Restriction map for
pF1KB7401
(Linear) (Six-base) MAPSORT of: KIBB7401.seq Check: 9000 from: 1 to: 591
KIBB7401.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 591
Size: 201 390
AfeI AGC'GCT
Cuts at: 0 175 591
Size: 175 416
AleI CACnn'nnGTG
Cuts at: 0 379 443 591
Size: 379 64 148
Fragments arranged by size:
379 148 64
ApaI G_GGCC'C
Cuts at: 0 199 591
Size: 199 392
BanI G'GyrC_C
Cuts at: 0 427 591
Size: 427 164
BanII G_rGCy'C
Cuts at: 0 199 307 455 532 591
Size: 199 108 148 77 59
Fragments arranged by size:
199 148 108 77 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 345 354 591
Size: 345 9 237
Fragments arranged by size:
345 237 9
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 490 524 591
Size: 490 34 67
Fragments arranged by size:
490 67 34
BlpI GC'TnA_GC
Cuts at: 0 142 591
Size: 142 449
Bme1580I G_kGCm'C
Cuts at: 0 199 430 591
Size: 199 231 161
Fragments arranged by size:
231 199 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 591
Size: 439 152
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 289 319 591
Size: 289 30 272
Fragments arranged by size:
289 272 30
BseYI C'CCAG_C
Cuts at: 0 474 558 591
Size: 474 84 33
Fragments arranged by size:
474 84 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 205 591
Size: 181 24 386
Fragments arranged by size:
386 181 24
BsiEI CG_ry'CG
Cuts at: 0 111 206 591
Size: 111 95 385
Fragments arranged by size:
385 111 95
BsiHKAI G_wGCw'C
Cuts at: 0 455 591
Size: 455 136
Bsp1286I G_dGCh'C
Cuts at: 0 199 307 430 455 532 591
Size: 199 108 123 25 77 59
Fragments arranged by size:
199 123 108 77 59 25
BsrDI GCAATG_nn'
Cuts at: 0 365 560 591
Size: 365 195 31
Fragments arranged by size:
365 195 31
BsrFI r'CCGG_y
Cuts at: 0 201 591
Size: 201 390
BssSI C'ACGA_G
Cuts at: 0 298 591
Size: 298 293
BtgI C'CryG_G
Cuts at: 0 105 591
Size: 105 486
DraIII CAC_nnn'GTG
Cuts at: 0 401 591
Size: 401 190
EaeI y'GGCC_r
Cuts at: 0 93 108 203 251 591
Size: 93 15 95 48 340
Fragments arranged by size:
340 95 93 48 15
EagI C'GGCC_G
Cuts at: 0 108 203 591
Size: 108 95 388
Fragments arranged by size:
388 108 95
EarI CTCTTCn'nnn_
Cuts at: 0 127 283 443 591
Size: 127 156 160 148
Fragments arranged by size:
160 156 148 127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 306 480 591
Size: 306 174 111
Fragments arranged by size:
306 174 111
EcoICRI GAG'CTC
Cuts at: 0 453 591
Size: 453 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 439 591
Size: 201 238 152
Fragments arranged by size:
238 201 152
EcoNI CCTnn'n_nnAGG
Cuts at: 0 289 591
Size: 289 302
EcoO109I rG'GnC_Cy
Cuts at: 0 195 591
Size: 195 396
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 565 591
Size: 565 26
HaeII r_GCGC'y
Cuts at: 0 7 149 177 344 591
Size: 7 142 28 167 247
Fragments arranged by size:
247 167 142 28 7
MfeI C'AATT_G
Cuts at: 0 512 591
Size: 512 79
MslI CAynn'nnrTG
Cuts at: 0 379 443 591
Size: 379 64 148
Fragments arranged by size:
379 148 64
MspA1I CmG'CkG
Cuts at: 0 81 107 113 443 591
Size: 81 26 6 330 148
Fragments arranged by size:
330 148 81 26 6
NaeI GCC'GGC
Cuts at: 0 203 591
Size: 203 388
NgoMIV G'CCGG_C
Cuts at: 0 201 591
Size: 201 390
NotI GC'GGCC_GC
Cuts at: 0 108 591
Size: 108 483
NspI r_CATG'y
Cuts at: 0 138 591
Size: 138 453
PfoI T'CCnGG_A
Cuts at: 0 455 591
Size: 455 136
PspOMI G'GGCC_C
Cuts at: 0 195 591
Size: 195 396
PvuII CAG'CTG
Cuts at: 0 443 591
Size: 443 148
SacI G_AGCT'C
Cuts at: 0 455 591
Size: 455 136
SacII CC_GC'GG
Cuts at: 0 108 591
Size: 108 483
SapI GCTCTTCn'nnn_
Cuts at: 0 127 283 443 591
Size: 127 156 160 148
Fragments arranged by size:
160 156 148 127
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 289 321 591
Size: 289 32 270
Fragments arranged by size:
289 270 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 384 591
Size: 384 207
Enzymes that do cut:
AcuI AfeI AleI ApaI BanI BanII BbsI BcgI
BlpI Bme1580I BpmI BsaXI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrDI BsrFI BssSI BtgI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
MfeI MslI MspA1I NaeI NgoMIV NotI NspI PfoI
PspOMI PvuII SacI SacII SapI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI NarI NcoI NdeI NheI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TatI Tth111I
XbaI XhoI XmaI XmnI ZraI