Restriction map for
pF1KB7408
(Linear) (Six-base) MAPSORT of: KIBB7408.seq Check: 6262 from: 1 to: 1182
KIBB7408.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
Acc65I G'GTAC_C
Cuts at: 0 395 1182
Size: 395 787
AleI CACnn'nnGTG
Cuts at: 0 404 493 1182
Size: 404 89 689
Fragments arranged by size:
689 404 89
ApoI r'AATT_y
Cuts at: 0 552 895 918 1182
Size: 552 343 23 264
Fragments arranged by size:
552 343 264 23
AseI AT'TA_AT
Cuts at: 0 787 1182
Size: 787 395
BanI G'GyrC_C
Cuts at: 0 191 395 1182
Size: 191 204 787
Fragments arranged by size:
787 204 191
BanII G_rGCy'C
Cuts at: 0 392 1182
Size: 392 790
BbsI GAAGACnn'nnnn_
Cuts at: 0 169 280 1182
Size: 169 111 902
Fragments arranged by size:
902 169 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 136 170 247 281 1182
Size: 136 34 77 34 901
Fragments arranged by size:
901 136 77 34 34
BglII A'GATC_T
Cuts at: 0 414 1032 1182
Size: 414 618 150
Fragments arranged by size:
618 414 150
Bme1580I G_kGCm'C
Cuts at: 0 133 1182
Size: 133 1049
BmgBI CAC'GTC
Cuts at: 0 402 495 1182
Size: 402 93 687
Fragments arranged by size:
687 402 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 542 641 1182
Size: 542 99 541
Fragments arranged by size:
542 541 99
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 269 1182
Size: 158 111 913
Fragments arranged by size:
913 158 111
Bpu10I CC'TnA_GC
Cuts at: 0 481 1182
Size: 481 701
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1013 1024 1149 1182
Size: 1013 11 125 33
Fragments arranged by size:
1013 125 33 11
BsaI GGTCTCn'nnnn_
Cuts at: 0 61 134 245 1182
Size: 61 73 111 937
Fragments arranged by size:
937 111 73 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 379 409 1182
Size: 379 30 773
Fragments arranged by size:
773 379 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 742 1182
Size: 742 440
Bsp1286I G_dGCh'C
Cuts at: 0 133 392 1182
Size: 133 259 790
Fragments arranged by size:
790 259 133
BsrBI CCG'CTC
Cuts at: 0 113 335 1182
Size: 113 222 847
Fragments arranged by size:
847 222 113
BsrFI r'CCGG_y
Cuts at: 0 24 1182
Size: 24 1158
BsrGI T'GTAC_A
Cuts at: 0 867 1182
Size: 867 315
BstAPI GCAn_nnn'nTGC
Cuts at: 0 80 191 302 1182
Size: 80 111 111 880
Fragments arranged by size:
880 111 111 80
BstXI CCAn_nnnn'nTGG
Cuts at: 0 406 1182
Size: 406 776
BstYI r'GATC_y
Cuts at: 0 414 465 1032 1182
Size: 414 51 567 150
Fragments arranged by size:
567 414 150 51
BtgI C'CryG_G
Cuts at: 0 405 1182
Size: 405 777
BtsI GCAGTG_nn'
Cuts at: 0 198 309 799 1182
Size: 198 111 490 383
Fragments arranged by size:
490 383 198 111
DraI TTT'AAA
Cuts at: 0 923 1182
Size: 923 259
EaeI y'GGCC_r
Cuts at: 0 791 1049 1182
Size: 791 258 133
Fragments arranged by size:
791 258 133
EarI CTCTTCn'nnn_
Cuts at: 0 39 1140 1182
Size: 39 1101 42
Fragments arranged by size:
1101 42 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 269 1182
Size: 158 111 913
Fragments arranged by size:
913 158 111
EcoNI CCTnn'n_nnAGG
Cuts at: 0 102 213 324 1182
Size: 102 111 111 858
Fragments arranged by size:
858 111 111 102
EcoRI G'AATT_C
Cuts at: 0 552 1182
Size: 552 630
EcoRV GAT'ATC
Cuts at: 0 822 1182
Size: 822 360
HaeII r_GCGC'y
Cuts at: 0 245 356 380 1182
Size: 245 111 24 802
Fragments arranged by size:
802 245 111 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 811 843 1182
Size: 811 32 339
Fragments arranged by size:
811 339 32
HincII GTy'rAC
Cuts at: 0 592 1182
Size: 592 590
KpnI G_GTAC'C
Cuts at: 0 399 1182
Size: 399 783
MscI TGG'CCA
Cuts at: 0 793 1182
Size: 793 389
MslI CAynn'nnrTG
Cuts at: 0 404 475 493 1079 1182
Size: 404 71 18 586 103
Fragments arranged by size:
586 404 103 71 18
NaeI GCC'GGC
Cuts at: 0 26 1182
Size: 26 1156
NgoMIV G'CCGG_C
Cuts at: 0 24 1182
Size: 24 1158
PstI C_TGCA'G
Cuts at: 0 217 328 1182
Size: 217 111 854
Fragments arranged by size:
854 217 111
SfcI C'TryA_G
Cuts at: 0 102 213 324 1182
Size: 102 111 111 858
Fragments arranged by size:
858 111 111 102
SmlI C'TyrA_G
Cuts at: 0 1003 1028 1164 1182
Size: 1003 25 136 18
Fragments arranged by size:
1003 136 25 18
SspI AAT'ATT
Cuts at: 0 729 1014 1182
Size: 729 285 168
Fragments arranged by size:
729 285 168
SwaI ATTT'AAAT
Cuts at: 0 923 1182
Size: 923 259
TatI w'GTAC_w
Cuts at: 0 867 1182
Size: 867 315
Tth111I GACn'n_nGTC
Cuts at: 0 587 1182
Size: 587 595
Enzymes that do cut:
Acc65I AleI ApoI AseI BanI BanII BbsI BcgI
BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BsgI Bsp1286I BsrBI BsrFI BsrGI BstAPI BstXI
BstYI BtgI BtsI DraI EaeI EarI Eco57MI EcoNI
EcoRI EcoRV HaeII Hin4I HincII KpnI MscI MslI
NaeI NgoMIV PstI SfcI SmlI SspI SwaI TatI
Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BciVI
BclI BfrBI BglI BlpI BmrI BmtI BsaAI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI EcoO109I FalI FseI FspI FspAI
HindIII HpaI KasI MfeI MluI MmeI MspA1I NarI
NcoI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI StyI TaqII TstI XbaI XcmI XhoI
XmaI XmnI ZraI