Restriction map for
pF1KB7329
(Linear) (Six-base) MAPSORT of: KIBB7329.seq Check: 3863 from: 1 to: 2700
KIBB7329.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1493 2700
Size: 1493 1207
AatII G_ACGT'C
Cuts at: 0 1360 2700
Size: 1360 1340
AccI GT'mk_AC
Cuts at: 0 679 1537 2700
Size: 679 858 1163
Fragments arranged by size:
1163 858 679
Acc65I G'GTAC_C
Cuts at: 0 2517 2700
Size: 2517 183
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 258 362 1222 1264 1945 2497 2700
Size: 258 104 860 42 681 552 203
Fragments arranged by size:
860 681 552 258 203 104 42
AleI CACnn'nnGTG
Cuts at: 0 2356 2700
Size: 2356 344
AlwNI CAG_nnn'CTG
Cuts at: 0 2362 2700
Size: 2362 338
ApaI G_GGCC'C
Cuts at: 0 2590 2700
Size: 2590 110
ApoI r'AATT_y
Cuts at: 0 555 1158 1779 2700
Size: 555 603 621 921
Fragments arranged by size:
921 621 603 555
AseI AT'TA_AT
Cuts at: 0 455 2700
Size: 455 2245
AvaI C'yCGr_G
Cuts at: 0 1818 2165 2700
Size: 1818 347 535
Fragments arranged by size:
1818 535 347
BanI G'GyrC_C
Cuts at: 0 2517 2565 2700
Size: 2517 48 135
Fragments arranged by size:
2517 135 48
BanII G_rGCy'C
Cuts at: 0 1377 1669 2590 2700
Size: 1377 292 921 110
Fragments arranged by size:
1377 921 292 110
BbsI GAAGACnn'nnnn_
Cuts at: 0 188 196 1210 1360 1716 1802 2340 2700
Size: 188 8 1014 150 356 86 538 360
Fragments arranged by size:
1014 538 360 356 188 150 86 8
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1252 1286 2700
Size: 1252 34 1414
Fragments arranged by size:
1414 1252 34
BfrBI ATG'CAT
Cuts at: 0 84 839 2700
Size: 84 755 1861
Fragments arranged by size:
1861 755 84
Bme1580I G_kGCm'C
Cuts at: 0 1994 2271 2590 2700
Size: 1994 277 319 110
Fragments arranged by size:
1994 319 277 110
BmrI ACTGGGnnnn_n'
Cuts at: 0 2109 2175 2700
Size: 2109 66 525
Fragments arranged by size:
2109 525 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1477 2700
Size: 1477 1223
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 832 1928 2700
Size: 36 796 1096 772
Fragments arranged by size:
1096 796 772 36
Bpu10I CC'TnA_GC
Cuts at: 0 962 2700
Size: 962 1738
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1149 1366 2509 2700
Size: 1149 217 1143 191
Fragments arranged by size:
1149 1143 217 191
BsaI GGTCTCn'nnnn_
Cuts at: 0 1562 2220 2700
Size: 1562 658 480
Fragments arranged by size:
1562 658 480
BsaAI yAC'GTr
Cuts at: 0 1192 2700
Size: 1192 1508
BsaHI Gr'CG_yC
Cuts at: 0 539 1357 2700
Size: 539 818 1343
Fragments arranged by size:
1343 818 539
BsaWI w'CCGG_w
Cuts at: 0 1914 2700
Size: 1914 786
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1342 1372 2601 2631 2700
Size: 1342 30 1229 30 69
Fragments arranged by size:
1342 1229 69 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 254 1357 2700
Size: 254 1103 1343
Fragments arranged by size:
1343 1103 254
BseYI C'CCAG_C
Cuts at: 0 695 2620 2660 2700
Size: 695 1925 40 40
Fragments arranged by size:
1925 695 40 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1465 2471 2512 2700
Size: 1465 1006 41 188
Fragments arranged by size:
1465 1006 188 41
BsiEI CG_ry'CG
Cuts at: 0 2547 2700
Size: 2547 153
BsiHKAI G_wGCw'C
Cuts at: 0 860 2700
Size: 860 1840
BsmI GAATG_Cn'
Cuts at: 0 84 1889 2700
Size: 84 1805 811
Fragments arranged by size:
1805 811 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1384 2700
Size: 1384 1316
Bsp1286I G_dGCh'C
Cuts at: 0 860 1377 1669 1994 2271 2590 2700
Size: 860 517 292 325 277 319 110
Fragments arranged by size:
860 517 325 319 292 277 110
BspEI T'CCGG_A
Cuts at: 0 1914 2700
Size: 1914 786
BspHI T'CATG_A
Cuts at: 0 2250 2700
Size: 2250 450
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1493 1599 2700
Size: 1493 106 1101
Fragments arranged by size:
1493 1101 106
BsrBI CCG'CTC
Cuts at: 0 48 2073 2549 2700
Size: 48 2025 476 151
Fragments arranged by size:
2025 476 151 48
BsrDI GCAATG_nn'
Cuts at: 0 1005 2700
Size: 1005 1695
BsrFI r'CCGG_y
Cuts at: 0 2542 2700
Size: 2542 158
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2176 2362 2700
Size: 2176 186 338
Fragments arranged by size:
2176 338 186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2037 2391 2700
Size: 2037 354 309
Fragments arranged by size:
2037 354 309
BstYI r'GATC_y
Cuts at: 0 213 1041 1068 1457 1911 2399 2430 2438
Size: 213 828 27 389 454 488 31 8
Cuts at: 2438 2700
Size: 262
Fragments arranged by size:
828 488 454 389 262 213 31 27
8
BstZ17I GTA'TAC
Cuts at: 0 680 2700
Size: 680 2020
BtgI C'CryG_G
Cuts at: 0 774 1994 2471 2700
Size: 774 1220 477 229
Fragments arranged by size:
1220 774 477 229
BtsI GCAGTG_nn'
Cuts at: 0 1313 1556 2294 2351 2363 2569 2700
Size: 1313 243 738 57 12 206 131
Fragments arranged by size:
1313 738 243 206 131 57 12
DraI TTT'AAA
Cuts at: 0 1785 2700
Size: 1785 915
DraIII CAC_nnn'GTG
Cuts at: 0 2565 2700
Size: 2565 135
DrdI GACnn_nn'nnGTC
Cuts at: 0 2475 2700
Size: 2475 225
EaeI y'GGCC_r
Cuts at: 0 771 2333 2381 2544 2700
Size: 771 1562 48 163 156
Fragments arranged by size:
1562 771 163 156 48
EagI C'GGCC_G
Cuts at: 0 2544 2700
Size: 2544 156
EarI CTCTTCn'nnn_
Cuts at: 0 313 2700
Size: 313 2387
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2582 2700
Size: 2582 118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 258 362 832 1222 1264 1928 1945
Size: 36 222 104 470 390 42 664 17
Cuts at: 1945 2497 2700
Size: 552 203
Fragments arranged by size:
664 552 470 390 222 203 104 42
36 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2594 2700
Size: 2594 106
EcoO109I rG'GnC_Cy
Cuts at: 0 2586 2587 2645 2700
Size: 2586 1 58 55
Fragments arranged by size:
2586 58 55 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1647 2700
Size: 1647 1053
FspI TGC'GCA
Cuts at: 0 1888 2700
Size: 1888 812
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 205 237 283 315 1219 1251 1342 1374
Size: 205 32 46 32 904 32 91 32
Cuts at: 1374 2232 2264 2700
Size: 858 32 436
Fragments arranged by size:
904 858 436 205 91 46 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1628 2700
Size: 1628 1072
KpnI G_GTAC'C
Cuts at: 0 2521 2700
Size: 2521 179
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 2700
Size: 991 1709
MscI TGG'CCA
Cuts at: 0 773 2335 2700
Size: 773 1562 365
Fragments arranged by size:
1562 773 365
MslI CAynn'nnrTG
Cuts at: 0 89 590 608 1330 2249 2356 2700
Size: 89 501 18 722 919 107 344
Fragments arranged by size:
919 722 501 344 107 89 18
MspA1I CmG'CkG
Cuts at: 0 297 630 2314 2700
Size: 297 333 1684 386
Fragments arranged by size:
1684 386 333 297
NaeI GCC'GGC
Cuts at: 0 2544 2700
Size: 2544 156
NcoI C'CATG_G
Cuts at: 0 774 2471 2700
Size: 774 1697 229
Fragments arranged by size:
1697 774 229
NdeI CA'TA_TG
Cuts at: 0 1152 2700
Size: 1152 1548
NgoMIV G'CCGG_C
Cuts at: 0 2542 2700
Size: 2542 158
NsiI A_TGCA'T
Cuts at: 0 86 841 2700
Size: 86 755 1859
Fragments arranged by size:
1859 755 86
NspI r_CATG'y
Cuts at: 0 1021 2700
Size: 1021 1679
PasI CC'CwG_GG
Cuts at: 0 2641 2700
Size: 2641 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 2121 2700
Size: 2121 579
PfoI T'CCnGG_A
Cuts at: 0 1934 2441 2700
Size: 1934 507 259
Fragments arranged by size:
1934 507 259
PpuMI rG'GwC_Cy
Cuts at: 0 2645 2700
Size: 2645 55
PsiI TTA'TAA
Cuts at: 0 1432 2700
Size: 1432 1268
PspOMI G'GGCC_C
Cuts at: 0 2586 2700
Size: 2586 114
PstI C_TGCA'G
Cuts at: 0 989 2358 2510 2576 2700
Size: 989 1369 152 66 124
Fragments arranged by size:
1369 989 152 124 66
PvuII CAG'CTG
Cuts at: 0 297 630 2700
Size: 297 333 2070
Fragments arranged by size:
2070 333 297
SanDI GG'GwC_CC
Cuts at: 0 2645 2700
Size: 2645 55
SapI GCTCTTCn'nnn_
Cuts at: 0 313 2700
Size: 313 2387
SexAI A'CCwGG_T
Cuts at: 0 2561 2700
Size: 2561 139
SfcI C'TryA_G
Cuts at: 0 385 985 1212 2354 2506 2572 2700
Size: 385 600 227 1142 152 66 128
Fragments arranged by size:
1142 600 385 227 152 128 66
SmlI C'TyrA_G
Cuts at: 0 1164 1381 2524 2700
Size: 1164 217 1143 176
Fragments arranged by size:
1164 1143 217 176
SspI AAT'ATT
Cuts at: 0 1173 2700
Size: 1173 1527
StuI AGG'CCT
Cuts at: 0 2581 2700
Size: 2581 119
StyI C'CwwG_G
Cuts at: 0 774 1737 2088 2236 2471 2480 2700
Size: 774 963 351 148 235 9 220
Fragments arranged by size:
963 774 351 235 220 148 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 709 1769 2674 2700
Size: 709 1060 905 26
Fragments arranged by size:
1060 905 709 26
TatI w'GTAC_w
Cuts at: 0 124 171 2141 2700
Size: 124 47 1970 559
Fragments arranged by size:
1970 559 124 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 728 760 2549 2581 2601 2633 2700
Size: 728 32 1789 32 20 32 67
Fragments arranged by size:
1789 728 67 32 32 32 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 766 1593 2478 2628 2700
Size: 766 827 885 150 72
Fragments arranged by size:
885 827 766 150 72
XmnI GAAnn'nnTTC
Cuts at: 0 1424 1713 1930 2700
Size: 1424 289 217 770
Fragments arranged by size:
1424 770 289 217
ZraI GAC'GTC
Cuts at: 0 1358 2700
Size: 1358 1342
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AleI AlwNI ApaI
ApoI AseI AvaI BanI BanII BbsI BcgI BfrBI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BstAPI BstXI BstYI BstZ17I BtgI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FalI FspI Hin4I HincII KpnI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI
PasI PflMI PfoI PpuMI PsiI PspOMI PstI PvuII
SanDI SapI SexAI SfcI SmlI SspI StuI StyI
TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AhdI AlfI AloI
ApaLI AscI AsiSI AvrII BaeI BamHI BbeI BbvCI
BciVI BclI BglI BglII BlpI BmgBI BmtI BsaBI
BsiWI BsrGI BssHII BssSI BstBI BstEII Bsu36I BtgZI
ClaI CspCI EcoICRI EcoRI EcoRV FseI FspAI HaeII
HindIII HpaI KasI MfeI MluI NarI NheI NotI
NruI PacI PciI PmeI PmlI PpiI PshAI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XhoI XmaI