(Linear) (Six-base) MAPSORT of: KIBB7406.seq  Check: 4588  from: 1  to: 657



KIBB7406.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:01  ..

AccI GT'mk_AC

Cuts at:      0     574     657 
   Size:        574      83
 
AflIII A'CryG_T

Cuts at:      0      83     657 
   Size:         83     574
 
AvaI C'yCGr_G

Cuts at:      0     150     657 
   Size:        150     507
 
BanI G'GyrC_C

Cuts at:      0      55     657 
   Size:         55     602
 
Bme1580I G_kGCm'C

Cuts at:      0      58     657 
   Size:         58     599
 
BmgBI CAC'GTC

Cuts at:      0      84     657 
   Size:         84     573
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     163     211     657 
   Size:         97      66      48     446

  Fragments arranged by size: 

                 446      97      66      48
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     657 
   Size:        121     536
 
BsaHI Gr'CG_yC

Cuts at:      0      17     657 
   Size:         17     640
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      70     100     657 
   Size:         70      30     557

  Fragments arranged by size: 

                 557      70      30
 
BseYI C'CCAG_C

Cuts at:      0     113     301     657 
   Size:        113     188     356

  Fragments arranged by size: 

                 356     188     113
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     657 
   Size:         58     599
 
BsrBI CCG'CTC

Cuts at:      0     195     228     657 
   Size:        195      33     429

  Fragments arranged by size: 

                 429     195      33
 
BsrFI r'CCGG_y

Cuts at:      0     131     657 
   Size:        131     526
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     606     657 
   Size:        606      51
 
BstYI r'GATC_y

Cuts at:      0      24     657 
   Size:         24     633
 
BtgI C'CryG_G

Cuts at:      0     246     657 
   Size:        246     411
 
BtsI GCAGTG_nn'

Cuts at:      0     540     657 
   Size:        540     117
 
DraIII CAC_nnn'GTG

Cuts at:      0     567     657 
   Size:        567      90
 
EaeI y'GGCC_r

Cuts at:      0     119     206     657 
   Size:        119      87     451

  Fragments arranged by size: 

                 451     119      87
 
EarI CTCTTCn'nnn_

Cuts at:      0     500     657 
   Size:        500     157
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     163     211     657 
   Size:         97      66      48     446

  Fragments arranged by size: 

                 446      97      66      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     514     657 
   Size:        514     143
 
EcoRV GAT'ATC

Cuts at:      0     315     657 
   Size:        315     342
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     222     254     657 
   Size:        222      32     403

  Fragments arranged by size: 

                 403     222      32
 
MscI TGG'CCA

Cuts at:      0     208     657 
   Size:        208     449
 
MspA1I CmG'CkG

Cuts at:      0     108     117     124     453     482     657 
   Size:        108       9       7     329      29     175

  Fragments arranged by size: 

                 329     175     108      29       9       7
 
NaeI GCC'GGC

Cuts at:      0     133     657 
   Size:        133     524
 
NgoMIV G'CCGG_C

Cuts at:      0     131     657 
   Size:        131     526
 
PshAI GACnn'nnGTC

Cuts at:      0     453     657 
   Size:        453     204
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     405     437     657 
   Size:        405      32     220

  Fragments arranged by size: 

                 405     220      32
 
PvuII CAG'CTG

Cuts at:      0     117     453     482     657 
   Size:        117     336      29     175

  Fragments arranged by size: 

                 336     175     117      29
 
SfcI C'TryA_G

Cuts at:      0     408     657 
   Size:        408     249
 
SmlI C'TyrA_G

Cuts at:      0     136     657 
   Size:        136     521
 
StyI C'CwwG_G

Cuts at:      0     215     517     657 
   Size:        215     302     140

  Fragments arranged by size: 

                 302     215     140
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     484     657 
   Size:        484     173
 

 Enzymes that do cut:

     AccI   AflIII     AvaI     BanI Bme1580I    BmgBI     BpmI    BpuEI
    BsaHI    BsaXI    BseYI Bsp1286I    BsrBI    BsrFI    BstXI    BstYI
     BtgI     BtsI   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRV
    Hin4I     MscI   MspA1I     NaeI   NgoMIV    PshAI     PsrI    PvuII
     SfcI     SmlI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaWI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI