Restriction map for
pF1KB7398
(Linear) (Six-base) MAPSORT of: KIBB7398.seq Check: 4031 from: 1 to: 975
KIBB7398.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 273 975
Size: 273 702
Acc65I G'GTAC_C
Cuts at: 0 628 739 975
Size: 628 111 236
Fragments arranged by size:
628 236 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 416 975
Size: 250 166 559
Fragments arranged by size:
559 250 166
AfeI AGC'GCT
Cuts at: 0 389 975
Size: 389 586
AhdI GACnn_n'nnGTC
Cuts at: 0 201 519 975
Size: 201 318 456
Fragments arranged by size:
456 318 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 39 975
Size: 39 936
AseI AT'TA_AT
Cuts at: 0 758 791 824 975
Size: 758 33 33 151
Fragments arranged by size:
758 151 33 33
AvrII C'CTAG_G
Cuts at: 0 199 958 975
Size: 199 759 17
Fragments arranged by size:
759 199 17
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 855 888 975
Size: 855 33 87
Fragments arranged by size:
855 87 33
BamHI G'GATC_C
Cuts at: 0 339 975
Size: 339 636
BanI G'GyrC_C
Cuts at: 0 628 739 975
Size: 628 111 236
Fragments arranged by size:
628 236 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 46 455 975
Size: 46 409 520
Fragments arranged by size:
520 409 46
BbvCI CC'TCA_GC
Cuts at: 0 134 368 975
Size: 134 234 607
Fragments arranged by size:
607 234 134
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 675 709 975
Size: 675 34 266
Fragments arranged by size:
675 266 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 776 809 842 975
Size: 776 33 33 133
Fragments arranged by size:
776 133 33 33
BclI T'GATC_A
Cuts at: 0 615 975
Size: 615 360
BglI GCCn_nnn'nGGC
Cuts at: 0 911 975
Size: 911 64
BlpI GC'TnA_GC
Cuts at: 0 235 975
Size: 235 740
BmrI ACTGGGnnnn_n'
Cuts at: 0 559 635 975
Size: 559 76 340
Fragments arranged by size:
559 340 76
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 44 311 975
Size: 44 267 664
Fragments arranged by size:
664 267 44
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 914 975
Size: 399 515 61
Fragments arranged by size:
515 399 61
Bpu10I CC'TnA_GC
Cuts at: 0 134 368 975
Size: 134 234 607
Fragments arranged by size:
607 234 134
BsaI GGTCTCn'nnnn_
Cuts at: 0 596 975
Size: 596 379
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 72 102 975
Size: 72 30 873
Fragments arranged by size:
873 72 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 20 112 493 975
Size: 20 92 381 482
Fragments arranged by size:
482 381 92 20
BseYI C'CCAG_C
Cuts at: 0 746 779 812 845 930 975
Size: 746 33 33 33 85 45
Fragments arranged by size:
746 85 45 33 33 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 975
Size: 304 671
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 273 975
Size: 273 702
BsrBI CCG'CTC
Cuts at: 0 190 975
Size: 190 785
BstYI r'GATC_y
Cuts at: 0 339 975
Size: 339 636
Bsu36I CC'TnA_GG
Cuts at: 0 335 704 975
Size: 335 369 271
Fragments arranged by size:
369 335 271
BtgI C'CryG_G
Cuts at: 0 317 975
Size: 317 658
BtsI GCAGTG_nn'
Cuts at: 0 287 447 973 975
Size: 287 160 526 2
Fragments arranged by size:
526 287 160 2
EarI CTCTTCn'nnn_
Cuts at: 0 921 975
Size: 921 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 892 975
Size: 892 83
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 399 416 914 975
Size: 250 149 17 498 61
Fragments arranged by size:
498 250 149 61 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 269 508 975
Size: 269 239 467
Fragments arranged by size:
467 269 239
EcoO109I rG'GnC_Cy
Cuts at: 0 664 975
Size: 664 311
HaeII r_GCGC'y
Cuts at: 0 391 975
Size: 391 584
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 505 537 975
Size: 187 32 286 32 438
Fragments arranged by size:
438 286 187 32 32
KpnI G_GTAC'C
Cuts at: 0 632 743 975
Size: 632 111 232
Fragments arranged by size:
632 232 111
MspA1I CmG'CkG
Cuts at: 0 746 779 812 845 934 975
Size: 746 33 33 33 89 41
Fragments arranged by size:
746 89 41 33 33 33
NcoI C'CATG_G
Cuts at: 0 317 975
Size: 317 658
PasI CC'CwG_GG
Cuts at: 0 623 667 975
Size: 623 44 308
Fragments arranged by size:
623 308 44
PflMI CCAn_nnn'nTGG
Cuts at: 0 117 975
Size: 117 858
PfoI T'CCnGG_A
Cuts at: 0 768 801 834 891 975
Size: 768 33 33 57 84
Fragments arranged by size:
768 84 57 33 33
PstI C_TGCA'G
Cuts at: 0 270 282 975
Size: 270 12 693
Fragments arranged by size:
693 270 12
PvuII CAG'CTG
Cuts at: 0 746 779 812 845 934 975
Size: 746 33 33 33 89 41
Fragments arranged by size:
746 89 41 33 33 33
SapI GCTCTTCn'nnn_
Cuts at: 0 921 975
Size: 921 54
SbfI CC_TGCA'GG
Cuts at: 0 270 975
Size: 270 705
SfcI C'TryA_G
Cuts at: 0 266 278 975
Size: 266 12 697
Fragments arranged by size:
697 266 12
SspI AAT'ATT
Cuts at: 0 756 789 822 855 975
Size: 756 33 33 33 120
Fragments arranged by size:
756 120 33 33 33
StuI AGG'CCT
Cuts at: 0 68 482 975
Size: 68 414 493
Fragments arranged by size:
493 414 68
StyI C'CwwG_G
Cuts at: 0 199 317 343 491 958 975
Size: 199 118 26 148 467 17
Fragments arranged by size:
467 199 148 118 26 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 504 975
Size: 504 471
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 72 104 975
Size: 72 32 871
Fragments arranged by size:
871 72 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 743 776 809 842 975
Size: 743 33 33 33 133
Fragments arranged by size:
743 133 33 33 33
Enzymes that do cut:
AarI Acc65I AcuI AfeI AhdI AlfI AseI AvrII
BaeI BamHI BanI BbsI BbvCI BcgI BciVI BclI
BglI BlpI BmrI BplI BpmI Bpu10I BsaI BsaXI
BseRI BseYI BsgI BspMI BsrBI BstYI Bsu36I BtgI
BtsI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
KpnI MspA1I NcoI PasI PflMI PfoI PstI PvuII
SapI SbfI SfcI SspI StuI StyI TaqII TstI
XcmI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AleI AloI
AlwNI ApaI ApaLI ApoI AscI AsiSI AvaI BanII
BbeI BfrBI BglII Bme1580I BmgBI BmtI BpuEI BsaAI
BsaBI BsaHI BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI
Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EaeI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
MfeI MluI MmeI MscI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI TatI Tth111I XbaI XhoI
XmaI XmnI ZraI