Restriction map for
pF1KB7355
(Linear) (Six-base) MAPSORT of: KIBB7355.seq Check: 4687 from: 1 to: 3264
KIBB7355.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AatII G_ACGT'C
Cuts at: 0 1473 3264
Size: 1473 1791
AccI GT'mk_AC
Cuts at: 0 1487 3264
Size: 1487 1777
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 491 601 1076 1822 1965 2239 2373
Size: 369 122 110 475 746 143 274 134
Cuts at: 2373 3099 3264
Size: 726 165
Fragments arranged by size:
746 726 475 369 274 165 143 134
122 110
AfeI AGC'GCT
Cuts at: 0 2009 3264
Size: 2009 1255
AflIII A'CryG_T
Cuts at: 0 193 1366 2290 3264
Size: 193 1173 924 974
Fragments arranged by size:
1173 974 924 193
AleI CACnn'nnGTG
Cuts at: 0 1928 3264
Size: 1928 1336
AlwNI CAG_nnn'CTG
Cuts at: 0 2433 2798 3147 3264
Size: 2433 365 349 117
Fragments arranged by size:
2433 365 349 117
ApaLI G'TGCA_C
Cuts at: 0 1335 3264
Size: 1335 1929
ApoI r'AATT_y
Cuts at: 0 982 1108 1906 2053 2061 2352 2378 3264
Size: 982 126 798 147 8 291 26 886
Fragments arranged by size:
982 886 798 291 147 126 26 8
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2768 2801 3264
Size: 2768 33 463
Fragments arranged by size:
2768 463 33
BanII G_rGCy'C
Cuts at: 0 3023 3264
Size: 3023 241
BbsI GAAGACnn'nnnn_
Cuts at: 0 375 2541 2984 3264
Size: 375 2166 443 280
Fragments arranged by size:
2166 443 375 280
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1381 1415 3264
Size: 1381 34 1849
Fragments arranged by size:
1849 1381 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 929 1593 3012 3264
Size: 929 664 1419 252
Fragments arranged by size:
1419 929 664 252
BclI T'GATC_A
Cuts at: 0 1686 3264
Size: 1686 1578
BglII A'GATC_T
Cuts at: 0 2031 2166 3264
Size: 2031 135 1098
Fragments arranged by size:
2031 1098 135
BlpI GC'TnA_GC
Cuts at: 0 176 3264
Size: 176 3088
Bme1580I G_kGCm'C
Cuts at: 0 1339 3264
Size: 1339 1925
BmrI ACTGGGnnnn_n'
Cuts at: 0 1113 1812 3102 3264
Size: 1113 699 1290 162
Fragments arranged by size:
1290 1113 699 162
BmtI G_CTAG'C
Cuts at: 0 574 3264
Size: 574 2690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 2572 2775 3264
Size: 403 2169 203 489
Fragments arranged by size:
2169 489 403 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2464 2494 3264
Size: 2464 30 770
Fragments arranged by size:
2464 770 30
BsaAI yAC'GTr
Cuts at: 0 276 3264
Size: 276 2988
BsaBI GATnn'nnATC
Cuts at: 0 250 3264
Size: 250 3014
BsaHI Gr'CG_yC
Cuts at: 0 1470 2546 3264
Size: 1470 1076 718
Fragments arranged by size:
1470 1076 718
BsaWI w'CCGG_w
Cuts at: 0 1603 2642 3264
Size: 1603 1039 622
Fragments arranged by size:
1603 1039 622
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1019 1049 1318 1348 3264
Size: 1019 30 269 30 1916
Fragments arranged by size:
1916 1019 269 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2778 3264
Size: 2778 486
BseYI C'CCAG_C
Cuts at: 0 1023 2342 3264
Size: 1023 1319 922
Fragments arranged by size:
1319 1023 922
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 1402 3264
Size: 754 648 1862
Fragments arranged by size:
1862 754 648
BsiHKAI G_wGCw'C
Cuts at: 0 1339 1374 3264
Size: 1339 35 1890
Fragments arranged by size:
1890 1339 35
BsmI GAATG_Cn'
Cuts at: 0 2265 3264
Size: 2265 999
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1759 3264
Size: 1759 1505
Bsp1286I G_dGCh'C
Cuts at: 0 1339 1374 3023 3264
Size: 1339 35 1649 241
Fragments arranged by size:
1649 1339 241 35
BspHI T'CATG_A
Cuts at: 0 978 1772 2957 3264
Size: 978 794 1185 307
Fragments arranged by size:
1185 978 794 307
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 639 3264
Size: 639 2625
BsrDI GCAATG_nn'
Cuts at: 0 329 3264
Size: 329 2935
BsrGI T'GTAC_A
Cuts at: 0 2293 3264
Size: 2293 971
BssSI C'ACGA_G
Cuts at: 0 1926 3264
Size: 1926 1338
BstXI CCAn_nnnn'nTGG
Cuts at: 0 749 3264
Size: 749 2515
BstYI r'GATC_y
Cuts at: 0 783 2031 2166 2338 2429 3264
Size: 783 1248 135 172 91 835
Fragments arranged by size:
1248 835 783 172 135 91
BstZ17I GTA'TAC
Cuts at: 0 1488 3264
Size: 1488 1776
Bsu36I CC'TnA_GG
Cuts at: 0 950 3264
Size: 950 2314
BtsI GCAGTG_nn'
Cuts at: 0 435 3228 3264
Size: 435 2793 36
Fragments arranged by size:
2793 435 36
DraI TTT'AAA
Cuts at: 0 587 611 1007 1877 2124 3264
Size: 587 24 396 870 247 1140
Fragments arranged by size:
1140 870 587 396 247 24
DrdI GACnn_nn'nnGTC
Cuts at: 0 379 870 2818 3264
Size: 379 491 1948 446
Fragments arranged by size:
1948 491 446 379
EarI CTCTTCn'nnn_
Cuts at: 0 1379 1841 2392 2836 3192 3264
Size: 1379 462 551 444 356 72
Fragments arranged by size:
1379 551 462 444 356 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 403 491 601 1076 1822 1965 2239
Size: 369 34 88 110 475 746 143 274
Cuts at: 2239 2373 2572 2775 3099 3264
Size: 134 199 203 324 165
Fragments arranged by size:
746 475 369 324 274 203 199 165
143 134 110 88 34
EcoO109I rG'GnC_Cy
Cuts at: 0 872 3099 3264
Size: 872 2227 165
Fragments arranged by size:
2227 872 165
EcoRI G'AATT_C
Cuts at: 0 2378 3264
Size: 2378 886
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1489 2535 3264
Size: 1489 1046 729
Fragments arranged by size:
1489 1046 729
FspI TGC'GCA
Cuts at: 0 3220 3264
Size: 3220 44
HaeII r_GCGC'y
Cuts at: 0 7 2011 3264
Size: 7 2004 1253
Fragments arranged by size:
2004 1253 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 440 472 1002 1034 1356 1388 2016 2048
Size: 440 32 530 32 322 32 628 32
Cuts at: 2048 2350 2382 3264
Size: 302 32 882
Fragments arranged by size:
882 628 530 440 322 302 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 142 3264
Size: 142 3122
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1229 1304 2965 3264
Size: 766 463 75 1661 299
Fragments arranged by size:
1661 766 463 299 75
MslI CAynn'nnrTG
Cuts at: 0 840 1267 1283 1928 2868 3264
Size: 840 427 16 645 940 396
Fragments arranged by size:
940 840 645 427 396 16
MspA1I CmG'CkG
Cuts at: 0 1023 2795 3264
Size: 1023 1772 469
Fragments arranged by size:
1772 1023 469
NdeI CA'TA_TG
Cuts at: 0 1677 2696 3264
Size: 1677 1019 568
Fragments arranged by size:
1677 1019 568
NheI G'CTAG_C
Cuts at: 0 570 3264
Size: 570 2694
NspI r_CATG'y
Cuts at: 0 197 578 1370 2294 3264
Size: 197 381 792 924 970
Fragments arranged by size:
970 924 792 381 197
PacI TTA_AT'TAA
Cuts at: 0 920 3264
Size: 920 2344
PciI A'CATG_T
Cuts at: 0 193 1366 2290 3264
Size: 193 1173 924 974
Fragments arranged by size:
1173 974 924 193
PflMI CCAn_nnn'nTGG
Cuts at: 0 21 1435 3264
Size: 21 1414 1829
Fragments arranged by size:
1829 1414 21
PfoI T'CCnGG_A
Cuts at: 0 764 2672 3264
Size: 764 1908 592
Fragments arranged by size:
1908 764 592
PpuMI rG'GwC_Cy
Cuts at: 0 872 3099 3264
Size: 872 2227 165
Fragments arranged by size:
2227 872 165
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1151 1183 3264
Size: 1151 32 2081
Fragments arranged by size:
2081 1151 32
PstI C_TGCA'G
Cuts at: 0 717 2939 3264
Size: 717 2222 325
Fragments arranged by size:
2222 717 325
PvuII CAG'CTG
Cuts at: 0 1023 2795 3264
Size: 1023 1772 469
Fragments arranged by size:
1772 1023 469
SapI GCTCTTCn'nnn_
Cuts at: 0 1379 3264
Size: 1379 1885
SexAI A'CCwGG_T
Cuts at: 0 485 3264
Size: 485 2779
SfcI C'TryA_G
Cuts at: 0 713 1096 2935 3264
Size: 713 383 1839 329
Fragments arranged by size:
1839 713 383 329
SmlI C'TyrA_G
Cuts at: 0 2479 2509 3264
Size: 2479 30 755
Fragments arranged by size:
2479 755 30
SphI G_CATG'C
Cuts at: 0 578 3264
Size: 578 2686
SspI AAT'ATT
Cuts at: 0 2108 3264
Size: 2108 1156
StuI AGG'CCT
Cuts at: 0 1231 3009 3264
Size: 1231 1778 255
Fragments arranged by size:
1778 1231 255
StyI C'CwwG_G
Cuts at: 0 906 2439 3264
Size: 906 1533 825
Fragments arranged by size:
1533 906 825
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2026 3190 3264
Size: 2026 1164 74
Fragments arranged by size:
2026 1164 74
TatI w'GTAC_w
Cuts at: 0 43 1531 2293 2583 2727 3264
Size: 43 1488 762 290 144 537
Fragments arranged by size:
1488 762 537 290 144 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1318 1350 3264
Size: 1318 32 1914
Fragments arranged by size:
1914 1318 32
XbaI T'CTAG_A
Cuts at: 0 499 1761 3264
Size: 499 1262 1503
Fragments arranged by size:
1503 1262 499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3012 3264
Size: 3012 252
XmnI GAAnn'nnTTC
Cuts at: 0 3043 3264
Size: 3043 221
ZraI GAC'GTC
Cuts at: 0 1471 3264
Size: 1471 1793
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AleI AlwNI ApaLI
ApoI BaeI BanII BbsI BcgI BciVI BclI BglII
BlpI Bme1580I BmrI BmtI BpmI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstXI
BstYI BstZ17I Bsu36I BtsI DraI DrdI EarI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII MmeI
MslI MspA1I NdeI NheI NspI PacI PciI PflMI
PfoI PpuMI PsrI PstI PvuII SapI SexAI SfcI
SmlI SphI SspI StuI StyI TaqII TatI TstI
XbaI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AlfI AloI
ApaI AscI AseI AsiSI AvaI AvrII BamHI BanI
BbeI BbvCI BfrBI BglI BmgBI BplI Bpu10I BsaI
BsiEI BsiWI BspEI BsrBI BsrFI BssHII BstAPI BstBI
BstEII BtgI BtgZI ClaI CspCI DraIII EaeI EagI
EciI EcoICRI EcoNI EcoRV FseI FspAI HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NgoMIV NotI NruI NsiI PasI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI