Restriction map for
pF1KB7412
(Linear) (Six-base) MAPSORT of: KIBB7412.seq Check: 5852 from: 1 to: 1017
KIBB7412.orf
With 182 enzymes: SgfI *
March 3, 2008 11:01 ..
AatII G_ACGT'C
Cuts at: 0 845 1017
Size: 845 172
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 878 995 1017
Size: 377 501 117 22
Fragments arranged by size:
501 377 117 22
AleI CACnn'nnGTG
Cuts at: 0 29 1017
Size: 29 988
ApoI r'AATT_y
Cuts at: 0 123 692 726 879 942 1017
Size: 123 569 34 153 63 75
Fragments arranged by size:
569 153 123 75 63 34
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 744 777 1017
Size: 744 33 240
Fragments arranged by size:
744 240 33
BamHI G'GATC_C
Cuts at: 0 786 1017
Size: 786 231
BanI G'GyrC_C
Cuts at: 0 142 288 470 924 955 1017
Size: 142 146 182 454 31 62
Fragments arranged by size:
454 182 146 142 62 31
BanII G_rGCy'C
Cuts at: 0 363 1017
Size: 363 654
BbeI G_GCGC'C
Cuts at: 0 146 292 474 1017
Size: 146 146 182 543
Fragments arranged by size:
543 182 146 146
BbsI GAAGACnn'nnnn_
Cuts at: 0 769 1017
Size: 769 248
BciVI GTATCCnnnnn_n'
Cuts at: 0 772 1017
Size: 772 245
Bme1580I G_kGCm'C
Cuts at: 0 259 927 1017
Size: 259 668 90
Fragments arranged by size:
668 259 90
BmrI ACTGGGnnnn_n'
Cuts at: 0 48 232 1016 1017
Size: 48 184 784 1
Fragments arranged by size:
784 184 48 1
BmtI G_CTAG'C
Cuts at: 0 236 1017
Size: 236 781
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 614 1017
Size: 614 403
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 869 1017
Size: 622 247 148
Fragments arranged by size:
622 247 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 843 1017
Size: 843 174
BsaHI Gr'CG_yC
Cuts at: 0 143 289 471 842 1017
Size: 143 146 182 371 175
Fragments arranged by size:
371 182 175 146 143
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 595 625 1017
Size: 595 30 392
Fragments arranged by size:
595 392 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 1017
Size: 55 962
BseYI C'CCAG_C
Cuts at: 0 7 1017
Size: 7 1010
BsiEI CG_ry'CG
Cuts at: 0 17 191 1017
Size: 17 174 826
Fragments arranged by size:
826 174 17
BsiHKAI G_wGCw'C
Cuts at: 0 323 430 1017
Size: 323 107 587
Fragments arranged by size:
587 323 107
Bsp1286I G_dGCh'C
Cuts at: 0 259 323 363 430 927 1017
Size: 259 64 40 67 497 90
Fragments arranged by size:
497 259 90 67 64 40
BsrFI r'CCGG_y
Cuts at: 0 28 1017
Size: 28 989
BssHII G'CGCG_C
Cuts at: 0 209 1017
Size: 209 808
BstXI CCAn_nnnn'nTGG
Cuts at: 0 666 1017
Size: 666 351
BstYI r'GATC_y
Cuts at: 0 786 1017
Size: 786 231
BtgI C'CryG_G
Cuts at: 0 203 1017
Size: 203 814
BtsI GCAGTG_nn'
Cuts at: 0 485 946 1017
Size: 485 461 71
Fragments arranged by size:
485 461 71
EaeI y'GGCC_r
Cuts at: 0 197 1017
Size: 197 820
EarI CTCTTCn'nnn_
Cuts at: 0 497 561 1017
Size: 497 64 456
Fragments arranged by size:
497 456 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 738 1017
Size: 738 279
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 622 869 878 995 1017
Size: 377 245 247 9 117 22
Fragments arranged by size:
377 247 245 117 22 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 526 1017
Size: 526 491
EcoO109I rG'GnC_Cy
Cuts at: 0 461 1017
Size: 461 556
EcoRI G'AATT_C
Cuts at: 0 726 879 1017
Size: 726 153 138
Fragments arranged by size:
726 153 138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 793 1017
Size: 793 224
HaeII r_GCGC'y
Cuts at: 0 146 292 400 474 1017
Size: 146 146 108 74 543
Fragments arranged by size:
543 146 146 108 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 778 810 1017
Size: 778 32 207
Fragments arranged by size:
778 207 32
KasI G'GCGC_C
Cuts at: 0 142 288 470 1017
Size: 142 146 182 547
Fragments arranged by size:
547 182 146 142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1017
Size: 776 241
MscI TGG'CCA
Cuts at: 0 199 1017
Size: 199 818
MslI CAynn'nnrTG
Cuts at: 0 29 1017
Size: 29 988
MspA1I CmG'CkG
Cuts at: 0 79 205 1017
Size: 79 126 812
Fragments arranged by size:
812 126 79
NarI GG'CG_CC
Cuts at: 0 143 289 471 1017
Size: 143 146 182 546
Fragments arranged by size:
546 182 146 143
NheI G'CTAG_C
Cuts at: 0 232 1017
Size: 232 785
NspI r_CATG'y
Cuts at: 0 513 1017
Size: 513 504
PfoI T'CCnGG_A
Cuts at: 0 908 1017
Size: 908 109
PpuMI rG'GwC_Cy
Cuts at: 0 461 1017
Size: 461 556
PstI C_TGCA'G
Cuts at: 0 164 1017
Size: 164 853
SacII CC_GC'GG
Cuts at: 0 206 1017
Size: 206 811
SapI GCTCTTCn'nnn_
Cuts at: 0 561 1017
Size: 561 456
SfcI C'TryA_G
Cuts at: 0 160 1017
Size: 160 857
SfoI GGC'GCC
Cuts at: 0 144 290 472 1017
Size: 144 146 182 545
Fragments arranged by size:
545 182 146 144
SgrAI Cr'CCGG_yG
Cuts at: 0 28 1017
Size: 28 989
SmlI C'TyrA_G
Cuts at: 0 858 1017
Size: 858 159
SphI G_CATG'C
Cuts at: 0 513 1017
Size: 513 504
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 386 659 1017
Size: 386 273 358
Fragments arranged by size:
386 358 273
TatI w'GTAC_w
Cuts at: 0 999 1017
Size: 999 18
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 477 509 1017
Size: 477 32 508
Fragments arranged by size:
508 477 32
ZraI GAC'GTC
Cuts at: 0 843 1017
Size: 843 174
Enzymes that do cut:
AatII AcuI AleI ApoI BaeI BamHI BanI BanII
BbeI BbsI BciVI Bme1580I BmrI BmtI BplI BpmI
BpuEI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BsrFI BssHII BstXI BstYI BtgI BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NarI NheI NspI
PfoI PpuMI PstI SacII SapI SfcI SfoI SgrAI
SmlI SphI TaqII TatI TstI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BbvCI BcgI BclI BfrBI BglI
BglII BlpI BmgBI Bpu10I BsaI BsaAI BsaBI BsaWI
BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NaeI NcoI NdeI NgoMIV NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SalI SanDI SbfI ScaI SexAI SfiI SgfI
SmaI SnaBI SpeI SrfI SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI