Restriction map for
pF1KB7331
(Linear) (Six-base) MAPSORT of: KIBB7331.seq Check: 9720 from: 1 to: 2982
KIBB7331.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 303 1376 2443 2751 2982
Size: 303 1073 1067 308 231
Fragments arranged by size:
1073 1067 308 303 231
AccI GT'mk_AC
Cuts at: 0 830 2831 2982
Size: 830 2001 151
Fragments arranged by size:
2001 830 151
Acc65I G'GTAC_C
Cuts at: 0 384 1654 2982
Size: 384 1270 1328
Fragments arranged by size:
1328 1270 384
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 1186 1608 2635 2982
Size: 635 551 422 1027 347
Fragments arranged by size:
1027 635 551 422 347
AflIII A'CryG_T
Cuts at: 0 2125 2982
Size: 2125 857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 502 1579 2982
Size: 502 1077 1403
Fragments arranged by size:
1403 1077 502
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 146 178 2982
Size: 146 32 2804
Fragments arranged by size:
2804 146 32
AlwNI CAG_nnn'CTG
Cuts at: 0 539 895 2982
Size: 539 356 2087
Fragments arranged by size:
2087 539 356
ApaI G_GGCC'C
Cuts at: 0 476 1994 2481 2982
Size: 476 1518 487 501
Fragments arranged by size:
1518 501 487 476
ApaLI G'TGCA_C
Cuts at: 0 2185 2982
Size: 2185 797
ApoI r'AATT_y
Cuts at: 0 223 738 2982
Size: 223 515 2244
Fragments arranged by size:
2244 515 223
AvaI C'yCGr_G
Cuts at: 0 1299 1967 2056 2982
Size: 1299 668 89 926
Fragments arranged by size:
1299 926 668 89
AvrII C'CTAG_G
Cuts at: 0 495 2982
Size: 495 2487
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1646 1679 2982
Size: 1646 33 1303
Fragments arranged by size:
1646 1303 33
BamHI G'GATC_C
Cuts at: 0 620 2236 2982
Size: 620 1616 746
Fragments arranged by size:
1616 746 620
BanI G'GyrC_C
Cuts at: 0 384 1030 1171 1654 2109 2191 2539 2648
Size: 384 646 141 483 455 82 348 109
Cuts at: 2648 2739 2982
Size: 91 243
Fragments arranged by size:
646 483 455 384 348 243 141 109
91 82
BanII G_rGCy'C
Cuts at: 0 476 760 1229 1526 1604 1705 1835 1994
Size: 476 284 469 297 78 101 130 159
Cuts at: 1994 2167 2481 2982
Size: 173 314 501
Fragments arranged by size:
501 476 469 314 297 284 173 159
130 101 78
BbeI G_GCGC'C
Cuts at: 0 1175 2982
Size: 1175 1807
BbvCI CC'TCA_GC
Cuts at: 0 262 781 2982
Size: 262 519 2201
Fragments arranged by size:
2201 519 262
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1757 1791 2982
Size: 1757 34 1191
Fragments arranged by size:
1757 1191 34
BglI GCCn_nnn'nGGC
Cuts at: 0 755 2453 2982
Size: 755 1698 529
Fragments arranged by size:
1698 755 529
BlpI GC'TnA_GC
Cuts at: 0 1495 2982
Size: 1495 1487
Bme1580I G_kGCm'C
Cuts at: 0 476 491 1137 1249 1994 2114 2189 2481
Size: 476 15 646 112 745 120 75 292
Cuts at: 2481 2529 2544 2601 2651 2982
Size: 48 15 57 50 331
Fragments arranged by size:
745 646 476 331 292 120 112 75
57 50 48 15 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 393 1734 2982
Size: 393 1341 1248
Fragments arranged by size:
1341 1248 393
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1719 2982
Size: 1719 1263
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 438 452 584 1274 1714 2342 2736
Size: 353 85 14 132 690 440 628 394
Cuts at: 2736 2829 2836 2982
Size: 93 7 146
Fragments arranged by size:
690 628 440 394 353 146 132 93
85 14 7
Bpu10I CC'TnA_GC
Cuts at: 0 143 262 781 1063 2177 2982
Size: 143 119 519 282 1114 805
Fragments arranged by size:
1114 805 519 282 143 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 1360 2488 2982
Size: 238 1122 1128 494
Fragments arranged by size:
1128 1122 494 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 661 2524 2982
Size: 661 1863 458
Fragments arranged by size:
1863 661 458
BsaBI GATnn'nnATC
Cuts at: 0 619 2982
Size: 619 2363
BsaHI Gr'CG_yC
Cuts at: 0 161 1172 2343 2982
Size: 161 1011 1171 639
Fragments arranged by size:
1171 1011 639 161
BsaWI w'CCGG_w
Cuts at: 0 423 2130 2982
Size: 423 1707 852
Fragments arranged by size:
1707 852 423
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 448 769 799 2982
Size: 418 30 321 30 2183
Fragments arranged by size:
2183 418 321 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 271 630 679 2982
Size: 121 150 359 49 2303
Fragments arranged by size:
2303 359 150 121 49
BseYI C'CCAG_C
Cuts at: 0 475 529 1202 2070 2568 2982
Size: 475 54 673 868 498 414
Fragments arranged by size:
868 673 498 475 414 54
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 1354 1797 2982
Size: 281 1073 443 1185
Fragments arranged by size:
1185 1073 443 281
BsiEI CG_ry'CG
Cuts at: 0 1278 1842 2982
Size: 1278 564 1140
Fragments arranged by size:
1278 1140 564
BsiHKAI G_wGCw'C
Cuts at: 0 1526 2189 2336 2982
Size: 1526 663 147 646
Fragments arranged by size:
1526 663 646 147
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1290 2963 2982
Size: 1290 1673 19
Fragments arranged by size:
1673 1290 19
Bsp1286I G_dGCh'C
Cuts at: 0 476 491 760 1137 1229 1249 1526 1604
Size: 476 15 269 377 92 20 277 78
Cuts at: 1604 1705 1835 1994 2114 2167 2189 2336 2481
Size: 101 130 159 120 53 22 147 145
Cuts at: 2481 2529 2544 2601 2651 2982
Size: 48 15 57 50 331
Fragments arranged by size:
476 377 331 277 269 159 147 145
130 120 101 92 78 57 53 50
48 22 20 15 15
BspEI T'CCGG_A
Cuts at: 0 423 2130 2982
Size: 423 1707 852
Fragments arranged by size:
1707 852 423
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 303 1376 1484 2291 2443 2751 2982
Size: 303 1073 108 807 152 308 231
Fragments arranged by size:
1073 807 308 303 231 152 108
BsrDI GCAATG_nn'
Cuts at: 0 1269 1428 1590 2124 2774 2982
Size: 1269 159 162 534 650 208
Fragments arranged by size:
1269 650 534 208 162 159
BsrFI r'CCGG_y
Cuts at: 0 1017 1273 2982
Size: 1017 256 1709
Fragments arranged by size:
1709 1017 256
BssSI C'ACGA_G
Cuts at: 0 2592 2982
Size: 2592 390
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2746 2982
Size: 2746 236
BstBI TT'CG_AA
Cuts at: 0 447 2982
Size: 447 2535
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2320 2760 2982
Size: 2320 440 222
Fragments arranged by size:
2320 440 222
BstYI r'GATC_y
Cuts at: 0 620 2144 2236 2950 2982
Size: 620 1524 92 714 32
Fragments arranged by size:
1524 714 620 92 32
BstZ17I GTA'TAC
Cuts at: 0 2832 2982
Size: 2832 150
Bsu36I CC'TnA_GG
Cuts at: 0 994 1349 2982
Size: 994 355 1633
Fragments arranged by size:
1633 994 355
BtgI C'CryG_G
Cuts at: 0 77 374 580 2021 2195 2369 2514 2982
Size: 77 297 206 1441 174 174 145 468
Fragments arranged by size:
1441 468 297 206 174 174 145 77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2153 2821 2982
Size: 2153 668 161
Fragments arranged by size:
2153 668 161
BtsI GCAGTG_nn'
Cuts at: 0 271 491 922 2982
Size: 271 220 431 2060
Fragments arranged by size:
2060 431 271 220
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 780 815 2982
Size: 780 35 2167
Fragments arranged by size:
2167 780 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 419 2982
Size: 419 2563
EaeI y'GGCC_r
Cuts at: 0 329 932 1275 1825 2982
Size: 329 603 343 550 1157
Fragments arranged by size:
1157 603 550 343 329
EagI C'GGCC_G
Cuts at: 0 1275 2982
Size: 1275 1707
EarI CTCTTCn'nnn_
Cuts at: 0 1871 2155 2982
Size: 1871 284 827
Fragments arranged by size:
1871 827 284
EcoICRI GAG'CTC
Cuts at: 0 1524 2982
Size: 1524 1458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 438 452 584 635 1186 1274 1608
Size: 353 85 14 132 51 551 88 334
Cuts at: 1608 1714 2342 2635 2736 2829 2836 2982
Size: 106 628 293 101 93 7 146
Fragments arranged by size:
628 551 353 334 293 146 132 106
101 93 88 85 51 14 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 232 1393 1696 2982
Size: 232 1161 303 1286
Fragments arranged by size:
1286 1161 303 232
EcoO109I rG'GnC_Cy
Cuts at: 0 69 412 472 559 724 913 1440 1488
Size: 69 343 60 87 165 189 527 48
Cuts at: 1488 1921 1990 1991 2060 2262 2280 2478 2982
Size: 433 69 1 69 202 18 198 504
Fragments arranged by size:
527 504 433 343 202 198 189 165
87 69 69 69 60 48 18 1
EcoRI G'AATT_C
Cuts at: 0 223 2982
Size: 223 2759
FspI TGC'GCA
Cuts at: 0 1915 2982
Size: 1915 1067
HaeII r_GCGC'y
Cuts at: 0 43 1175 2982
Size: 43 1132 1807
Fragments arranged by size:
1807 1132 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 583 615 769 801 2228 2260
Size: 418 32 133 32 154 32 1427 32
Cuts at: 2260 2272 2304 2935 2937 2967 2969 2982
Size: 12 32 631 2 30 2 13
Fragments arranged by size:
1427 631 418 154 133 32 32 32
32 32 30 13 12 2 2
HincII GTy'rAC
Cuts at: 0 1725 2982
Size: 1725 1257
KasI G'GCGC_C
Cuts at: 0 1171 2982
Size: 1171 1811
KpnI G_GTAC'C
Cuts at: 0 388 1658 2982
Size: 388 1270 1324
Fragments arranged by size:
1324 1270 388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2030 2959 2982
Size: 2030 929 23
Fragments arranged by size:
2030 929 23
MscI TGG'CCA
Cuts at: 0 331 934 1827 2982
Size: 331 603 893 1155
Fragments arranged by size:
1155 893 603 331
MslI CAynn'nnrTG
Cuts at: 0 484 1595 1906 2663 2982
Size: 484 1111 311 757 319
Fragments arranged by size:
1111 757 484 319 311
MspA1I CmG'CkG
Cuts at: 0 213 1164 1359 1541 1549 1673 1781 2782
Size: 213 951 195 182 8 124 108 1001
Cuts at: 2782 2874 2982
Size: 92 108
Fragments arranged by size:
1001 951 213 195 182 124 108 108
92 8
NarI GG'CG_CC
Cuts at: 0 1172 2982
Size: 1172 1810
NcoI C'CATG_G
Cuts at: 0 374 2021 2514 2982
Size: 374 1647 493 468
Fragments arranged by size:
1647 493 468 374
NspI r_CATG'y
Cuts at: 0 1873 2129 2982
Size: 1873 256 853
Fragments arranged by size:
1873 853 256
PasI CC'CwG_GG
Cuts at: 0 502 603 1123 1249 2982
Size: 502 101 520 126 1733
Fragments arranged by size:
1733 520 502 126 101
PciI A'CATG_T
Cuts at: 0 2125 2982
Size: 2125 857
PflMI CCAn_nnn'nTGG
Cuts at: 0 1021 1093 2156 2232 2515 2982
Size: 1021 72 1063 76 283 467
Fragments arranged by size:
1063 1021 467 283 76 72
PfoI T'CCnGG_A
Cuts at: 0 561 875 2319 2492 2982
Size: 561 314 1444 173 490
Fragments arranged by size:
1444 561 490 314 173
PpuMI rG'GwC_Cy
Cuts at: 0 412 559 2060 2262 2280 2982
Size: 412 147 1501 202 18 702
Fragments arranged by size:
1501 702 412 202 147 18
PshAI GACnn'nnGTC
Cuts at: 0 2126 2982
Size: 2126 856
PspOMI G'GGCC_C
Cuts at: 0 472 1990 2477 2982
Size: 472 1518 487 505
Fragments arranged by size:
1518 505 487 472
PstI C_TGCA'G
Cuts at: 0 1554 2873 2982
Size: 1554 1319 109
Fragments arranged by size:
1554 1319 109
PvuII CAG'CTG
Cuts at: 0 1164 1549 1673 2782 2874 2982
Size: 1164 385 124 1109 92 108
Fragments arranged by size:
1164 1109 385 124 108 92
SacI G_AGCT'C
Cuts at: 0 1526 2982
Size: 1526 1456
SanDI GG'GwC_CC
Cuts at: 0 412 2982
Size: 412 2570
SapI GCTCTTCn'nnn_
Cuts at: 0 2155 2982
Size: 2155 827
ScaI AGT'ACT
Cuts at: 0 1530 1984 2982
Size: 1530 454 998
Fragments arranged by size:
1530 998 454
SexAI A'CCwGG_T
Cuts at: 0 2225 2982
Size: 2225 757
SfcI C'TryA_G
Cuts at: 0 773 1308 1550 2682 2869 2982
Size: 773 535 242 1132 187 113
Fragments arranged by size:
1132 773 535 242 187 113
SfoI GGC'GCC
Cuts at: 0 1173 2982
Size: 1173 1809
SmaI CCC'GGG
Cuts at: 0 1301 1969 2058 2982
Size: 1301 668 89 924
Fragments arranged by size:
1301 924 668 89
SmlI C'TyrA_G
Cuts at: 0 217 1339 2503 2982
Size: 217 1122 1164 479
Fragments arranged by size:
1164 1122 479 217
StuI AGG'CCT
Cuts at: 0 905 1576 2982
Size: 905 671 1406
Fragments arranged by size:
1406 905 671
StyI C'CwwG_G
Cuts at: 0 374 404 495 2021 2514 2735 2982
Size: 374 30 91 1526 493 221 247
Fragments arranged by size:
1526 493 374 247 221 91 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 994 1136 1216 1653 2582 2691 2982
Size: 994 142 80 437 929 109 291
Fragments arranged by size:
994 929 437 291 142 109 80
TatI w'GTAC_w
Cuts at: 0 1528 1982 2982
Size: 1528 454 1000
Fragments arranged by size:
1528 1000 454
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 263 295 297 329 2982
Size: 263 32 2 32 2653
Fragments arranged by size:
2653 263 32 32 2
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 372 559 1150 1647 2215 2367 2982
Size: 372 187 591 497 568 152 615
Fragments arranged by size:
615 591 568 497 372 187 152
XmaI C'CCGG_G
Cuts at: 0 1299 1967 2056 2982
Size: 1299 668 89 926
Fragments arranged by size:
1299 926 668 89
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI AvrII BaeI BamHI BanI
BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI NspI PasI PciI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
SapI ScaI SexAI SfcI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AleI AscI
AseI AsiSI BbsI BciVI BclI BfrBI BglII BmgBI
BmtI BsaAI BsiWI BsmI BspHI BsrBI BsrGI BssHII
BstEII ClaI DraI DraIII EciI EcoRV FalI FseI
FspAI HindIII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI XmnI ZraI