Restriction map for
pF1KB7351
(Linear) (Six-base) MAPSORT of: KIBB7351.seq Check: 1764 from: 1 to: 3768
KIBB7351.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 585 3768
Size: 585 3183
AatII G_ACGT'C
Cuts at: 0 3350 3768
Size: 3350 418
AccI GT'mk_AC
Cuts at: 0 1042 2868 3754 3768
Size: 1042 1826 886 14
Fragments arranged by size:
1826 1042 886 14
Acc65I G'GTAC_C
Cuts at: 0 2766 3768
Size: 2766 1002
AclI AA'CG_TT
Cuts at: 0 3675 3768
Size: 3675 93
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 992 1831 1870 2269 2537 2566 2647
Size: 713 279 839 39 399 268 29 81
Cuts at: 2647 2684 3644 3768
Size: 37 960 124
Fragments arranged by size:
960 839 713 399 279 268 124 81
39 37 29
AleI CACnn'nnGTG
Cuts at: 0 2591 3768
Size: 2591 1177
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3206 3768
Size: 3206 562
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1989 2021 3768
Size: 1989 32 1747
Fragments arranged by size:
1989 1747 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1351 3750 3768
Size: 1351 2399 18
Fragments arranged by size:
2399 1351 18
ApoI r'AATT_y
Cuts at: 0 127 438 693 1130 1836 2163 2313 2426
Size: 127 311 255 437 706 327 150 113
Cuts at: 2426 2906 3082 3532 3768
Size: 480 176 450 236
Fragments arranged by size:
706 480 450 437 327 311 255 236
176 150 127 113
AseI AT'TA_AT
Cuts at: 0 3551 3768
Size: 3551 217
AvaI C'yCGr_G
Cuts at: 0 2249 3768
Size: 2249 1519
BamHI G'GATC_C
Cuts at: 0 3096 3768
Size: 3096 672
BanI G'GyrC_C
Cuts at: 0 1404 2766 3190 3768
Size: 1404 1362 424 578
Fragments arranged by size:
1404 1362 578 424
BanII G_rGCy'C
Cuts at: 0 87 617 2239 3641 3768
Size: 87 530 1622 1402 127
Fragments arranged by size:
1622 1402 530 127 87
BbsI GAAGACnn'nnnn_
Cuts at: 0 167 347 1032 2991 3528 3768
Size: 167 180 685 1959 537 240
Fragments arranged by size:
1959 685 537 240 180 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1900 1934 3768
Size: 1900 34 1834
Fragments arranged by size:
1900 1834 34
BclI T'GATC_A
Cuts at: 0 1824 3768
Size: 1824 1944
BfrBI ATG'CAT
Cuts at: 0 1657 3768
Size: 1657 2111
BglI GCCn_nnn'nGGC
Cuts at: 0 154 3768
Size: 154 3614
BlpI GC'TnA_GC
Cuts at: 0 1295 3768
Size: 1295 2473
Bme1580I G_kGCm'C
Cuts at: 0 2424 2754 3768
Size: 2424 330 1014
Fragments arranged by size:
2424 1014 330
BmrI ACTGGGnnnn_n'
Cuts at: 0 1688 3768
Size: 1688 2080
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1142 1373 3768
Size: 1142 231 2395
Fragments arranged by size:
2395 1142 231
Bpu10I CC'TnA_GC
Cuts at: 0 260 512 2214 3768
Size: 260 252 1702 1554
Fragments arranged by size:
1702 1554 260 252
BsaI GGTCTCn'nnnn_
Cuts at: 0 3457 3768
Size: 3457 311
BsaBI GATnn'nnATC
Cuts at: 0 247 3768
Size: 247 3521
BsaHI Gr'CG_yC
Cuts at: 0 2865 3347 3768
Size: 2865 482 421
Fragments arranged by size:
2865 482 421
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 784 2024 2488 2491 2815 3768
Size: 784 1240 464 3 324 953
Fragments arranged by size:
1240 953 784 464 324 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2813 3362 3743 3768
Size: 2813 549 381 25
Fragments arranged by size:
2813 549 381 25
BsiHKAI G_wGCw'C
Cuts at: 0 62 1158 1359 1755 2034 2239 3768
Size: 62 1096 201 396 279 205 1529
Fragments arranged by size:
1529 1096 396 279 205 201 62
BsmI GAATG_Cn'
Cuts at: 0 893 3248 3521 3768
Size: 893 2355 273 247
Fragments arranged by size:
2355 893 273 247
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1308 3768
Size: 1308 2460
Bsp1286I G_dGCh'C
Cuts at: 0 62 87 617 1158 1359 1755 2034 2239
Size: 62 25 530 541 201 396 279 205
Cuts at: 2239 2424 2754 3641 3768
Size: 185 330 887 127
Fragments arranged by size:
887 541 530 396 330 279 205 201
185 127 62 25
BspHI T'CATG_A
Cuts at: 0 2730 3030 3593 3768
Size: 2730 300 563 175
Fragments arranged by size:
2730 563 300 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 585 2582 2778 3768
Size: 585 1997 196 990
Fragments arranged by size:
1997 990 585 196
BsrBI CCG'CTC
Cuts at: 0 12 89 3768
Size: 12 77 3679
Fragments arranged by size:
3679 77 12
BssSI C'ACGA_G
Cuts at: 0 3068 3768
Size: 3068 700
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3141 3243 3768
Size: 3141 102 525
Fragments arranged by size:
3141 525 102
BstYI r'GATC_y
Cuts at: 0 462 966 3096 3768
Size: 462 504 2130 672
Fragments arranged by size:
2130 672 504 462
BstZ17I GTA'TAC
Cuts at: 0 1043 3768
Size: 1043 2725
Bsu36I CC'TnA_GG
Cuts at: 0 303 2996 3768
Size: 303 2693 772
Fragments arranged by size:
2693 772 303
BtgI C'CryG_G
Cuts at: 0 2936 3768
Size: 2936 832
BtsI GCAGTG_nn'
Cuts at: 0 1551 3768
Size: 1551 2217
DraIII CAC_nnn'GTG
Cuts at: 0 66 3768
Size: 66 3702
EaeI y'GGCC_r
Cuts at: 0 20 155 3768
Size: 20 135 3613
Fragments arranged by size:
3613 135 20
EarI CTCTTCn'nnn_
Cuts at: 0 39 131 491 1415 2438 2492 2666 2792
Size: 39 92 360 924 1023 54 174 126
Cuts at: 2792 2852 3154 3768
Size: 60 302 614
Fragments arranged by size:
1023 924 614 360 302 174 126 92
60 54 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3637 3768
Size: 3637 131
EcoICRI GAG'CTC
Cuts at: 0 2237 3768
Size: 2237 1531
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 992 1142 1373 1831 1870 2269 2537
Size: 713 279 150 231 458 39 399 268
Cuts at: 2537 2566 2647 2684 3644 3768
Size: 29 81 37 960 124
Fragments arranged by size:
960 713 458 399 279 268 231 150
124 81 39 37 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1208 3768
Size: 1208 2560
EcoO109I rG'GnC_Cy
Cuts at: 0 703 3747 3768
Size: 703 3044 21
Fragments arranged by size:
3044 703 21
EcoRI G'AATT_C
Cuts at: 0 2426 3768
Size: 2426 1342
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2714 3768
Size: 2714 1054
FspI TGC'GCA
Cuts at: 0 1543 3768
Size: 1543 2225
HaeII r_GCGC'y
Cuts at: 0 1562 3768
Size: 1562 2206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 1578 1610 3439 3471 3601 3633
Size: 490 32 1056 32 1829 32 130 32
Cuts at: 3633 3768
Size: 135
Fragments arranged by size:
1829 1056 490 135 130 32 32 32
32
HincII GTy'rAC
Cuts at: 0 286 2706 3201 3579 3755 3768
Size: 286 2420 495 378 176 13
Fragments arranged by size:
2420 495 378 286 176 13
HindIII A'AGCT_T
Cuts at: 0 223 3768
Size: 223 3545
KpnI G_GTAC'C
Cuts at: 0 2770 3768
Size: 2770 998
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 881 1262 1776 2299 3768
Size: 155 726 381 514 523 1469
Fragments arranged by size:
1469 726 523 514 381 155
MslI CAynn'nnrTG
Cuts at: 0 199 2591 3768
Size: 199 2392 1177
Fragments arranged by size:
2392 1177 199
MspA1I CmG'CkG
Cuts at: 0 25 3693 3768
Size: 25 3668 75
Fragments arranged by size:
3668 75 25
NcoI C'CATG_G
Cuts at: 0 2936 3768
Size: 2936 832
NdeI CA'TA_TG
Cuts at: 0 738 2127 2222 3768
Size: 738 1389 95 1546
Fragments arranged by size:
1546 1389 738 95
NsiI A_TGCA'T
Cuts at: 0 1659 3768
Size: 1659 2109
PasI CC'CwG_GG
Cuts at: 0 2009 3768
Size: 2009 1759
PfoI T'CCnGG_A
Cuts at: 0 1675 3717 3768
Size: 1675 2042 51
Fragments arranged by size:
2042 1675 51
PpuMI rG'GwC_Cy
Cuts at: 0 703 3768
Size: 703 3065
PstI C_TGCA'G
Cuts at: 0 1740 3768
Size: 1740 2028
PvuII CAG'CTG
Cuts at: 0 3693 3768
Size: 3693 75
RsrII CG'GwC_CG
Cuts at: 0 39 3768
Size: 39 3729
SacI G_AGCT'C
Cuts at: 0 2239 3768
Size: 2239 1529
SalI G'TCGA_C
Cuts at: 0 3753 3768
Size: 3753 15
SapI GCTCTTCn'nnn_
Cuts at: 0 131 2492 2666 3768
Size: 131 2361 174 1102
Fragments arranged by size:
2361 1102 174 131
ScaI AGT'ACT
Cuts at: 0 609 3768
Size: 609 3159
SfcI C'TryA_G
Cuts at: 0 1197 1736 2648 3626 3768
Size: 1197 539 912 978 142
Fragments arranged by size:
1197 978 912 539 142
SpeI A'CTAG_T
Cuts at: 0 3259 3768
Size: 3259 509
StyI C'CwwG_G
Cuts at: 0 1563 2936 3768
Size: 1563 1373 832
Fragments arranged by size:
1563 1373 832
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 27 1512 1777 3768
Size: 27 1485 265 1991
Fragments arranged by size:
1991 1485 265 27
TatI w'GTAC_w
Cuts at: 0 607 3768
Size: 607 3161
XbaI T'CTAG_A
Cuts at: 0 54 2154 3768
Size: 54 2100 1614
Fragments arranged by size:
2100 1614 54
XmnI GAAnn'nnTTC
Cuts at: 0 746 1029 1244 1684 3676 3768
Size: 746 283 215 440 1992 92
Fragments arranged by size:
1992 746 440 283 215 92
ZraI GAC'GTC
Cuts at: 0 3348 3768
Size: 3348 420
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AleI AlfI
AloI AlwNI ApoI AseI AvaI BamHI BanI BanII
BbsI BcgI BclI BfrBI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaBI BsaHI BseRI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BssSI BstAPI
BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KpnI MmeI MslI MspA1I
NcoI NdeI NsiI PasI PfoI PpuMI PstI PvuII
RsrII SacI SalI SapI ScaI SfcI SpeI StyI
TaqII TatI XbaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AflIII AgeI AhdI ApaI ApaLI AscI
AsiSI AvrII BaeI BbeI BbvCI BciVI BglII BmgBI
BmtI BplI BpuEI BsaAI BsaWI BsaXI BseYI BsiEI
BsiWI BspEI BsrDI BsrFI BsrGI BssHII BstBI BstEII
BstXI BtgZI ClaI CspCI DraI DrdI EagI EcoRV
FseI FspAI HpaI KasI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI SacII SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SphI SrfI SspI
StuI SwaI TstI Tth111I XcmI XhoI XmaI