Restriction map for
pF1KB7407
(Linear) (Six-base) MAPSORT of: KIBB7407.seq Check: 5434 from: 1 to: 639
KIBB7407.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AccI GT'mk_AC
Cuts at: 0 116 639
Size: 116 523
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 639
Size: 517 122
AfeI AGC'GCT
Cuts at: 0 331 639
Size: 331 308
AvaI C'yCGr_G
Cuts at: 0 358 579 639
Size: 358 221 60
Fragments arranged by size:
358 221 60
BanI G'GyrC_C
Cuts at: 0 3 231 354 639
Size: 3 228 123 285
Fragments arranged by size:
285 228 123 3
BanII G_rGCy'C
Cuts at: 0 548 639
Size: 548 91
BbeI G_GCGC'C
Cuts at: 0 7 235 358 639
Size: 7 228 123 281
Fragments arranged by size:
281 228 123 7
BbvCI CC'TCA_GC
Cuts at: 0 601 639
Size: 601 38
BglI GCCn_nnn'nGGC
Cuts at: 0 320 363 584 639
Size: 320 43 221 55
Fragments arranged by size:
320 221 55 43
Bpu10I CC'TnA_GC
Cuts at: 0 542 601 639
Size: 542 59 38
Fragments arranged by size:
542 59 38
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 639
Size: 355 284
BsaI GGTCTCn'nnnn_
Cuts at: 0 517 639
Size: 517 122
BsaAI yAC'GTr
Cuts at: 0 267 507 639
Size: 267 240 132
Fragments arranged by size:
267 240 132
BsaHI Gr'CG_yC
Cuts at: 0 4 83 232 355 639
Size: 4 79 149 123 284
Fragments arranged by size:
284 149 123 79 4
BseYI C'CCAG_C
Cuts at: 0 332 639
Size: 332 307
BsiEI CG_ry'CG
Cuts at: 0 248 514 639
Size: 248 266 125
Fragments arranged by size:
266 248 125
BsiHKAI G_wGCw'C
Cuts at: 0 263 620 639
Size: 263 357 19
Fragments arranged by size:
357 263 19
Bsp1286I G_dGCh'C
Cuts at: 0 263 548 620 639
Size: 263 285 72 19
Fragments arranged by size:
285 263 72 19
BsrBI CCG'CTC
Cuts at: 0 369 639
Size: 369 270
BsrFI r'CCGG_y
Cuts at: 0 243 344 479 639
Size: 243 101 135 160
Fragments arranged by size:
243 160 135 101
BsrGI T'GTAC_A
Cuts at: 0 272 639
Size: 272 367
BssHII G'CGCG_C
Cuts at: 0 70 72 639
Size: 70 2 567
Fragments arranged by size:
567 70 2
BssSI C'ACGA_G
Cuts at: 0 436 567 639
Size: 436 131 72
Fragments arranged by size:
436 131 72
BstAPI GCAn_nnn'nTGC
Cuts at: 0 267 639
Size: 267 372
BstXI CCAn_nnnn'nTGG
Cuts at: 0 474 639
Size: 474 165
BtgI C'CryG_G
Cuts at: 0 350 639
Size: 350 289
DraIII CAC_nnn'GTG
Cuts at: 0 267 639
Size: 267 372
EaeI y'GGCC_r
Cuts at: 0 245 432 511 639
Size: 245 187 79 128
Fragments arranged by size:
245 187 128 79
EagI C'GGCC_G
Cuts at: 0 245 511 639
Size: 245 266 128
Fragments arranged by size:
266 245 128
EarI CTCTTCn'nnn_
Cuts at: 0 400 601 639
Size: 400 201 38
Fragments arranged by size:
400 201 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 639
Size: 517 122
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 639
Size: 532 107
HaeII r_GCGC'y
Cuts at: 0 7 18 235 333 358 639
Size: 7 11 217 98 25 281
Fragments arranged by size:
281 217 98 25 11 7
HincII GTy'rAC
Cuts at: 0 282 639
Size: 282 357
KasI G'GCGC_C
Cuts at: 0 3 231 354 639
Size: 3 228 123 285
Fragments arranged by size:
285 228 123 3
MslI CAynn'nnrTG
Cuts at: 0 613 639
Size: 613 26
MspA1I CmG'CkG
Cuts at: 0 21 30 51 237 352 639
Size: 21 9 21 186 115 287
Fragments arranged by size:
287 186 115 21 21 9
NaeI GCC'GGC
Cuts at: 0 245 639
Size: 245 394
NarI GG'CG_CC
Cuts at: 0 4 232 355 639
Size: 4 228 123 284
Fragments arranged by size:
284 228 123 4
NgoMIV G'CCGG_C
Cuts at: 0 243 639
Size: 243 396
PasI CC'CwG_GG
Cuts at: 0 411 639
Size: 411 228
RsrII CG'GwC_CG
Cuts at: 0 102 639
Size: 102 537
SacII CC_GC'GG
Cuts at: 0 353 639
Size: 353 286
SapI GCTCTTCn'nnn_
Cuts at: 0 400 639
Size: 400 239
SfoI GGC'GCC
Cuts at: 0 5 233 356 639
Size: 5 228 123 283
Fragments arranged by size:
283 228 123 5
SmaI CCC'GGG
Cuts at: 0 360 581 639
Size: 360 221 58
Fragments arranged by size:
360 221 58
SmlI C'TyrA_G
Cuts at: 0 370 639
Size: 370 269
SrfI GCCC'GGGC
Cuts at: 0 581 639
Size: 581 58
TatI w'GTAC_w
Cuts at: 0 272 639
Size: 272 367
XmaI C'CCGG_G
Cuts at: 0 358 579 639
Size: 358 221 60
Fragments arranged by size:
358 221 60
Enzymes that do cut:
AccI AcuI AfeI AvaI BanI BanII BbeI BbvCI
BglI Bpu10I BpuEI BsaI BsaAI BsaHI BseYI BsiEI
BsiHKAI Bsp1286I BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstXI BtgI DraIII EaeI EagI EarI Eco57MI EcoNI
HaeII HincII KasI MslI MspA1I NaeI NarI NgoMIV
PasI RsrII SacII SapI SfoI SmaI SmlI SrfI
TatI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI
BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BsaBI BsaWI BsaXI BseRI BsgI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrDI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
MmeI MscI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SnaBI SpeI SphI SspI StuI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmnI ZraI