(Linear) (Six-base) MAPSORT of: KIBB7382.seq Check: 8056 from: 1 to: 939 KIBB7382.orf With 182 enzymes: SgfI * March 3, 2008 11:00 .. AccI GT'mk_AC Cuts at: 0 423 495 712 939 Size: 423 72 217 227 Fragments arranged by size: 423 227 217 72 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 710 939 Size: 710 229 AhdI GACnn_n'nnGTC Cuts at: 0 491 939 Size: 491 448 ApoI r'AATT_y Cuts at: 0 31 939 Size: 31 908 BanI G'GyrC_C Cuts at: 0 695 939 Size: 695 244 BbeI G_GCGC'C Cuts at: 0 699 939 Size: 699 240 BbsI GAAGACnn'nnnn_ Cuts at: 0 30 810 939 Size: 30 780 129 Fragments arranged by size: 780 129 30 BclI T'GATC_A Cuts at: 0 344 939 Size: 344 595 BglII A'GATC_T Cuts at: 0 332 939 Size: 332 607 Bpu10I CC'TnA_GC Cuts at: 0 187 901 939 Size: 187 714 38 Fragments arranged by size: 714 187 38 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 885 939 Size: 885 54 BsaAI yAC'GTr Cuts at: 0 369 939 Size: 369 570 BsaHI Gr'CG_yC Cuts at: 0 696 939 Size: 696 243 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 889 939 Size: 889 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 363 549 939 Size: 363 186 390 Fragments arranged by size: 390 363 186 BsmI GAATG_Cn' Cuts at: 0 88 939 Size: 88 851 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 724 939 Size: 724 215 BsrDI GCAATG_nn' Cuts at: 0 137 939 Size: 137 802 BstBI TT'CG_AA Cuts at: 0 675 939 Size: 675 264 BstEII G'GTnAC_C Cuts at: 0 642 831 939 Size: 642 189 108 Fragments arranged by size: 642 189 108 BstYI r'GATC_y Cuts at: 0 332 939 Size: 332 607 BstZ17I GTA'TAC Cuts at: 0 424 939 Size: 424 515 BtgI C'CryG_G Cuts at: 0 638 939 Size: 638 301 EaeI y'GGCC_r Cuts at: 0 635 778 939 Size: 635 143 161 Fragments arranged by size: 635 161 143 EarI CTCTTCn'nnn_ Cuts at: 0 307 677 751 939 Size: 307 370 74 188 Fragments arranged by size: 370 307 188 74 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 710 939 Size: 710 229 HaeII r_GCGC'y Cuts at: 0 699 939 Size: 699 240 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 477 509 939 Size: 477 32 430 Fragments arranged by size: 477 430 32 KasI G'GCGC_C Cuts at: 0 695 939 Size: 695 244 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 224 939 Size: 224 715 MslI CAynn'nnrTG Cuts at: 0 103 939 Size: 103 836 MspA1I CmG'CkG Cuts at: 0 688 939 Size: 688 251 NarI GG'CG_CC Cuts at: 0 696 939 Size: 696 243 PflMI CCAn_nnn'nTGG Cuts at: 0 732 939 Size: 732 207 PvuII CAG'CTG Cuts at: 0 688 939 Size: 688 251 SapI GCTCTTCn'nnn_ Cuts at: 0 307 939 Size: 307 632 SfoI GGC'GCC Cuts at: 0 697 939 Size: 697 242 SmlI C'TyrA_G Cuts at: 0 864 939 Size: 864 75 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 804 939 Size: 804 135 XmnI GAAnn'nnTTC Cuts at: 0 850 939 Size: 850 89 Enzymes that do cut: AccI AcuI AhdI ApoI BanI BbeI BbsI BclI BglII Bpu10I BpuEI BsaAI BsaHI BseRI BsgI BsmI BspMI BsrDI BstBI BstEII BstYI BstZ17I BtgI EaeI EarI Eco57MI HaeII Hin4I KasI MmeI MslI MspA1I NarI PflMI PvuII SapI SfoI SmlI XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanII BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BsaI BsaBI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstXI Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI ZraI