(Linear) (Six-base) MAPSORT of: KIBB7403.seq  Check: 9946  from: 1  to: 1470



KIBB7403.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     809    1186    1470 
   Size:        809     377     284

  Fragments arranged by size: 

                 809     377     284
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445    1124    1470 
   Size:        445     679     346

  Fragments arranged by size: 

                 679     445     346
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1040    1470 
   Size:       1040     430
 
AlwNI CAG_nnn'CTG

Cuts at:      0     962    1470 
   Size:        962     508
 
ApoI r'AATT_y

Cuts at:      0     202     555     606     746    1168    1470 
   Size:        202     353      51     140     422     302

  Fragments arranged by size: 

                 422     353     302     202     140      51
 
BamHI G'GATC_C

Cuts at:      0     577    1470 
   Size:        577     893
 
BanI G'GyrC_C

Cuts at:      0     853    1470 
   Size:        853     617
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     814    1470 
   Size:        814     656
 
Bme1580I G_kGCm'C

Cuts at:      0     459    1470 
   Size:        459    1011
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     654    1182    1470 
   Size:        654     528     288

  Fragments arranged by size: 

                 654     528     288
 
BsaBI GATnn'nnATC

Cuts at:      0     582    1470 
   Size:        582     888
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1100    1130    1470 
   Size:       1100      30     340

  Fragments arranged by size: 

                1100     340      30
 
BseYI C'CCAG_C

Cuts at:      0    1391    1470 
   Size:       1391      79
 
Bsp1286I G_dGCh'C

Cuts at:      0     459    1470 
   Size:        459    1011
 
BspHI T'CATG_A

Cuts at:      0     234    1470 
   Size:        234    1236
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     480     808    1008    1470 
   Size:        480     328     200     462

  Fragments arranged by size: 

                 480     462     328     200
 
BsrDI GCAATG_nn'

Cuts at:      0    1155    1470 
   Size:       1155     315
 
BsrFI r'CCGG_y

Cuts at:      0     485    1470 
   Size:        485     985
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     573    1470 
   Size:        573     897
 
BstYI r'GATC_y

Cuts at:      0     577    1470 
   Size:        577     893
 
BstZ17I GTA'TAC

Cuts at:      0     810    1470 
   Size:        810     660
 
Bsu36I CC'TnA_GG

Cuts at:      0     473    1470 
   Size:        473     997
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     863     898    1470 
   Size:        863      35     572

  Fragments arranged by size: 

                 863     572      35
 
DraI TTT'AAA

Cuts at:      0    1157    1470 
   Size:       1157     313
 
EaeI y'GGCC_r

Cuts at:      0     665    1470 
   Size:        665     805
 
EarI CTCTTCn'nnn_

Cuts at:      0    1256    1470 
   Size:       1256     214
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     445     654    1124    1182    1470 
   Size:        445     209     470      58     288

  Fragments arranged by size: 

                 470     445     288     209      58
 
EcoO109I rG'GnC_Cy

Cuts at:      0     306     469    1470 
   Size:        306     163    1001

  Fragments arranged by size: 

                1001     306     163
 
EcoRI G'AATT_C

Cuts at:      0    1168    1470 
   Size:       1168     302
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     562     594    1470 
   Size:        562      32     876

  Fragments arranged by size: 

                 876     562      32
 
HindIII A'AGCT_T

Cuts at:      0     660    1470 
   Size:        660     810
 
MfeI C'AATT_G

Cuts at:      0    1319    1470 
   Size:       1319     151
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     863    1470 
   Size:        863     607
 
MscI TGG'CCA

Cuts at:      0     667    1470 
   Size:        667     803
 
MslI CAynn'nnrTG

Cuts at:      0     239     713    1465    1470 
   Size:        239     474     752       5

  Fragments arranged by size: 

                 752     474     239       5
 
MspA1I CmG'CkG

Cuts at:      0     832    1470 
   Size:        832     638
 
NaeI GCC'GGC

Cuts at:      0     487    1470 
   Size:        487     983
 
NdeI CA'TA_TG

Cuts at:      0     714    1470 
   Size:        714     756
 
NgoMIV G'CCGG_C

Cuts at:      0     485    1470 
   Size:        485     985
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1410    1470 
   Size:       1410      60
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1422    1454    1470 
   Size:       1422      32      16

  Fragments arranged by size: 

                1422      32      16
 
PpuMI rG'GwC_Cy

Cuts at:      0     306     469    1470 
   Size:        306     163    1001

  Fragments arranged by size: 

                1001     306     163
 
PvuII CAG'CTG

Cuts at:      0     832    1470 
   Size:        832     638
 
ScaI AGT'ACT

Cuts at:      0    1218    1267    1470 
   Size:       1218      49     203

  Fragments arranged by size: 

                1218     203      49
 
SexAI A'CCwGG_T

Cuts at:      0     889    1470 
   Size:        889     581
 
SfcI C'TryA_G

Cuts at:      0      95     752     779     954    1269    1470 
   Size:         95     657      27     175     315     201

  Fragments arranged by size: 

                 657     315     201     175      95      27
 
SspI AAT'ATT

Cuts at:      0     266     394     513    1032    1470 
   Size:        266     128     119     519     438

  Fragments arranged by size: 

                 519     438     266     128     119
 
StyI C'CwwG_G

Cuts at:      0     877    1101    1470 
   Size:        877     224     369

  Fragments arranged by size: 

                 877     369     224
 
TatI w'GTAC_w

Cuts at:      0    1216    1265    1470 
   Size:       1216      49     205

  Fragments arranged by size: 

                1216     205      49
 
XmnI GAAnn'nnTTC

Cuts at:      0     206    1470 
   Size:        206    1264
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI    BamHI     BanI     BbsI
 Bme1580I     BpmI    BsaBI    BsaXI    BseYI Bsp1286I    BspHI    BspMI
    BsrDI    BsrFI    BstXI    BstYI  BstZ17I   Bsu36I    CspCI     DraI
     EaeI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I  HindIII     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NdeI   NgoMIV    PflMI
     PpiI    PpuMI    PvuII     ScaI    SexAI     SfcI     SspI     StyI
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII     BtgI    BtgZI
     BtsI     ClaI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MluI     NarI     NcoI     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI