(Linear) (Six-base) MAPSORT of: KIBB7370.seq  Check: 8032  from: 1  to: 591



KIBB7370.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     262     419     591 
   Size:         37     225     157     172

  Fragments arranged by size: 

                 225     172     157      37
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     219     591 
   Size:        219     372
 
ApoI r'AATT_y

Cuts at:      0     209     591 
   Size:        209     382
 
AvaI C'yCGr_G

Cuts at:      0     161     591 
   Size:        161     430
 
BanI G'GyrC_C

Cuts at:      0     389     591 
   Size:        389     202
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     242     359     591 
   Size:        242     117     232

  Fragments arranged by size: 

                 242     232     117
 
BglI GCCn_nnn'nGGC

Cuts at:      0     326     591 
   Size:        326     265
 
Bme1580I G_kGCm'C

Cuts at:      0     198     591 
   Size:        198     393
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513     591 
   Size:        513      78
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     591 
   Size:        300     291
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     494     524     591 
   Size:        494      30      67

  Fragments arranged by size: 

                 494      67      30
 
BseYI C'CCAG_C

Cuts at:      0     258     297     591 
   Size:        258      39     294

  Fragments arranged by size: 

                 294     258      39
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     591 
   Size:        307     284
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     247     591 
   Size:        247     344
 
Bsp1286I G_dGCh'C

Cuts at:      0     198     591 
   Size:        198     393
 
BsrBI CCG'CTC

Cuts at:      0     486     591 
   Size:        486     105
 
BstYI r'GATC_y

Cuts at:      0     526     591 
   Size:        526      65
 
BtgI C'CryG_G

Cuts at:      0      47     591 
   Size:         47     544
 
BtsI GCAGTG_nn'

Cuts at:      0     290     460     591 
   Size:        290     170     131

  Fragments arranged by size: 

                 290     170     131
 
EaeI y'GGCC_r

Cuts at:      0       2     591 
   Size:          2     589
 
EarI CTCTTCn'nnn_

Cuts at:      0      19     107     281     591 
   Size:         19      88     174     310

  Fragments arranged by size: 

                 310     174      88      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     262     419     513     591 
   Size:         37     225     157      94      78

  Fragments arranged by size: 

                 225     157      94      78      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0      12     591 
   Size:         12     579
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     360     449     591 
   Size:        360      89     142

  Fragments arranged by size: 

                 360     142      89
 
HindIII A'AGCT_T

Cuts at:      0     337     591 
   Size:        337     254
 
MslI CAynn'nnrTG

Cuts at:      0     201     591 
   Size:        201     390
 
NcoI C'CATG_G

Cuts at:      0      47     591 
   Size:         47     544
 
NspI r_CATG'y

Cuts at:      0     206     314     591 
   Size:        206     108     277

  Fragments arranged by size: 

                 277     206     108
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      48     591 
   Size:         48     543
 
PpuMI rG'GwC_Cy

Cuts at:      0      12     591 
   Size:         12     579
 
SapI GCTCTTCn'nnn_

Cuts at:      0     281     591 
   Size:        281     310
 
SfcI C'TryA_G

Cuts at:      0     143     591 
   Size:        143     448
 
SmlI C'TyrA_G

Cuts at:      0     315     591 
   Size:        315     276
 
SphI G_CATG'C

Cuts at:      0     206     591 
   Size:        206     385
 
StyI C'CwwG_G

Cuts at:      0      47     591 
   Size:         47     544
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      40     591 
   Size:         40     551
 
Tth111I GACn'n_nGTC

Cuts at:      0      10     591 
   Size:         10     581
 
XbaI T'CTAG_A

Cuts at:      0     348     591 
   Size:        348     243
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     AvaI     BanI     BbsI     BglI Bme1580I
     BpmI    BpuEI    BsaXI    BseYI     BsgI    BsmBI Bsp1286I    BsrBI
    BstYI     BtgI     BtsI     EaeI     EarI  Eco57MI EcoO109I     FalI
  HindIII     MslI     NcoI     NspI    PflMI    PpuMI     SapI     SfcI
     SmlI     SphI     StyI    TaqII  Tth111I     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TatI
     TstI     XcmI     XhoI     XmaI     XmnI     ZraI