(Linear) (Six-base) MAPSORT of: KIBB7400.seq  Check: 3941  from: 1  to: 915



KIBB7400.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     224     915 
   Size:        224     691
 
AclI AA'CG_TT

Cuts at:      0     484     915 
   Size:        484     431
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     915 
   Size:        278     637
 
AlwNI CAG_nnn'CTG

Cuts at:      0      25     915 
   Size:         25     890
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     266     915 
   Size:        266     649
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     114     148     915 
   Size:        114      34     767

  Fragments arranged by size: 

                 767     114      34
 
BglII A'GATC_T

Cuts at:      0     784     915 
   Size:        784     131
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     693     915 
   Size:        693     222
 
BsaAI yAC'GTr

Cuts at:      0     366     915 
   Size:        366     549
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     739     769     915 
   Size:        739      30     146

  Fragments arranged by size: 

                 739     146      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      21     672     675     711     915 
   Size:         21     651       3      36     204

  Fragments arranged by size: 

                 651     204      36      21       3
 
BseYI C'CCAG_C

Cuts at:      0      39      69     915 
   Size:         39      30     846

  Fragments arranged by size: 

                 846      39      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     635     915 
   Size:        635     280
 
BsmI GAATG_Cn'

Cuts at:      0      68     915 
   Size:         68     847
 
Bsp1286I G_dGCh'C

Cuts at:      0     635     915 
   Size:        635     280
 
BspHI T'CATG_A

Cuts at:      0     857     915 
   Size:        857      58
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     256     344     915 
   Size:        256      88     571

  Fragments arranged by size: 

                 571     256      88
 
BsrDI GCAATG_nn'

Cuts at:      0     421     915 
   Size:        421     494
 
BstYI r'GATC_y

Cuts at:      0     204     236     677     784     915 
   Size:        204      32     441     107     131

  Fragments arranged by size: 

                 441     204     131     107      32
 
Bsu36I CC'TnA_GG

Cuts at:      0     871     915 
   Size:        871      44
 
BtgI C'CryG_G

Cuts at:      0     347     915 
   Size:        347     568
 
BtsI GCAGTG_nn'

Cuts at:      0     635     915 
   Size:        635     280
 
EaeI y'GGCC_r

Cuts at:      0     368     915 
   Size:        368     547
 
EarI CTCTTCn'nnn_

Cuts at:      0      82     468     915 
   Size:         82     386     447

  Fragments arranged by size: 

                 447     386      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     278     915 
   Size:        278     637
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      29     915 
   Size:         29     886
 
MscI TGG'CCA

Cuts at:      0     370     915 
   Size:        370     545
 
MslI CAynn'nnrTG

Cuts at:      0     169     915 
   Size:        169     746
 
MspA1I CmG'CkG

Cuts at:      0      69     915 
   Size:         69     846
 
NspI r_CATG'y

Cuts at:      0     719     915 
   Size:        719     196
 
PshAI GACnn'nnGTC

Cuts at:      0     736     915 
   Size:        736     179
 
PstI C_TGCA'G

Cuts at:      0     312     915 
   Size:        312     603
 
PvuII CAG'CTG

Cuts at:      0      69     915 
   Size:         69     846
 
SfcI C'TryA_G

Cuts at:      0     308     321     864     915 
   Size:        308      13     543      51

  Fragments arranged by size: 

                 543     308      51      13
 
SmlI C'TyrA_G

Cuts at:      0     672     915 
   Size:        672     243
 
StyI C'CwwG_G

Cuts at:      0     545     689     915 
   Size:        545     144     226

  Fragments arranged by size: 

                 545     226     144
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     183     915 
   Size:        183     732
 
TatI w'GTAC_w

Cuts at:      0      98     593     915 
   Size:         98     495     322

  Fragments arranged by size: 

                 495     322      98
 

 Enzymes that do cut:

     AccI     AclI     AcuI    AlwNI     BbsI     BcgI    BglII    BpuEI
    BsaAI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI    BstYI   Bsu36I     BtgI     BtsI     EaeI     EarI
  Eco57MI    Hin4I     MscI     MslI   MspA1I     NspI    PshAI     PstI
    PvuII     SfcI     SmlI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaBI    BsaHI
    BsaWI     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI