(Linear) (Six-base) MAPSORT of: KIBB7399.seq  Check: 3899  from: 1  to: 528



KIBB7399.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     363     528 
   Size:        363     165
 
BanII G_rGCy'C

Cuts at:      0     505     528 
   Size:        505      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     165     280     528 
   Size:        165     115     248

  Fragments arranged by size: 

                 248     165     115
 
BlpI GC'TnA_GC

Cuts at:      0      16      28     528 
   Size:         16      12     500

  Fragments arranged by size: 

                 500      16      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     528 
   Size:        355     173
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     155     388     528 
   Size:        155     233     140

  Fragments arranged by size: 

                 233     155     140
 
BsaWI w'CCGG_w

Cuts at:      0     179     528 
   Size:        179     349
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     528 
   Size:        256     272
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     336     528 
   Size:        336     192
 
Bsp1286I G_dGCh'C

Cuts at:      0     505     528 
   Size:        505      23
 
BspEI T'CCGG_A

Cuts at:      0     179     528 
   Size:        179     349
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     430     528 
   Size:        430      98
 
BstBI TT'CG_AA

Cuts at:      0     224     528 
   Size:        224     304
 
BstYI r'GATC_y

Cuts at:      0     474     528 
   Size:        474      54
 
Bsu36I CC'TnA_GG

Cuts at:      0     166     528 
   Size:        166     362
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      21     528 
   Size:         21     507
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     169     528 
   Size:        169     359
 
EaeI y'GGCC_r

Cuts at:      0      89     326     528 
   Size:         89     237     202

  Fragments arranged by size: 

                 237     202      89
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     363     528 
   Size:        355       8     165

  Fragments arranged by size: 

                 355     165       8
 
HaeII r_GCGC'y

Cuts at:      0     412     528 
   Size:        412     116
 
MscI TGG'CCA

Cuts at:      0      91     528 
   Size:         91     437
 
MslI CAynn'nnrTG

Cuts at:      0      67     528 
   Size:         67     461
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     405     524     528 
   Size:        405     119       4

  Fragments arranged by size: 

                 405     119       4
 
SfcI C'TryA_G

Cuts at:      0     345     528 
   Size:        345     183
 
SspI AAT'ATT

Cuts at:      0     186     215     528 
   Size:        186      29     313

  Fragments arranged by size: 

                 313     186      29
 
StuI AGG'CCT

Cuts at:      0     513     528 
   Size:        513      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     406     528 
   Size:        406     122
 

 Enzymes that do cut:

     AcuI    BanII     BbsI     BlpI     BpmI     BsaI    BsaWI     BsgI
    BsmBI Bsp1286I    BspEI    BspMI    BstBI    BstYI   Bsu36I    BtgZI
     DrdI     EaeI  Eco57MI    HaeII     MscI     MslI    PflMI     SfcI
     SspI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI     BsmI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI   BstEII    BstXI  BstZ17I     BtgI     BtsI     ClaI
    CspCI     DraI   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI