Restriction map for
pF1KB7347
(Linear) (Six-base) MAPSORT of: KIBB7347.seq Check: 7089 from: 1 to: 3309
KIBB7347.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1093 3309
Size: 1093 2216
AatII G_ACGT'C
Cuts at: 0 2186 3309
Size: 2186 1123
AccI GT'mk_AC
Cuts at: 0 2762 3309
Size: 2762 547
Acc65I G'GTAC_C
Cuts at: 0 1291 3309
Size: 1291 2018
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 1705 1864 2020 3154 3309
Size: 971 734 159 156 1134 155
Fragments arranged by size:
1134 971 734 159 156 155
AfeI AGC'GCT
Cuts at: 0 296 769 3309
Size: 296 473 2540
Fragments arranged by size:
2540 473 296
AflIII A'CryG_T
Cuts at: 0 2852 3309
Size: 2852 457
AleI CACnn'nnGTG
Cuts at: 0 721 3309
Size: 721 2588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2950 3262 3309
Size: 2950 312 47
Fragments arranged by size:
2950 312 47
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1906 1938 3309
Size: 1906 32 1371
Fragments arranged by size:
1906 1371 32
AlwNI CAG_nnn'CTG
Cuts at: 0 576 2568 3309
Size: 576 1992 741
Fragments arranged by size:
1992 741 576
ApaI G_GGCC'C
Cuts at: 0 96 181 621 710 912 3309
Size: 96 85 440 89 202 2397
Fragments arranged by size:
2397 440 202 96 89 85
ApoI r'AATT_y
Cuts at: 0 1690 3309
Size: 1690 1619
AvaI C'yCGr_G
Cuts at: 0 40 89 206 304 620 677 1268 1802
Size: 40 49 117 98 316 57 591 534
Cuts at: 1802 2257 2617 2802 2940 3096 3262 3309
Size: 455 360 185 138 156 166 47
Fragments arranged by size:
591 534 455 360 316 185 166 156
138 117 98 57 49 47 40
AvrII C'CTAG_G
Cuts at: 0 1179 3309
Size: 1179 2130
BamHI G'GATC_C
Cuts at: 0 1260 3309
Size: 1260 2049
BanI G'GyrC_C
Cuts at: 0 441 475 1042 1291 1463 1552 1594 2325
Size: 441 34 567 249 172 89 42 731
Cuts at: 2325 3145 3309
Size: 820 164
Fragments arranged by size:
820 731 567 441 249 172 164 89
42 34
BanII G_rGCy'C
Cuts at: 0 47 69 96 181 213 328 621 659
Size: 47 22 27 85 32 115 293 38
Cuts at: 659 678 710 912 984 1199 1276 1760 1876
Size: 19 32 202 72 215 77 484 116
Cuts at: 1876 2252 2287 2618 2693 3309
Size: 376 35 331 75 616
Fragments arranged by size:
616 484 376 331 293 215 202 116
115 85 77 75 72 47 38 35
32 32 27 22 19
BbeI G_GCGC'C
Cuts at: 0 3149 3309
Size: 3149 160
BbsI GAAGACnn'nnnn_
Cuts at: 0 813 1053 2443 2548 2602 3309
Size: 813 240 1390 105 54 707
Fragments arranged by size:
1390 813 707 240 105 54
BbvCI CC'TCA_GC
Cuts at: 0 2585 3309
Size: 2585 724
BciVI GTATCCnnnnn_n'
Cuts at: 0 2350 3309
Size: 2350 959
BclI T'GATC_A
Cuts at: 0 725 3309
Size: 725 2584
BglI GCCn_nnn'nGGC
Cuts at: 0 235 272 682 1447 3309
Size: 235 37 410 765 1862
Fragments arranged by size:
1862 765 410 235 37
Bme1580I G_kGCm'C
Cuts at: 0 96 181 446 478 621 710 912 1555
Size: 96 85 265 32 143 89 202 643
Cuts at: 1555 1599 2232 2884 3309
Size: 44 633 652 425
Fragments arranged by size:
652 643 633 425 265 202 143 96
89 85 44 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1020 2306 2496 3309
Size: 1020 1286 190 813
Fragments arranged by size:
1286 1020 813 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 799 1402 1735 2176 2266 2491 2512
Size: 692 107 603 333 441 90 225 21
Cuts at: 2512 2545 3301 3309
Size: 33 756 8
Fragments arranged by size:
756 692 603 441 333 225 107 90
33 21 8
Bpu10I CC'TnA_GC
Cuts at: 0 2140 2585 3309
Size: 2140 445 724
Fragments arranged by size:
2140 724 445
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 2023 3084 3309
Size: 968 1055 1061 225
Fragments arranged by size:
1061 1055 968 225
BsaI GGTCTCn'nnnn_
Cuts at: 0 1032 1226 1494 1857 3309
Size: 1032 194 268 363 1452
Fragments arranged by size:
1452 1032 363 268 194
BsaAI yAC'GTr
Cuts at: 0 3057 3309
Size: 3057 252
BsaHI Gr'CG_yC
Cuts at: 0 319 2183 2786 3146 3309
Size: 319 1864 603 360 163
Fragments arranged by size:
1864 603 360 319 163
BsaWI w'CCGG_w
Cuts at: 0 33 378 1120 2091 3309
Size: 33 345 742 971 1218
Fragments arranged by size:
1218 971 742 345 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1115 1145 1661 1691 2605 2635 3309
Size: 1115 30 516 30 914 30 674
Fragments arranged by size:
1115 914 674 516 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 805 1199 1207 1879 2533 3309
Size: 775 30 394 8 672 654 776
Fragments arranged by size:
776 775 672 654 394 30 8
BseYI C'CCAG_C
Cuts at: 0 330 539 750 795 896 1435 1627 1722
Size: 330 209 211 45 101 539 192 95
Cuts at: 1722 2148 2360 2813 3309
Size: 426 212 453 496
Fragments arranged by size:
539 496 453 426 330 212 211 209
192 101 95 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1848 1954 2290 2920 3309
Size: 1848 106 336 630 389
Fragments arranged by size:
1848 630 389 336 106
BsiEI CG_ry'CG
Cuts at: 0 3295 3309
Size: 3295 14
BsiHKAI G_wGCw'C
Cuts at: 0 926 1760 1862 2252 3070 3090 3309
Size: 926 834 102 390 818 20 219
Fragments arranged by size:
926 834 818 390 219 102 20
BsmI GAATG_Cn'
Cuts at: 0 532 3309
Size: 532 2777
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1181 3309
Size: 1181 2128
Bsp1286I G_dGCh'C
Cuts at: 0 47 69 96 181 213 328 446 478
Size: 47 22 27 85 32 115 118 32
Cuts at: 478 621 659 678 710 912 926 984 1199
Size: 143 38 19 32 202 14 58 215
Cuts at: 1199 1276 1555 1599 1760 1862 1876 2232 2252
Size: 77 279 44 161 102 14 356 20
Cuts at: 2252 2287 2618 2693 2884 3070 3090 3309
Size: 35 331 75 191 186 20 219
Fragments arranged by size:
356 331 279 219 215 202 191 186
161 143 118 115 102 85 77 75
58 47 44 38 35 32 32 32
27 22 20 20 19 14 14
BspEI T'CCGG_A
Cuts at: 0 33 1120 3309
Size: 33 1087 2189
Fragments arranged by size:
2189 1087 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1014 1093 1146 2717 3221 3309
Size: 1014 79 53 1571 504 88
Fragments arranged by size:
1571 1014 504 88 79 53
BsrBI CCG'CTC
Cuts at: 0 22 111 2107 3267 3309
Size: 22 89 1996 1160 42
Fragments arranged by size:
1996 1160 89 42 22
BsrFI r'CCGG_y
Cuts at: 0 398 1235 1288 1466 2176 2549 3309
Size: 398 837 53 178 710 373 760
Fragments arranged by size:
837 760 710 398 373 178 53
BssHII G'CGCG_C
Cuts at: 0 544 3309
Size: 544 2765
BssSI C'ACGA_G
Cuts at: 0 370 1341 3309
Size: 370 971 1968
Fragments arranged by size:
1968 971 370
BstAPI GCAn_nnn'nTGC
Cuts at: 0 744 2277 3309
Size: 744 1533 1032
Fragments arranged by size:
1533 1032 744
BstEII G'GTnAC_C
Cuts at: 0 558 3309
Size: 558 2751
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1546 2821 3309
Size: 1546 1275 488
Fragments arranged by size:
1546 1275 488
BstYI r'GATC_y
Cuts at: 0 1260 2496 3254 3309
Size: 1260 1236 758 55
Fragments arranged by size:
1260 1236 758 55
Bsu36I CC'TnA_GG
Cuts at: 0 688 2906 3309
Size: 688 2218 403
Fragments arranged by size:
2218 688 403
BtgI C'CryG_G
Cuts at: 0 479 503 605 650 1517 2052 2058 2315
Size: 479 24 102 45 867 535 6 257
Cuts at: 2315 2390 2876 2980 3234 3309
Size: 75 486 104 254 75
Fragments arranged by size:
867 535 486 479 257 254 104 102
75 75 45 24 6
BtsI GCAGTG_nn'
Cuts at: 0 728 1860 2232 2362 3309
Size: 728 1132 372 130 947
Fragments arranged by size:
1132 947 728 372 130
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1061 1096 3309
Size: 1061 35 2213
Fragments arranged by size:
2213 1061 35
DraIII CAC_nnn'GTG
Cuts at: 0 744 3309
Size: 744 2565
EaeI y'GGCC_r
Cuts at: 0 224 236 400 875 2055 2174 2195 3292
Size: 224 12 164 475 1180 119 21 1097
Cuts at: 3292 3309
Size: 17
Fragments arranged by size:
1180 1097 475 224 164 119 21 17
12
EagI C'GGCC_G
Cuts at: 0 3292 3309
Size: 3292 17
EarI CTCTTCn'nnn_
Cuts at: 0 1883 3309
Size: 1883 1426
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 141 484 1275 1708 3309
Size: 141 343 791 433 1601
Fragments arranged by size:
1601 791 433 343 141
EcoICRI GAG'CTC
Cuts at: 0 1758 2250 3309
Size: 1758 492 1059
Fragments arranged by size:
1758 1059 492
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 799 971 1402 1705 1735 1864 2020
Size: 692 107 172 431 303 30 129 156
Cuts at: 2020 2176 2266 2491 2512 2545 3154 3301 3309
Size: 156 90 225 21 33 609 147 8
Fragments arranged by size:
692 609 431 303 225 172 156 156
147 129 107 90 33 30 21 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 691 778 1831 1951 3309
Size: 691 87 1053 120 1358
Fragments arranged by size:
1358 1053 691 120 87
EcoO109I rG'GnC_Cy
Cuts at: 0 93 171 178 573 617 684 908 1158
Size: 93 78 7 395 44 67 224 250
Cuts at: 1158 1176 1439 1816 1884 2145 3309
Size: 18 263 377 68 261 1164
Fragments arranged by size:
1164 395 377 263 261 250 224 93
78 68 67 44 18 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1755 3309
Size: 1755 1554
FspI TGC'GCA
Cuts at: 0 976 1154 2920 3309
Size: 976 178 1766 389
Fragments arranged by size:
1766 976 389 178
FspAI rTGC'GCAy
Cuts at: 0 2920 3309
Size: 2920 389
HaeII r_GCGC'y
Cuts at: 0 298 771 993 2163 3149 3309
Size: 298 473 222 1170 986 160
Fragments arranged by size:
1170 986 473 298 222 160
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2021 2053 2556 2588 3309
Size: 2021 32 503 32 721
Fragments arranged by size:
2021 721 503 32 32
HincII GTy'rAC
Cuts at: 0 3009 3309
Size: 3009 300
KasI G'GCGC_C
Cuts at: 0 3145 3309
Size: 3145 164
KpnI G_GTAC'C
Cuts at: 0 1295 3309
Size: 1295 2014
MscI TGG'CCA
Cuts at: 0 2197 3309
Size: 2197 1112
MslI CAynn'nnrTG
Cuts at: 0 721 733 1252 1483 1544 2203 3055 3309
Size: 721 12 519 231 61 659 852 254
Fragments arranged by size:
852 721 659 519 254 231 61 12
MspA1I CmG'CkG
Cuts at: 0 505 1129 1622 2118 2817 2982 3309
Size: 505 624 493 496 699 165 327
Fragments arranged by size:
699 624 505 496 493 327 165
NaeI GCC'GGC
Cuts at: 0 400 2178 3309
Size: 400 1778 1131
Fragments arranged by size:
1778 1131 400
NarI GG'CG_CC
Cuts at: 0 3146 3309
Size: 3146 163
NcoI C'CATG_G
Cuts at: 0 650 2315 2390 3309
Size: 650 1665 75 919
Fragments arranged by size:
1665 919 650 75
NdeI CA'TA_TG
Cuts at: 0 2900 3309
Size: 2900 409
NgoMIV G'CCGG_C
Cuts at: 0 398 2176 3309
Size: 398 1778 1133
Fragments arranged by size:
1778 1133 398
NspI r_CATG'y
Cuts at: 0 1257 1482 1608 2856 2895 3309
Size: 1257 225 126 1248 39 414
Fragments arranged by size:
1257 1248 414 225 126 39
PasI CC'CwG_GG
Cuts at: 0 182 446 710 1572 1696 3309
Size: 182 264 264 862 124 1613
Fragments arranged by size:
1613 862 264 264 182 124
PciI A'CATG_T
Cuts at: 0 2852 3309
Size: 2852 457
PflMI CCAn_nnn'nTGG
Cuts at: 0 1039 1638 3309
Size: 1039 599 1671
Fragments arranged by size:
1671 1039 599
PfoI T'CCnGG_A
Cuts at: 0 776 1145 1751 2504 3309
Size: 776 369 606 753 805
Fragments arranged by size:
805 776 753 606 369
PmlI CAC'GTG
Cuts at: 0 3057 3309
Size: 3057 252
PpuMI rG'GwC_Cy
Cuts at: 0 171 573 1158 1176 1884 2145 3309
Size: 171 402 585 18 708 261 1164
Fragments arranged by size:
1164 708 585 402 261 171 18
PspOMI G'GGCC_C
Cuts at: 0 92 177 617 706 908 3309
Size: 92 85 440 89 202 2401
Fragments arranged by size:
2401 440 202 92 89 85
PstI C_TGCA'G
Cuts at: 0 137 1022 3251 3309
Size: 137 885 2229 58
Fragments arranged by size:
2229 885 137 58
PvuII CAG'CTG
Cuts at: 0 1129 2118 3309
Size: 1129 989 1191
Fragments arranged by size:
1191 1129 989
SacI G_AGCT'C
Cuts at: 0 1760 2252 3309
Size: 1760 492 1057
Fragments arranged by size:
1760 1057 492
SacII CC_GC'GG
Cuts at: 0 506 2983 3309
Size: 506 2477 326
Fragments arranged by size:
2477 506 326
SanDI GG'GwC_CC
Cuts at: 0 2145 3309
Size: 2145 1164
SbfI CC_TGCA'GG
Cuts at: 0 3251 3309
Size: 3251 58
ScaI AGT'ACT
Cuts at: 0 2479 2623 3309
Size: 2479 144 686
Fragments arranged by size:
2479 686 144
SexAI A'CCwGG_T
Cuts at: 0 3104 3309
Size: 3104 205
SfcI C'TryA_G
Cuts at: 0 133 1018 3247 3309
Size: 133 885 2229 62
Fragments arranged by size:
2229 885 133 62
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1447 3309
Size: 1447 1862
SfoI GGC'GCC
Cuts at: 0 3147 3309
Size: 3147 162
SmaI CCC'GGG
Cuts at: 0 42 91 622 679 1270 3309
Size: 42 49 531 57 591 2039
Fragments arranged by size:
2039 591 531 57 49 42
SmlI C'TyrA_G
Cuts at: 0 983 2038 3063 3309
Size: 983 1055 1025 246
Fragments arranged by size:
1055 1025 983 246
SphI G_CATG'C
Cuts at: 0 2895 3309
Size: 2895 414
StuI AGG'CCT
Cuts at: 0 871 2590 2933 3309
Size: 871 1719 343 376
Fragments arranged by size:
1719 871 376 343
StyI C'CwwG_G
Cuts at: 0 650 659 872 1179 2306 2315 2390 3309
Size: 650 9 213 307 1127 9 75 919
Fragments arranged by size:
1127 919 650 307 213 75 9 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 570 742 820 1104 1556 1582 3309
Size: 570 172 78 284 452 26 1727
Fragments arranged by size:
1727 570 452 284 172 78 26
TatI w'GTAC_w
Cuts at: 0 2477 2621 2642 3309
Size: 2477 144 21 667
Fragments arranged by size:
2477 667 144 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1115 1147 2104 2136 2849 2881 3069 3101
Size: 1115 32 957 32 713 32 188 32
Cuts at: 3101 3309
Size: 208
Fragments arranged by size:
1115 957 713 208 188 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 1057 3004 3309
Size: 1057 1947 305
Fragments arranged by size:
1947 1057 305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 657 1254 1635 2700 2856 3309
Size: 657 597 381 1065 156 453
Fragments arranged by size:
1065 657 597 453 381 156
XmaI C'CCGG_G
Cuts at: 0 40 89 620 677 1268 3309
Size: 40 49 531 57 591 2041
Fragments arranged by size:
2041 591 531 57 49 40
XmnI GAAnn'nnTTC
Cuts at: 0 1690 3309
Size: 1690 1619
ZraI GAC'GTC
Cuts at: 0 2184 3309
Size: 2184 1125
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AleI
AlfI AloI AlwNI ApaI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI
KpnI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI
PspOMI PstI PvuII SacI SacII SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
ZraI
Enzymes that do not cut:
AclI AflII AgeI AhdI ApaLI AscI AseI AsiSI
BaeI BcgI BfrBI BglII BlpI BmgBI BmrI BmtI
BsaBI BsiWI BspHI BsrDI BsrGI BstBI BstZ17I BtgZI
ClaI DraI DrdI EcoRI EcoRV FseI HindIII HpaI
MfeI MluI MmeI NheI NotI NruI NsiI PacI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SapI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI