(Linear) (Six-base) MAPSORT of: KIBB7374.seq  Check: 7241  from: 1  to: 678



KIBB7374.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AclI AA'CG_TT

Cuts at:      0     622     678 
   Size:        622      56
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     203     249     380     678 
   Size:         80     123      46     131     298

  Fragments arranged by size: 

                 298     131     123      80      46
 
AflII C'TTAA_G

Cuts at:      0     502     678 
   Size:        502     176
 
AlwNI CAG_nnn'CTG

Cuts at:      0      31     678 
   Size:         31     647
 
ApoI r'AATT_y

Cuts at:      0      75     177     484     678 
   Size:         75     102     307     194

  Fragments arranged by size: 

                 307     194     102      75
 
AseI AT'TA_AT

Cuts at:      0     397     678 
   Size:        397     281
 
AvrII C'CTAG_G

Cuts at:      0      10     678 
   Size:         10     668
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      74     678 
   Size:         74     604
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     543     678 
   Size:        543     135
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     678 
   Size:        469     209
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     310     678 
   Size:        310     368
 
BseYI C'CCAG_C

Cuts at:      0     238     678 
   Size:        238     440
 
BspHI T'CATG_A

Cuts at:      0     411     678 
   Size:        411     267
 
BtsI GCAGTG_nn'

Cuts at:      0     237     291     678 
   Size:        237      54     387

  Fragments arranged by size: 

                 387     237      54
 
EaeI y'GGCC_r

Cuts at:      0     391     678 
   Size:        391     287
 
EarI CTCTTCn'nnn_

Cuts at:      0     176     206     264     678 
   Size:        176      30      58     414

  Fragments arranged by size: 

                 414     176      58      30
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      80     203     249     380     678 
   Size:         80     123      46     131     298

  Fragments arranged by size: 

                 298     131     123      80      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     259     678 
   Size:        259     419
 
EcoRV GAT'ATC

Cuts at:      0     474     678 
   Size:        474     204
 
NspI r_CATG'y

Cuts at:      0     246     678 
   Size:        246     432
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     245     678 
   Size:        245     433
 
PstI C_TGCA'G

Cuts at:      0     306     678 
   Size:        306     372
 
SapI GCTCTTCn'nnn_

Cuts at:      0     206     678 
   Size:        206     472
 
SfcI C'TryA_G

Cuts at:      0     302     678 
   Size:        302     376
 
SmlI C'TyrA_G

Cuts at:      0     448     502     678 
   Size:        448      54     176

  Fragments arranged by size: 

                 448     176      54
 
SspI AAT'ATT

Cuts at:      0     401     678 
   Size:        401     277
 
StyI C'CwwG_G

Cuts at:      0      10     678 
   Size:         10     668
 

 Enzymes that do cut:

     AclI     AcuI    AflII    AlwNI     ApoI     AseI    AvrII     BbsI
     BplI    BpuEI    BseRI    BseYI    BspHI     BtsI     EaeI     EarI
  Eco57MI EcoO109I    EcoRV     NspI    PflMI     PstI     SapI     SfcI
     SmlI     SspI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI