Restriction map for
pF1KB7381
(Linear) (Six-base) MAPSORT of: KIBB7381.seq Check: 2645 from: 1 to: 1386
KIBB7381.orf
With 182 enzymes: SgfI *
March 3, 2008 11:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 672 688 1386
Size: 672 16 698
Fragments arranged by size:
698 672 16
AccI GT'mk_AC
Cuts at: 0 411 1386
Size: 411 975
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 909 1039 1386
Size: 571 338 130 347
Fragments arranged by size:
571 347 338 130
AflIII A'CryG_T
Cuts at: 0 227 1386
Size: 227 1159
AlwNI CAG_nnn'CTG
Cuts at: 0 339 1386
Size: 339 1047
ApaI G_GGCC'C
Cuts at: 0 792 941 1108 1257 1366 1386
Size: 792 149 167 149 109 20
Fragments arranged by size:
792 167 149 149 109 20
ApoI r'AATT_y
Cuts at: 0 758 1218 1386
Size: 758 460 168
Fragments arranged by size:
758 460 168
AvaI C'yCGr_G
Cuts at: 0 120 309 931 1249 1386
Size: 120 189 622 318 137
Fragments arranged by size:
622 318 189 137 120
BamHI G'GATC_C
Cuts at: 0 969 1386
Size: 969 417
BanI G'GyrC_C
Cuts at: 0 360 392 694 1386
Size: 360 32 302 692
Fragments arranged by size:
692 360 302 32
BanII G_rGCy'C
Cuts at: 0 121 479 652 792 870 941 1027 1108
Size: 121 358 173 140 78 71 86 81
Cuts at: 1108 1257 1366 1386
Size: 149 109 20
Fragments arranged by size:
358 173 149 140 121 109 86 81
78 71 20
BbeI G_GCGC'C
Cuts at: 0 396 1386
Size: 396 990
BbsI GAAGACnn'nnnn_
Cuts at: 0 820 1386
Size: 820 566
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 195 229 300 334 1386
Size: 195 34 71 34 1052
Fragments arranged by size:
1052 195 71 34 34
BglII A'GATC_T
Cuts at: 0 494 1386
Size: 494 892
Bme1580I G_kGCm'C
Cuts at: 0 365 699 792 941 1108 1257 1366 1386
Size: 365 334 93 149 167 149 109 20
Fragments arranged by size:
365 334 167 149 149 109 93 20
BmgBI CAC'GTC
Cuts at: 0 294 1386
Size: 294 1092
BmrI ACTGGGnnnn_n'
Cuts at: 0 174 427 1103 1386
Size: 174 253 676 283
Fragments arranged by size:
676 283 253 174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 666 1386
Size: 666 720
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 361 836 1386
Size: 135 226 475 550
Fragments arranged by size:
550 475 226 135
Bpu10I CC'TnA_GC
Cuts at: 0 274 478 768 1196 1386
Size: 274 204 290 428 190
Fragments arranged by size:
428 290 274 204 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1049 1386
Size: 1049 337
BsaHI Gr'CG_yC
Cuts at: 0 393 1386
Size: 393 993
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 730 760 1386
Size: 730 30 626
Fragments arranged by size:
730 626 30
BseYI C'CCAG_C
Cuts at: 0 40 1101 1293 1386
Size: 40 1061 192 93
Fragments arranged by size:
1061 192 93 40
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 466 916 1386
Size: 448 18 450 470
Fragments arranged by size:
470 450 448 18
BsiEI CG_ry'CG
Cuts at: 0 327 387 1386
Size: 327 60 999
Fragments arranged by size:
999 327 60
BsiHKAI G_wGCw'C
Cuts at: 0 457 479 652 1386
Size: 457 22 173 734
Fragments arranged by size:
734 457 173 22
BsmI GAATG_Cn'
Cuts at: 0 951 1386
Size: 951 435
Bsp1286I G_dGCh'C
Cuts at: 0 121 365 457 479 652 699 792 870
Size: 121 244 92 22 173 47 93 78
Cuts at: 870 941 1027 1108 1257 1366 1386
Size: 71 86 81 149 109 20
Fragments arranged by size:
244 173 149 121 109 93 92 86
81 78 71 47 22 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 672 688 1089 1386
Size: 672 16 401 297
Fragments arranged by size:
672 401 297 16
BsrBI CCG'CTC
Cuts at: 0 125 1185 1386
Size: 125 1060 201
Fragments arranged by size:
1060 201 125
BsrDI GCAATG_nn'
Cuts at: 0 609 1386
Size: 609 777
BstAPI GCAn_nnn'nTGC
Cuts at: 0 435 1386
Size: 435 951
BstYI r'GATC_y
Cuts at: 0 494 969 1386
Size: 494 475 417
Fragments arranged by size:
494 475 417
Bsu36I CC'TnA_GG
Cuts at: 0 829 1386
Size: 829 557
BtgI C'CryG_G
Cuts at: 0 26 531 1386
Size: 26 505 855
Fragments arranged by size:
855 505 26
BtsI GCAGTG_nn'
Cuts at: 0 877 1386
Size: 877 509
EaeI y'GGCC_r
Cuts at: 0 324 384 1386
Size: 324 60 1002
Fragments arranged by size:
1002 324 60
EagI C'GGCC_G
Cuts at: 0 324 384 1386
Size: 324 60 1002
Fragments arranged by size:
1002 324 60
EcoICRI GAG'CTC
Cuts at: 0 477 650 1386
Size: 477 173 736
Fragments arranged by size:
736 477 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 361 571 836 909 1039 1386
Size: 135 226 210 265 73 130 347
Fragments arranged by size:
347 265 226 210 135 130 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 703 962 1386
Size: 703 259 424
Fragments arranged by size:
703 424 259
EcoO109I rG'GnC_Cy
Cuts at: 0 37 87 402 708 788 789 937 994
Size: 37 50 315 306 80 1 148 57
Cuts at: 994 1087 1105 1239 1253 1254 1386
Size: 93 18 134 14 1 132
Fragments arranged by size:
315 306 148 134 132 93 80 57
50 37 18 14 1 1
HaeII r_GCGC'y
Cuts at: 0 13 79 396 1386
Size: 13 66 317 990
Fragments arranged by size:
990 317 66 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 519 551 555 587 807 839 1386
Size: 519 32 4 32 220 32 547
Fragments arranged by size:
547 519 220 32 32 32 4
HincII GTy'rAC
Cuts at: 0 412 1386
Size: 412 974
KasI G'GCGC_C
Cuts at: 0 392 1386
Size: 392 994
MspA1I CmG'CkG
Cuts at: 0 16 113 389 1386
Size: 16 97 276 997
Fragments arranged by size:
997 276 97 16
NarI GG'CG_CC
Cuts at: 0 393 1386
Size: 393 993
NotI GC'GGCC_GC
Cuts at: 0 324 1386
Size: 324 1062
NspI r_CATG'y
Cuts at: 0 231 1386
Size: 231 1155
PasI CC'CwG_GG
Cuts at: 0 974 1231 1299 1386
Size: 974 257 68 87
Fragments arranged by size:
974 257 87 68
PciI A'CATG_T
Cuts at: 0 227 1386
Size: 227 1159
PfoI T'CCnGG_A
Cuts at: 0 905 1386
Size: 905 481
PpuMI rG'GwC_Cy
Cuts at: 0 87 402 708 1239 1386
Size: 87 315 306 531 147
Fragments arranged by size:
531 315 306 147 87
PshAI GACnn'nnGTC
Cuts at: 0 407 1386
Size: 407 979
PspOMI G'GGCC_C
Cuts at: 0 788 937 1104 1253 1362 1386
Size: 788 149 167 149 109 24
Fragments arranged by size:
788 167 149 149 109 24
PstI C_TGCA'G
Cuts at: 0 707 1086 1386
Size: 707 379 300
Fragments arranged by size:
707 379 300
PvuII CAG'CTG
Cuts at: 0 16 1386
Size: 16 1370
SacI G_AGCT'C
Cuts at: 0 479 652 1386
Size: 479 173 734
Fragments arranged by size:
734 479 173
SalI G'TCGA_C
Cuts at: 0 410 1386
Size: 410 976
SanDI GG'GwC_CC
Cuts at: 0 87 1386
Size: 87 1299
SbfI CC_TGCA'GG
Cuts at: 0 1086 1386
Size: 1086 300
SexAI A'CCwGG_T
Cuts at: 0 690 1386
Size: 690 696
SfcI C'TryA_G
Cuts at: 0 703 1082 1386
Size: 703 379 304
Fragments arranged by size:
703 379 304
SfoI GGC'GCC
Cuts at: 0 394 1386
Size: 394 992
SmlI C'TyrA_G
Cuts at: 0 1064 1386
Size: 1064 322
StyI C'CwwG_G
Cuts at: 0 164 1116 1172 1283 1386
Size: 164 952 56 111 103
Fragments arranged by size:
952 164 111 103 56
Tth111I GACn'n_nGTC
Cuts at: 0 292 1386
Size: 292 1094
XbaI T'CTAG_A
Cuts at: 0 1092 1386
Size: 1092 294
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 574 1386
Size: 574 812
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BcgI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BstAPI BstYI Bsu36I BtgI BtsI EaeI EagI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MspA1I NarI NotI NspI PasI PciI PfoI PpuMI
PshAI PspOMI PstI PvuII SacI SalI SanDI SbfI
SexAI SfcI SfoI SmlI StyI Tth111I XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BciVI BclI BfrBI BglI BlpI BmtI BsaI
BsaAI BsaBI BsaWI BseRI BsiWI BsmBI BspEI BspHI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EciI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NaeI NcoI
NdeI NgoMIV NheI NruI NsiI PacI PflMI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII
SapI ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII TatI TstI
XhoI XmaI XmnI ZraI