(Linear) (Six-base) MAPSORT of: KIBB7397.seq  Check: 4019  from: 1  to: 591



KIBB7397.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     193     591 
   Size:        193     398
 
AflIII A'CryG_T

Cuts at:      0     389     591 
   Size:        389     202
 
AlwNI CAG_nnn'CTG

Cuts at:      0     432     576     591 
   Size:        432     144      15

  Fragments arranged by size: 

                 432     144      15
 
ApoI r'AATT_y

Cuts at:      0     117     591 
   Size:        117     474
 
BanI G'GyrC_C

Cuts at:      0     102     591 
   Size:        102     489
 
BbeI G_GCGC'C

Cuts at:      0     106     591 
   Size:        106     485
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     530     591 
   Size:        530      61
 
BbvCI CC'TCA_GC

Cuts at:      0     363     591 
   Size:        363     228
 
BclI T'GATC_A

Cuts at:      0     500     591 
   Size:        500      91
 
BglII A'GATC_T

Cuts at:      0      74     591 
   Size:         74     517
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     591 
   Size:        454     137
 
Bpu10I CC'TnA_GC

Cuts at:      0     363     591 
   Size:        363     228
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      60     591 
   Size:         60     531
 
BsaAI yAC'GTr

Cuts at:      0     392     591 
   Size:        392     199
 
BsaHI Gr'CG_yC

Cuts at:      0     103     591 
   Size:        103     488
 
BsaWI w'CCGG_w

Cuts at:      0      13     591 
   Size:         13     578
 
BsiWI C'GTAC_G

Cuts at:      0     392     591 
   Size:        392     199
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     402     591 
   Size:        402     189
 
BsrGI T'GTAC_A

Cuts at:      0     303     591 
   Size:        303     288
 
BssHII G'CGCG_C

Cuts at:      0      41     591 
   Size:         41     550
 
BstEII G'GTnAC_C

Cuts at:      0     216     591 
   Size:        216     375
 
BstYI r'GATC_y

Cuts at:      0      74     591 
   Size:         74     517
 
BstZ17I GTA'TAC

Cuts at:      0     194     591 
   Size:        194     397
 
BtgI C'CryG_G

Cuts at:      0     369     512     591 
   Size:        369     143      79

  Fragments arranged by size: 

                 369     143      79
 
EarI CTCTTCn'nnn_

Cuts at:      0     342     591 
   Size:        342     249
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454     591 
   Size:        454     137
 
HaeII r_GCGC'y

Cuts at:      0     106     591 
   Size:        106     485
 
HincII GTy'rAC

Cuts at:      0     420     591 
   Size:        420     171
 
KasI G'GCGC_C

Cuts at:      0     102     591 
   Size:        102     489
 
MslI CAynn'nnrTG

Cuts at:      0     187     534     591 
   Size:        187     347      57

  Fragments arranged by size: 

                 347     187      57
 
NarI GG'CG_CC

Cuts at:      0     103     591 
   Size:        103     488
 
NcoI C'CATG_G

Cuts at:      0     369     512     591 
   Size:        369     143      79

  Fragments arranged by size: 

                 369     143      79
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     432     591 
   Size:        432     159
 
PstI C_TGCA'G

Cuts at:      0      50     591 
   Size:         50     541
 
SfcI C'TryA_G

Cuts at:      0      46     591 
   Size:         46     545
 
SfoI GGC'GCC

Cuts at:      0     104     591 
   Size:        104     487
 
StyI C'CwwG_G

Cuts at:      0      87     369     512     591 
   Size:         87     282     143      79

  Fragments arranged by size: 

                 282     143      87      79
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     299     591 
   Size:        299     292
 
TatI w'GTAC_w

Cuts at:      0     167     303     591 
   Size:        167     136     288

  Fragments arranged by size: 

                 288     167     136
 
XmnI GAAnn'nnTTC

Cuts at:      0     229     591 
   Size:        229     362
 

 Enzymes that do cut:

     AccI   AflIII    AlwNI     ApoI     BanI     BbeI     BbsI    BbvCI
     BclI    BglII     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstYI  BstZ17I     BtgI
     EarI  Eco57MI    HaeII   HincII     KasI     MslI     NarI     NcoI
    PflMI     PstI     SfcI     SfoI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BcgI    BciVI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
    BpuEI    BsaBI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
   MspA1I     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI