Restriction map for
pF1KB7368
(Linear) (Six-base) MAPSORT of: KIBB7368.seq Check: 6990 from: 1 to: 948
KIBB7368.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 238 948
Size: 238 710
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 948
Size: 596 352
AleI CACnn'nnGTG
Cuts at: 0 743 948
Size: 743 205
ApoI r'AATT_y
Cuts at: 0 129 668 902 948
Size: 129 539 234 46
Fragments arranged by size:
539 234 129 46
AvrII C'CTAG_G
Cuts at: 0 627 948
Size: 627 321
BanII G_rGCy'C
Cuts at: 0 491 948
Size: 491 457
BbsI GAAGACnn'nnnn_
Cuts at: 0 15 741 898 948
Size: 15 726 157 50
Fragments arranged by size:
726 157 50 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 320 354 948
Size: 320 34 594
Fragments arranged by size:
594 320 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 355 397 948
Size: 355 42 551
Fragments arranged by size:
551 355 42
BfrBI ATG'CAT
Cuts at: 0 731 948
Size: 731 217
BglI GCCn_nnn'nGGC
Cuts at: 0 202 948
Size: 202 746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 948
Size: 85 863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 948
Size: 414 534
BseYI C'CCAG_C
Cuts at: 0 416 948
Size: 416 532
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 948
Size: 94 854
BsiHKAI G_wGCw'C
Cuts at: 0 551 948
Size: 551 397
BsmI GAATG_Cn'
Cuts at: 0 233 948
Size: 233 715
Bsp1286I G_dGCh'C
Cuts at: 0 491 551 948
Size: 491 60 397
Fragments arranged by size:
491 397 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 238 948
Size: 238 710
BsrDI GCAATG_nn'
Cuts at: 0 215 362 731 948
Size: 215 147 369 217
Fragments arranged by size:
369 217 215 147
BsrGI T'GTAC_A
Cuts at: 0 182 948
Size: 182 766
Bsu36I CC'TnA_GG
Cuts at: 0 483 948
Size: 483 465
BtsI GCAGTG_nn'
Cuts at: 0 82 948
Size: 82 866
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 198 233 948
Size: 198 35 715
Fragments arranged by size:
715 198 35
EaeI y'GGCC_r
Cuts at: 0 194 377 554 948
Size: 194 183 177 394
Fragments arranged by size:
394 194 183 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 596 948
Size: 85 511 352
Fragments arranged by size:
511 352 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 482 948
Size: 482 466
EcoRV GAT'ATC
Cuts at: 0 582 948
Size: 582 366
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 45 77 948
Size: 45 32 871
Fragments arranged by size:
871 45 32
HincII GTy'rAC
Cuts at: 0 591 948
Size: 591 357
MfeI C'AATT_G
Cuts at: 0 332 948
Size: 332 616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 948
Size: 61 887
MscI TGG'CCA
Cuts at: 0 196 379 556 948
Size: 196 183 177 392
Fragments arranged by size:
392 196 183 177
MslI CAynn'nnrTG
Cuts at: 0 743 948
Size: 743 205
MspA1I CmG'CkG
Cuts at: 0 690 948
Size: 690 258
NsiI A_TGCA'T
Cuts at: 0 733 948
Size: 733 215
NspI r_CATG'y
Cuts at: 0 403 731 948
Size: 403 328 217
Fragments arranged by size:
403 328 217
PsiI TTA'TAA
Cuts at: 0 148 472 649 948
Size: 148 324 177 299
Fragments arranged by size:
324 299 177 148
PvuII CAG'CTG
Cuts at: 0 690 948
Size: 690 258
SfcI C'TryA_G
Cuts at: 0 521 754 948
Size: 521 233 194
Fragments arranged by size:
521 233 194
SmlI C'TyrA_G
Cuts at: 0 393 948
Size: 393 555
SspI AAT'ATT
Cuts at: 0 644 948
Size: 644 304
StuI AGG'CCT
Cuts at: 0 718 948
Size: 718 230
StyI C'CwwG_G
Cuts at: 0 627 948
Size: 627 321
TatI w'GTAC_w
Cuts at: 0 182 948
Size: 182 766
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 431 463 948
Size: 431 32 485
Fragments arranged by size:
485 431 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 564 948
Size: 564 384
Enzymes that do cut:
AarI AcuI AleI ApoI AvrII BanII BbsI BcgI
BciVI BfrBI BglI BpmI BpuEI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrGI Bsu36I BtsI CspCI
EaeI Eco57MI EcoNI EcoRV Hin4I HincII MfeI MmeI
MscI MslI MspA1I NsiI NspI PsiI PvuII SfcI
SmlI SspI StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI BaeI BamHI BanI BbeI BbvCI BclI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI
ClaI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoO109I EcoRI FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI