(Linear) (Six-base) MAPSORT of: KIBB7359.seq  Check: 6342  from: 1  to: 1017



KIBB7359.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AflIII A'CryG_T

Cuts at:      0     308    1017 
   Size:        308     709
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     211     419     776    1017 
   Size:        211     208     357     241

  Fragments arranged by size: 

                 357     241     211     208
 
ApaLI G'TGCA_C

Cuts at:      0      60    1017 
   Size:         60     957
 
ApoI r'AATT_y

Cuts at:      0     412     725     999    1017 
   Size:        412     313     274      18

  Fragments arranged by size: 

                 412     313     274      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     615     624    1017 
   Size:        615       9     393

  Fragments arranged by size: 

                 615     393       9
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     375     409    1017 
   Size:        375      34     608

  Fragments arranged by size: 

                 608     375      34
 
Bme1580I G_kGCm'C

Cuts at:      0      64    1017 
   Size:         64     953
 
BmgBI CAC'GTC

Cuts at:      0      48    1017 
   Size:         48     969
 
Bpu10I CC'TnA_GC

Cuts at:      0     407    1017 
   Size:        407     610
 
BsaWI w'CCGG_w

Cuts at:      0     914    1017 
   Size:        914     103
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     451     481    1017 
   Size:        451      30     536

  Fragments arranged by size: 

                 536     451      30
 
BseYI C'CCAG_C

Cuts at:      0     315     375     548    1017 
   Size:        315      60     173     469

  Fragments arranged by size: 

                 469     315     173      60
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     962    1017 
   Size:        962      55
 
BsiHKAI G_wGCw'C

Cuts at:      0      64    1017 
   Size:         64     953
 
BsmI GAATG_Cn'

Cuts at:      0     171     667    1017 
   Size:        171     496     350

  Fragments arranged by size: 

                 496     350     171
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      20    1017 
   Size:         20     997
 
Bsp1286I G_dGCh'C

Cuts at:      0      64    1017 
   Size:         64     953
 
BspEI T'CCGG_A

Cuts at:      0     914    1017 
   Size:        914     103
 
BspHI T'CATG_A

Cuts at:      0     441    1017 
   Size:        441     576
 
BsrBI CCG'CTC

Cuts at:      0     238    1017 
   Size:        238     779
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     155    1017 
   Size:        155     862
 
BstYI r'GATC_y

Cuts at:      0     493    1017 
   Size:        493     524
 
BtsI GCAGTG_nn'

Cuts at:      0     276     281     729     950     983    1017 
   Size:        276       5     448     221      33      34

  Fragments arranged by size: 

                 448     276     221      34      33       5
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     270     305    1017 
   Size:        270      35     712

  Fragments arranged by size: 

                 712     270      35
 
DraI TTT'AAA

Cuts at:      0     590    1017 
   Size:        590     427
 
EaeI y'GGCC_r

Cuts at:      0     975    1017 
   Size:        975      42
 
EarI CTCTTCn'nnn_

Cuts at:      0     554    1017 
   Size:        554     463
 
EcoRI G'AATT_C

Cuts at:      0     412     999    1017 
   Size:        412     587      18

  Fragments arranged by size: 

                 587     412      18
 
EcoRV GAT'ATC

Cuts at:      0      39    1017 
   Size:         39     978
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     319     351     478     510    1017 
   Size:        319      32     127      32     507

  Fragments arranged by size: 

                 507     319     127      32      32
 
MscI TGG'CCA

Cuts at:      0     977    1017 
   Size:        977      40
 
MslI CAynn'nnrTG

Cuts at:      0     959    1017 
   Size:        959      58
 
MspA1I CmG'CkG

Cuts at:      0     201     552    1017 
   Size:        201     351     465

  Fragments arranged by size: 

                 465     351     201
 
NdeI CA'TA_TG

Cuts at:      0     483    1017 
   Size:        483     534
 
NspI r_CATG'y

Cuts at:      0     158     312    1017 
   Size:        158     154     705

  Fragments arranged by size: 

                 705     158     154
 
PciI A'CATG_T

Cuts at:      0     308    1017 
   Size:        308     709
 
PfoI T'CCnGG_A

Cuts at:      0     469    1017 
   Size:        469     548
 
PstI C_TGCA'G

Cuts at:      0      27     190     514    1017 
   Size:         27     163     324     503

  Fragments arranged by size: 

                 503     324     163      27
 
PvuII CAG'CTG

Cuts at:      0     552    1017 
   Size:        552     465
 
SapI GCTCTTCn'nnn_

Cuts at:      0     554    1017 
   Size:        554     463
 
SfcI C'TryA_G

Cuts at:      0      23     186     510    1017 
   Size:         23     163     324     507

  Fragments arranged by size: 

                 507     324     163      23
 
SspI AAT'ATT

Cuts at:      0     347    1017 
   Size:        347     670
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     562    1017 
   Size:        562     455
 
Tth111I GACn'n_nGTC

Cuts at:      0     331    1017 
   Size:        331     686
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     347    1017 
   Size:        347     670
 
XmnI GAAnn'nnTTC

Cuts at:      0     212    1017 
   Size:        212     805
 

 Enzymes that do cut:

   AflIII     AlfI    ApaLI     ApoI     BbsI     BcgI Bme1580I    BmgBI
   Bpu10I    BsaWI    BsaXI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BsrBI   BstAPI    BstYI     BtsI    CspCI
     DraI     EaeI     EarI    EcoRI    EcoRV    Hin4I     MscI     MslI
   MspA1I     NdeI     NspI     PciI     PfoI     PstI    PvuII     SapI
     SfcI     SspI    TaqII  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BseRI    BsiEI    BsiWI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
     TatI     TstI     XbaI     XhoI     XmaI     ZraI