(Linear) (Six-base) MAPSORT of: KIBB7361.seq Check: 8110 from: 1 to: 927 KIBB7361.orf With 182 enzymes: SgfI * March 3, 2008 11:00 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 764 790 927 Size: 296 468 26 137 Fragments arranged by size: 468 296 137 26 AleI CACnn'nnGTG Cuts at: 0 16 166 927 Size: 16 150 761 Fragments arranged by size: 761 150 16 AlwNI CAG_nnn'CTG Cuts at: 0 319 927 Size: 319 608 ApoI r'AATT_y Cuts at: 0 25 567 927 Size: 25 542 360 Fragments arranged by size: 542 360 25 AseI AT'TA_AT Cuts at: 0 599 927 Size: 599 328 BanI G'GyrC_C Cuts at: 0 478 927 Size: 478 449 BbsI GAAGACnn'nnnn_ Cuts at: 0 648 927 Size: 648 279 BclI T'GATC_A Cuts at: 0 134 927 Size: 134 793 Bme1580I G_kGCm'C Cuts at: 0 108 927 Size: 108 819 BmgBI CAC'GTC Cuts at: 0 654 927 Size: 654 273 BmrI ACTGGGnnnn_n' Cuts at: 0 534 927 Size: 534 393 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 247 277 399 429 927 Size: 247 30 122 30 498 Fragments arranged by size: 498 247 122 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 322 325 927 Size: 322 3 602 Fragments arranged by size: 602 322 3 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 927 Size: 367 560 BsiHKAI G_wGCw'C Cuts at: 0 412 927 Size: 412 515 BsmI GAATG_Cn' Cuts at: 0 72 927 Size: 72 855 Bsp1286I G_dGCh'C Cuts at: 0 108 412 927 Size: 108 304 515 Fragments arranged by size: 515 304 108 BspHI T'CATG_A Cuts at: 0 131 927 Size: 131 796 BsrDI GCAATG_nn' Cuts at: 0 758 927 Size: 758 169 BstXI CCAn_nnnn'nTGG Cuts at: 0 885 927 Size: 885 42 BstYI r'GATC_y Cuts at: 0 201 674 927 Size: 201 473 253 Fragments arranged by size: 473 253 201 EarI CTCTTCn'nnn_ Cuts at: 0 88 927 Size: 88 839 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 764 790 927 Size: 296 468 26 137 Fragments arranged by size: 468 296 137 26 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 530 927 Size: 530 397 MslI CAynn'nnrTG Cuts at: 0 16 130 166 343 389 883 927 Size: 16 114 36 177 46 494 44 Fragments arranged by size: 494 177 114 46 44 36 16 MspA1I CmG'CkG Cuts at: 0 475 927 Size: 475 452 PstI C_TGCA'G Cuts at: 0 324 927 Size: 324 603 PvuII CAG'CTG Cuts at: 0 475 927 Size: 475 452 SfcI C'TryA_G Cuts at: 0 320 662 927 Size: 320 342 265 Fragments arranged by size: 342 320 265 StyI C'CwwG_G Cuts at: 0 276 927 Size: 276 651 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 247 279 399 431 927 Size: 247 32 120 32 496 Fragments arranged by size: 496 247 120 32 32 Enzymes that do cut: AcuI AleI AlwNI ApoI AseI BanI BbsI BclI Bme1580I BmgBI BmrI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstXI BstYI EarI Eco57MI MmeI MslI MspA1I PstI PvuII SfcI StyI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI