Restriction map for
pF1KB7350
(Linear) (Six-base) MAPSORT of: KIBB7350.seq Check: 5490 from: 1 to: 3237
KIBB7350.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 697 910 1297 2065 2683 3237
Size: 697 213 387 768 618 554
Fragments arranged by size:
768 697 618 554 387 213
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1669 1791 2073 2866 3139 3237
Size: 618 1051 122 282 793 273 98
Fragments arranged by size:
1051 793 618 282 273 122 98
AfeI AGC'GCT
Cuts at: 0 365 1183 3237
Size: 365 818 2054
Fragments arranged by size:
2054 818 365
AflIII A'CryG_T
Cuts at: 0 902 3237
Size: 902 2335
AhdI GACnn_n'nnGTC
Cuts at: 0 1695 3237
Size: 1695 1542
AleI CACnn'nnGTG
Cuts at: 0 2785 3237
Size: 2785 452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2273 2487 3237
Size: 2273 214 750
Fragments arranged by size:
2273 750 214
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2395 2427 3237
Size: 2395 32 810
Fragments arranged by size:
2395 810 32
AlwNI CAG_nnn'CTG
Cuts at: 0 333 572 666 1227 1430 1521 2589 3237
Size: 333 239 94 561 203 91 1068 648
Fragments arranged by size:
1068 648 561 333 239 203 94 91
ApaI G_GGCC'C
Cuts at: 0 255 2507 3237
Size: 255 2252 730
Fragments arranged by size:
2252 730 255
ApoI r'AATT_y
Cuts at: 0 525 3237
Size: 525 2712
AvaI C'yCGr_G
Cuts at: 0 649 706 3237
Size: 649 57 2531
Fragments arranged by size:
2531 649 57
BamHI G'GATC_C
Cuts at: 0 710 3237
Size: 710 2527
BanI G'GyrC_C
Cuts at: 0 809 984 1509 2198 2482 3237
Size: 809 175 525 689 284 755
Fragments arranged by size:
809 755 689 525 284 175
BanII G_rGCy'C
Cuts at: 0 255 823 1114 1647 2507 2711 3237
Size: 255 568 291 533 860 204 526
Fragments arranged by size:
860 568 533 526 291 255 204
BbeI G_GCGC'C
Cuts at: 0 2486 3237
Size: 2486 751
BbsI GAAGACnn'nnnn_
Cuts at: 0 624 2139 3033 3237
Size: 624 1515 894 204
Fragments arranged by size:
1515 894 624 204
BbvCI CC'TCA_GC
Cuts at: 0 450 889 1502 3163 3237
Size: 450 439 613 1661 74
Fragments arranged by size:
1661 613 450 439 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1731 1765 3237
Size: 1731 34 1472
Fragments arranged by size:
1731 1472 34
BclI T'GATC_A
Cuts at: 0 2912 3237
Size: 2912 325
BglI GCCn_nnn'nGGC
Cuts at: 0 548 3237
Size: 548 2689
BglII A'GATC_T
Cuts at: 0 2408 3237
Size: 2408 829
BlpI GC'TnA_GC
Cuts at: 0 754 1063 1582 3237
Size: 754 309 519 1655
Fragments arranged by size:
1655 754 519 309
Bme1580I G_kGCm'C
Cuts at: 0 255 989 1786 2507 2680 3226 3237
Size: 255 734 797 721 173 546 11
Fragments arranged by size:
797 734 721 546 255 173 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 104 818 924 2692 3237
Size: 104 714 106 1768 545
Fragments arranged by size:
1768 714 545 106 104
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 779 1170 1320 3006 3237
Size: 779 391 150 1686 231
Fragments arranged by size:
1686 779 391 231 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 408 1128 1136 2483 3016 3237
Size: 326 82 720 8 1347 533 221
Fragments arranged by size:
1347 720 533 326 221 82 8
Bpu10I CC'TnA_GC
Cuts at: 0 450 793 889 1502 3163 3237
Size: 450 343 96 613 1661 74
Fragments arranged by size:
1661 613 450 343 96 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2306 2705 3237
Size: 2306 399 532
Fragments arranged by size:
2306 532 399
BsaI GGTCTCn'nnnn_
Cuts at: 0 96 375 1703 1756 2850 3237
Size: 96 279 1328 53 1094 387
Fragments arranged by size:
1328 1094 387 279 96 53
BsaAI yAC'GTr
Cuts at: 0 1205 1341 1599 3237
Size: 1205 136 258 1638
Fragments arranged by size:
1638 1205 258 136
BsaHI Gr'CG_yC
Cuts at: 0 1301 2483 3038 3237
Size: 1301 1182 555 199
Fragments arranged by size:
1301 1182 555 199
BsaWI w'CCGG_w
Cuts at: 0 2292 3237
Size: 2292 945
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 11 41 422 452 709 739 810 840
Size: 11 30 381 30 257 30 71 30
Cuts at: 840 1377 1407 1858 1888 2395 2425 3237
Size: 537 30 451 30 507 30 812
Fragments arranged by size:
812 537 507 451 381 257 71 30
30 30 30 30 30 30 11
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 298 1398 1519 3198 3237
Size: 295 3 1100 121 1679 39
Fragments arranged by size:
1679 1100 295 121 39 3
BseYI C'CCAG_C
Cuts at: 0 51 728 1078 2019 2880 2904 2961 3121
Size: 51 677 350 941 861 24 57 160
Cuts at: 3121 3237
Size: 116
Fragments arranged by size:
941 861 677 350 160 116 57 51
24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1416 3237
Size: 1416 1821
BsiHKAI G_wGCw'C
Cuts at: 0 1313 1588 2663 3237
Size: 1313 275 1075 574
Fragments arranged by size:
1313 1075 574 275
BsiWI C'GTAC_G
Cuts at: 0 1205 3237
Size: 1205 2032
BsmI GAATG_Cn'
Cuts at: 0 2752 3194 3237
Size: 2752 442 43
Fragments arranged by size:
2752 442 43
BsmBI CGTCTCn'nnnn_
Cuts at: 0 900 3237
Size: 900 2337
Bsp1286I G_dGCh'C
Cuts at: 0 255 823 989 1114 1313 1588 1647 1786
Size: 255 568 166 125 199 275 59 139
Cuts at: 1786 2507 2663 2680 2711 3226 3237
Size: 721 156 17 31 515 11
Fragments arranged by size:
721 568 515 275 255 199 166 156
139 125 59 31 17 11
BspEI T'CCGG_A
Cuts at: 0 2292 3237
Size: 2292 945
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 697 910 1226 1297 2065 2683 3237
Size: 697 213 316 71 768 618 554
Fragments arranged by size:
768 697 618 554 316 213 71
BsrBI CCG'CTC
Cuts at: 0 2305 2517 3237
Size: 2305 212 720
Fragments arranged by size:
2305 720 212
BsrFI r'CCGG_y
Cuts at: 0 1416 3237
Size: 1416 1821
BsrGI T'GTAC_A
Cuts at: 0 381 3237
Size: 381 2856
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1781 3237
Size: 1781 1456
BstEII G'GTnAC_C
Cuts at: 0 56 2077 3237
Size: 56 2021 1160
Fragments arranged by size:
2021 1160 56
BstXI CCAn_nnnn'nTGG
Cuts at: 0 346 619 1611 1843 3138 3237
Size: 346 273 992 232 1295 99
Fragments arranged by size:
1295 992 346 273 232 99
BstYI r'GATC_y
Cuts at: 0 319 710 1517 2408 3158 3237
Size: 319 391 807 891 750 79
Fragments arranged by size:
891 807 750 391 319 79
Bsu36I CC'TnA_GG
Cuts at: 0 805 1295 2734 3237
Size: 805 490 1439 503
Fragments arranged by size:
1439 805 503 490
BtgI C'CryG_G
Cuts at: 0 873 1100 2834 2993 3199 3237
Size: 873 227 1734 159 206 38
Fragments arranged by size:
1734 873 227 206 159 38
BtsI GCAGTG_nn'
Cuts at: 0 19 1894 2228 2255 3237
Size: 19 1875 334 27 982
Fragments arranged by size:
1875 982 334 27 19
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 598 633 2669 2704 3237
Size: 598 35 2036 35 533
Fragments arranged by size:
2036 598 533 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 855 3237
Size: 855 2382
EaeI y'GGCC_r
Cuts at: 0 32 222 876 1335 1601 2213 2459 2558
Size: 32 190 654 459 266 612 246 99
Cuts at: 2558 2801 2837 2969 3237
Size: 243 36 132 268
Fragments arranged by size:
654 612 459 268 266 246 243 190
132 99 36 32
EarI CTCTTCn'nnn_
Cuts at: 0 47 465 479 2170 3237
Size: 47 418 14 1691 1067
Fragments arranged by size:
1691 1067 418 47 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2410 3237
Size: 2410 827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 408 618 1128 1136 1669 1791 2073
Size: 326 82 210 510 8 533 122 282
Cuts at: 2073 2483 2866 3016 3139 3237
Size: 410 383 150 123 98
Fragments arranged by size:
533 510 410 383 326 282 210 150
123 122 98 82 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 277 970 2737 3237
Size: 277 693 1767 500
Fragments arranged by size:
1767 693 500 277
EcoO109I rG'GnC_Cy
Cuts at: 0 136 677 1248 1350 1677 2503 2504 2586
Size: 136 541 571 102 327 826 1 82
Cuts at: 2586 2875 3237
Size: 289 362
Fragments arranged by size:
826 571 541 362 327 289 136 102
82 1
FspI TGC'GCA
Cuts at: 0 3094 3237
Size: 3094 143
HaeII r_GCGC'y
Cuts at: 0 367 1185 2314 2353 2486 2895 3237
Size: 367 818 1129 39 133 409 342
Fragments arranged by size:
1129 818 409 367 342 133 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 420 452 753 785 810 842 1681 1713
Size: 420 32 301 32 25 32 839 32
Cuts at: 1713 2090 2122 3237
Size: 377 32 1115
Fragments arranged by size:
1115 839 420 377 301 32 32 32
32 32 25
HincII GTy'rAC
Cuts at: 0 513 3237
Size: 513 2724
HindIII A'AGCT_T
Cuts at: 0 1130 3086 3237
Size: 1130 1956 151
Fragments arranged by size:
1956 1130 151
KasI G'GCGC_C
Cuts at: 0 2482 3237
Size: 2482 755
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 923 1589 2735 3237
Size: 923 666 1146 502
Fragments arranged by size:
1146 923 666 502
MscI TGG'CCA
Cuts at: 0 34 1337 1603 2215 2560 2803 2839 2971
Size: 34 1303 266 612 345 243 36 132
Cuts at: 2971 3237
Size: 266
Fragments arranged by size:
1303 612 345 266 266 243 132 36
34
MslI CAynn'nnrTG
Cuts at: 0 2785 2812 2839 3237
Size: 2785 27 27 398
Fragments arranged by size:
2785 398 27 27
MspA1I CmG'CkG
Cuts at: 0 732 1026 1062 1102 1430 1530 1839 2607
Size: 732 294 36 40 328 100 309 768
Cuts at: 2607 2628 3167 3237
Size: 21 539 70
Fragments arranged by size:
768 732 539 328 309 294 100 70
40 36 21
NarI GG'CG_CC
Cuts at: 0 2483 3237
Size: 2483 754
NcoI C'CATG_G
Cuts at: 0 2834 3199 3237
Size: 2834 365 38
Fragments arranged by size:
2834 365 38
NspI r_CATG'y
Cuts at: 0 906 1775 3186 3237
Size: 906 869 1411 51
Fragments arranged by size:
1411 906 869 51
PasI CC'CwG_GG
Cuts at: 0 107 814 948 1730 2703 3237
Size: 107 707 134 782 973 534
Fragments arranged by size:
973 782 707 534 134 107
PciI A'CATG_T
Cuts at: 0 902 3237
Size: 902 2335
PfoI T'CCnGG_A
Cuts at: 0 332 424 1271 3237
Size: 332 92 847 1966
Fragments arranged by size:
1966 847 332 92
PmlI CAC'GTG
Cuts at: 0 1341 3237
Size: 1341 1896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2395 2427 3237
Size: 2395 32 810
Fragments arranged by size:
2395 810 32
PpuMI rG'GwC_Cy
Cuts at: 0 136 677 1248 1677 2875 3237
Size: 136 541 571 429 1198 362
Fragments arranged by size:
1198 571 541 429 362 136
PspOMI G'GGCC_C
Cuts at: 0 251 2503 3237
Size: 251 2252 734
Fragments arranged by size:
2252 734 251
PstI C_TGCA'G
Cuts at: 0 122 702 737 842 1223 1481 1832 1901
Size: 122 580 35 105 381 258 351 69
Cuts at: 1901 3237
Size: 1336
Fragments arranged by size:
1336 580 381 351 258 122 105 69
35
PvuII CAG'CTG
Cuts at: 0 732 1026 1062 1430 1530 1839 2607 2628
Size: 732 294 36 368 100 309 768 21
Cuts at: 2628 3167 3237
Size: 539 70
Fragments arranged by size:
768 732 539 368 309 294 100 70
36 21
SacII CC_GC'GG
Cuts at: 0 1103 3237
Size: 1103 2134
SanDI GG'GwC_CC
Cuts at: 0 136 677 1248 2875 3237
Size: 136 541 571 1627 362
Fragments arranged by size:
1627 571 541 362 136
SexAI A'CCwGG_T
Cuts at: 0 1693 3237
Size: 1693 1544
SfcI C'TryA_G
Cuts at: 0 118 698 733 838 1219 1477 1828 1866
Size: 118 580 35 105 381 258 351 38
Cuts at: 1866 1897 3237
Size: 31 1340
Fragments arranged by size:
1340 580 381 351 258 118 105 38
35 31
SfoI GGC'GCC
Cuts at: 0 2484 3237
Size: 2484 753
SmaI CCC'GGG
Cuts at: 0 651 708 3237
Size: 651 57 2529
Fragments arranged by size:
2529 651 57
SmlI C'TyrA_G
Cuts at: 0 2321 2720 3237
Size: 2321 399 517
Fragments arranged by size:
2321 517 399
SphI G_CATG'C
Cuts at: 0 1775 3237
Size: 1775 1462
StuI AGG'CCT
Cuts at: 0 471 3237
Size: 471 2766
StyI C'CwwG_G
Cuts at: 0 233 466 1328 1604 2834 3199 3237
Size: 233 233 862 276 1230 365 38
Fragments arranged by size:
1230 862 365 276 233 233 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 827 2356 2952 3237
Size: 827 1529 596 285
Fragments arranged by size:
1529 827 596 285
TatI w'GTAC_w
Cuts at: 0 381 926 2372 3237
Size: 381 545 1446 865
Fragments arranged by size:
1446 865 545 381
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 11 27 43 59 422 454 1232 1264
Size: 11 16 16 16 363 32 778 32
Cuts at: 1264 1377 1409 3237
Size: 113 32 1828
Fragments arranged by size:
1828 778 363 113 32 32 32 16
16 16 11
Tth111I GACn'n_nGTC
Cuts at: 0 296 1405 1675 3237
Size: 296 1109 270 1562
Fragments arranged by size:
1562 1109 296 270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 404 616 1086 1596 1920 2046 3135 3237
Size: 404 212 470 510 324 126 1089 102
Fragments arranged by size:
1089 510 470 404 324 212 126 102
XmaI C'CCGG_G
Cuts at: 0 649 706 3237
Size: 649 57 2531
Fragments arranged by size:
2531 649 57
XmnI GAAnn'nnTTC
Cuts at: 0 3194 3237
Size: 3194 43
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NarI NcoI NspI PasI PciI PfoI PmlI PpiI
PpuMI PspOMI PstI PvuII SacII SanDI SexAI SfcI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI ApaLI AscI
AseI AsiSI AvrII BaeI BciVI BfrBI BmgBI BmtI
BsaBI BsiEI BspHI BsrDI BssHII BssSI BstBI BstZ17I
BtgZI ClaI DraI DraIII EagI EcoICRI EcoRI EcoRV
FalI FseI FspAI HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI PacI PflMI
PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SapI SbfI ScaI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XbaI XhoI ZraI