Restriction map for
pF1KB7411
(Linear) (Six-base) MAPSORT of: KIBB7411.seq Check: 4188 from: 1 to: 942
KIBB7411.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 602 942
Size: 602 340
AccI GT'mk_AC
Cuts at: 0 547 942
Size: 547 395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 942
Size: 58 884
AleI CACnn'nnGTG
Cuts at: 0 325 942
Size: 325 617
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 409 942
Size: 409 533
ApoI r'AATT_y
Cuts at: 0 166 942
Size: 166 776
BanI G'GyrC_C
Cuts at: 0 704 942
Size: 704 238
BanII G_rGCy'C
Cuts at: 0 35 942
Size: 35 907
BbeI G_GCGC'C
Cuts at: 0 708 942
Size: 708 234
BclI T'GATC_A
Cuts at: 0 140 641 942
Size: 140 501 301
Fragments arranged by size:
501 301 140
Bme1580I G_kGCm'C
Cuts at: 0 432 942
Size: 432 510
BmgBI CAC'GTC
Cuts at: 0 894 942
Size: 894 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 942
Size: 271 671
BsaHI Gr'CG_yC
Cuts at: 0 705 942
Size: 705 237
BsaWI w'CCGG_w
Cuts at: 0 497 942
Size: 497 445
BseYI C'CCAG_C
Cuts at: 0 442 942
Size: 442 500
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 303 942
Size: 303 639
Bsp1286I G_dGCh'C
Cuts at: 0 35 432 942
Size: 35 397 510
Fragments arranged by size:
510 397 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 602 942
Size: 602 340
BsrGI T'GTAC_A
Cuts at: 0 631 942
Size: 631 311
BstZ17I GTA'TAC
Cuts at: 0 548 942
Size: 548 394
Bsu36I CC'TnA_GG
Cuts at: 0 880 942
Size: 880 62
BtgI C'CryG_G
Cuts at: 0 759 942
Size: 759 183
BtsI GCAGTG_nn'
Cuts at: 0 320 359 942
Size: 320 39 583
Fragments arranged by size:
583 320 39
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 520 555 942
Size: 520 35 387
Fragments arranged by size:
520 387 35
EaeI y'GGCC_r
Cuts at: 0 377 407 942
Size: 377 30 535
Fragments arranged by size:
535 377 30
EarI CTCTTCn'nnn_
Cuts at: 0 91 231 942
Size: 91 140 711
Fragments arranged by size:
711 140 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 942
Size: 58 884
HaeII r_GCGC'y
Cuts at: 0 708 942
Size: 708 234
HincII GTy'rAC
Cuts at: 0 849 942
Size: 849 93
HindIII A'AGCT_T
Cuts at: 0 70 942
Size: 70 872
KasI G'GCGC_C
Cuts at: 0 704 942
Size: 704 238
MscI TGG'CCA
Cuts at: 0 379 409 942
Size: 379 30 533
Fragments arranged by size:
533 379 30
MslI CAynn'nnrTG
Cuts at: 0 178 325 562 670 942
Size: 178 147 237 108 272
Fragments arranged by size:
272 237 178 147 108
NarI GG'CG_CC
Cuts at: 0 705 942
Size: 705 237
NcoI C'CATG_G
Cuts at: 0 759 942
Size: 759 183
NspI r_CATG'y
Cuts at: 0 465 942
Size: 465 477
ScaI AGT'ACT
Cuts at: 0 307 942
Size: 307 635
SfcI C'TryA_G
Cuts at: 0 873 942
Size: 873 69
SfoI GGC'GCC
Cuts at: 0 706 942
Size: 706 236
SmlI C'TyrA_G
Cuts at: 0 250 942
Size: 250 692
StyI C'CwwG_G
Cuts at: 0 132 473 759 942
Size: 132 341 286 183
Fragments arranged by size:
341 286 183 132
TatI w'GTAC_w
Cuts at: 0 182 305 631 678 942
Size: 182 123 326 47 264
Fragments arranged by size:
326 264 182 123 47
Tth111I GACn'n_nGTC
Cuts at: 0 336 942
Size: 336 606
Enzymes that do cut:
AarI AccI AcuI AleI AlfI ApoI BanI BanII
BbeI BclI Bme1580I BmgBI BpuEI BsaHI BsaWI BseYI
BsgI Bsp1286I BspMI BsrGI BstZ17I Bsu36I BtgI BtsI
CspCI EaeI EarI Eco57MI HaeII HincII HindIII KasI
MscI MslI NarI NcoI NspI ScaI SfcI SfoI
SmlI StyI TatI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmrI BmtI BplI BpmI Bpu10I
BsaI BsaAI BsaBI BsaXI BseRI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgZI ClaI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HpaI
KpnI MfeI MluI MmeI MspA1I NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI XbaI XcmI XhoI XmaI XmnI ZraI