Restriction map for
pF1KB7410
(Linear) (Six-base) MAPSORT of: KIBB7410.seq Check: 7742 from: 1 to: 1152
KIBB7410.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 813 1152
Size: 813 339
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 638 1152
Size: 638 514
AflIII A'CryG_T
Cuts at: 0 646 1152
Size: 646 506
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 1152
Size: 1036 32 84
Fragments arranged by size:
1036 84 32
AlwNI CAG_nnn'CTG
Cuts at: 0 147 1152
Size: 147 1005
ApoI r'AATT_y
Cuts at: 0 502 1152
Size: 502 650
AvaI C'yCGr_G
Cuts at: 0 9 1152
Size: 9 1143
BanI G'GyrC_C
Cuts at: 0 14 539 1152
Size: 14 525 613
Fragments arranged by size:
613 525 14
BanII G_rGCy'C
Cuts at: 0 194 316 1152
Size: 194 122 836
Fragments arranged by size:
836 194 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 111 236 783 1152
Size: 111 125 547 369
Fragments arranged by size:
547 369 125 111
BciVI GTATCCnnnnn_n'
Cuts at: 0 430 975 1152
Size: 430 545 177
Fragments arranged by size:
545 430 177
BglII A'GATC_T
Cuts at: 0 688 1152
Size: 688 464
Bme1580I G_kGCm'C
Cuts at: 0 17 544 1152
Size: 17 527 608
Fragments arranged by size:
608 527 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1152
Size: 232 920
Bpu10I CC'TnA_GC
Cuts at: 0 310 1152
Size: 310 842
BsaI GGTCTCn'nnnn_
Cuts at: 0 12 1152
Size: 12 1140
BsaBI GATnn'nnATC
Cuts at: 0 607 1152
Size: 607 545
BsaWI w'CCGG_w
Cuts at: 0 567 1152
Size: 567 585
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 230 260 904 934 1036 1066 1152
Size: 230 30 644 30 102 30 86
Fragments arranged by size:
644 230 102 86 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 202 517 759 1152
Size: 202 315 242 393
Fragments arranged by size:
393 315 242 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 315 908 1152
Size: 315 593 244
Fragments arranged by size:
593 315 244
BsiHKAI G_wGCw'C
Cuts at: 0 194 316 1152
Size: 194 122 836
Fragments arranged by size:
836 194 122
BsmI GAATG_Cn'
Cuts at: 0 939 1152
Size: 939 213
Bsp1286I G_dGCh'C
Cuts at: 0 17 194 316 544 1152
Size: 17 177 122 228 608
Fragments arranged by size:
608 228 177 122 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 813 1152
Size: 813 339
BsrDI GCAATG_nn'
Cuts at: 0 854 1055 1152
Size: 854 201 97
Fragments arranged by size:
854 201 97
BssSI C'ACGA_G
Cuts at: 0 753 1152
Size: 753 399
BstEII G'GTnAC_C
Cuts at: 0 90 1152
Size: 90 1062
BstYI r'GATC_y
Cuts at: 0 243 688 1152
Size: 243 445 464
Fragments arranged by size:
464 445 243
BtgI C'CryG_G
Cuts at: 0 347 737 1152
Size: 347 390 415
Fragments arranged by size:
415 390 347
BtsI GCAGTG_nn'
Cuts at: 0 219 715 1152
Size: 219 496 437
Fragments arranged by size:
496 437 219
DrdI GACnn_nn'nnGTC
Cuts at: 0 142 1152
Size: 142 1010
EaeI y'GGCC_r
Cuts at: 0 138 1152
Size: 138 1014
EarI CTCTTCn'nnn_
Cuts at: 0 64 559 614 1152
Size: 64 495 55 538
Fragments arranged by size:
538 495 64 55
EcoICRI GAG'CTC
Cuts at: 0 192 314 1152
Size: 192 122 838
Fragments arranged by size:
838 192 122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 638 1152
Size: 232 406 514
Fragments arranged by size:
514 406 232
EcoO109I rG'GnC_Cy
Cuts at: 0 45 144 631 1152
Size: 45 99 487 521
Fragments arranged by size:
521 487 99 45
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 795 815 902 1017 1152
Size: 795 20 87 115 135
Fragments arranged by size:
795 135 115 87 20
MspA1I CmG'CkG
Cuts at: 0 892 1152
Size: 892 260
NcoI C'CATG_G
Cuts at: 0 737 1152
Size: 737 415
NspI r_CATG'y
Cuts at: 0 402 650 1152
Size: 402 248 502
Fragments arranged by size:
502 402 248
PasI CC'CwG_GG
Cuts at: 0 48 1152
Size: 48 1104
PciI A'CATG_T
Cuts at: 0 646 1152
Size: 646 506
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 299 1152
Size: 147 152 853
Fragments arranged by size:
853 152 147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 1152
Size: 1036 32 84
Fragments arranged by size:
1036 84 32
PpuMI rG'GwC_Cy
Cuts at: 0 144 1152
Size: 144 1008
PsiI TTA'TAA
Cuts at: 0 488 1152
Size: 488 664
PstI C_TGCA'G
Cuts at: 0 199 214 263 670 1152
Size: 199 15 49 407 482
Fragments arranged by size:
482 407 199 49 15
PvuII CAG'CTG
Cuts at: 0 892 1152
Size: 892 260
SacI G_AGCT'C
Cuts at: 0 194 316 1152
Size: 194 122 836
Fragments arranged by size:
836 194 122
SapI GCTCTTCn'nnn_
Cuts at: 0 64 614 1152
Size: 64 550 538
Fragments arranged by size:
550 538 64
ScaI AGT'ACT
Cuts at: 0 391 1152
Size: 391 761
SfcI C'TryA_G
Cuts at: 0 195 210 259 666 1152
Size: 195 15 49 407 486
Fragments arranged by size:
486 407 195 49 15
SphI G_CATG'C
Cuts at: 0 402 1152
Size: 402 750
StyI C'CwwG_G
Cuts at: 0 737 1152
Size: 737 415
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 1152
Size: 97 1055
TatI w'GTAC_w
Cuts at: 0 389 1152
Size: 389 763
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 809 841 1152
Size: 809 32 311
Fragments arranged by size:
809 311 32
XmnI GAAnn'nnTTC
Cuts at: 0 623 937 1152
Size: 623 314 215
Fragments arranged by size:
623 314 215
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApoI AvaI BanI
BanII BbsI BciVI BglII Bme1580I BpmI Bpu10I BsaI
BsaBI BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrDI BssSI BstEII BstYI BtgI BtsI DrdI
EaeI EarI EcoICRI Eco57MI EcoO109I MmeI MspA1I NcoI
NspI PasI PciI PflMI PpiI PpuMI PsiI PstI
PvuII SacI SapI ScaI SfcI SphI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI BsaHI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MscI MslI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PshAI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI