Restriction map for
pF1KB7367
(Linear) (Six-base) MAPSORT of: KIBB7367.seq Check: 288 from: 1 to: 483
KIBB7367.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AccI GT'mk_AC
Cuts at: 0 47 89 483
Size: 47 42 394
Fragments arranged by size:
394 47 42
Acc65I G'GTAC_C
Cuts at: 0 74 483
Size: 74 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 483
Size: 304 179
AlwNI CAG_nnn'CTG
Cuts at: 0 342 483
Size: 342 141
ApaI G_GGCC'C
Cuts at: 0 185 254 291 483
Size: 185 69 37 192
Fragments arranged by size:
192 185 69 37
ApaLI G'TGCA_C
Cuts at: 0 205 483
Size: 205 278
BanI G'GyrC_C
Cuts at: 0 74 483
Size: 74 409
BanII G_rGCy'C
Cuts at: 0 185 254 291 483
Size: 185 69 37 192
Fragments arranged by size:
192 185 69 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 443 483
Size: 443 40
Bme1580I G_kGCm'C
Cuts at: 0 185 209 254 291 483
Size: 185 24 45 37 192
Fragments arranged by size:
192 185 45 37 24
Bpu10I CC'TnA_GC
Cuts at: 0 283 483
Size: 283 200
BsaI GGTCTCn'nnnn_
Cuts at: 0 386 472 483
Size: 386 86 11
Fragments arranged by size:
386 86 11
BsaAI yAC'GTr
Cuts at: 0 93 483
Size: 93 390
BsaHI Gr'CG_yC
Cuts at: 0 44 483
Size: 44 439
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 325 483
Size: 325 158
BsiHKAI G_wGCw'C
Cuts at: 0 200 209 483
Size: 200 9 274
Fragments arranged by size:
274 200 9
Bsp1286I G_dGCh'C
Cuts at: 0 185 200 209 254 291 483
Size: 185 15 9 45 37 192
Fragments arranged by size:
192 185 45 37 15 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 59 479 483
Size: 59 420 4
Fragments arranged by size:
420 59 4
BstAPI GCAn_nnn'nTGC
Cuts at: 0 342 483
Size: 342 141
BtgI C'CryG_G
Cuts at: 0 8 224 483
Size: 8 216 259
Fragments arranged by size:
259 216 8
BtsI GCAGTG_nn'
Cuts at: 0 110 379 483
Size: 110 269 104
Fragments arranged by size:
269 110 104
EaeI y'GGCC_r
Cuts at: 0 227 483
Size: 227 256
EarI CTCTTCn'nnn_
Cuts at: 0 239 446 483
Size: 239 207 37
Fragments arranged by size:
239 207 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 180 483
Size: 180 303
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 483
Size: 304 179
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 483
Size: 307 176
EcoO109I rG'GnC_Cy
Cuts at: 0 4 158 181 182 250 251 287 483
Size: 4 154 23 1 68 1 36 196
Fragments arranged by size:
196 154 68 36 23 4 1 1
HaeII r_GCGC'y
Cuts at: 0 36 483
Size: 36 447
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 383 415 483
Size: 383 32 68
Fragments arranged by size:
383 68 32
HincII GTy'rAC
Cuts at: 0 108 483
Size: 108 375
KpnI G_GTAC'C
Cuts at: 0 78 483
Size: 78 405
MspA1I CmG'CkG
Cuts at: 0 10 173 483
Size: 10 163 310
Fragments arranged by size:
310 163 10
PasI CC'CwG_GG
Cuts at: 0 185 483
Size: 185 298
PpuMI rG'GwC_Cy
Cuts at: 0 4 483
Size: 4 479
PspOMI G'GGCC_C
Cuts at: 0 181 250 287 483
Size: 181 69 37 196
Fragments arranged by size:
196 181 69 37
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 58 90 483
Size: 58 32 393
Fragments arranged by size:
393 58 32
SacII CC_GC'GG
Cuts at: 0 11 483
Size: 11 472
SanDI GG'GwC_CC
Cuts at: 0 4 483
Size: 4 479
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 133 483
Size: 133 350
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaI ApaLI BanI BanII
BbsI Bme1580I Bpu10I BsaI BsaAI BsaHI BseRI BsiHKAI
Bsp1286I BspMI BstAPI BtgI BtsI EaeI EarI EciI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MspA1I
PasI PpuMI PspOMI PsrI SacII SanDI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI
BpmI BpuEI BsaBI BsaWI BsaXI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI MfeI MluI MmeI MscI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI