(Linear) (Six-base) MAPSORT of: KIBB7393.seq  Check: 4882  from: 1  to: 1242



KIBB7393.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:00  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     368     460    1196    1242 
   Size:        175     193      92     736      46

  Fragments arranged by size: 

                 736     193     175      92      46
 
AflII C'TTAA_G

Cuts at:      0     751    1242 
   Size:        751     491
 
AleI CACnn'nnGTG

Cuts at:      0     373    1242 
   Size:        373     869
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1151    1242 
   Size:       1151      91
 
ApoI r'AATT_y

Cuts at:      0     214     388     450     756     868     876    1064    1242 
   Size:        214     174      62     306     112       8     188     178

  Fragments arranged by size: 

                 306     214     188     178     174     112      62       8
            
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     341     374    1242 
   Size:        341      33     868

  Fragments arranged by size: 

                 868     341      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     385    1242 
   Size:        385     857
 
BbvCI CC'TCA_GC

Cuts at:      0     697    1242 
   Size:        697     545
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1047    1242 
   Size:       1047     195
 
BclI T'GATC_A

Cuts at:      0      26    1242 
   Size:         26    1216
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     344    1242 
   Size:        344     898
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     655     727    1242 
   Size:        655      72     515

  Fragments arranged by size: 

                 655     515      72
 
Bpu10I CC'TnA_GC

Cuts at:      0      55     184     697    1242 
   Size:         55     129     513     545

  Fragments arranged by size: 

                 545     513     129      55
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     570    1242 
   Size:        570     672
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     584    1242 
   Size:        584     658
 
BsaHI Gr'CG_yC

Cuts at:      0      74    1242 
   Size:         74    1168
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     748     965    1242 
   Size:        748     217     277

  Fragments arranged by size: 

                 748     277     217
 
BsmI GAATG_Cn'

Cuts at:      0    1128    1242 
   Size:       1128     114
 
BsrFI r'CCGG_y

Cuts at:      0      32     123    1242 
   Size:         32      91    1119

  Fragments arranged by size: 

                1119      91      32
 
BstYI r'GATC_y

Cuts at:      0     897    1242 
   Size:        897     345
 
Bsu36I CC'TnA_GG

Cuts at:      0     535    1242 
   Size:        535     707
 
DraI TTT'AAA

Cuts at:      0     299     402     761    1242 
   Size:        299     103     359     481

  Fragments arranged by size: 

                 481     359     299     103
 
DraIII CAC_nnn'GTG

Cuts at:      0    1146    1242 
   Size:       1146      96
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     368     460     655     727    1196    1242 
   Size:        175     193      92     195      72     469      46

  Fragments arranged by size: 

                 469     195     193     175      92      72      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     170    1242 
   Size:        170    1072
 
EcoRV GAT'ATC

Cuts at:      0     267    1242 
   Size:        267     975
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     325    1242 
   Size:        325     917
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      81     113    1242 
   Size:         81      32    1129

  Fragments arranged by size: 

                1129      81      32
 
HindIII A'AGCT_T

Cuts at:      0     571     847    1242 
   Size:        571     276     395

  Fragments arranged by size: 

                 571     395     276
 
MfeI C'AATT_G

Cuts at:      0     584    1242 
   Size:        584     658
 
MslI CAynn'nnrTG

Cuts at:      0     373    1242 
   Size:        373     869
 
NaeI GCC'GGC

Cuts at:      0      34    1242 
   Size:         34    1208
 
NdeI CA'TA_TG

Cuts at:      0    1011    1242 
   Size:       1011     231
 
NgoMIV G'CCGG_C

Cuts at:      0      32    1242 
   Size:         32    1210
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1050    1146    1242 
   Size:       1050      96      96

  Fragments arranged by size: 

                1050      96      96
 
PfoI T'CCnGG_A

Cuts at:      0     109     743    1242 
   Size:        109     634     499

  Fragments arranged by size: 

                 634     499     109
 
PpuMI rG'GwC_Cy

Cuts at:      0     170    1242 
   Size:        170    1072
 
PshAI GACnn'nnGTC

Cuts at:      0     551    1242 
   Size:        551     691
 
RsrII CG'GwC_CG

Cuts at:      0     126    1242 
   Size:        126    1116
 
SanDI GG'GwC_CC

Cuts at:      0     170    1242 
   Size:        170    1072
 
SmlI C'TyrA_G

Cuts at:      0     549     751    1242 
   Size:        549     202     491

  Fragments arranged by size: 

                 549     491     202
 
StyI C'CwwG_G

Cuts at:      0      47     464    1242 
   Size:         47     417     778

  Fragments arranged by size: 

                 778     417      47
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1156    1242 
   Size:       1156      86
 
XmnI GAAnn'nnTTC

Cuts at:      0     473     782    1096    1242 
   Size:        473     309     314     146

  Fragments arranged by size: 

                 473     314     309     146
 

 Enzymes that do cut:

     AcuI    AflII     AleI     AlfI     ApoI     BaeI     BbsI    BbvCI
    BciVI     BclI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI
     BsgI     BsmI    BsrFI    BstYI   Bsu36I     DraI   DraIII  Eco57MI
 EcoO109I    EcoRV     FalI    Hin4I  HindIII     MfeI     MslI     NaeI
     NdeI   NgoMIV    PflMI     PfoI    PpuMI    PshAI    RsrII    SanDI
     SmlI     StyI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BcgI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI     FseI     FspI    FspAI    HaeII
   HincII     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I
     NarI     NcoI     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI