Restriction map for pF1KB7361
 (Linear) (Six-base) MAPSORT of: KIBB7361.seq  Check: 8110  from: 1  to: 927



KIBB7361.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     764     790     927 
   Size:        296     468      26     137

  Fragments arranged by size: 

                 468     296     137      26
 
AleI CACnn'nnGTG

Cuts at:      0      16     166     927 
   Size:         16     150     761

  Fragments arranged by size: 

                 761     150      16
 
AlwNI CAG_nnn'CTG

Cuts at:      0     319     927 
   Size:        319     608
 
ApoI r'AATT_y

Cuts at:      0      25     567     927 
   Size:         25     542     360

  Fragments arranged by size: 

                 542     360      25
 
AseI AT'TA_AT

Cuts at:      0     599     927 
   Size:        599     328
 
BanI G'GyrC_C

Cuts at:      0     478     927 
   Size:        478     449
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     648     927 
   Size:        648     279
 
BclI T'GATC_A

Cuts at:      0     134     927 
   Size:        134     793
 
Bme1580I G_kGCm'C

Cuts at:      0     108     927 
   Size:        108     819
 
BmgBI CAC'GTC

Cuts at:      0     654     927 
   Size:        654     273
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     534     927 
   Size:        534     393
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     247     277     399     429     927 
   Size:        247      30     122      30     498

  Fragments arranged by size: 

                 498     247     122      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     322     325     927 
   Size:        322       3     602

  Fragments arranged by size: 

                 602     322       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     367     927 
   Size:        367     560
 
BsiHKAI G_wGCw'C

Cuts at:      0     412     927 
   Size:        412     515
 
BsmI GAATG_Cn'

Cuts at:      0      72     927 
   Size:         72     855
 
Bsp1286I G_dGCh'C

Cuts at:      0     108     412     927 
   Size:        108     304     515

  Fragments arranged by size: 

                 515     304     108
 
BspHI T'CATG_A

Cuts at:      0     131     927 
   Size:        131     796
 
BsrDI GCAATG_nn'

Cuts at:      0     758     927 
   Size:        758     169
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     885     927 
   Size:        885      42
 
BstYI r'GATC_y

Cuts at:      0     201     674     927 
   Size:        201     473     253

  Fragments arranged by size: 

                 473     253     201
 
EarI CTCTTCn'nnn_

Cuts at:      0      88     927 
   Size:         88     839
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     764     790     927 
   Size:        296     468      26     137

  Fragments arranged by size: 

                 468     296     137      26
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     530     927 
   Size:        530     397
 
MslI CAynn'nnrTG

Cuts at:      0      16     130     166     343     389     883     927 
   Size:         16     114      36     177      46     494      44

  Fragments arranged by size: 

                 494     177     114      46      44      36      16
 
MspA1I CmG'CkG

Cuts at:      0     475     927 
   Size:        475     452
 
PstI C_TGCA'G

Cuts at:      0     324     927 
   Size:        324     603
 
PvuII CAG'CTG

Cuts at:      0     475     927 
   Size:        475     452
 
SfcI C'TryA_G

Cuts at:      0     320     662     927 
   Size:        320     342     265

  Fragments arranged by size: 

                 342     320     265
 
StyI C'CwwG_G

Cuts at:      0     276     927 
   Size:        276     651
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     247     279     399     431     927 
   Size:        247      32     120      32     496

  Fragments arranged by size: 

                 496     247     120      32      32
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI     AseI     BanI     BbsI     BclI
 Bme1580I    BmgBI     BmrI    BsaXI    BseRI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BspHI    BsrDI    BstXI    BstYI     EarI  Eco57MI     MmeI
     MslI   MspA1I     PstI    PvuII     SfcI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI
    BsiEI    BsiWI    BsmBI    BspEI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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