
Fragments arranged by size:
1395 1142 1108
PfoI T'CCnGG_A
Cuts at: 0 104 3645
Size: 104 3541
PmlI CAC'GTG
Cuts at: 0 309 3645
Size: 309 3336
PsiI TTA'TAA
Cuts at: 0 2171 3455 3645
Size: 2171 1284 190
Fragments arranged by size:
2171 1284 190
PspXI vC'TCGA_Gb
Cuts at: 0 212 3645
Size: 212 3433
PstI C_TGCA'G
Cuts at: 0 267 1823 2598 3108 3645
Size: 267 1556 775 510 537
Fragments arranged by size:
1556 775 537 510 267
PvuII CAG'CTG
Cuts at: 0 1549 1978 2968 3645
Size: 1549 429 990 677
Fragments arranged by size:
1549 990 677 429
SacI G_AGCT'C
Cuts at: 0 1101 3645
Size: 1101 2544
SapI GCTCTTCn'nnn_
Cuts at: 0 1343 2813 3645
Size: 1343 1470 832
Fragments arranged by size:
1470 1343 832
ScaI AGT'ACT
Cuts at: 0 747 1722 2748 3645
Size: 747 975 1026 897
Fragments arranged by size:
1026 975 897 747
SfcI C'TryA_G
Cuts at: 0 263 1092 1819 1990 2594 3104 3645
Size: 263 829 727 171 604 510 541
Fragments arranged by size:
829 727 604 541 510 263 171
SmlI C'TyrA_G
Cuts at: 0 212 967 1241 3426 3645
Size: 212 755 274 2185 219
Fragments arranged by size:
2185 755 274 219 212
SpeI A'CTAG_T
Cuts at: 0 3247 3645
Size: 3247 398
SphI G_CATG'C
Cuts at: 0 3310 3645
Size: 3310 335
SspI AAT'ATT
Cuts at: 0 981 2712 3645
Size: 981 1731 933
Fragments arranged by size:
1731 981 933
StyI C'CwwG_G
Cuts at: 0 1205 1806 3645
Size: 1205 601 1839
Fragments arranged by size:
1839 1205 601
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2814 3645
Size: 2814 831
TatI w'GTAC_w
Cuts at: 0 552 745 1720 2746 3545 3645
Size: 552 193 975 1026 799 100
Fragments arranged by size:
1026 975 799 552 193 100
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 467 499 1979 2011 3645
Size: 467 32 1480 32 1634
Fragments arranged by size:
1634 1480 467 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2022 3645
Size: 2022 1623
XbaI T'CTAG_A
Cuts at: 0 1590 2679 3645
Size: 1590 1089 966
Fragments arranged by size:
1590 1089 966
XhoI C'TCGA_G
Cuts at: 0 212 3645
Size: 212 3433
XmnI GAAnn'nnTTC
Cuts at: 0 791 1175 3645
Size: 791 384 2470
Fragments arranged by size:
2470 791 384
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AleI AlfI AlwNI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbsI
BbvCI BciVI BclI BglI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EcoICRI
Eco57MI EcoO109I Hin4I HindIII MmeI MscI MslI MspA1I
NcoI NdeI NspI PciI PfoI PmlI PsiI PspXI
PstI PvuII SacI SapI ScaI SfcI SmlI SpeI
SphI SspI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AloI ApaI
AscI AsiSI AvrII BaeI BbeI BcgI BfrBI BglII
BlpI BmtI BplI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PmeI PpiI PpuMI PshAI PspOMI PsrI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI StuI SwaI XcmI
XmaI ZraI