(Linear) (Six-base) MAPSORT of: KIBB7373.seq  Check: 1066  from: 1  to: 744



KIBB7373.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     562     744 
   Size:        562     182
 
ApoI r'AATT_y

Cuts at:      0     617     744 
   Size:        617     127
 
BanII G_rGCy'C

Cuts at:      0     425     744 
   Size:        425     319
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      84     744 
   Size:         84     660
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      47     744 
   Size:         47     697
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     744 
   Size:         57     687
 
Bpu10I CC'TnA_GC

Cuts at:      0     419     744 
   Size:        419     325
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     147     744 
   Size:        147     597
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     519     744 
   Size:        519     225
 
BseYI C'CCAG_C

Cuts at:      0     699     744 
   Size:        699      45
 
Bsp1286I G_dGCh'C

Cuts at:      0     425     744 
   Size:        425     319
 
BtsI GCAGTG_nn'

Cuts at:      0     476     744 
   Size:        476     268
 
DraI TTT'AAA

Cuts at:      0     708     744 
   Size:        708      36
 
DraIII CAC_nnn'GTG

Cuts at:      0      55     744 
   Size:         55     689
 
EaeI y'GGCC_r

Cuts at:      0      93     744 
   Size:         93     651
 
EarI CTCTTCn'nnn_

Cuts at:      0     191     552     744 
   Size:        191     361     192

  Fragments arranged by size: 

                 361     192     191
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     744 
   Size:         57     687
 
EcoO109I rG'GnC_Cy

Cuts at:      0     343     744 
   Size:        343     401
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     701     744 
   Size:        701      43
 
MscI TGG'CCA

Cuts at:      0      95     744 
   Size:         95     649
 
MspA1I CmG'CkG

Cuts at:      0      99     744 
   Size:         99     645
 
PasI CC'CwG_GG

Cuts at:      0     199     744 
   Size:        199     545
 
PpuMI rG'GwC_Cy

Cuts at:      0     343     744 
   Size:        343     401
 
PvuII CAG'CTG

Cuts at:      0      99     744 
   Size:         99     645
 
SfcI C'TryA_G

Cuts at:      0     117     744 
   Size:        117     627
 
SmlI C'TyrA_G

Cuts at:      0     162     744 
   Size:        162     582
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      66     744 
   Size:         66     678
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     450     482     744 
   Size:        450      32     262

  Fragments arranged by size: 

                 450     262      32
 

 Enzymes that do cut:

    AlwNI     ApoI    BanII     BbsI     BmrI     BpmI   Bpu10I    BpuEI
    BseRI    BseYI Bsp1286I     BtsI     DraI   DraIII     EaeI     EarI
  Eco57MI EcoO109I     MmeI     MscI   MspA1I     PasI    PpuMI    PvuII
     SfcI     SmlI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI