Restriction map for
pF1KB7328
(Linear) (Six-base) MAPSORT of: KIBB7328.seq Check: 9905 from: 1 to: 4077
KIBB7328.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AccI GT'mk_AC
Cuts at: 0 221 241 1850 2270 4077
Size: 221 20 1609 420 1807
Fragments arranged by size:
1807 1609 420 221 20
Acc65I G'GTAC_C
Cuts at: 0 2372 4077
Size: 2372 1705
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 1226 2415 3348 4034 4077
Size: 106 1120 1189 933 686 43
Fragments arranged by size:
1189 1120 933 686 106 43
AfeI AGC'GCT
Cuts at: 0 2424 4077
Size: 2424 1653
AflIII A'CryG_T
Cuts at: 0 363 2348 4077
Size: 363 1985 1729
Fragments arranged by size:
1985 1729 363
AhdI GACnn_n'nnGTC
Cuts at: 0 1887 4077
Size: 1887 2190
AleI CACnn'nnGTG
Cuts at: 0 184 3256 4077
Size: 184 3072 821
Fragments arranged by size:
3072 821 184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2719 2751 4077
Size: 2719 32 1326
Fragments arranged by size:
2719 1326 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1294 2438 2991 3687 4039 4077
Size: 1294 1144 553 696 352 38
Fragments arranged by size:
1294 1144 696 553 352 38
ApaI G_GGCC'C
Cuts at: 0 543 782 2489 4077
Size: 543 239 1707 1588
Fragments arranged by size:
1707 1588 543 239
ApoI r'AATT_y
Cuts at: 0 349 1238 2263 3247 4077
Size: 349 889 1025 984 830
Fragments arranged by size:
1025 984 889 830 349
AvaI C'yCGr_G
Cuts at: 0 3803 4077
Size: 3803 274
AvrII C'CTAG_G
Cuts at: 0 1578 4077
Size: 1578 2499
BamHI G'GATC_C
Cuts at: 0 2124 3606 3645 4077
Size: 2124 1482 39 432
Fragments arranged by size:
2124 1482 432 39
BanI G'GyrC_C
Cuts at: 0 2372 4077
Size: 2372 1705
BanII G_rGCy'C
Cuts at: 0 543 782 1561 2176 2470 2489 2686 2927
Size: 543 239 779 615 294 19 197 241
Cuts at: 2927 3810 4077
Size: 883 267
Fragments arranged by size:
883 779 615 543 294 267 241 239
197 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 94 522 1289 1948 2607 3578 4077
Size: 94 428 767 659 659 971 499
Fragments arranged by size:
971 767 659 659 499 428 94
BclI T'GATC_A
Cuts at: 0 932 1701 4077
Size: 932 769 2376
Fragments arranged by size:
2376 932 769
BglII A'GATC_T
Cuts at: 0 30 1520 2672 4077
Size: 30 1490 1152 1405
Fragments arranged by size:
1490 1405 1152 30
BlpI GC'TnA_GC
Cuts at: 0 3196 4077
Size: 3196 881
Bme1580I G_kGCm'C
Cuts at: 0 543 782 2489 4077
Size: 543 239 1707 1588
Fragments arranged by size:
1707 1588 543 239
BmgBI CAC'GTC
Cuts at: 0 1920 3969 4077
Size: 1920 2049 108
Fragments arranged by size:
2049 1920 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 4077
Size: 94 3983
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 342 480 2392 3829 4077
Size: 342 138 1912 1437 248
Fragments arranged by size:
1912 1437 342 248 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 1726 2201 2387 3709 4077
Size: 430 1296 475 186 1322 368
Fragments arranged by size:
1322 1296 475 430 368 186
Bpu10I CC'TnA_GC
Cuts at: 0 1753 1948 2285 3268 4077
Size: 1753 195 337 983 809
Fragments arranged by size:
1753 983 809 337 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2911 4077
Size: 2911 1166
BsaI GGTCTCn'nnnn_
Cuts at: 0 1766 2455 4077
Size: 1766 689 1622
Fragments arranged by size:
1766 1622 689
BsaAI yAC'GTr
Cuts at: 0 339 1908 2351 4077
Size: 339 1569 443 1726
Fragments arranged by size:
1726 1569 443 339
BsaBI GATnn'nnATC
Cuts at: 0 376 4077
Size: 376 3701
BsaHI Gr'CG_yC
Cuts at: 0 1901 2597 4077
Size: 1901 696 1480
Fragments arranged by size:
1901 1480 696
BsaWI w'CCGG_w
Cuts at: 0 575 3472 4077
Size: 575 2897 605
Fragments arranged by size:
2897 605 575
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2959 2989 4077
Size: 2959 30 1088
Fragments arranged by size:
2959 1088 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1437 1471 1942 2083 2634 2927 2967 3034
Size: 1437 34 471 141 551 293 40 67
Cuts at: 3034 3135 4077
Size: 101 942
Fragments arranged by size:
1437 942 551 471 293 141 101 67
40 34
BseYI C'CCAG_C
Cuts at: 0 79 2445 3343 4077
Size: 79 2366 898 734
Fragments arranged by size:
2366 898 734 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1471 3900 4077
Size: 1471 2429 177
Fragments arranged by size:
2429 1471 177
BsiEI CG_ry'CG
Cuts at: 0 1860 1895 4077
Size: 1860 35 2182
Fragments arranged by size:
2182 1860 35
BsiHKAI G_wGCw'C
Cuts at: 0 662 1561 2291 2927 3063 3810 4077
Size: 662 899 730 636 136 747 267
Fragments arranged by size:
899 747 730 662 636 267 136
BsiWI C'GTAC_G
Cuts at: 0 758 4077
Size: 758 3319
BsmI GAATG_Cn'
Cuts at: 0 3304 3557 4077
Size: 3304 253 520
Fragments arranged by size:
3304 520 253
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1098 3229 4077
Size: 1098 2131 848
Fragments arranged by size:
2131 1098 848
Bsp1286I G_dGCh'C
Cuts at: 0 543 662 782 1561 2176 2291 2470 2489
Size: 543 119 120 779 615 115 179 19
Cuts at: 2489 2686 2927 3063 3810 4077
Size: 197 241 136 747 267
Fragments arranged by size:
779 747 615 543 267 241 197 179
136 120 119 115 19
BspEI T'CCGG_A
Cuts at: 0 3472 4077
Size: 3472 605
BspHI T'CATG_A
Cuts at: 0 3095 3701 4077
Size: 3095 606 376
Fragments arranged by size:
3095 606 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 928 1880 2111 3672 4077
Size: 928 952 231 1561 405
Fragments arranged by size:
1561 952 928 405 231
BsrBI CCG'CTC
Cuts at: 0 1794 3200 4077
Size: 1794 1406 877
Fragments arranged by size:
1794 1406 877
BsrDI GCAATG_nn'
Cuts at: 0 1169 1205 2156 4077
Size: 1169 36 951 1921
Fragments arranged by size:
1921 1169 951 36
BsrFI r'CCGG_y
Cuts at: 0 47 183 203 420 2948 4077
Size: 47 136 20 217 2528 1129
Fragments arranged by size:
2528 1129 217 136 47 20
BsrGI T'GTAC_A
Cuts at: 0 2561 4077
Size: 2561 1516
BssSI C'ACGA_G
Cuts at: 0 2821 4077
Size: 2821 1256
BstEII G'GTnAC_C
Cuts at: 0 1971 2951 3129 4077
Size: 1971 980 178 948
Fragments arranged by size:
1971 980 948 178
BstXI CCAn_nnnn'nTGG
Cuts at: 0 135 2634 2745 4077
Size: 135 2499 111 1332
Fragments arranged by size:
2499 1332 135 111
BstYI r'GATC_y
Cuts at: 0 30 55 1520 1652 2124 2672 3606 3645
Size: 30 25 1465 132 472 548 934 39
Cuts at: 3645 4077
Size: 432
Fragments arranged by size:
1465 934 548 472 432 132 39 30
25
BtgI C'CryG_G
Cuts at: 0 1124 1895 3257 3348 4077
Size: 1124 771 1362 91 729
Fragments arranged by size:
1362 1124 771 729 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3915 4063 4077
Size: 3915 148 14
Fragments arranged by size:
3915 148 14
BtsI GCAGTG_nn'
Cuts at: 0 2564 4077
Size: 2564 1513
DraIII CAC_nnn'GTG
Cuts at: 0 342 4077
Size: 342 3735
EaeI y'GGCC_r
Cuts at: 0 383 569 839 1892 2864 4077
Size: 383 186 270 1053 972 1213
Fragments arranged by size:
1213 1053 972 383 270 186
EagI C'GGCC_G
Cuts at: 0 1892 4077
Size: 1892 2185
EarI CTCTTCn'nnn_
Cuts at: 0 290 2392 2407 3815 4077
Size: 290 2102 15 1408 262
Fragments arranged by size:
2102 1408 290 262 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 4077
Size: 66 4011
EcoICRI GAG'CTC
Cuts at: 0 1559 2925 3808 4077
Size: 1559 1366 883 269
Fragments arranged by size:
1559 1366 883 269
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 430 1226 1726 2201 2387 2415 3348
Size: 106 324 796 500 475 186 28 933
Cuts at: 3348 3709 4034 4077
Size: 361 325 43
Fragments arranged by size:
933 796 500 475 361 325 324 186
106 43 28
EcoNI CCTnn'n_nnAGG
Cuts at: 0 114 924 1744 4077
Size: 114 810 820 2333
Fragments arranged by size:
2333 820 810 114
EcoO109I rG'GnC_Cy
Cuts at: 0 13 313 461 540 778 1749 1797 2486
Size: 13 300 148 79 238 971 48 689
Cuts at: 2486 2988 3323 4077
Size: 502 335 754
Fragments arranged by size:
971 754 689 502 335 300 238 148
79 48 13
EcoRV GAT'ATC
Cuts at: 0 800 3552 4077
Size: 800 2752 525
Fragments arranged by size:
2752 800 525
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1938 4077
Size: 1938 2139
HaeII r_GCGC'y
Cuts at: 0 592 2082 2426 4077
Size: 592 1490 344 1651
Fragments arranged by size:
1651 1490 592 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 266 298 1097 1129 1873 1905 2452 2484
Size: 266 32 799 32 744 32 547 32
Cuts at: 2484 4077
Size: 1593
Fragments arranged by size:
1593 799 744 547 266 32 32 32
32
HincII GTy'rAC
Cuts at: 0 242 4077
Size: 242 3835
HindIII A'AGCT_T
Cuts at: 0 1497 4077
Size: 1497 2580
KpnI G_GTAC'C
Cuts at: 0 2376 4077
Size: 2376 1701
MluI A'CGCG_T
Cuts at: 0 363 4077
Size: 363 3714
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1880 2438 3438 4077
Size: 1880 558 1000 639
Fragments arranged by size:
1880 1000 639 558
MscI TGG'CCA
Cuts at: 0 385 841 2866 4077
Size: 385 456 2025 1211
Fragments arranged by size:
2025 1211 456 385
MslI CAynn'nnrTG
Cuts at: 0 184 403 1664 2587 2632 3008 3256 3665
Size: 184 219 1261 923 45 376 248 409
Cuts at: 3665 3715 4077
Size: 50 362
Fragments arranged by size:
1261 923 409 376 362 248 219 184
50 45
MspA1I CmG'CkG
Cuts at: 0 1593 3195 3789 4077
Size: 1593 1602 594 288
Fragments arranged by size:
1602 1593 594 288
NaeI GCC'GGC
Cuts at: 0 49 4077
Size: 49 4028
NdeI CA'TA_TG
Cuts at: 0 246 4077
Size: 246 3831
NgoMIV G'CCGG_C
Cuts at: 0 47 4077
Size: 47 4030
NspI r_CATG'y
Cuts at: 0 2548 4077
Size: 2548 1529
PasI CC'CwG_GG
Cuts at: 0 2490 4077
Size: 2490 1587
PflMI CCAn_nnn'nTGG
Cuts at: 0 3338 3523 4077
Size: 3338 185 554
Fragments arranged by size:
3338 554 185
PfoI T'CCnGG_A
Cuts at: 0 2217 2918 4077
Size: 2217 701 1159
Fragments arranged by size:
2217 1159 701
PmlI CAC'GTG
Cuts at: 0 339 1908 4077
Size: 339 1569 2169
Fragments arranged by size:
2169 1569 339
PpuMI rG'GwC_Cy
Cuts at: 0 13 313 461 1749 3323 4077
Size: 13 300 148 1288 1574 754
Fragments arranged by size:
1574 1288 754 300 148 13
PshAI GACnn'nnGTC
Cuts at: 0 1963 2537 4077
Size: 1963 574 1540
Fragments arranged by size:
1963 1540 574
PspOMI G'GGCC_C
Cuts at: 0 539 778 2485 4077
Size: 539 239 1707 1592
Fragments arranged by size:
1707 1592 539 239
PstI C_TGCA'G
Cuts at: 0 1598 2795 4077
Size: 1598 1197 1282
Fragments arranged by size:
1598 1282 1197
PvuII CAG'CTG
Cuts at: 0 1593 3195 3789 4077
Size: 1593 1602 594 288
Fragments arranged by size:
1602 1593 594 288
SacI G_AGCT'C
Cuts at: 0 1561 2927 3810 4077
Size: 1561 1366 883 267
Fragments arranged by size:
1561 1366 883 267
SalI G'TCGA_C
Cuts at: 0 240 4077
Size: 240 3837
SapI GCTCTTCn'nnn_
Cuts at: 0 3815 4077
Size: 3815 262
ScaI AGT'ACT
Cuts at: 0 3156 4077
Size: 3156 921
SfcI C'TryA_G
Cuts at: 0 1594 1984 2791 2876 4077
Size: 1594 390 807 85 1201
Fragments arranged by size:
1594 1201 807 390 85
SgrAI Cr'CCGG_yG
Cuts at: 0 183 4077
Size: 183 3894
SmlI C'TyrA_G
Cuts at: 0 2926 4077
Size: 2926 1151
StuI AGG'CCT
Cuts at: 0 325 3170 4077
Size: 325 2845 907
Fragments arranged by size:
2845 907 325
StyI C'CwwG_G
Cuts at: 0 599 1436 1578 2738 3332 4077
Size: 599 837 142 1160 594 745
Fragments arranged by size:
1160 837 745 599 594 142
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 1288 2527 2547 2980 4077
Size: 141 1147 1239 20 433 1097
Fragments arranged by size:
1239 1147 1097 433 141 20
TatI w'GTAC_w
Cuts at: 0 2561 3154 4077
Size: 2561 593 923
Fragments arranged by size:
2561 923 593
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2265 2297 2466 2493 2498 2525 2959 2991
Size: 2265 32 169 27 5 27 434 32
Cuts at: 2991 4077
Size: 1086
Fragments arranged by size:
2265 1086 434 169 32 32 27 27
5
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 646 1197 2988 3855 4077
Size: 646 551 1791 867 222
Fragments arranged by size:
1791 867 646 551 222
XmnI GAAnn'nnTTC
Cuts at: 0 3468 4077
Size: 3468 609
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AleI AloI
AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII
BbsI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII KpnI
MluI MmeI MscI MslI MspA1I NaeI NdeI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PshAI PspOMI
PstI PvuII SacI SalI SapI ScaI SfcI SgrAI
SmlI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AlfI ApaLI AscI
AseI AsiSI BaeI BbeI BbvCI BcgI BciVI BfrBI
BglI BmtI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EcoRI FseI FspI FspAI HpaI
KasI MfeI NarI NcoI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PsiI PspXI PsrI PvuI
RsrII SacII SanDI SbfI SexAI SfiI SfoI SgfI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI